Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,280 +C 100% intergenic (‑76/‑229) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,2801.→C100.0% 1592.0 429intergenic (‑76/‑229)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (221/205):  ref base (2/1):  total (223/206)

Only 100 of 494 total aligned reads displayed.

TCGAGGACAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAG  >  NC_014034/2249180‑2249375
                                                                                                     |                                                                                                
gcGAGGACAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTg                                                                                                   <  2:272103/100‑1 (MQ=255)
 cGAGGACAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.c                                                                                                 <  2:5413270/101‑1 (MQ=255)
 cGAGGACAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.c                                                                                                 >  2:2037154/1‑101 (MQ=255)
 cGAGGACAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.c                                                                                                 >  1:5073247/1‑101 (MQ=255)
  gAGGACAGAAGATCGCCGGCCATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.cc                                                                                                >  2:4726294/1‑101 (MQ=255)
  gAGGACAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.cc                                                                                                >  1:996670/1‑101 (MQ=255)
  gAGGACAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.cc                                                                                                >  1:1967874/1‑101 (MQ=255)
   aGGACAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.ccc                                                                                               >  1:398189/1‑101 (MQ=255)
    ggACAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.ccc.c                                                                                             >  2:3658844/1‑101 (MQ=255)
     gACAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATGCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.ccc.cc                                                                                            <  1:1575664/101‑2 (MQ=255)
      aCAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.ccc.ccg                                                                                           <  2:6049647/101‑3 (MQ=255)
      aCAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.ccc.ccg                                                                                           <  2:3349295/101‑3 (MQ=255)
       cAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.ccc.ccgt                                                                                          <  2:8448875/101‑4 (MQ=255)
        agaagaTCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.ccc.ccgtc                                                                                         >  1:1360277/1‑97 (MQ=255)
         gaagaTCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.ccc.ccgtcc                                                                                        >  2:3813589/1‑96 (MQ=255)
            gaTCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCcc.cgtcccgt                                                                                      <  1:7690127/101‑1 (MQ=255)
            gaTCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCcc.cgtcccgt                                                                                      <  1:3576622/101‑1 (MQ=255)
             aTCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTg                                                                                     >  1:2704567/1‑101 (MQ=255)
              tCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGc                                                                                    <  2:7031046/101‑1 (MQ=255)
                gCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCg                                                                                  >  2:512204/1‑101 (MQ=255)
                gCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCg                                                                                  >  2:1452841/1‑101 (MQ=255)
                  cGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCggg                                                                                >  1:3266519/1‑101 (MQ=255)
                  cGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCggg                                                                                <  2:674091/101‑1 (MQ=255)
                   ggACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGa                                                                               <  2:2330974/101‑1 (MQ=255)
                    gACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGAc                                                                              >  2:4904167/1‑101 (MQ=255)
                     aCATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACt                                                                             <  2:4406757/101‑1 (MQ=255)
                      cATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTc                                                                            >  2:8584630/1‑101 (MQ=255)
                      cATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCGCGTCCCGTGCCGGGACTc                                                                            <  2:3582368/101‑1 (MQ=255)
                       aTGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTcc                                                                           >  2:9003410/1‑101 (MQ=255)
                       aTGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTcc                                                                           >  1:8502743/1‑101 (MQ=255)
                        tGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTccc                                                                          >  1:694110/1‑101 (MQ=255)
                        tGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTccc                                                                          >  1:8139662/1‑101 (MQ=255)
                         ggTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTcccc                                                                         >  1:4827344/1‑101 (MQ=255)
                          tcttccgatctCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTccccc                                                                        <  1:2613958/91‑1 (MQ=255)
                          gTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTccccc                                                                        >  1:7522385/1‑101 (MQ=255)
                          gTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTccccc                                                                        <  1:3677169/101‑1 (MQ=255)
                           tGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCg                                                                       <  1:5704715/101‑1 (MQ=255)
                            gCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCgg                                                                      >  1:5535386/1‑101 (MQ=255)
                            gCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCgg                                                                      <  1:7916160/101‑1 (MQ=255)
                            gCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCgg                                                                      >  1:2220650/1‑101 (MQ=255)
                               cGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGat                                                                   >  2:590838/1‑101 (MQ=255)
                                   ttCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATAttt                                                               <  1:6244356/101‑1 (MQ=255)
                                    tCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTg                                                              >  2:162261/1‑101 (MQ=255)
                                     cATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGaagatcgg                                                             >  1:6797227/1‑94 (MQ=255)
                                     cATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGa                                                             >  2:767683/1‑101 (MQ=255)
                                      aTTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGGTATTTgcg                                                            >  2:2906005/1‑99 (MQ=255)
                                      aTTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAg                                                            >  1:2158305/1‑101 (MQ=255)
                                       ttGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAgg                                                           <  1:518065/101‑1 (MQ=255)
                                        tGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCATCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGc                                                          <  1:6553760/101‑1 (MQ=255)
                                        tGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAgga                                                          <  1:4201434/101‑2 (MQ=255)
                                         ggTGTTCTTGAGATGCACAGTTACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCa                                                         <  1:5643643/101‑1 (MQ=255)
                                         ggTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCa                                                         <  2:3060401/101‑1 (MQ=255)
                                           tgtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAg                                                       <  1:304108/101‑1 (MQ=255)
                                            gtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGa                                                      <  2:5180006/101‑1 (MQ=255)
                                             ttCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGAt                                                     <  2:9126043/101‑1 (MQ=255)
                                              tCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATg                                                    >  2:1520975/1‑101 (MQ=255)
                                              tCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATg                                                    >  2:2115071/1‑101 (MQ=255)
                                              tCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATg                                                    <  2:4183951/101‑1 (MQ=255)
                                               cTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGa                                                   <  2:5600325/101‑1 (MQ=255)
                                               cTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGa                                                   >  1:1807667/1‑101 (MQ=255)
                                               cTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGa                                                   <  1:8777426/101‑1 (MQ=255)
                                               cTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGa                                                   >  2:8129495/1‑101 (MQ=255)
                                                ctGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGaa                                                  <  1:5874473/100‑1 (MQ=255)
                                                 tGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAg                                                 >  2:2769049/1‑101 (MQ=255)
                                                    gaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAgggg                                              <  1:3471470/101‑1 (MQ=255)
                                                      tGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAgggggg                                            >  2:7811866/1‑101 (MQ=255)
                                                          caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCttt                                        <  2:8343677/101‑1 (MQ=255)
                                                          caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCttt                                        <  2:7925949/101‑1 (MQ=255)
                                                           aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTc                                       >  1:2398654/1‑101 (MQ=255)
                                                             aTACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCAt                                     >  2:5325167/1‑101 (MQ=255)
                                                              tACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATc                                    >  1:1735478/1‑101 (MQ=255)
                                                               aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCt                                   >  2:6328323/1‑101 (MQ=255)
                                                               aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCt                                   <  1:8321582/101‑1 (MQ=255)
                                                               aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCt                                   >  2:7358618/1‑101 (MQ=255)
                                                                 cGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGTGGCTTTCATCTTg                                 <  1:8193465/101‑1 (MQ=255)
                                                                  gCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATATGAGGCAAGATGAAGGGGGGCTTTCATCTTGc                                >  1:4383949/1‑101 (MQ=255)
                                                                    aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCg                              >  1:2085417/1‑101 (MQ=255)
                                                                      ctggggCACCCGGGGCAAGAAGGGGACTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGaa                            <  1:4646268/98‑1 (MQ=38)
                                                                      cTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGaa                            <  1:6630877/101‑1 (MQ=255)
                                                                      cTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGaa                            >  2:1165130/1‑101 (MQ=255)
                                                                       ttGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGaaa                           <  1:1091380/101‑1 (MQ=255)
                                                                       ttGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGaaa                           >  2:179719/1‑101 (MQ=255)
                                                                       ttGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGaaa                           >  1:7407795/1‑101 (MQ=255)
                                                                        tGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGaaaa                          <  1:1503672/101‑1 (MQ=255)
                                                                        tGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATATTGCCGaaaa                          <  1:7775653/101‑1 (MQ=255)
                                                                         gggCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAat                         <  1:3845200/101‑1 (MQ=255)
                                                                         gggCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAat                         <  2:6608386/101‑1 (MQ=255)
                                                                          ggCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAata                        <  2:1320189/101‑1 (MQ=255)
                                                                           gCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAatat                       <  1:9003832/101‑1 (MQ=255)
                                                                            cACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATc                      <  2:8818152/101‑1 (MQ=255)
                                                                             aCCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATcc                     <  1:6012915/101‑1 (MQ=255)
                                                                              cccGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATccc                    <  1:1004950/101‑1 (MQ=255)
                                                                               ccGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCg                   >  2:256920/1‑101 (MQ=255)
                                                                                cGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCgg                  <  2:5689575/101‑1 (MQ=255)
                                                                                cGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCgg                  <  1:8047603/101‑1 (MQ=255)
                                                                                        gaagGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGaa          >  2:7463821/1‑101 (MQ=255)
                                                                                         aagGGGGCTCTGCCCC.CGTCCCGTGCCGGGGCTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAAt         <  1:7267913/101‑1 (MQ=255)
                                                                                          agGGGGCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTGGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATg        <  1:6118228/101‑1 (MQ=255)
                                                                                             gggCTCTGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgcg     <  1:449453/101‑1 (MQ=255)
                                                                                                ctctGCCCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAg  <  1:2844391/101‑1 (MQ=255)
                                                                                                     |                                                                                                
TCGAGGACAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCC.CGTCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAG  >  NC_014034/2249180‑2249375

Base quality scores:  ATCG  < 3 ≤  ATCG  < 25 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG