Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,180,161 +C 100% coding (850/1314 nt) RCAP_rcc02022 → polyols ABC transporter periplasmic polyols‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,180,1611.→C100.0% 1310.3 350coding (850/1314 nt)RCAP_rcc02022polyols ABC transporter periplasmic polyols‑binding protein
Reads supporting (aligned to +/- strand):  new base (178/170):  ref base (0/0):  total (180/170)

Only 100 of 395 total aligned reads displayed.

CTTCCAGCAGGGCCATTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCG.GCTGGC.CCGAACAAG.G.C.GGTGTCCGCAAGCATGGCAACTGGCTCTGGGCCTGGAACCCTCGCCAATTCCGGCGGGCACGCAAAAAGGCCGAGGCG  >  NC_014034/2180061‑2180256
                                                                                                     |                                                                                                   
ctTCCAGCAGGGCCATTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGtcggcttcg                                                                                                      >  1:207406/1‑101 (MQ=255)
ctTCCAGCAGGGCCATTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGtcggcttcg                                                                                                      <  2:3833048/101‑1 (MQ=255)
ctTCCAGCAGGGCCATTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGtcggcttcg                                                                                                      <  2:1920235/101‑1 (MQ=255)
 ttCCAGCAGGGCCATTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGtcggcttcgc                                                                                                     >  1:1568397/1‑100 (MQ=255)
 ttCCAGCAGGGCCATTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGtcggcttcgc                                                                                                     <  2:230658/101‑2 (MQ=255)
   ccAGCAGGGCCATTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGtcggcttcgcgc                                                                                                   >  2:2810586/1‑98 (MQ=255)
        agGGCCATTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGcg                                                                                              >  2:5779023/1‑100 (MQ=255)
        agGGCCATTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGcg                                                                                              >  1:5903655/1‑100 (MQ=255)
          ggCCATTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGtcggcttcg.gcccggctgc                                                                                           >  2:8965406/1‑93 (MQ=255)
          ggCCATTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGcgcc                                                                                            <  2:6143971/101‑4 (MQ=255)
              aTTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAc                                                                                        >  1:3889175/1‑101 (MQ=255)
              aTTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAc                                                                                        >  1:8655232/1‑101 (MQ=255)
                tccggctgggggATCGATGCGGCCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACaa                                                                                      <  2:4227175/91‑1 (MQ=255)
                tGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACaa                                                                                      <  1:3062394/101‑1 (MQ=255)
                   ggCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.G.c                                                                                 >  1:6737616/1‑101 (MQ=255)
                   ggCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.G.c                                                                                 >  1:1486458/1‑101 (MQ=255)
                   ggCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.G.c                                                                                 >  2:5886312/1‑101 (MQ=255)
                    gCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAgaga                                                                                  >  2:8026116/1‑98 (MQ=255)
                        gtgGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.G.ccggtg                                                                            >  1:8741604/1‑96 (MQ=255)
                         tgGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.Ggtgt                                                                           <  1:1929181/101‑1 (MQ=255)
                         tgGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCAGCTTCGCGCTGGCGCCGAACAAG.GCC.Ggtgt                                                                           <  2:5406516/101‑1 (MQ=255)
                          ggATCGATGCGACCGTGGCCTCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCTAACAAG.GCC.GGTGTc                                                                          <  1:7251588/101‑1 (MQ=255)
                          ggATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTc                                                                          >  1:8309557/1‑101 (MQ=255)
                          ggATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTc                                                                          <  1:7453451/101‑1 (MQ=255)
                          ggATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGAGCTGGCGCCGAACAAG.G.ccggggtc                                                                          >  2:7085949/1‑94 (MQ=255)
                          ggATCGATCCGCCCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTc                                                                          <  2:7351753/101‑1 (MQ=255)
                           gATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTcg                                                                         <  2:3425846/101‑2 (MQ=255)
                             tCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTcgcc                                                                       <  2:4038151/101‑4 (MQ=255)
                              cGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTcgcca                                                                      <  1:4573075/101‑5 (MQ=255)
                              cGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTcgcca                                                                      <  2:2069894/101‑5 (MQ=255)
                              cGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTcgcca                                                                      >  2:1735981/1‑97 (MQ=255)
                               gATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTcgccca                                                                     >  2:496574/1‑96 (MQ=255)
                                aTGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAg                                                                    <  2:8755076/101‑1 (MQ=255)
                                aTGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAg                                                                    >  2:161033/1‑101 (MQ=255)
                                 tGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGc                                                                   >  1:7098295/1‑101 (MQ=255)
                                      ccGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGCGTCGCCAAGCATGGc                                                              >  1:9020521/1‑101 (MQ=255)
                                       cGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCTCCAAGCATGGCa                                                             <  1:8180884/101‑1 (MQ=255)
                                       cGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCa                                                             <  1:8040220/101‑1 (MQ=255)
                                       cGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCa                                                             >  2:4633242/1‑101 (MQ=255)
                                       cGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCa                                                             <  1:3513178/101‑1 (MQ=255)
                                       cGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCa                                                             <  2:834074/101‑1 (MQ=255)
                                       cGTGGCCACCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCa                                                             <  1:2619486/101‑1 (MQ=255)
                                        gTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCaa                                                            <  1:6420623/101‑1 (MQ=255)
                                         tGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAAc                                                           >  2:2994161/1‑101 (MQ=255)
                                         tGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAAc                                                           <  1:5557146/101‑1 (MQ=255)
                                         tGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAAc                                                           >  2:5701348/1‑101 (MQ=255)
                                          ggCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAAcg                                                          >  2:859130/1‑100 (MQ=255)
                                          ggCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACt                                                          <  1:692505/101‑1 (MQ=255)
                                           gccgccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTg                                                         <  1:4956709/101‑1 (MQ=255)
                                              gccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGct                                                      <  2:10541/101‑1 (MQ=255)
                                                ctctTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGctct                                                    >  2:8864138/1‑101 (MQ=255)
                                                      cGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCt                                              >  1:2741998/1‑101 (MQ=255)
                                                       gTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTg                                             >  2:2477048/1‑101 (MQ=255)
                                                         gACCAACCCCAAGGACTCGGCCGTTGCCGATCATGGCGGCTTCGCGCTTGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTGTGGGCCTGGa                                           <  2:923400/101‑1 (MQ=255)
                                                         gACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGa                                           >  1:8450261/1‑101 (MQ=255)
                                                         gACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.G.CTGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGa                                           >  1:3720891/1‑101 (MQ=255)
                                                           ccAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAc                                         <  1:6155032/101‑1 (MQ=255)
                                                           ccAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAc                                         >  2:6944964/1‑101 (MQ=255)
                                                              aCCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcctc                                      <  2:651343/101‑3 (MQ=255)
                                                              aCCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcctc                                      <  2:8234021/101‑3 (MQ=255)
                                                                 ccAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGcc                                  >  1:1255500/1‑101 (MQ=255)
                                                                 ccAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGcc                                  <  2:1282915/101‑1 (MQ=255)
                                                                   aaGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCat                                >  1:4395526/1‑100 (MQ=255)
                                                                   aaGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCat                                >  2:3255258/1‑100 (MQ=255)
                                                                    aGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCatt                               <  2:8194507/101‑3 (MQ=255)
                                                                    aGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCatt                               <  2:4385776/101‑3 (MQ=255)
                                                                    aGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCatt                               <  1:647599/101‑3 (MQ=255)
                                                                      gACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcctagccattcc                              >  2:4498951/1‑91 (MQ=255)
                                                                      gACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattcc                             <  1:4692441/101‑5 (MQ=255)
                                                                      gACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattcc                             <  1:5341724/101‑5 (MQ=255)
                                                                      gACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattcc                             >  2:3584810/1‑97 (MQ=255)
                                                                       gcTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcg                           <  2:1427263/100‑1 (MQ=255)
                                                                       aCTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcg                           >  2:4697687/1‑101 (MQ=255)
                                                                        cTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcgg                          >  1:4133204/1‑101 (MQ=255)
                                                                           ggCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcggcgg                       >  1:3142545/1‑101 (MQ=255)
                                                                             ccGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGc                     <  1:461698/101‑1 (MQ=255)
                                                                             ccGTGACCGATAATGTCGGCTTCGCGCTGGCGCCGAACCAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGc                     <  1:7336469/101‑1 (MQ=255)
                                                                              cGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCa                    >  2:6342745/1‑101 (MQ=255)
                                                                               gTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCAc                   <  1:7890298/101‑1 (MQ=255)
                                                                               gTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCAc                   >  1:3630240/1‑101 (MQ=255)
                                                                                tGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCG.CGATTCCGGCGGGCACg                  <  1:6023080/101‑1 (MQ=255)
                                                                                tGGACGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CGTCGCC.ATTCCGGCGGGCACg                  >  1:8808132/1‑101 (MQ=255)
                                                                                 ggCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGc                 >  1:5763891/1‑101 (MQ=255)
                                                                                  gCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCa                >  1:7571742/1‑101 (MQ=255)
                                                                                  gCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCa                <  2:3364210/101‑1 (MQ=255)
                                                                                  gCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCa                >  1:6778208/1‑101 (MQ=255)
                                                                                    cGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaa              >  1:1807451/1‑101 (MQ=255)
                                                                                    cGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaa              >  1:1077218/1‑101 (MQ=255)
                                                                                    cGATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaa              <  2:6616754/101‑1 (MQ=255)
                                                                                     gATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaa             >  1:5153371/1‑101 (MQ=255)
                                                                                     gATCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaa             <  1:7679849/101‑1 (MQ=255)
                                                                                       tCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaagg           >  2:7564243/1‑99 (MQ=255)
                                                                                       tCATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaagg           >  2:2660833/1‑99 (MQ=255)
                                                                                        cATGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGCCACGCaaaaggc          >  1:9144851/1‑98 (MQ=255)
                                                                                          tGTCGGCTTCGCGCTGGCGCCGAACAAGTC.C.GGGGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCg       <  1:4242685/101‑1 (MQ=255)
                                                                                          tGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCg       >  1:2275561/1‑101 (MQ=255)
                                                                                          tGTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCg       >  2:2033585/1‑101 (MQ=255)
                                                                                           gTCGGCTTCGCGCTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCgc      >  1:2812571/1‑100 (MQ=255)
                                                                                             cggcttcgcgcTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAgg    <  2:5193521/101‑1 (MQ=255)
                                                                                              ggcttcgcgcTGGCGCCGAACAAG.GCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggc   >  2:5114613/1‑101 (MQ=255)
                                                                                                     |                                                                                                   
CTTCCAGCAGGGCCATTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCG.GCTGGC.CCGAACAAG.G.C.GGTGTCCGCAAGCATGGCAACTGGCTCTGGGCCTGGAACCCTCGCCAATTCCGGCGGGCACGCAAAAAGGCCGAGGCG  >  NC_014034/2180061‑2180256

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG