Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,304,910 +G 100% coding (2665/2808 nt) RCAP_rcc02139 ← methyl‑accepting chemotaxis protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,304,9101.→G100.0% 1102.1 318coding (2665/2808 nt)RCAP_rcc02139methyl‑accepting chemotaxis protein
Reads supporting (aligned to +/- strand):  new base (134/184):  ref base (0/0):  total (134/184)

Only 100 of 352 total aligned reads displayed.

AATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCGCCCTGCATGC.GAAGACCG.CGTACGCTCCCGCA.GGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCACCG  >  NC_014034/2304811‑2305005
                                                                                                      |                                                                                               
 aTCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCA.g                                                                                                >  2:3582342/1‑101 (MQ=255)
 aTCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCA.g                                                                                                >  2:2190018/1‑101 (MQ=255)
  tCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCACA.gg                                                                                               <  1:7806393/101‑1 (MQ=255)
  tCTTGCGCAAAACCGGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCA.gg                                                                                               <  2:4716069/101‑1 (MQ=255)
    ttGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCA.gggt                                                                                             <  2:8776881/101‑3 (MQ=255)
     tGCGCAAACCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCA.gggtc                                                                                            <  1:1672725/101‑4 (MQ=255)
      gcgcAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCA.gggtct                                                                                           >  1:8358274/1‑97 (MQ=255)
       cgcAAACCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCA.gggtctg                                                                                          <  2:5014612/101‑6 (MQ=255)
        ccaaacccgggCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTgc                                                                                          <  2:5290427/92‑1 (MQ=255)
          aaaaCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTgcgc                                                                                        >  2:4513041/1‑101 (MQ=255)
          aaaaCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTgcgc                                                                                        >  1:1355273/1‑101 (MQ=255)
           aaaCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGcc                                                                                       >  1:816765/1‑101 (MQ=255)
           aaaCTCGGCCGGATCCGGACTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGcc                                                                                       <  1:102150/101‑1 (MQ=255)
            acctggcccGGCTCCGGCCTGCCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCt                                                                                      <  2:3255214/94‑1 (MQ=255)
             cccgggCCGGACCCGCCCTGCCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTc                                                                                     <  2:6685275/97‑1 (MQ=255)
              cgcGGCCGGCTCCGGCCGCTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCg                                                                                    <  2:4290459/99‑1 (MQ=255)
              cgcGGCCGGATCCGGCCTGCCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCg                                                                                    <  1:1878866/99‑1 (MQ=255)
              cTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCg                                                                                    >  1:2810565/1‑101 (MQ=255)
                 ggCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCCGCTCCCGCAGGGTCTGCGCCTCGccc                                                                                 >  1:5874127/1‑101 (MQ=255)
                 ggCCGGACCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGccc                                                                                 <  1:2257703/101‑1 (MQ=255)
                  gCCGGCCCCGCCCGGCCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCca                                                                                <  1:948272/101‑1 (MQ=255)
                  cccGGAGCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCca                                                                                <  2:8965645/100‑1 (MQ=255)
                   ccGGACCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCcag                                                                               <  1:8705843/101‑1 (MQ=255)
                    cGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCcagc                                                                              >  2:6411364/1‑101 (MQ=255)
                    cGGACCCGGCCGGTCGGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCcagc                                                                              <  2:8070533/101‑1 (MQ=255)
                     ggATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCcagca                                                                             >  2:7942336/1‑101 (MQ=255)
                     ggACCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCcagca                                                                             <  2:3028140/101‑1 (MQ=255)
                      gccccGGCCTGCCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCcagcag                                                                            <  2:5153422/98‑1 (MQ=255)
                       aTCGGGCCTGCCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCagcagc                                                                           <  2:5367206/101‑1 (MQ=255)
                       aTCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCagcagc                                                                           >  2:563165/1‑101 (MQ=255)
                       aTCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCagcagc                                                                           >  1:5194524/1‑101 (MQ=255)
                           ggCCGGTCAGCCGGCCTGCCGCAACCGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCt                                                                       <  2:1463589/101‑1 (MQ=255)
                            gCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTg                                                                      <  2:5760846/101‑1 (MQ=255)
                            gCCTGCCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTg                                                                      <  2:201175/101‑1 (MQ=255)
                             ccTGTCGGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGc                                                                     <  2:6551284/101‑1 (MQ=255)
                              cTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGGCTGCGCCTCGCCCAGCCGCGCCCGCg                                                                    >  1:8953536/1‑101 (MQ=255)
                               tGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGt                                                                   >  2:7844907/1‑101 (MQ=255)
                                gccAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTc                                                                  <  2:268672/99‑1 (MQ=255)
                                gTCGGCCGCCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTc                                                                  <  2:4005765/101‑1 (MQ=255)
                                gTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTc                                                                  >  2:7526180/1‑101 (MQ=255)
                                gTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTCGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTc                                                                  <  1:6259580/101‑1 (MQ=255)
                                  cAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTcgc                                                                <  1:3726351/101‑1 (MQ=255)
                                   aGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTcgcg                                                               >  2:6657045/1‑101 (MQ=255)
                                      cGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGcc                                                            <  1:1979054/101‑1 (MQ=255)
                                       ggCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCcg                                                           >  2:1193754/1‑101 (MQ=255)
                                         ccTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCcgcg                                                         >  2:7791510/1‑101 (MQ=255)
                                            gCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGGGCCTGCGTCGCGGCCGCGttt                                                      >  2:2853751/1‑101 (MQ=255)
                                            gCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGttt                                                      >  2:1651587/1‑101 (MQ=255)
                                            gCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGttt                                                      <  2:4927590/101‑1 (MQ=255)
                                             ccGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGtttt                                                     <  1:1764701/101‑1 (MQ=255)
                                             ccGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGtttt                                                     <  1:7598629/101‑1 (MQ=255)
                                             ccGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGtttt                                                     <  1:2190080/101‑1 (MQ=255)
                                              cGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTc                                                    <  1:7535979/101‑1 (MQ=255)
                                              cGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTc                                                    <  1:77916/101‑1 (MQ=255)
                                                 aaCGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTTCTc                                                 >  2:2752897/1‑101 (MQ=255)
                                                 aaCGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTc                                                 <  1:7468476/101‑1 (MQ=255)
                                                 aaCGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTc                                                 <  1:5275102/101‑1 (MQ=255)
                                                  aCGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCg                                                <  1:7194114/101‑1 (MQ=255)
                                                     gcggcgCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAAc                                             >  1:9023508/1‑101 (MQ=37)
                                                     gcggcgCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAAc                                             <  1:4586417/101‑1 (MQ=255)
                                                       ggcgCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACAt                                           <  1:8566895/101‑1 (MQ=255)
                                                       ggcgCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACAt                                           <  2:6810175/101‑1 (MQ=255)
                                                         cgcCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcg                                         <  2:7372925/101‑1 (MQ=25)
                                                          gcCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgc                                        <  2:5894414/101‑1 (MQ=25)
                                                          gcCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgc                                        <  2:9162087/101‑1 (MQ=25)
                                                            cGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccg                                      >  1:6173475/1‑101 (MQ=25)
                                                            cGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccg                                      <  1:297571/101‑1 (MQ=25)
                                                            cGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccg                                      >  2:2345715/1‑101 (MQ=25)
                                                            cGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccg                                      <  2:8920090/101‑1 (MQ=25)
                                                             ggTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccgc                                     <  2:7035618/101‑1 (MQ=25)
                                                                 ggcgccTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCAtt                                  <  2:3465029/97‑1 (MQ=25)
                                                                 ggcgccTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCAtt                                  <  1:805766/97‑1 (MQ=25)
                                                                  gcgccTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtg                                 <  2:1724155/98‑1 (MQ=25)
                                                                   cgccTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtgt                                <  1:1608924/99‑1 (MQ=25)
                                                                     ccTGCAGGCGGAAGACCGCCGTCAGCTCCCGCAGGGGCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGc                              <  1:4263895/101‑1 (MQ=21)
                                                                       tGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTg                            <  2:4616962/101‑1 (MQ=25)
                                                                        gCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCNNNNNCCTCGAACNNCNNNGNANTGTGCTgg                           >  1:2657755/1‑101 (MQ=16)
                                                                        gCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTgg                           >  1:5858251/1‑101 (MQ=21)
                                                                         cATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGcgcgg                          >  2:2429681/1‑97 (MQ=21)
                                                                         cATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTggg                          <  1:1524740/101‑1 (MQ=25)
                                                                             cggAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGAc                      <  2:1289973/99‑1 (MQ=21)
                                                                               gAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGTCGCATTGGGCTGGGTGACCt                    <  1:6647416/101‑1 (MQ=21)
                                                                                aaGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTg                   <  1:7951917/101‑1 (MQ=21)
                                                                                aaGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTg                   <  2:3777709/101‑1 (MQ=21)
                                                                                   accgccgtcagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATc                <  1:1100335/91‑1 (MQ=21)
                                                                                      gccgtcagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAg             >  2:6014699/8‑101 (MQ=25)
                                                                                        cgtcagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCt           <  1:6436087/96‑1 (MQ=255)
                                                                                         gtcagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTg          <  1:4024324/97‑1 (MQ=255)
                                                                                         gtcagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTg          >  1:7024859/5‑101 (MQ=255)
                                                                                           cagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGt        >  2:6195709/3‑101 (MQ=255)
                                                                                           cagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGt        <  1:6698149/99‑1 (MQ=255)
                                                                                            agCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGTTGACCTGATCAAGCTGGtt       >  1:3658171/2‑101 (MQ=255)
                                                                                            agCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCG.TTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTc      >  1:3562962/2‑101 (MQ=21)
                                                                                              cTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCa     <  2:413121/101‑1 (MQ=255)
                                                                                               tCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCAc    <  1:4897659/101‑1 (MQ=255)
                                                                                               tCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCAc    >  2:6123357/1‑101 (MQ=255)
                                                                                               tCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCAc    <  2:8357911/101‑1 (MQ=255)
                                                                                               tCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCAc    <  2:1132574/101‑1 (MQ=255)
                                                                                                cccGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCAcc   <  1:568962/101‑1 (MQ=255)
                                                                                                 ccGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCAccg  <  1:3186109/101‑1 (MQ=255)
                                                                                                      |                                                                                               
AATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCGCCCTGCATGC.GAAGACCG.CGTACGCTCCCGCA.GGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCACCG  >  NC_014034/2304811‑2305005

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG