Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,391 +C 95.9% intergenic (‑187/‑118) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3911.→C95.9% 37.5 ‑1.2 ‑0.8394intergenic (‑187/‑118)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (204/172):  ref base (11/5):  total (216/178)
Fisher's exact test strand distribution p-value = 3.11e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.59e-01

Only 100 of 468 total aligned reads displayed.

TGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.CC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCGATGTCC  >  NC_014034/2249291‑2249486
                                                                                                           |                                                                                                
tGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg.cg                                                                                                   <  1:1165148/101‑1 (MQ=255)
  ccGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.cc                                                                                                >  2:7204361/1‑101 (MQ=255)
    gggACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.cc.cc                                                                                             >  1:1470428/1‑101 (MQ=255)
        cTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.cc.cccctt                                                                                         >  1:56779/1‑98 (MQ=255)
        cTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.cc.cccctt                                                                                         <  2:2776742/101‑4 (MQ=255)
         tCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.cc.ccccttc                                                                                        <  1:771031/101‑5 (MQ=255)
         tCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.cc.ccccttc                                                                                        <  2:1281900/101‑5 (MQ=255)
         tCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTGGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.cc.ccccttc                                                                                        <  2:1735425/101‑5 (MQ=255)
          cccccGGTATATTTGCGGCAAGATGAAGTGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAG.CG.CC.CCCt                                                                                           <  2:8937294/101‑1 (MQ=2)
          cccccGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.cc.ccccttcg                                                                                       <  2:1464103/101‑6 (MQ=255)
           ccccGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTcgc                                                                                       <  1:8837916/101‑1 (MQ=255)
           ccccGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTcgc                                                                                       >  2:6221132/1‑101 (MQ=255)
            cccGGGATATTTGAGGCAAGATGAAGGGG..GGCTCTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGTGGGGCAG.CGCCC.CCCTTcgcg                                                                                      >  1:6446734/1‑101 (MQ=25)
              cGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAG.CG.CC.CCCtgccc                                                                                       >  1:4744183/1‑97 (MQ=0)
               gggATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCgcgggc                                                                                   <  2:4394653/101‑1 (MQ=255)
               gggATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCgcgggc                                                                                   >  2:3383730/1‑101 (MQ=255)
                   tatTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGcc                                                                               >  2:3705213/1‑101 (MQ=255)
                   tatTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGcc                                                                               <  1:3496917/101‑1 (MQ=255)
                      ttGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCcgcg                                                                            >  2:5853825/1‑101 (MQ=255)
                      ttGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCcgcg                                                                            <  2:7677818/101‑1 (MQ=255)
                       tGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGa                                                                           >  1:965530/1‑101 (MQ=255)
                          ggCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGAcgc                                                                        <  1:1319276/101‑1 (MQ=255)
                            cAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGAcgcgc                                                                      <  2:469638/101‑1 (MQ=255)
                            cAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGAcgcgc                                                                      >  2:559713/1‑101 (MQ=255)
                               gATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGcgcc                                                                   >  2:2293595/1‑101 (MQ=255)
                               gATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGcgcc                                                                   >  2:8188126/1‑101 (MQ=255)
                                   aaGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCaa                                                               >  2:6865349/1‑101 (MQ=255)
                                   aaGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGAGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCaa                                                               >  2:6966749/1‑101 (MQ=255)
                                    aGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                                                              >  2:7557792/1‑101 (MQ=255)
                                    aGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                                                              >  1:578997/1‑101 (MQ=255)
                                    aGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGCGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCCAc                                                              >  2:7373864/1‑101 (MQ=37)
                                       gg..gcCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCCC.CC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAgg                                                           <  1:4226183/101‑1 (MQ=37)
                                        g..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAggc                                                          >  1:5012030/1‑100 (MQ=255)
                                        g..ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGt                                                          >  2:8374043/1‑101 (MQ=255)
                                           ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTc                                                         <  1:7633419/101‑1 (MQ=255)
                                           ggCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTc                                                         >  2:6327927/1‑101 (MQ=255)
                                           ggCGTTCATCTGCCCGAAAATATCGCGGGGGGTGAA.TGGCGCA..GCGAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTc                                                         <  2:1852102/101‑1 (MQ=37)
                                            gCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCg                                                        >  2:2052331/1‑101 (MQ=255)
                                              tttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTg                                                      <  2:2085355/101‑1 (MQ=255)
                                               ttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGc                                                     >  2:6634731/1‑101 (MQ=255)
                                               ttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGc                                                     <  1:4126757/101‑1 (MQ=255)
                                                  aTCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcccc                                                  >  2:5970751/1‑101 (MQ=255)
                                                   tCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGGCGTGCCCCt                                                 >  1:6734588/1‑101 (MQ=255)
                                                    cTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                                                >  2:2621048/1‑101 (MQ=255)
                                                    cTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                                                <  1:5483855/101‑1 (MQ=255)
                                                    cTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                                                >  1:826460/1‑101 (MQ=255)
                                                    cTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                                                >  2:3901468/1‑101 (MQ=255)
                                                     ttGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCttt                                               <  1:8448028/101‑1 (MQ=255)
                                                      tGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTg                                              >  2:1101625/1‑101 (MQ=255)
                                                       gCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGt                                             >  2:6578709/1‑101 (MQ=255)
                                                        ccGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCTTGCCCCTTTGTc                                            <  1:6630177/101‑1 (MQ=255)
                                                        ccGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTc                                            <  2:1336230/101‑1 (MQ=255)
                                                          gAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt                                          <  1:3216960/101‑1 (MQ=255)
                                                          gAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt                                          <  1:537564/101‑1 (MQ=255)
                                                           aaaaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAtt                                         <  2:5073078/101‑1 (MQ=255)
                                                            aaaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTc                                        >  2:3216955/1‑101 (MQ=255)
                                                            aaaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTc                                        <  1:7456494/101‑1 (MQ=255)
                                                            aaaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTc                                        >  2:5333992/1‑101 (MQ=255)
                                                             aaTATCCCGGGGGGTGAA.TGTCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCt                                       <  1:7204665/101‑1 (MQ=255)
                                                             aaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCt                                       <  1:168561/101‑1 (MQ=255)
                                                               tatCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGt                                     >  1:6919818/1‑101 (MQ=255)
                                                                tgcccGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTc                                    <  2:2944002/99‑1 (MQ=255)
                                                                 tCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCg                                   <  1:3667832/101‑1 (MQ=255)
                                                                 tCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCg                                   <  2:4592916/101‑1 (MQ=255)
                                                                    cGGGGGCTGAA.TGGGGAA..GCTAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAc                                <  2:5507599/101‑1 (MQ=255)
                                                                      gggggTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAccc                              <  2:8787034/101‑1 (MQ=255)
                                                                      gggggTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAccc                              >  2:3603947/1‑101 (MQ=255)
                                                                       ggggTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCCTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCa                             >  2:8004797/1‑101 (MQ=255)
                                                                       gggaTGAA.TGGCGCA..GCCAGAGGGGGCCAG.CGCCC.CCCTTCGCGGGCGCCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCa                             <  1:2402349/101‑1 (MQ=255)
                                                                        gggTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCAt                            >  1:1937563/1‑101 (MQ=255)
                                                                        gggTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCAt                            >  2:5881813/1‑101 (MQ=255)
                                                                          gTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGt                          <  2:4758918/101‑1 (MQ=255)
                                                                           tGAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTc                         >  2:4106283/1‑101 (MQ=255)
                                                                           tGAA.TGGCGCA..GCCAGAGGGGGCCAG.GGCCC.CCCTCCGCGGGCGGCCGCGGCGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTc                         <  1:2692013/101‑1 (MQ=255)
                                                                            gAA.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCa                        <  2:7866986/101‑1 (MQ=255)
                                                                             aa.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCAt                       <  1:7945283/101‑1 (MQ=255)
                                                                              aagggCGCA..GCCAGAGGGGGGCCG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCAt                       <  1:3306121/98‑1 (MQ=255)
                                                                              a.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATa                      >  2:4861592/1‑101 (MQ=255)
                                                                              a.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATa                      <  1:2049720/101‑1 (MQ=255)
                                                                              a.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATa                      <  2:8597677/101‑1 (MQ=255)
                                                                              a.TGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATa                      >  1:2255843/1‑101 (MQ=255)
                                                                               agggCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATa                      <  1:6240183/99‑1 (MQ=255)
                                                                                tGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATaa                     >  2:2488691/1‑101 (MQ=255)
                                                                                tGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATaa                     >  1:4361087/1‑101 (MQ=255)
                                                                                tGGCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATaa                     <  1:6282056/101‑1 (MQ=255)
                                                                                 ggCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCaagat                    >  1:5295185/1‑97 (MQ=255)
                                                                                 ggCGCA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAg                    <  1:9158566/101‑1 (MQ=255)
                                                                                 ggCGCA..GCCAGAGGGGGGCAG.CG.CCTCCCTGCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAg                    <  2:1532035/101‑1 (MQ=255)
                                                                                    gcA..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTg                 >  1:3831310/1‑101 (MQ=255)
                                                                                      a..GCCAGAGGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTTGc               >  2:9118275/1‑101 (MQ=255)
                                                                                            agagGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCa           >  2:7142746/1‑101 (MQ=255)
                                                                                            agagGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCa           >  1:3035056/1‑101 (MQ=255)
                                                                                              agGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATc         >  1:8518632/1‑101 (MQ=255)
                                                                                              agGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATc         <  1:6029791/101‑1 (MQ=255)
                                                                                              agGGGGGCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATc         >  1:1024218/1‑101 (MQ=255)
                                                                                               ggggggCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCg        >  1:7329918/1‑101 (MQ=255)
                                                                                               ggggggCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCg        <  1:4861752/101‑1 (MQ=255)
                                                                                                 ggggCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCGAt      <  1:8726684/101‑1 (MQ=255)
                                                                                                   ggCAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCGATGt    <  1:7855400/101‑1 (MQ=255)
                                                                                                     cAG.CGCCC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCGATGTcc  <  1:2890739/101‑1 (MQ=255)
                                                                                                           |                                                                                                
TGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.CG.CC.CCCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCGATGTCC  >  NC_014034/2249291‑2249486

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG