Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,177,350 +C 100% coding (27/990 nt) rbsB ← ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,177,3501.→C100.0% 1425.8 383coding (27/990 nt)rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (192/190):  ref base (0/1):  total (192/191)

Only 100 of 415 total aligned reads displayed.

CATCTTGACGAAAA..GGGTTG..GTCGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCC.GGCCAGCGCGGAAAACGCCA.GG.CAGAGG.CCCCAACAGGGCCAG.TTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCG  >  NC_014034/2177257‑2177444
                                                                                                       |                                                                                               
cATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAgg                                                                                                  <  2:8085820/101‑1 (MQ=255)
 aTCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGG.c                                                                                                >  2:7874980/1‑101 (MQ=255)
 aTCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGG.c                                                                                                <  1:2816045/101‑1 (MQ=255)
  tCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGG.cc                                                                                               >  2:6317095/1‑101 (MQ=255)
   cTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGG.ccc                                                                                              >  2:5708748/1‑101 (MQ=255)
   cTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGG.ccc                                                                                              >  2:3314162/1‑101 (MQ=255)
    ttGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGG.cccc                                                                                             >  1:401383/1‑101 (MQ=255)
               ggggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCa                                                                                   <  1:1958300/92‑1 (MQ=255)
                gggttggtgtcggtcTTGGTGATCAGACAGACCCCGGTTTCGGCCGAAGCTGCCCC.GGCCAGCGCGGGAAACGCCAGGG.AAGAGGCCCCCAACACGGCCAg                                                                                  >  2:1742340/9‑101 (MQ=255)
                atcttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAg                                                                                  <  1:456571/93‑1 (MQ=255)
                 ggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAG.t                                                                                <  2:6397343/94‑1 (MQ=255)
                 ggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAG.t                                                                                >  2:8424355/8‑101 (MQ=255)
                 ggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAG.t                                                                                <  2:3995191/94‑1 (MQ=255)
                  gttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGCAAACGCCAGGG.CAGAGGCCCCCAACCGGGCCCG.tt                                                                               >  1:2529788/7‑101 (MQ=255)
                  gttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAG.tt                                                                               >  1:2776335/7‑101 (MQ=255)
                  gttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAG.tt                                                                               >  1:7809871/7‑101 (MQ=255)
                   ttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAG.ttt                                                                              >  2:3769166/6‑100 (MQ=255)
                   ttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAG.ttt                                                                              >  2:7806514/6‑100 (MQ=255)
                   ttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAG.ttt                                                                              <  2:1031674/96‑2 (MQ=255)
                    tggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAG.tttg                                                                             >  2:3880808/5‑99 (MQ=255)
                     ggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAG.tttgc                                                                            >  2:1237372/4‑98 (MQ=255)
                     ggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAG.tttgc                                                                            <  1:8972545/98‑4 (MQ=255)
                        gtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGca                                                                          >  2:2737188/1‑101 (MQ=255)
                        gtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGca                                                                          >  2:8582760/1‑101 (MQ=255)
                         tcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGcat                                                                         >  1:3518739/1‑101 (MQ=255)
                          cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGcatc                                                                        >  2:3395883/1‑101 (MQ=255)
                          cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGcatc                                                                        <  2:464403/101‑1 (MQ=255)
                          cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGcatc                                                                        >  2:9015895/1‑101 (MQ=255)
                              cttggtgcACAGACAGCCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCAtcatca                                                                    <  2:1277318/97‑1 (MQ=255)
                              cttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCAtcatca                                                                    >  2:4332174/10‑101 (MQ=255)
                              cttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCAtcatca                                                                    >  1:914632/10‑101 (MQ=255)
                              ctgggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCAtcatca                                                                    <  1:3395987/92‑1 (MQ=255)
                               ttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCAc                                                                   >  1:6999910/9‑101 (MQ=255)
                               ttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCAc                                                                   <  2:6041224/93‑1 (MQ=255)
                               ttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCAc                                                                   >  2:912922/9‑101 (MQ=255)
                               tgggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCAc                                                                   <  2:2238598/93‑1 (MQ=255)
                                tggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACg                                                                  <  1:62672/94‑1 (MQ=255)
                                 ggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACgg                                                                 <  2:1422047/95‑1 (MQ=255)
                                 ggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACgg                                                                 >  2:6966105/7‑101 (MQ=255)
                                   tgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtc                                                               >  1:5284052/5‑101 (MQ=255)
                                   tgagcagacagGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtc                                                               <  1:7348207/97‑1 (MQ=255)
                                    gatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtct                                                              <  1:3274474/98‑1 (MQ=255)
                                    gatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtct                                                              <  1:1263846/98‑1 (MQ=255)
                                    gatcagacagCCCCCGGTTTCGGCCGAAACCGCCCC.CGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtct                                                              >  2:302045/4‑101 (MQ=255)
                                     atcagacagGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtctc                                                             >  2:7181286/3‑101 (MQ=255)
                                     atcagacagGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtctc                                                             >  2:8881086/3‑101 (MQ=255)
                                       cagacagGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtctctc                                                           >  1:4295405/1‑101 (MQ=255)
                                        agacagGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTctcc                                                          >  2:738681/1‑101 (MQ=255)
                                        agacagGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTctcc                                                          <  1:6574121/101‑1 (MQ=255)
                                        agacagGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCA.GGCCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGCCTctcc                                                          >  2:1619022/1‑101 (MQ=255)
                                          acagGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTctcctc                                                        >  2:4085493/1‑101 (MQ=255)
                                           agggCCCGGGTTCCGCCCG.AGCCGCCCCGGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCtcctcc                                                       <  1:4163071/99‑1 (MQ=255)
                                            agGCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTccc                                                      <  2:5144387/101‑1 (MQ=255)
                                             ggCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCg                                                     >  1:8509897/1‑101 (MQ=255)
                                              gCCCCGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGt                                                    >  2:752818/1‑101 (MQ=255)
                                                cccGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGttt                                                  <  1:7163391/101‑1 (MQ=255)
                                                  cGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCa                                                >  1:1784954/1‑101 (MQ=255)
                                                  cGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCa                                                <  1:6485027/101‑1 (MQ=255)
                                                  cGGTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCa                                                <  2:4901793/101‑1 (MQ=255)
                                                   ggTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCAc                                               <  2:1939756/101‑1 (MQ=255)
                                                    gTTTCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACt                                              <  2:3535460/101‑1 (MQ=255)
                                                     tttCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTg                                             <  1:5348407/101‑1 (MQ=255)
                                                     tttCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTg                                             >  1:7271907/1‑101 (MQ=255)
                                                       tCGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTc                                           <  1:4816551/101‑1 (MQ=255)
                                                        cGGCCGAAGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCg                                          >  2:6214861/1‑101 (MQ=255)
                                                             gaaGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcccc                                     >  1:5083194/3‑101 (MQ=255)
                                                              aaGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCcct                                    >  2:7688345/2‑101 (MQ=255)
                                                               aGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTACACTGTCGGCCcctc                                   >  1:804588/1‑101 (MQ=255)
                                                               aGCCGCCCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTATCCTCCCGTTTCACTGTCGGCCcctc                                   <  1:3797735/101‑1 (MQ=255)
                                                                gccgccGC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCcctcc                                  <  2:6738356/101‑1 (MQ=255)
                                                                   gccCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCtcctcc                               <  1:2357430/101‑1 (MQ=255)
                                                                   gccCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCtcctcc                               >  1:6738542/1‑101 (MQ=255)
                                                                   gccCC.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCtcctcc                               <  1:6081091/101‑1 (MQ=255)
                                                                     ccc.GGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCgg                             >  1:1489935/1‑101 (MQ=255)
                                                                         ggCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGc                          >  1:7439678/1‑101 (MQ=255)
                                                                          gCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCt                         >  1:3073834/1‑101 (MQ=255)
                                                                          gCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCt                         <  1:7936349/101‑1 (MQ=255)
                                                                          gCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCt                         <  2:649720/101‑1 (MQ=255)
                                                                           ccAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTc                        <  2:5326017/101‑1 (MQ=255)
                                                                           ccAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTc                        >  2:5605600/1‑101 (MQ=255)
                                                                           ccAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTc                        >  1:4913078/1‑101 (MQ=255)
                                                                             aGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCACgg                      <  1:8810659/101‑1 (MQ=255)
                                                                                 cgGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCGTCATCACGGTCTCTCCTCCCGTTTCACTATCGGCCCCTCCTCCGGGGCTCGGACaa                  >  1:2261617/1‑101 (MQ=255)
                                                                                 cgGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACaa                  <  1:8346618/101‑1 (MQ=255)
                                                                                  ggAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACaaa                 >  1:6386527/1‑101 (MQ=255)
                                                                                   gAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAg                <  1:4763772/101‑1 (MQ=255)
                                                                                    aaaaCGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGt               >  2:8641095/1‑101 (MQ=255)
                                                                                    aaaaCGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGt               >  2:4572704/1‑101 (MQ=255)
                                                                                     aaaCGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGCCAAAGTc              >  2:6871036/1‑101 (MQ=255)
                                                                                      aaCGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCt             >  1:5639407/1‑101 (MQ=255)
                                                                                         gccaggg.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGAc          >  1:1398918/6‑101 (MQ=255)
                                                                                          ccaggg.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGAcc         <  1:1036604/97‑1 (MQ=255)
                                                                                           caggg.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGAccc        <  2:8249047/98‑1 (MQ=255)
                                                                                            aggg.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGCCAAAGTCTGACCCg       >  2:3207983/3‑101 (MQ=255)
                                                                                            aggg.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCg       <  2:2456508/99‑1 (MQ=255)
                                                                                            aggg.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCg       >  2:5528723/3‑101 (MQ=255)
                                                                                              gg.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAg     >  2:4508463/1‑101 (MQ=255)
                                                                                                 cAGAGGCCCCCAACATGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGCCTGACCCGAGTc   <  2:6697434/101‑1 (MQ=255)
                                                                                                 cAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTc   >  1:2908600/1‑101 (MQ=255)
                                                                                                  agagGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCg  >  1:1655093/1‑101 (MQ=255)
                                                                                                       |                                                                                               
CATCTTGACGAAAA..GGGTTG..GTCGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCC.GGCCAGCGCGGAAAACGCCA.GG.CAGAGG.CCCCAACAGGGCCAG.TTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCG  >  NC_014034/2177257‑2177444

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG