Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,177,342 +G 100% coding (35/990 nt) rbsB ← ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,177,3421.→G100.0% 1372.2 370coding (35/990 nt)rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (192/176):  ref base (1/0):  total (193/177)

Only 100 of 422 total aligned reads displayed.

ACCTTCCTTCATCTTGACGAAAA..GGGTTG..GTCGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCC.G.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGG.CCCCAACAGGGCCAG.TTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGA  >  NC_014034/2177248‑2177435
                                                                                                      |                                                                                               
aCCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCa                                                                                                  <  2:4020747/101‑1 (MQ=255)
aCCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCa                                                                                                  <  1:3362050/101‑1 (MQ=255)
 ccttccttCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGCGATCAGACAGGCCCCGGTTTCGGCCTA.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.g                                                                                                <  2:7692114/101‑1 (MQ=255)
    tccttcATCTTGGCGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggc                                                                                             <  2:7168831/101‑3 (MQ=255)
    tccttcATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggc                                                                                             >  1:6106931/1‑99 (MQ=255)
    tccttcATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggc                                                                                             <  1:2908007/101‑3 (MQ=255)
    tccgtcATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggc                                                                                             <  1:7525396/101‑3 (MQ=255)
      cttcATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcag                                                                                           >  1:6270003/1‑97 (MQ=255)
      cttcATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcag                                                                                           >  1:3077793/1‑97 (MQ=255)
       ttcATCTTGACGAAAAGGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaga                                                                                          >  2:3623132/1‑96 (MQ=255)
       ttcATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaga                                                                                          <  2:7458737/101‑6 (MQ=255)
       ttcATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaga                                                                                          >  1:6386594/1‑96 (MQ=255)
        tcATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCagag                                                                                          <  2:2980233/101‑1 (MQ=255)
        tcATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCagag                                                                                          >  1:5101151/1‑101 (MQ=255)
          aTCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.c                                                                                       <  1:2816045/101‑1 (MQ=255)
           tCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.cc                                                                                      <  2:1778046/101‑1 (MQ=255)
           tCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.cc                                                                                      <  1:5224667/101‑1 (MQ=255)
           tCTTGACGAAAAAGGGGTTGCTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.cc                                                                                      <  1:5624805/101‑1 (MQ=255)
            cTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGGGCGGAAAACGCCA.gggcagacgacc                                                                                     >  2:4252056/1‑91 (MQ=255)
                        ggggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCa                                                                          <  1:1958300/92‑1 (MQ=255)
                        ggggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCa                                                                          <  2:6365038/92‑1 (MQ=255)
                         gggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAg                                                                         <  2:5449467/93‑1 (MQ=255)
                           gttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tt                                                                      <  1:9140096/95‑1 (MQ=255)
                           gttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tt                                                                      >  1:7809871/7‑101 (MQ=255)
                           gttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tt                                                                      >  1:2776335/7‑101 (MQ=255)
                             tggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttg                                                                    >  1:950038/5‑99 (MQ=255)
                             tggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttg                                                                    <  1:1036875/97‑3 (MQ=255)
                              ggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgc                                                                   >  2:1237372/4‑98 (MQ=255)
                              ggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgc                                                                   >  2:5575066/4‑98 (MQ=255)
                               gtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgcg                                                                  <  1:5944110/99‑5 (MQ=255)
                                tgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgcgc                                                                 >  1:4944395/2‑96 (MQ=255)
                                tgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgcgc                                                                 >  1:430691/2‑96 (MQ=255)
                                 gtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGca                                                                 >  2:2737188/1‑101 (MQ=255)
                                 gtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGca                                                                 >  1:895912/1‑101 (MQ=255)
                                  tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGcat                                                                <  2:6584708/101‑1 (MQ=255)
                                   cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGcatc                                                               >  2:9015895/1‑101 (MQ=255)
                                   cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGcatc                                                               >  2:5159698/1‑101 (MQ=255)
                                   cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGcatc                                                               <  2:464403/101‑1 (MQ=255)
                                      tctgggtgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCatcatc                                                            <  1:5341978/91‑1 (MQ=255)
                                       cttggtgcACAGACAGCCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCAtcatca                                                           <  2:1277318/97‑1 (MQ=255)
                                       cttggtgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCAtcatca                                                           >  2:8298669/10‑101 (MQ=255)
                                       cttggtgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCAtcatca                                                           >  1:1303239/10‑101 (MQ=255)
                                       cttggtgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCAtcatca                                                           <  2:6731768/92‑1 (MQ=255)
                                       cttggtgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCAtcatca                                                           <  2:1673067/92‑1 (MQ=255)
                                        ttggtgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCAc                                                          >  2:912922/9‑101 (MQ=255)
                                         tggtgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACg                                                         >  1:4015905/8‑101 (MQ=255)
                                         tggtgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACg                                                         <  2:6269763/94‑1 (MQ=255)
                                         ccgatctcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACg                                                         <  2:3769737/94‑1 (MQ=255)
                                            tgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtc                                                      >  1:8364926/5‑101 (MQ=255)
                                            tgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtc                                                      <  2:6120162/97‑1 (MQ=255)
                                              atcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtctc                                                    >  2:8881086/3‑101 (MQ=255)
                                               tcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtctct                                                   <  1:8006133/100‑1 (MQ=255)
                                                cagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAGCAGGGCCAGTTTGCGCATCATCACGGtctctc                                                  >  1:4555987/1‑101 (MQ=255)
                                                 agacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTctcc                                                 <  1:6574121/101‑1 (MQ=255)
                                                   acagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTctcctc                                               >  2:7296293/1‑101 (MQ=255)
                                                    cagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCtcctcc                                              >  1:7279586/1‑101 (MQ=255)
                                                     agGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTccc                                             >  1:674610/1‑101 (MQ=255)
                                                     agGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTccc                                             >  1:8684217/1‑101 (MQ=255)
                                                      ggCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCg                                            <  2:3179880/101‑1 (MQ=255)
                                                      ggCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCg                                            >  1:320988/1‑101 (MQ=255)
                                                         cccGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGttt                                         >  2:3507308/1‑101 (MQ=255)
                                                         cccGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGttt                                         >  1:7181673/1‑101 (MQ=255)
                                                          ccGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTc                                        <  2:7531611/101‑1 (MQ=255)
                                                          ccGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTc                                        <  2:1502798/101‑1 (MQ=255)
                                                          ccGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTc                                        >  2:781393/1‑101 (MQ=255)
                                                           cGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCa                                       <  1:6485027/101‑1 (MQ=255)
                                                           cGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCa                                       <  2:5344458/101‑1 (MQ=255)
                                                           cGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCa                                       >  1:3617232/1‑101 (MQ=255)
                                                           cGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCa                                       >  1:3396326/1‑101 (MQ=255)
                                                            ggTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCAc                                      >  2:6994783/1‑101 (MQ=255)
                                                            ggTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCAc                                      >  1:7395542/1‑101 (MQ=255)
                                                            ggTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCAc                                      >  1:7083506/1‑101 (MQ=255)
                                                             gTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACt                                     <  1:1452291/101‑1 (MQ=255)
                                                               ttCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGt                                   <  2:6859567/101‑1 (MQ=255)
                                                                tCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTc                                  <  1:4816551/101‑1 (MQ=255)
                                                                 cGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCACAACGGTCTCTCCTCCCGTTTCACTGTCg                                 >  1:2305421/1‑101 (MQ=255)
                                                                    gccgaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGc                               >  2:6468296/6‑101 (MQ=255)
                                                                     ccgaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcc                              <  2:2340834/97‑1 (MQ=255)
                                                                      cgaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGccc                             >  2:8591609/4‑101 (MQ=255)
                                                                       gaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCGCGGTCTCTCCTCCCGTTTCACTGTCGGcccc                            >  1:8163022/3‑101 (MQ=255)
                                                                       gaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcccc                            >  2:3356737/3‑101 (MQ=255)
                                                                       gaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcccc                            >  2:4763622/3‑101 (MQ=255)
                                                                        aaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCcct                           <  2:5664878/100‑1 (MQ=255)
                                                                         aGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCcctc                          >  1:2568378/1‑101 (MQ=255)
                                                                          gccgccCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCcctcc                         <  2:6929551/101‑1 (MQ=255)
                                                                          gccgccCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCcctcc                         <  1:152562/101‑1 (MQ=255)
                                                                           ccgccCCGGCCAGCGCGTAACACGCCAGGGCAGAGGCCCCCACCAGGGCTAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCcctcct                        <  2:8525353/101‑1 (MQ=255)
                                                                            cgccCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCctcctc                       <  2:3994066/101‑1 (MQ=255)
                                                                                ccGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCggg                   >  1:2258790/1‑101 (MQ=255)
                                                                                ccGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCggg                   >  2:7879765/1‑101 (MQ=255)
                                                                                   gCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCt                >  1:3838914/1‑101 (MQ=255)
                                                                                    ccAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTc               <  1:4193210/101‑1 (MQ=255)
                                                                                    ccAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTc               <  1:2481452/101‑1 (MQ=255)
                                                                                      aGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCgg             <  2:2013899/101‑1 (MQ=255)
                                                                                       gcgcGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGa            <  2:4446766/101‑1 (MQ=255)
                                                                                          cgGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACaa         <  1:1770953/101‑1 (MQ=255)
                                                                                           ggAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACaaa        <  2:5812124/101‑1 (MQ=255)
                                                                                            gAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAg       <  1:4763772/101‑1 (MQ=255)
                                                                                             aaaaCGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGt      >  2:4572704/1‑101 (MQ=255)
                                                                                                aCGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTg   <  2:8122528/101‑1 (MQ=255)
                                                                                                      |                                                                                               
ACCTTCCTTCATCTTGACGAAAA..GGGTTG..GTCGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCC.G.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGG.CCCCAACAGGGCCAG.TTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGA  >  NC_014034/2177248‑2177435

Base quality scores:  ATCG  < 3 ≤  ATCG  < 25 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG