Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,177,313 +A 100% coding (64/990 nt) rbsB ← ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,177,3131.→A100.0% 1612.4 409coding (64/990 nt)rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (237/170):  ref base (1/1):  total (238/171)

Only 100 of 442 total aligned reads displayed.

CAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA...GGGTTG..GTCGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGG.CCCCAACAGGGCCAG.TTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCG  >  NC_014034/2177218‑2177404
                                                                                                      |                                                                                              
cAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCg                                                                                                 <  1:5159586/101‑1 (MQ=255)
cAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCg                                                                                                 >  2:739596/1‑101 (MQ=255)
cAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTCCGGCCg                                                                                                 >  1:276395/1‑101 (MQ=255)
 aGCCCCATCTCGGTCGCCTTTGCCGGAGCACCCTCCTGCATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.a                                                                                               <  2:4262452/101‑1 (MQ=255)
   ccccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aag                                                                                             <  1:2580809/101‑3 (MQ=255)
    cccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aagc                                                                                            <  1:315735/101‑4 (MQ=255)
      cATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aagccg                                                                                          >  2:3126977/1‑96 (MQ=255)
         ctcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGGTGGT.GTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGccc                                                                                        >  2:5624944/1‑101 (MQ=255)
         ctcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGccc                                                                                        >  1:6783391/1‑101 (MQ=255)
         ctcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGccc                                                                                        >  2:7704397/1‑101 (MQ=255)
          tcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGcccc                                                                                       <  2:3688412/101‑1 (MQ=255)
             gTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGc                                                                                    >  1:5791755/1‑101 (MQ=255)
              tCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGcc                                                                                   <  1:1021478/101‑1 (MQ=255)
              gcGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGcc                                                                                   <  2:519833/100‑1 (MQ=255)
               caccTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCa                                                                                  <  2:6013891/99‑1 (MQ=255)
               cGCCTTTGCCGAAGCCCCTTCCTTCATCTGGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCa                                                                                  <  1:1072412/101‑1 (MQ=255)
               cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCa                                                                                  >  2:5348223/1‑101 (MQ=255)
                  cTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgcg                                                                               >  2:4727197/1‑101 (MQ=255)
                  cTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgcg                                                                               >  1:4210195/1‑101 (MQ=255)
                   tttGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgcgc                                                                              >  1:3531666/1‑101 (MQ=255)
                   tttGCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgcgc                                                                              <  1:3801231/101‑1 (MQ=255)
                      gCCGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGa                                                                           >  1:5952372/1‑101 (MQ=255)
                       ccGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaa                                                                          <  1:6619669/101‑1 (MQ=255)
                       ccGAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaa                                                                          >  1:460224/1‑101 (MQ=255)
                         gAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGataa                                                                        >  2:9103627/1‑101 (MQ=255)
                         gAAGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaaa                                                                        >  2:113610/1‑101 (MQ=255)
                          aaGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAAc                                                                       >  1:4955115/1‑101 (MQ=255)
                          aaGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAAc                                                                       <  2:388257/101‑1 (MQ=255)
                          aaGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAAc                                                                       >  2:6484777/1‑101 (MQ=255)
                           aGCACCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACg                                                                      >  1:8787805/1‑101 (MQ=255)
                              cccttccttCATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCa                                                                   <  2:7104649/100‑1 (MQ=255)
                              aCCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCa                                                                   >  1:8527364/1‑101 (MQ=255)
                              aCCTTCCTTCATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCAGCCCGGGCCGCCGCGGAAAACGcag                                                                   >  2:1349908/1‑99 (MQ=255)
                                cttccttcATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gg                                                                >  2:5431504/1‑101 (MQ=255)
                                cttccttcATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gg                                                                >  2:2786333/1‑101 (MQ=255)
                                 ttccttcATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.ggg                                                               >  1:8963373/1‑100 (MQ=255)
                                  tccttcATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggc                                                              >  2:4593348/1‑99 (MQ=255)
                                  tccttcATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggc                                                              <  1:2908007/101‑3 (MQ=255)
                                  tccttcATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggc                                                              >  2:6155784/1‑99 (MQ=255)
                                   ccttcATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggca                                                             >  1:2815683/1‑98 (MQ=255)
                                   ccttcATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggca                                                             >  2:677859/1‑98 (MQ=255)
                                   ccttcATCTTGACGAAAAAG.GGGTTGGCGTCGGTCTTGGTGATCAGACAGCCCCCGGTTTCGGCCGAAGCCGCCACGGCCCGCGCGGAAAACGCCA.gggca                                                             >  1:4445495/1‑98 (MQ=255)
                                     ttcATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGcagatcggaa                                                            >  2:1611827/1‑92 (MQ=255)
                                     ttcATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaga                                                           <  1:244413/101‑6 (MQ=255)
                                     ttcATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaga                                                           >  1:1461332/1‑96 (MQ=255)
                                      tcATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCagag                                                           >  2:7347883/1‑101 (MQ=255)
                                      tcATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCagag                                                           >  2:7071974/1‑101 (MQ=255)
                                      tcATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCagag                                                           >  1:5101151/1‑101 (MQ=255)
                                      tcATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCagag                                                           <  1:7704723/101‑1 (MQ=255)
                                      tcATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCagag                                                           >  1:8223526/1‑101 (MQ=255)
                                      tcATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCagag                                                           <  2:2980233/101‑1 (MQ=255)
                                       cATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg                                                          <  2:8085820/101‑1 (MQ=255)
                                       cATCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg                                                          >  2:6712324/1‑101 (MQ=255)
                                         tCTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.cc                                                       >  1:7811646/1‑101 (MQ=255)
                                          cTTGACGAAAAAG.GGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.ccc                                                      >  2:5708748/1‑101 (MQ=255)
                                                       ggggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCa                                           <  1:1958300/92‑1 (MQ=255)
                                                       ggggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCa                                           <  2:6365038/92‑1 (MQ=255)
                                                       ggggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCa                                           <  1:6421301/92‑1 (MQ=255)
                                                         ggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.t                                        >  2:8424355/8‑101 (MQ=255)
                                                          gttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tt                                       <  1:7123026/95‑1 (MQ=255)
                                                            tggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttg                                     >  2:3359062/5‑99 (MQ=255)
                                                            tggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttg                                     <  1:6156021/97‑3 (MQ=255)
                                                             ggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgc                                    <  2:7851899/98‑4 (MQ=255)
                                                                gtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGca                                  >  2:8582760/1‑101 (MQ=255)
                                                                gtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGca                                  >  1:6562079/1‑101 (MQ=255)
                                                                  cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGcatc                                <  2:3269050/101‑1 (MQ=255)
                                                                  cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGcatc                                <  2:8290234/101‑1 (MQ=255)
                                                                  cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGcatc                                <  1:739609/101‑1 (MQ=255)
                                                                  cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGcatc                                >  2:9015895/1‑101 (MQ=255)
                                                                      cttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCAtcatca                            >  1:914632/10‑101 (MQ=255)
                                                                       ttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCAc                           >  2:6304195/9‑101 (MQ=255)
                                                                       tgggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCAc                           <  2:2238598/93‑1 (MQ=255)
                                                                        tggtgatcagacagGCCCCGGTTTCGGCCGAAGGCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACg                          >  1:4778638/8‑101 (MQ=255)
                                                                        tggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACg                          >  1:4015905/8‑101 (MQ=255)
                                                                        tggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACg                          <  1:62672/94‑1 (MQ=255)
                                                                         ggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACgg                         >  1:5297882/7‑101 (MQ=255)
                                                                         ggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACgg                         >  2:6966105/7‑101 (MQ=255)
                                                                            gatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtct                      >  2:3828672/4‑101 (MQ=255)
                                                                            gatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtct                      <  1:3274474/98‑1 (MQ=255)
                                                                            gatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtcc                      >  1:2091588/4‑100 (MQ=255)
                                                                             atcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtctc                     <  2:5100982/99‑1 (MQ=255)
                                                                               cagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtctctc                   <  2:1708639/101‑1 (MQ=255)
                                                                                 gacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTctcct                 >  1:1318331/1‑101 (MQ=255)
                                                                                 gacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTctcct                 <  2:2444234/101‑1 (MQ=255)
                                                                                  acagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTctcctc                >  2:1262809/1‑101 (MQ=255)
                                                                                   cagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCtcctcc               >  1:7279586/1‑101 (MQ=255)
                                                                                    agGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTccc              <  1:2302917/101‑1 (MQ=255)
                                                                                        cccGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGttt          <  1:4310586/101‑1 (MQ=255)
                                                                                        cccGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGttt          <  2:401375/101‑1 (MQ=255)
                                                                                         ccGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTc         <  2:7531611/101‑1 (MQ=255)
                                                                                          cGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCa        <  2:4120461/101‑1 (MQ=255)
                                                                                          cGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCa        <  1:3446956/101‑1 (MQ=255)
                                                                                          cGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCa        <  1:3055413/101‑1 (MQ=255)
                                                                                           ggTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCAc       >  2:4562161/1‑101 (MQ=255)
                                                                                           ggTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCAc       >  1:7083506/1‑101 (MQ=255)
                                                                                           ggTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCAc       <  2:1939756/101‑1 (MQ=255)
                                                                                            gTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACt      <  2:3535460/101‑1 (MQ=255)
                                                                                            gTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATAACGGTCTCTCCTCCCGTTTCACt      >  1:3377035/1‑101 (MQ=255)
                                                                                             tttCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTg     >  1:1626360/1‑101 (MQ=255)
                                                                                             tttCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTg     <  1:5348407/101‑1 (MQ=255)
                                                                                                      |                                                                                              
CAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA...GGGTTG..GTCGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGG.CCCCAACAGGGCCAG.TTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCG  >  NC_014034/2177218‑2177404

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG