Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,331 +A 2.3% intergenic (‑127/‑178) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3312.→A 2.3% 28.5 ‑2.8 ‑2.0443intergenic (‑127/‑178)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (8/2):  ref base (209/224):  total (217/226)
Fisher's exact test strand distribution p-value = 5.79e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.05e-02

Only 100 of 480 total aligned reads displayed.

GATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGT  >  NC_014034/2249232‑2249430
                                                                                                          |                                                                                                        
 aTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACG.CCCCCGGGATATTTGAGGCAAGATGAA...gggg..g                                                                                                        >  1:4668825/1‑101 (MQ=255)
  tGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gggg..gg                                                                                                       >  2:7811866/1‑101 (MQ=255)
   gCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGc                                                                                                      <  2:2385718/101‑1 (MQ=255)
       aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTc                                                                                                  <  1:6971091/101‑1 (MQ=255)
       aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTc                                                                                                  <  2:1571314/101‑1 (MQ=255)
       aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTc                                                                                                  <  2:748514/101‑1 (MQ=255)
       aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTc                                                                                                  >  1:2398654/1‑101 (MQ=255)
       aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGATCTG.CCCACGTCCCGTGCCGGGACT.CCACAGGGATATTTGAGGCAAGATGAA...GGGG..GGCgtta                                                                                                  >  1:4228236/1‑100 (MQ=38)
        gATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCa                                                                                                 <  1:3150102/101‑1 (MQ=255)
        gATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCa                                                                                                 <  1:4323824/101‑1 (MQ=255)
         aTACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCAt                                                                                                <  1:93810/101‑1 (MQ=255)
            ccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTTAGGCAAGATGAA...GGGG..GGCTTTCATCtt                                                                                             <  2:3577090/101‑1 (MQ=255)
            ccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCtt                                                                                             <  1:5339678/101‑1 (MQ=255)
            ccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCtt                                                                                             <  1:8895606/101‑1 (MQ=255)
            ccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCtt                                                                                             <  1:3813698/101‑1 (MQ=255)
             cGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTg                                                                                            <  2:3266419/101‑1 (MQ=255)
             cGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTg                                                                                            <  1:7712142/101‑1 (MQ=255)
              gCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGAAAGATGAA...GGGG..GGCTTCTCTCTTGc                                                                                           >  2:2583141/1‑101 (MQ=38)
               cAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGGTATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGcc                                                                                          >  1:1532082/1‑101 (MQ=255)
                 gCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGa                                                                                        >  1:5883854/1‑101 (MQ=255)
                  cTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCgca                                                                                       >  2:7852134/1‑99 (MQ=255)
                  cTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaa                                                                                       >  2:1165130/1‑101 (MQ=255)
                   ttGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaaa                                                                                      >  1:7407795/1‑101 (MQ=255)
                    tGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaaaa                                                                                     <  2:4654239/101‑1 (MQ=255)
                      ggCACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAata                                                                                   <  2:8135876/101‑1 (MQ=255)
                        cACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATc                                                                                 <  1:879592/101‑1 (MQ=255)
                        cACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATc                                                                                 <  1:7930419/101‑1 (MQ=255)
                        cACCCGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATc                                                                                 <  2:8818152/101‑1 (MQ=255)
                          cccGGGGCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATccc                                                                               <  1:1004950/101‑1 (MQ=255)
                            cGGGTCAAGAAGGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCgg                                                                             <  2:1065071/101‑1 (MQ=255)
                                  aagaagGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                                       >  1:9048861/1‑101 (MQ=255)
                                  aagaagGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                                       >  2:1420596/1‑101 (MQ=255)
                                  aagaagGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                                       >  1:8341943/1‑101 (MQ=255)
                                   agaagGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGa                                                                      <  1:20988/101‑1 (MQ=255)
                                   agaagGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGa                                                                      <  1:5549844/101‑1 (MQ=255)
                                   agaagGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGa                                                                      >  1:4436970/1‑101 (MQ=255)
                                    gaagGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGaa                                                                     >  1:8178951/1‑101 (MQ=255)
                                     aagGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.t                                                                   >  2:9107635/1‑101 (MQ=255)
                                     aagGGGGCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.t                                                                   >  2:7633092/1‑101 (MQ=255)
                                        ggggCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgc                                                                >  2:8437674/1‑101 (MQ=255)
                                          ggCTCTGCCCC.CGTCCCGCGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgcgc                                                              <  1:2973/101‑1 (MQ=255)
                                           gCTCTGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCa                                                             >  2:5290302/1‑101 (MQ=255)
                                            ctctGCCCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..g                                                          <  1:3544508/101‑1 (MQ=255)
                                              ctgcccc.cGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..Gcc                                                        >  1:7867319/5‑101 (MQ=255)
                                                gcccc.cGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCag                                                      >  1:1654059/3‑101 (MQ=255)
                                                gcccc.cGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCag                                                      >  2:3037958/3‑101 (MQ=255)
                                                gcccc.cGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCag                                                      >  1:2691587/3‑101 (MQ=255)
                                                gcccc.cGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCag                                                      <  1:7663264/99‑1 (MQ=255)
                                                 cccc.cGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCaga                                                     >  1:7602815/2‑101 (MQ=255)
                                                  ccc.cGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCagag                                                    <  2:6063452/101‑1 (MQ=255)
                                                    c.cgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAggg                                                  <  1:3352527/101‑1 (MQ=255)
                                                       gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAggggg                                                >  2:9158144/1‑101 (MQ=255)
                                                        tcccgtGCCGGGACT.CCCCCCGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggggg                                               <  1:7246252/101‑1 (MQ=255)
                                                         cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                              >  1:5049873/1‑101 (MQ=255)
                                                         cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                              <  2:5116876/101‑1 (MQ=255)
                                                          ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCa                                             <  2:205796/101‑1 (MQ=255)
                                                            gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgc                                           <  2:2198624/101‑1 (MQ=255)
                                                            gtGACGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgc                                           <  1:2906103/101‑1 (MQ=255)
                                                             caagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGc                                              >  2:6516338/6‑101 (MQ=18)
                                                               ccGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cc                                       >  2:7204361/1‑101 (MQ=255)
                                                                cGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccc                                      >  2:1818101/1‑101 (MQ=255)
                                                                cGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccc                                      >  1:4960159/1‑101 (MQ=255)
                                                                cGGGACG.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccc                                      <  1:2519396/101‑1 (MQ=255)
                                                                 gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cccc                                     <  1:6049399/101‑1 (MQ=255)
                                                                 gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cccc                                     >  1:1470428/1‑101 (MQ=255)
                                                                 gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cccc                                     <  2:5382030/101‑1 (MQ=255)
                                                                 gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTCGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cccc                                     <  1:6355631/101‑1 (MQ=255)
                                                                    actccccccGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGCAGCG.ccc                                      <  1:1177201/97‑1 (MQ=17)
                                                                     cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cccccctt                                 <  2:2776742/101‑4 (MQ=255)
                                                                     cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cccccctt                                 <  1:1372285/101‑4 (MQ=255)
                                                                      t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccccccttc                                <  2:1281900/101‑5 (MQ=255)
                                                                        cccccGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGCG.CCCCCt                                   <  1:1892457/101‑1 (MQ=0)
                                                                        cccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccccccttcg                               <  2:1464103/101‑6 (MQ=255)
                                                                          cccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTACCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTcgcg                              <  1:4004188/101‑1 (MQ=255)
                                                                            cGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGCG.CCCCCtgccc                               >  1:4744183/1‑97 (MQ=0)
                                                                            cGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..NCCAGAGGGGGGCAGCGCCCCCCTTCgcggg                            >  1:655564/1‑101 (MQ=255)
                                                                             gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTGATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCgcgggc                           <  2:6774643/101‑1 (MQ=255)
                                                                                 tatTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGcc                       >  2:3705213/1‑101 (MQ=255)
                                                                                 tatTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGcc                       <  2:5545367/101‑1 (MQ=255)
                                                                                 tatTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGcc                       <  2:3930156/101‑1 (MQ=255)
                                                                                  atTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGCCGGCcg                      >  1:346550/1‑101 (MQ=255)
                                                                                   tttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTAGCGGGCGGCcgc                     >  1:6244415/1‑101 (MQ=255)
                                                                                    ttGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCcgcg                    <  1:6724924/101‑1 (MQ=255)
                                                                                      gAGGCAAGATGAA...GGGG..GTCTTTCATCTTGCCGAAAATATCGCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGAc                  <  2:9023061/101‑1 (MQ=37)
                                                                                      gAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGAc                  <  2:5682043/101‑1 (MQ=255)
                                                                                       aGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGAcg                 >  2:6290077/1‑101 (MQ=255)
                                                                                        ggCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGAcgc                <  2:4060210/101‑1 (MQ=255)
                                                                                         gCAAGATGAA...GGGG..GTCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGAcgcg               <  2:2026013/101‑1 (MQ=255)
                                                                                          cAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGAcgcgc              >  2:4270268/1‑101 (MQ=255)
                                                                                            aGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACgcgcgc            >  2:8583993/1‑101 (MQ=255)
                                                                                               tGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGACGCGACGCGcgccgc         >  1:1764756/1‑101 (MQ=255)
                                                                                                gAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCa        >  1:8075213/1‑101 (MQ=255)
                                                                                                gAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCa        >  2:5877176/1‑101 (MQ=255)
                                                                                                 aa...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCaa       >  2:8296706/1‑101 (MQ=255)
                                                                                                 aa...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGAGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCaa       >  2:6966749/1‑101 (MQ=255)
                                                                                                  a...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc      >  1:578997/1‑101 (MQ=255)
                                                                                                  a...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGCGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCCAc      >  2:7373864/1‑101 (MQ=37)
                                                                                                        gg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAgg   <  1:6664534/101‑1 (MQ=255)
                                                                                                        gg..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAgg   <  2:4959999/101‑1 (MQ=255)
                                                                                                         g..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAggc  >  2:4761170/1‑100 (MQ=255)
                                                                                                          |                                                                                                        
GATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCC.CGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGT  >  NC_014034/2249232‑2249430

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG