Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,185,841 +C 100% intergenic (+114/‑391) mtlK → / → RCAP_rcc02028 mannitol 2‑dehydrogenase/FeoA family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,185,8411.→C100.0% 29.4 286intergenic (+114/‑391)mtlK/RCAP_rcc02028mannitol 2‑dehydrogenase/FeoA family protein
Reads supporting (aligned to +/- strand):  new base (56/216):  ref base (12/2):  total (68/218)
Fisher's exact test strand distribution p-value = 8.81e-07
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.77e-07

Only 100 of 339 total aligned reads displayed.

CCGCCCCGGAACAGGCGGCCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.G.CC.CCGCACCCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.CCCCGAAGGCCTTGTTTTTGATGGCACAAGAAACGCCGAACCGCCCCTTTC  >  NC_014034/2185741‑2185938
                                                                                                        |                                                                                                   
ccGCCCCGGAACAGGCGGCCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.g                                                                                                      <  2:303561/101‑1 (MQ=255)
ccGCCCCGGAACAGGCGGCCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.g                                                                                                      <  2:599679/101‑1 (MQ=255)
ccGCCCCGGAACAGGCGGCCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.g                                                                                                      <  1:2640547/101‑1 (MQ=255)
   ccccGGAACAGGCGGCCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.G.cc.c                                                                                                 <  1:2890514/101‑1 (MQ=255)
    cccGGAACAGGCGGCCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.G.cc.cc                                                                                                >  1:7502340/1‑101 (MQ=255)
       ggAACAGGCGGCCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGGCTCGGCGGTGCCGAGGCGGCCCT.GCCGGGG.GTCC.G.cc.ccccc                                                                                             >  1:8757038/1‑98 (MQ=255)
       ggAACAGGCGGCCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.GGGC.G.cc.ccccc                                                                                             >  2:5837590/1‑98 (MQ=255)
       ggAACAGGCGGCCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.G.cc.cccgc                                                                                             <  2:1316728/101‑4 (MQ=255)
           cAGGCGGCCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCAccc                                                                                          >  2:3616814/1‑101 (MQ=255)
           cAGGCGGCCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCAccc                                                                                          <  2:8751181/101‑1 (MQ=255)
             ggcggcCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCAccccc                                                                                        <  1:1890990/101‑1 (MQ=255)
             ggcggcCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCAccccc                                                                                        <  2:4600430/101‑1 (MQ=255)
                ggcCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTGGCGGGGG.GGCG.G.CC.CCCCCcccccccc                                                                                     >  2:7634368/1‑99 (MQ=255)
                ggcCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.GCCC.C.CC.CCCCCCCCCCCCGc                                                                                    >  2:1044777/1‑101 (MQ=255)
                ggcCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCAcccccggc                                                                                     <  2:8451086/101‑4 (MQ=255)
                  ccctGCCCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGt                                                                                  <  1:1530718/101‑1 (MQ=25)
                   cccGCTCCCTCCCGGGCGGGGCCGCCTGAGGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTa                                                                                 <  2:3524516/101‑1 (MQ=37)
                   cccGCCCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGCTTCGCCGCGGCGGTGCCGAGGCGGCCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTa                                                                                 <  1:3741853/101‑1 (MQ=25)
                    ccGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTAt                                                                                <  2:6688422/101‑1 (MQ=255)
                    ccGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTAt                                                                                <  1:1438919/101‑1 (MQ=255)
                     cGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTAtt                                                                               <  1:1700236/101‑1 (MQ=255)
                      gCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.GGCC.C.CC.CCCCCccccccccccggcct                                                                              >  1:4466497/1‑92 (MQ=255)
                      gCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTAttt                                                                              <  2:5045566/101‑1 (MQ=255)
                      gCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTAttt                                                                              <  1:3854301/101‑1 (MQ=255)
                      gCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTAttt                                                                              <  1:2761755/101‑1 (MQ=255)
                        tccctcccGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTAt                                                                            >  1:5877391/1‑101 (MQ=255)
                         ccctcccGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATc                                                                           >  2:1925662/1‑101 (MQ=255)
                            tcccGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCa                                                                        <  1:325794/101‑1 (MQ=255)
                            gcacgggcgggGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCa                                                                        <  2:2313097/98‑1 (MQ=25)
                            ccccGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCa                                                                        <  1:4980453/100‑1 (MQ=37)
                              ccGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAg                                                                      <  1:896304/101‑1 (MQ=255)
                                gggcgggGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGaa                                                                    <  1:7657064/101‑1 (MQ=255)
                                gggcgggGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGaa                                                                    <  1:6071998/101‑1 (MQ=255)
                                gggcgggGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGaa                                                                    <  2:8891196/101‑1 (MQ=255)
                                gggcgggGCCGCCTGATGTGCAAAGCGGTTTCCCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGaa                                                                    <  2:5012369/101‑1 (MQ=37)
                                 ggcgggGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGaaa                                                                   <  1:6286762/101‑1 (MQ=255)
                                 ggcgggGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGaaa                                                                   <  2:4037967/101‑1 (MQ=255)
                                 ggcgggGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGaaa                                                                   <  2:6353094/101‑1 (MQ=255)
                                   cgggGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGaaaaa                                                                 <  1:9083003/101‑1 (MQ=255)
                                   cgggGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGGATTTATCCCCAGaaaaa                                                                 >  2:8362892/1‑101 (MQ=25)
                                   cgggGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.G.CC.CCCCCACCCCCGGCGGCTTTCTCCCAAGaaaac                                                                 >  2:4129114/1‑100 (MQ=38)
                                     gggCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.GTCCGG.CC.CCGC.CCCCCCCGCGGAGTTTTCCCAAAaaaaaac                                                               >  2:1698911/1‑99 (MQ=255)
                                     gggCCGCCTGATGTGCAAAGAGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAATagc                                                               <  1:7225092/101‑1 (MQ=25)
                                        ccgccTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAagcagc                                                            <  1:1044795/101‑1 (MQ=255)
                                          gccTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.C.GC.CCCCCCCCCCCCGCCCGCTTCTCCCCAGAAAAAACATCgg                                                          >  1:1975252/1‑101 (MQ=255)
                                            cTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCt                                                        >  2:3211401/1‑101 (MQ=255)
                                             tGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCtt                                                       <  2:5983650/101‑1 (MQ=255)
                                              gATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTg                                                      <  2:5263518/101‑1 (MQ=255)
                                               aTGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.c                                                    <  2:6266412/101‑1 (MQ=255)
                                                tgtgCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.cc                                                   >  1:5595224/1‑101 (MQ=255)
                                                tgggCAAAGCGGTTCCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.cc                                                   <  2:8613603/98‑1 (MQ=37)
                                                  tgCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTT.cccc                                                 >  2:661592/1‑101 (MQ=37)
                                                   gCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.ccccc                                                >  2:4858045/1‑100 (MQ=37)
                                                    cAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCTAAGAAAAAGCAGCGGCTTG.ccccca                                               >  2:1608474/1‑99 (MQ=25)
                                                      aaGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.CGCCGCAg                                             >  2:8780057/1‑101 (MQ=25)
                                                      aaGCGGATTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCGTG.CCCCGAAg                                             <  1:7592382/101‑1 (MQ=25)
                                                         cGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.GTCC.GCCC.CCGCACCCCCC.GCGTATTTATCCCAAGAAAAAGCAGCGGCTTT.TCCCCCAGGcc                                          >  2:8063213/1‑101 (MQ=25)
                                                           gTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.CCCCGAAGGCCtt                                        <  1:8602482/101‑1 (MQ=255)
                                                            tttCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.CCCCGAAGGCCTTg                                       <  2:6283744/101‑1 (MQ=255)
                                                             ttCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.CCCCGAAGGCCTTGt                                      <  1:2374156/101‑1 (MQ=255)
                                                              tCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.CCCCGAAGGCCTTGtt                                     <  2:4727475/101‑1 (MQ=255)
                                                               cGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.GTCC.C.CC.CCCCCACCCCCCGCGTCTTTATCCCAAGGGAAAGCAGCCGCTTGTTCCCCAGGGCCTTGtt                                     >  2:7391823/1‑101 (MQ=255)
                                                               cGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.CCCCGAAGGCCTTGttt                                    <  2:7186453/101‑1 (MQ=255)
                                                               cGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.CCCCGAAGGCCTTGttt                                    <  1:4029983/101‑1 (MQ=255)
                                                                  cTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.CCCCGAAGGCCTTGttttga                                 <  1:1671789/101‑3 (MQ=37)
                                                                    cggcggTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.CCCCGAAGGCCTTGttttgatg                               <  2:5797282/101‑5 (MQ=37)
                                                                     ggcggTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.CCCCGAAGGCCTTG.TTTTGATgg                             <  1:892719/101‑1 (MQ=37)
                                                                     ggcggTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.CCCCCAACGCCTTG.TTTTGATgg                             >  1:5312410/1‑101 (MQ=25)
                                                                      gcggTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.CCCCGAAGGCCTTG.TTTTGATGGc                            <  1:6468212/101‑1 (MQ=37)
                                                                      gcggTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.CCCCGAAGGCCTTG.TTTTGATGGc                            <  1:3149765/101‑1 (MQ=37)
                                                                        ggTGCCTGGGCTGCCCT.GCGGGGG.CTCC.G.CC.CCCGCCCCCCCGGCGTATTTATCCCAAGAAAAACCAGCGGCTTG.CCCCGAAGGCCTTG.TTTTGATGGcac                          <  2:8958308/101‑1 (MQ=25)
                                                                        ggTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.CCCCGAAGGCCTTGTTTTT.ATGGcac                          >  1:5437922/1‑101 (MQ=37)
                                                                         gTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.CCCCGAAGGCCTTG.TTTTGATGGcaca                         <  1:6841069/101‑1 (MQ=37)
                                                                         gTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.CCCCGAAGGCCTTG.TTTTGATGGcaca                         <  1:8363255/101‑1 (MQ=37)
                                                                         gTGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.CCCCGAAGGCCTTG.TTTTGATGGcaca                         <  1:6202262/101‑1 (MQ=37)
                                                                          tGCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.CCCCGAAGGCCTTG.TTTTGATGGCACaa                        <  2:1969184/101‑1 (MQ=37)
                                                                          ggCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.CCCCGAAGGCCTTG.TTTTGATGGCACaa                        <  1:3398429/100‑1 (MQ=25)
                                                                           gCCGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAg                       <  1:2082181/101‑1 (MQ=37)
                                                                            ctgAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAGa                      <  1:8280973/99‑1 (MQ=25)
                                                                            cccagccGACCCT.GCGGGGGCCCCC.C.CC.CCGCA.CCCCCGGCTTATTTATCCCAAGAAAAAGCACCGGCTTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAGa                      <  1:4297419/95‑1 (MQ=21)
                                                                            ccGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAGa                      <  1:1755735/101‑1 (MQ=37)
                                                                            ccGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAGa                      <  1:5821578/101‑1 (MQ=37)
                                                                            ccGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAGa                      <  1:8072611/101‑1 (MQ=37)
                                                                            ccGAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.CCCCCAAGGCCCTGTTTTT.ATGGGCCAAGa                      >  1:4852373/1‑101 (MQ=21)
                                                                              gAGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAGaaa                    <  2:4308917/101‑1 (MQ=37)
                                                                               aGGCGACTCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAGAAAc                   <  2:7810987/101‑1 (MQ=25)
                                                                               aGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAGAAAc                   <  1:1245244/101‑1 (MQ=37)
                                                                               aGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAGAAAc                   <  2:705523/101‑1 (MQ=37)
                                                                               aGGCGACCCT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAGAAAc                   <  1:6685120/101‑1 (MQ=37)
                                                                                   gagccT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCg               <  2:4419971/98‑1 (MQ=25)
                                                                                   gACCCT.GCGGGGG.CTCC.G.CCGCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.TCGCGAAAGGCTTG.TTTTGATGGCGCAAGaaagacag               >  2:2907315/1‑96 (MQ=18)
                                                                                       cT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAAcc           <  2:7260769/101‑1 (MQ=37)
                                                                                       cT.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAAcc           <  1:464783/101‑1 (MQ=37)
                                                                                        t.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCg          <  1:7972348/101‑1 (MQ=37)
                                                                                        t.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCg          <  1:659274/101‑1 (MQ=37)
                                                                                        t.GCGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCg          <  2:6026185/101‑1 (MQ=37)
                                                                                           cGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGcc        <  2:2401195/101‑1 (MQ=37)
                                                                                           cGGGGG.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAGAAAAGCCGAACCGcc        >  1:4740188/1‑101 (MQ=25)
                                                                                            ggggg.CTCC.GCCC.CCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGccc       >  2:6808705/1‑101 (MQ=37)
                                                                                                  cTCC.G.CC.CCCGCCCCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.CCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCcttt  <  1:6876111/101‑2 (MQ=38)
                                                                                                        |                                                                                                   
CCGCCCCGGAACAGGCGGCCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCT.GCGGGGG.CTCC.G.CC.CCGCACCCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTG.CCCCGAAGGCCTTGTTTTTGATGGCACAAGAAACGCCGAACCGCCCCTTTC  >  NC_014034/2185741‑2185938

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG