Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,185,930 +C 91.2% intergenic (+203/‑302) mtlK → / → RCAP_rcc02028 mannitol 2‑dehydrogenase/FeoA family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,185,9301.→C91.2% 53.2 ‑3.0 ‑1.4215intergenic (+203/‑302)mtlK/RCAP_rcc02028mannitol 2‑dehydrogenase/FeoA family protein
Reads supporting (aligned to +/- strand):  new base (89/107):  ref base (4/15):  total (93/122)
Fisher's exact test strand distribution p-value = 5.19e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.89e-02

Only 100 of 243 total aligned reads displayed.

TGCGGGGGCTCCG.CCCCGCACCCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGTTTTTGATGGCACAAGAAACGCCGAACCG.CC.CCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..G..CCCGCCGGGTCTCCTCCGGGAGCAAGGCCGAGGCGAGACACCCCAGCGC  >  NC_014034/2185829‑2186024
                                                                                                       |                                                                                                   
tGCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCg                                                                                                      <  1:659274/101‑1 (MQ=37)
 gCGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCG.c                                                                                                    <  2:1662913/101‑1 (MQ=37)
  cGGGGGCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCG.cc                                                                                                   <  2:360572/101‑1 (MQ=37)
    ggggCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCG.cc.cc                                                                                                <  2:3745344/101‑1 (MQ=37)
     gggCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCG.cc.ccc                                                                                               <  2:6124812/101‑2 (MQ=37)
     gggCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCG.cc.ccc                                                                                               <  2:1166079/101‑2 (MQ=37)
      ggCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCG.cc.ccct                                                                                              <  1:2641347/101‑3 (MQ=37)
      ggCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCG.cc.ccct                                                                                              <  1:8022420/101‑3 (MQ=37)
       gCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCG.CC.CCctt                                                                                             <  1:602248/101‑1 (MQ=37)
       gCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCG.CC.CCctt                                                                                             <  1:1449788/101‑1 (MQ=37)
       gCTCCGCCCCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCG.CC.CCctt                                                                                             <  2:454156/101‑1 (MQ=37)
        cTCCG.CCCCCGCCCCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCG.CC.CCcttt                                                                                            <  1:6876111/101‑2 (MQ=38)
         tccgcccccGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCG.CC.CCctttc                                                                                           >  1:6479435/6‑99 (MQ=37)
         tccgcccccGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCG.CC.CCctttc                                                                                           <  2:6995916/96‑3 (MQ=37)
         tccgcccccGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCG.CC.CCctttc                                                                                           <  1:3284470/96‑3 (MQ=37)
          ccgcccccGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCa                                                                                           <  2:4962230/97‑1 (MQ=37)
          ccgcccccGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCa                                                                                           <  1:3239008/97‑1 (MQ=37)
          ccgcccccGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCa                                                                                           <  2:6471484/97‑1 (MQ=37)
          ccgcccccGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCa                                                                                           <  1:3897967/97‑1 (MQ=37)
           cgcccccGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCAc                                                                                          >  1:5097612/4‑101 (MQ=38)
            gcccccGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCAcc                                                                                         <  2:7668689/99‑1 (MQ=38)
              ccccGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCtt                                                                                       <  1:2880664/101‑1 (MQ=38)
              ccccGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCtt                                                                                       <  2:6587612/101‑1 (MQ=38)
              ccccGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCtt                                                                                       >  2:1349073/1‑101 (MQ=255)
                   gcacccccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGcc                                                                                   >  1:744615/4‑100 (MQ=255)
                   gcacccccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCt                                                                                   <  1:8664902/98‑1 (MQ=38)
                    cacccccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTc                                                                                  <  2:6503009/99‑1 (MQ=39)
                    cacccccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTc                                                                                  <  1:7710678/99‑1 (MQ=39)
                    cacccccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTc                                                                                  <  1:2822191/99‑1 (MQ=39)
                      cccccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTcca                                                                                >  2:7814156/1‑99 (MQ=255)
                        cccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAat                                                                              <  1:1068736/101‑1 (MQ=255)
                        cccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAat                                                                              <  2:4797005/101‑1 (MQ=255)
                        cccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAat                                                                              <  2:2001929/101‑1 (MQ=255)
                         ccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAata                                                                             <  1:8378819/101‑1 (MQ=255)
                         ccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAata                                                                             <  2:3845334/101‑1 (MQ=255)
                          cGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAatat                                                                            >  2:807621/1‑101 (MQ=255)
                          cGGCGTATTTATCCCAAGAAAAAGCAGCGGCCTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAatat                                                                            <  1:5870042/101‑1 (MQ=255)
                           ggCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATc                                                                           <  2:2686862/101‑1 (MQ=255)
                           ggCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATc                                                                           >  1:9057702/1‑101 (MQ=255)
                           ggCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATc                                                                           >  1:358856/1‑101 (MQ=255)
                            gCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATcc                                                                          <  2:8227880/101‑1 (MQ=255)
                            gCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATcc                                                                          <  1:4684685/101‑1 (MQ=255)
                             cGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATccc                                                                         <  1:7616185/101‑1 (MQ=255)
                             cGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATccc                                                                         <  2:5982507/101‑1 (MQ=255)
                             cGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATccc                                                                         <  1:7916808/101‑1 (MQ=255)
                              gTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATCCCg                                                                        >  2:8669087/1‑101 (MQ=255)
                              gTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATCCCg                                                                        <  1:7013288/101‑1 (MQ=255)
                              gTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATCCCg                                                                        >  1:5698664/1‑101 (MQ=255)
                               tATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATCCCgg                                                                       >  1:1967462/1‑101 (MQ=255)
                                aTTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTTGCTCAAATATCCCggg                                                                      >  2:9001181/1‑101 (MQ=255)
                                aTTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATCCCggg                                                                      >  1:7834302/1‑101 (MQ=255)
                                aTTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATCCCggg                                                                      >  1:7497912/1‑101 (MQ=255)
                                aTTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATCCCggg                                                                      >  2:8385570/1‑101 (MQ=255)
                                aTTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATCCCggg                                                                      >  2:2773573/1‑101 (MQ=255)
                                 tttATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTTGCTCAAATATcccccgg                                                                     >  1:4054532/1‑97 (MQ=255)
                                 tttATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATCCCgggg                                                                     >  1:4496365/1‑101 (MQ=255)
                                 tttATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATCCCgggg                                                                     >  1:6178934/1‑101 (MQ=255)
                                 tttATCCCAAGAAAAAGCAGCGGCTTGACCCGAAGGCCTTG.TTTTGATGGAACAAGAAACGCCGAACCG.CCACCTTTCACCTTGGCTCAAATATCCCgggg                                                                     >  1:2541006/1‑101 (MQ=255)
                                   tATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATCCCcggggg                                                                   >  1:1842727/1‑100 (MQ=255)
                                   tATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATCCCGGGGGt                                                                   >  1:5545492/1‑101 (MQ=255)
                                    aTCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATCCCggggggc                                                                  >  2:1116298/1‑99 (MQ=38)
                                    aTCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATCCCGGGGGTc                                                                  <  2:1878874/101‑1 (MQ=255)
                                    aTCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATCCCCGGGGTc                                                                  >  1:4165266/1‑101 (MQ=255)
                                    aTCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCCTGGCTCAAATATCCCcgggggc                                                                  >  2:3129412/1‑99 (MQ=255)
                                     tCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATCCCGGGGGTcc                                                                 >  2:6740384/1‑101 (MQ=255)
                                     tCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATCCCGGGGGTcc                                                                 <  2:2186846/101‑1 (MQ=255)
                                     tCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATCCCGGGGGTcc                                                                 <  2:2328803/101‑1 (MQ=255)
                                     tCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATCCCGGGGGTcc                                                                 <  1:23021/101‑1 (MQ=255)
                                     tCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATCCCGGGGGTcc                                                                 <  1:6506180/101‑1 (MQ=255)
                                     tCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATCCCGGGGGTcc                                                                 <  2:4993041/101‑1 (MQ=255)
                                     tCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATCCCGGGGGTcc                                                                 <  1:5302157/101‑1 (MQ=255)
                                      cccAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATCCCGGGGGTCCg                                                                >  1:3529884/1‑101 (MQ=255)
                                      cccAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATCCCGGGGGTCCg                                                                <  2:4435201/101‑1 (MQ=255)
                                      cccAAGAAAAAGCACCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATCCCGGGGGTCCg                                                                <  1:81896/101‑1 (MQ=39)
                                       ccAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCCCCTTGGCTCAAATATCCCGGGGGTCCgg                                                               <  2:2920080/101‑1 (MQ=255)
                                       ccAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATCCCGGGGGTCCgg                                                               <  1:2110343/101‑1 (MQ=255)
                                       ccAAGAAAAAGCAGCGGCTTGCCCCGAAAGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATCCCGGGGGTCCgg                                                               >  2:423304/1‑101 (MQ=38)
                                        cAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATCCCGGGGGTCCggg                                                              >  1:4988475/1‑101 (MQ=255)
                                         aaGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATCCCGGGGGTCCgggg                                                             <  1:3149099/101‑1 (MQ=255)
                                           gAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGc                                                           >  2:4662222/1‑101 (MQ=255)
                                           gAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGc                                                           >  1:6787536/1‑101 (MQ=255)
                                           gAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGc                                                           <  1:7359396/101‑1 (MQ=255)
                                           gAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGc                                                           >  1:2246510/1‑101 (MQ=255)
                                              aaaGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCggg                                                        >  1:519111/1‑100 (MQ=38)
                                              aaaGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGa                                                        >  1:6215721/1‑101 (MQ=255)
                                               aaGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCgggg                                                       >  1:2277715/1‑99 (MQ=38)
                                               aaGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..g                                                     >  2:882043/1‑101 (MQ=255)
                                                agcagcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATCCCGGGGGGCCGGGGGCGGA..G..c                                                  >  2:4708284/1‑101 (MQ=38)
                                                agcagcGGCTTGCCCCGAAGCCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..G..c                                                  <  1:7217022/101‑1 (MQ=37)
                                                  cagcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..G..ccc                                                >  1:8901137/1‑101 (MQ=255)
                                                   agcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..G..cccc                                               <  2:753010/101‑2 (MQ=37)
                                                    gcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..G..ccccc                                              <  1:1773115/101‑3 (MQ=37)
                                                    gcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..G..ccccc                                              <  2:6178695/101‑3 (MQ=37)
                                                    gcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..G..ccccc                                              <  2:5437737/101‑3 (MQ=37)
                                                     cGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..G..cccccg                                             <  2:472920/101‑4 (MQ=37)
                                                         ttGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCGCCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..G..cccccggcgg                                         >  2:1629877/1‑94 (MQ=25)
                                                          tGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCC.CCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..G..cccccggcggg                                        >  2:1883161/1‑93 (MQ=21)
                                                                                         gAACCGCCGACCCCCCC.CCTTTCCCCTGGGCTAAAATCTCCGGGGGGTCCGGGGGCGGACCC..CCCGGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAacac          <  1:5525199/101‑1 (MQ=0)
                                                                                            aCGCCGACCCGCCC.CCTTTCGCCTTGGCTCAAATATCCGGGGGGTCGGGGGGCGGA..GCCCCCGGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACAcccc       <  1:1116316/101‑1 (MQ=2)
                                                                                            aCGCCGACCCGCCC.CCTTTCCCCTGGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..GCCCCCGGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACAcccc       <  2:1511626/101‑1 (MQ=2)
                                                                                                       |                                                                                                   
TGCGGGGGCTCCG.CCCCGCACCCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGTTTTTGATGGCACAAGAAACGCCGAACCG.CC.CCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..G..CCCGCCGGGTCTCCTCCGGGAGCAAGGCCGAGGCGAGACACCCCAGCGC  >  NC_014034/2185829‑2186024

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG