Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,300 +C 1.7% intergenic (‑96/‑209) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3001.→C 1.7% 15.0 ‑1.7 ‑1.2457intergenic (‑96/‑209)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (6/2):  ref base (222/227):  total (228/229)
Fisher's exact test strand distribution p-value = 1.75e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.59e-02

Only 100 of 526 total aligned reads displayed.

ACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCCCTTCG  >  NC_014034/2249201‑2249400
                                                                                                     |                                                                                                       
aCATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACt                                                                                                          >  1:7725245/1‑101 (MQ=255)
 cATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.c                                                                                                        >  2:8584630/1‑101 (MQ=255)
  aTGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.cc                                                                                                       <  2:3599960/101‑1 (MQ=255)
     tcttccgatctCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.ccccc                                                                                                    <  1:2613958/91‑1 (MQ=255)
     gTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.ccccc                                                                                                    <  1:7291915/101‑1 (MQ=255)
      tGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCg                                                                                                   >  2:8831032/1‑101 (MQ=255)
      tGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCg                                                                                                   <  1:5704715/101‑1 (MQ=255)
       gCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCgg                                                                                                  >  2:6664274/1‑101 (MQ=255)
       gCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCgg                                                                                                  <  2:2220583/101‑1 (MQ=255)
          cGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGat                                                                                               <  2:5687068/101‑1 (MQ=255)
          cGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGat                                                                                               <  2:2721320/101‑1 (MQ=255)
           gCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGata                                                                                              <  2:1064287/101‑1 (MQ=255)
           gCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGata                                                                                              <  2:1345493/101‑1 (MQ=255)
            cTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGatat                                                                                             >  2:649106/1‑101 (MQ=255)
            cTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGatat                                                                                             >  2:6897338/1‑101 (MQ=255)
             tttCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATAtt                                                                                            <  2:5410745/101‑1 (MQ=255)
              ttCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATAttt                                                                                           >  1:8344040/1‑101 (MQ=255)
              ttCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATAttt                                                                                           <  1:2694429/101‑1 (MQ=255)
               tCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTg                                                                                          >  1:1896035/1‑101 (MQ=255)
               tCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTg                                                                                          >  1:744401/1‑101 (MQ=255)
               tCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTg                                                                                          >  1:8136225/1‑101 (MQ=255)
                cATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGa                                                                                         >  2:3352423/1‑101 (MQ=255)
                cATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGa                                                                                         >  1:2065250/1‑101 (MQ=255)
                cATTGGTGTTCTTGAGATGCACAGATACCGCAGATTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGa                                                                                         >  1:4759067/1‑101 (MQ=255)
                 aTTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAg                                                                                        >  1:2158305/1‑101 (MQ=255)
                  tgggcgtTCCTGAGATGCACAGATACCGCAGCTTGGGCAACCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAgg                                                                                       <  2:2908010/96‑1 (MQ=38)
                   tGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCATCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGc                                                                                      <  1:6553760/101‑1 (MQ=255)
                   tGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGc                                                                                      >  2:557447/1‑101 (MQ=255)
                    ggTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCa                                                                                     <  2:3060401/101‑1 (MQ=255)
                     gtgtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCaa                                                                                    >  1:3390233/1‑101 (MQ=255)
                     gtgtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCaa                                                                                    >  1:2864983/1‑101 (MQ=255)
                          cTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGa                                                                               <  1:8777426/101‑1 (MQ=255)
                           ttGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGaa                                                                              >  1:225537/1‑101 (MQ=255)
                            tGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...g                                                                          <  2:1233247/101‑1 (MQ=255)
                             gagaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGaaag                                                                            >  2:5874238/1‑99 (MQ=255)
                               gaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gggg                                                                       >  1:7079053/1‑101 (MQ=255)
                                aTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACG.CCCCCGGGATATTTGAGGCAAGATGAA...ggggg                                                                      >  1:4668825/1‑101 (MQ=255)
                                 tGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gggggg                                                                     >  2:7811866/1‑101 (MQ=255)
                                  gCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGc                                                                    <  2:2385718/101‑1 (MQ=255)
                                   cacaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCt                                                                   <  1:6820176/101‑1 (MQ=255)
                                    acaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCtt                                                                  <  2:5080921/101‑1 (MQ=255)
                                     caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCttt                                                                 <  2:8343677/101‑1 (MQ=255)
                                      aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTc                                                                <  2:1571314/101‑1 (MQ=255)
                                      aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTc                                                                >  2:9144745/1‑101 (MQ=255)
                                        aTACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCAt                                                              <  1:1595902/101‑1 (MQ=255)
                                          aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCt                                                            <  1:4907456/101‑1 (MQ=255)
                                            cGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATCTTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTg                                                          <  1:6634893/101‑1 (MQ=255)
                                             gCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATATGAGGCAAGATGAA...GGGGGGCTTTCATCTTGc                                                         >  1:4383949/1‑101 (MQ=255)
                                                  ttGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGaaa                                                    <  1:3117329/101‑1 (MQ=255)
                                                   tGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGaaaa                                                   <  2:4654239/101‑1 (MQ=255)
                                                   tGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGaaaa                                                   <  2:3945333/101‑1 (MQ=255)
                                                    gggCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAat                                                  <  1:3845200/101‑1 (MQ=255)
                                                       cACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATc                                               <  1:7930419/101‑1 (MQ=255)
                                                       cACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATc                                               <  1:1682908/101‑1 (MQ=255)
                                                        aCCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATcc                                              >  1:7564228/1‑101 (MQ=255)
                                                        aCCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATcc                                              <  1:6012915/101‑1 (MQ=255)
                                                         cccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATccc                                             <  1:1004950/101‑1 (MQ=255)
                                                         cccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATccc                                             <  1:512212/101‑1 (MQ=255)
                                                          ccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCg                                            >  2:256920/1‑101 (MQ=255)
                                                           cGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCgg                                           >  2:9101089/1‑101 (MQ=255)
                                                            ggggCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCggg                                          >  2:7855067/1‑101 (MQ=255)
                                                                cAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCggggggg                                      >  1:1484193/1‑100 (MQ=255)
                                                                cAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCggggggg                                      >  2:1465623/1‑100 (MQ=255)
                                                                  agaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGa                                    >  1:8191813/1‑101 (MQ=255)
                                                                   gaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGaa                                   >  2:7463821/1‑101 (MQ=255)
                                                                   gaagGGGGCTCTGCCCCCGTCCCGTGCAGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGaa                                   <  1:5378764/101‑1 (MQ=255)
                                                                    aagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAAt                                  >  2:7633092/1‑101 (MQ=255)
                                                                     agGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTGGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATg                                 <  1:6118228/101‑1 (MQ=255)
                                                                        gggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgcg                              >  2:2056972/1‑101 (MQ=255)
                                                                        gggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAATGgcg                              >  1:6260475/1‑101 (MQ=255)
                                                                         ggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgcgc                             <  1:4513281/101‑1 (MQ=255)
                                                                         ggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GAGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAATAgcgc                             >  2:4211706/1‑101 (MQ=37)
                                                                         ggCTCTGCCCCCGTCCCGCGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgcgc                             <  1:2973/101‑1 (MQ=255)
                                                                             ctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGcc                         >  1:7867319/5‑101 (MQ=255)
                                                                             ctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGcc                         <  2:6044108/97‑1 (MQ=255)
                                                                               gcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCag                       >  1:5875644/3‑101 (MQ=255)
                                                                               gcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCag                       >  1:5626813/3‑101 (MQ=255)
                                                                                ccccgGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCG.AAATATCCCGGGGGGTGAATGGCGCAGCCagag                     <  1:3658952/100‑1 (MQ=255)
                                                                                   ccgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAggg                   <  1:3531674/101‑1 (MQ=255)
                                                                                      tcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAgggggg                <  1:4053185/101‑1 (MQ=255)
                                                                                       ctcgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGc               >  1:1793310/3‑101 (MQ=255)
                                                                                       cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGc               >  1:1388815/1‑101 (MQ=255)
                                                                                        ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCa              >  2:4830897/1‑101 (MQ=255)
                                                                                        ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCa              <  2:1900228/101‑1 (MQ=255)
                                                                                         cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAg             >  2:8447664/1‑101 (MQ=255)
                                                                                          gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAgc            <  2:5204198/101‑1 (MQ=255)
                                                                                          gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAgc            <  1:8913235/101‑1 (MQ=255)
                                                                                          gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAgc            >  1:9129302/1‑101 (MQ=255)
                                                                                           tGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAgcg           <  1:1165148/101‑1 (MQ=255)
                                                                                           caagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGAAGCCAGAGGGGGGc               <  2:6110797/96‑1 (MQ=18)
                                                                                            gCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAgcgc          >  2:5365/1‑101 (MQ=255)
                                                                                             ccGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGcc         >  2:7204361/1‑101 (MQ=255)
                                                                                              ctggACT.CCCCCGGAATATTTGAGGCGAGATGAA...GGGGGGCGTTCATCTTGCCGAATATATCCCGGGTGGTGAATGGCGCAGCCAGAGGGGGGCATCGccc        <  2:1110049/99‑1 (MQ=255)
                                                                                              cGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGccc        <  2:8191464/101‑1 (MQ=255)
                                                                                               cggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCAAAGCCAGAGGGGGGCAgcc           >  1:4266588/2‑100 (MQ=17)
                                                                                                  gcT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGcccccct    <  1:767696/100‑3 (MQ=255)
                                                                                                   cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGcccccctt   <  2:2795914/101‑4 (MQ=255)
                                                                                                   cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGcccccctt   <  1:2422173/101‑4 (MQ=255)
                                                                                                    t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGccccccttc  <  2:3702144/101‑5 (MQ=255)
                                                                                                    t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTGGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGccccccttc  <  2:1735425/101‑5 (MQ=255)
                                                                                                     |                                                                                                       
ACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCCCTTCG  >  NC_014034/2249201‑2249400

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG