Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,304,896 +C 100% coding (2679/2808 nt) RCAP_rcc02139 ← methyl‑accepting chemotaxis protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,304,8961.→C100.0% 1042.8 299coding (2679/2808 nt)RCAP_rcc02139methyl‑accepting chemotaxis protein
Reads supporting (aligned to +/- strand):  new base (126/172):  ref base (1/0):  total (127/172)

Only 100 of 366 total aligned reads displayed.

GGGCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCGCCCTGCATGC.GAAGACCG.CGTACGCTCCCGCA.GGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTG  >  NC_014034/2304796‑2304988
                                                                                                      |                                                                                             
 gggaaGATCCTGATAATCTTGCGCAAAACGCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACcg.c                                                                                              <  1:1519685/98‑1 (MQ=255)
 ggCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGGGGCG.CCTGCATGCGGAAGACcg.c                                                                                              >  2:2999247/1‑101 (MQ=255)
    aaGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACcg.ccgt                                                                                           >  2:7897061/1‑98 (MQ=255)
    aaGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACcg.ccgt                                                                                           >  1:4617097/1‑98 (MQ=255)
     aGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACcg.ccgtc                                                                                          >  1:8387979/1‑97 (MQ=255)
       aTCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCCTGCGGAAGACcg.ccggcag                                                                                        >  2:1365095/1‑95 (MQ=255)
        tCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACcg.ccgtcagc                                                                                       <  2:3947745/101‑8 (MQ=255)
         ccTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACcg.ccgtcagcc                                                                                      >  1:1414354/1‑93 (MQ=255)
          cTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACcg.ccgtcagctc                                                                                     >  1:7061121/1‑92 (MQ=255)
          cTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACcg.ccatcagctc                                                                                     >  1:4911976/1‑92 (MQ=255)
           tGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACcg.ccgtcagctcc                                                                                    <  2:7717329/101‑11 (MQ=255)
           tGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACcg.ccggcagctcc                                                                                    >  2:752587/1‑91 (MQ=255)
              tagtCTGGCGCAAACCTCGGCGGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGc                                                                                  <  1:9074400/98‑1 (MQ=255)
               aaTCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCGCCC.GCATGCGGAAGACCGCCGTCAGCTCCCGCa                                                                                 >  1:3050522/1‑101 (MQ=255)
                aTCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCA.g                                                                               >  2:2190018/1‑101 (MQ=255)
                 tCTTGAGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCA.gg                                                                              >  2:4528818/1‑101 (MQ=255)
                  cTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCA.ggg                                                                             >  2:4989592/1‑100 (MQ=255)
                   ttGCGCAAACCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCA.gggt                                                                            <  2:5456861/101‑3 (MQ=255)
                     gcgcAAACCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGCCCGCCGTCAGCTCCCGCA.gggtct                                                                          <  2:6101454/101‑5 (MQ=255)
                     gcgcAAAACCCGGCCGGACCCGGCCTGCCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCA.gggtct                                                                          <  1:2664242/101‑5 (MQ=255)
                      cgcAAACCCCGGCCGGAGCCGGCCGGCCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCA.gggtctg                                                                         <  2:5233401/101‑6 (MQ=255)
                      cgcAAAACCGGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCA.gggtctg                                                                         <  2:3572788/101‑6 (MQ=255)
                       gcAAACCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTgc                                                                         <  1:6393831/101‑1 (MQ=255)
                       gcAAAACGCGGCCGGACCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTgc                                                                         <  2:6708216/101‑1 (MQ=255)
                        cAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTgcg                                                                        <  1:1660642/101‑1 (MQ=255)
                        cAAAACTCGGCCGGATCCGGCCTGGCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTgcg                                                                        <  1:3792328/101‑1 (MQ=255)
                         aaaaCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTgcgc                                                                       >  2:3779363/1‑101 (MQ=255)
                         aaaaCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCA.gggcctgcgc                                                                      >  1:7482262/1‑93 (MQ=255)
                          aaaCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGcc                                                                      >  1:1686965/1‑101 (MQ=255)
                          aaaCTCGGCCGGATCCGGACTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGcc                                                                      <  1:102150/101‑1 (MQ=255)
                           acctggcccGGCTCCGGCCTGCCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCt                                                                     <  2:3255214/94‑1 (MQ=255)
                           aaCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGccc                                                                     >  2:8888005/1‑100 (MQ=255)
                           aaCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCt                                                                     >  1:8216672/1‑101 (MQ=255)
                            aCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGGCTGCGCCTc                                                                    >  2:6660250/1‑101 (MQ=255)
                             cgcGGCCGGCTCCGGCCGCTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCg                                                                   <  2:4290459/99‑1 (MQ=255)
                             cTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCg                                                                   >  1:4189500/1‑101 (MQ=255)
                               cGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGcc                                                                 >  2:1686357/1‑101 (MQ=255)
                                ggCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGGGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGccc                                                                >  2:1632196/1‑101 (MQ=255)
                                ggCCGGACCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGccc                                                                <  1:2257703/101‑1 (MQ=255)
                                gcccgtctCCGGCCGGACAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGccc                                                                <  2:5666626/94‑1 (MQ=255)
                                 gcgggACCCGGCCTGTCGGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCca                                                               <  1:2182224/98‑1 (MQ=255)
                                 cccGGAGCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCca                                                               <  2:8965645/100‑1 (MQ=255)
                                  ccGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCcag                                                              >  2:1208074/1‑101 (MQ=255)
                                  ccGGACCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCcag                                                              <  1:8705843/101‑1 (MQ=255)
                                   cGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCcagc                                                             <  1:7897394/101‑1 (MQ=255)
                                    ggACCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCcagca                                                            <  2:3028140/101‑1 (MQ=255)
                                     gATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCcagcag                                                           >  1:5938337/1‑101 (MQ=255)
                                          ggCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCt                                                      >  1:6864174/1‑101 (MQ=255)
                                          ggCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCt                                                      >  1:1084014/1‑101 (MQ=255)
                                          ggCCGGTCAGCCGGCCTGCCGCAACCGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCt                                                      <  2:1463589/101‑1 (MQ=255)
                                           gCCTGTCGGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTg                                                     <  1:76580/101‑1 (MQ=255)
                                           gCCTGCCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTg                                                     <  2:201175/101‑1 (MQ=255)
                                             cTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCg                                                   <  1:5117979/101‑1 (MQ=255)
                                             cTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCg                                                   >  1:8970671/1‑101 (MQ=255)
                                               gccAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTc                                                 <  2:268672/99‑1 (MQ=255)
                                               gTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTc                                                 >  1:992621/1‑101 (MQ=255)
                                                  aGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTcgcg                                              <  1:5272717/101‑1 (MQ=255)
                                                    ccGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGc                                            >  2:6009684/1‑101 (MQ=255)
                                                      ggCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCcg                                          >  2:2542892/1‑101 (MQ=255)
                                                       gCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCcgc                                         >  1:1539097/1‑101 (MQ=255)
                                                         cTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGt                                       <  2:5084183/101‑1 (MQ=255)
                                                          tGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGtt                                      <  2:8092205/101‑1 (MQ=255)
                                                           gCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTGGCCCAGCAGCGCCTGCGTCGCGGCCGCGttt                                     >  1:2953717/1‑101 (MQ=255)
                                                           gCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGttt                                     >  2:6435838/1‑101 (MQ=255)
                                                            ccGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGtttt                                    <  1:1764701/101‑1 (MQ=255)
                                                             cGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTc                                   <  1:7535979/101‑1 (MQ=255)
                                                             cGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTc                                   <  1:77916/101‑1 (MQ=255)
                                                              gCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTcc                                  <  1:1973791/101‑1 (MQ=255)
                                                               cAACGGCGGCTCTGGTGGCG.CCTGCATGCGGAGGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCt                                 <  2:5310792/101‑1 (MQ=255)
                                                               cAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCt                                 >  2:7076425/1‑101 (MQ=255)
                                                                 aCGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCg                               >  1:6671010/1‑101 (MQ=255)
                                                                  cggcggCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGa                              <  1:5542778/101‑1 (MQ=255)
                                                                    gcggcgCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAAc                            <  2:5886228/101‑1 (MQ=255)
                                                                    gcggcgCCGGTGGCG.CCTGCATGCGGAAGACCGCCGGCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCTTTTTCCTCGAAc                            <  1:8118561/101‑1 (MQ=255)
                                                                     cggcgCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACa                           <  2:2810466/101‑1 (MQ=255)
                                                                     cggcgCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACa                           <  1:437104/101‑1 (MQ=255)
                                                                      ggcgCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACAt                          <  2:4810572/101‑1 (MQ=255)
                                                                      ggcgCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACAt                          <  1:8566895/101‑1 (MQ=255)
                                                                      ggcgCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACAt                          <  2:6810175/101‑1 (MQ=255)
                                                                       gcgcCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATc                         <  2:6596986/101‑1 (MQ=25)
                                                                        cgcCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcg                        <  2:4678162/101‑1 (MQ=25)
                                                                        cgcCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcg                        <  2:7372925/101‑1 (MQ=25)
                                                                         gcCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgc                       <  2:9162087/101‑1 (MQ=25)
                                                                          ccGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgcc                      >  2:8012077/1‑101 (MQ=25)
                                                                           cGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccg                     >  1:7282928/1‑101 (MQ=25)
                                                                           cGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccg                     >  2:2345715/1‑101 (MQ=25)
                                                                           cGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccg                     >  1:6173475/1‑101 (MQ=25)
                                                                           cGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccg                     >  2:8753099/1‑101 (MQ=25)
                                                                             gTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTGCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCa                   >  1:6464198/1‑101 (MQ=21)
                                                                                ggcgccTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCAtt                 <  2:4009739/97‑1 (MQ=25)
                                                                                ggcgccTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCAtt                 <  2:3465029/97‑1 (MQ=25)
                                                                                ggcgccTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCAtt                 <  1:805766/97‑1 (MQ=25)
                                                                                 gcgccTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtg                <  2:1724155/98‑1 (MQ=25)
                                                                                  cgccTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtgt               <  1:3582450/99‑1 (MQ=25)
                                                                                   gccTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtgtg              >  2:6334552/2‑101 (MQ=21)
                                                                                      tGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTg           >  2:1157991/1‑101 (MQ=21)
                                                                                       gCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTgg          <  1:8884612/101‑1 (MQ=21)
                                                                                       gCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTgg          >  1:5858251/1‑101 (MQ=21)
                                                                                          tgcggAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTg       <  1:3627793/97‑1 (MQ=25)
                                                                                              gAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCt   <  2:7808071/101‑1 (MQ=25)
                                                                                                      |                                                                                             
GGGCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCGCCCTGCATGC.GAAGACCG.CGTACGCTCCCGCA.GGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTG  >  NC_014034/2304796‑2304988

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG