Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_014034 | 2,330,175 | +GGGGCTC | 100% | intergenic (+150/+162) | RCAP_rcc02161 → / ← mrcA | hypothetical protein/penicillin‑binding protein 1A |
Read alignment evidence... | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | position | change | freq | score | reads | annotation | genes | product | ||
* | NC_014034 | 2,330,175 | 1 | .→G | 100.0% | 102.8 | 169 | intergenic (+150/+162) | RCAP_rcc02161/mrcA | hypothetical protein/penicillin‑binding protein 1A |
Reads supporting (aligned to +/- strand): new base (49/88): ref base (2/29): total (51/118) | ||||||||||
Fisher's exact test strand distribution p-value = 9.21e-04 | ||||||||||
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00 | ||||||||||
* | NC_014034 | 2,330,175 | 2 | .→G | 100.0% | 102.8 | 169 | intergenic (+150/+162) | RCAP_rcc02161/mrcA | hypothetical protein/penicillin‑binding protein 1A |
Reads supporting (aligned to +/- strand): new base (49/88): ref base (2/29): total (51/118) | ||||||||||
Fisher's exact test strand distribution p-value = 9.21e-04 | ||||||||||
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00 | ||||||||||
* | NC_014034 | 2,330,175 | 3 | .→G | 100.0% | 102.8 | 169 | intergenic (+150/+162) | RCAP_rcc02161/mrcA | hypothetical protein/penicillin‑binding protein 1A |
Reads supporting (aligned to +/- strand): new base (49/88): ref base (2/29): total (51/118) | ||||||||||
Fisher's exact test strand distribution p-value = 9.21e-04 | ||||||||||
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00 | ||||||||||
* | NC_014034 | 2,330,175 | 4 | .→G | 100.0% | 106.8 | 169 | intergenic (+150/+162) | RCAP_rcc02161/mrcA | hypothetical protein/penicillin‑binding protein 1A |
Reads supporting (aligned to +/- strand): new base (49/88): ref base (2/30): total (51/118) | ||||||||||
Fisher's exact test strand distribution p-value = 5.48e-04 | ||||||||||
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00 | ||||||||||
* | NC_014034 | 2,330,175 | 5 | .→C | 100.0% | 108.2 | 169 | intergenic (+150/+162) | RCAP_rcc02161/mrcA | hypothetical protein/penicillin‑binding protein 1A |
Reads supporting (aligned to +/- strand): new base (47/88): ref base (2/30): total (51/118) | ||||||||||
Fisher's exact test strand distribution p-value = 9.52e-04 | ||||||||||
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00 | ||||||||||
* | NC_014034 | 2,330,175 | 6 | .→T | 100.0% | 103.8 | 169 | intergenic (+150/+162) | RCAP_rcc02161/mrcA | hypothetical protein/penicillin‑binding protein 1A |
Reads supporting (aligned to +/- strand): new base (49/88): ref base (2/30): total (51/118) | ||||||||||
Fisher's exact test strand distribution p-value = 5.48e-04 | ||||||||||
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00 | ||||||||||
* | NC_014034 | 2,330,175 | 7 | .→C | 100.0% | 108.2 | 169 | intergenic (+150/+162) | RCAP_rcc02161/mrcA | hypothetical protein/penicillin‑binding protein 1A |
Reads supporting (aligned to +/- strand): new base (49/88): ref base (2/30): total (51/118) | ||||||||||
Fisher's exact test strand distribution p-value = 5.48e-04 | ||||||||||
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00 |
Only 100 of 190 total aligned reads displayed.
CGGCTTGGCCTCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....G.......CGG....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.GGGGCCCCGGATCACCGG > NC_014034/2330075‑2330246
|||||||
gCTTGGCCTCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGgc....ggg > 1:1800644/1‑99 (MQ=255)
cTTGTCCTCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....G.......Ggg < 2:2397268/101‑1 (MQ=255)
cTTGGCCTCCGCCAAGGGCGGCAACGGCGCTCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....G.......Ggg < 1:9054214/101‑1 (MQ=255)
ggCCTCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....G.......Gggggg > 1:4350971/1‑98 (MQ=255)
ggCCTCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....G.......Ggggct < 1:44964/101‑4 (MQ=255)
ggCCTCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....G.......Ggggct < 1:8442233/101‑4 (MQ=255)
gCCTCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....G.......Ggggggg > 1:678824/1‑97 (MQ=255)
gCCTCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....G.......Ggggctc < 2:8853512/101‑5 (MQ=255)
gCCTCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....G.......Ggggctc < 2:7075133/101‑5 (MQ=255)
gCCTCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....G.......Ggggctc < 1:4140367/101‑5 (MQ=255)
ccTCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....G.......Ggggctcc < 2:3619556/101‑6 (MQ=255)
ccTCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....G.......Ggggctcc < 1:7270785/101‑6 (MQ=255)
ccGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....G.......Ggggctccgcc < 1:2389370/101‑9 (MQ=255)
gCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....G.......Ggggctccgcccc < 2:6895057/101‑11 (MQ=37)
cAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCCGGCG...G.......GGG....CTCCGCCcccc > 2:7866671/1‑101 (MQ=37)
aGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....G.......GGG....GCTCCCCccccccc > 1:3512954/1‑99 (MQ=37)
gcAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTgc > 2:3005528/1‑101 (MQ=18)
agagCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGGTCCGC....CCCCGCACCCCCGGCGTATTTgcgc > 1:7394452/1‑101 (MQ=21)
gccgcACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGa < 1:8961108/101‑1 (MQ=18)
ccgcACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGaa < 1:487212/101‑1 (MQ=18)
ccgcACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGaa < 2:6086929/101‑1 (MQ=18)
ccgcACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGaa < 2:2919808/101‑1 (MQ=18)
ccgcACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGaa < 2:3910292/101‑1 (MQ=18)
ccgcACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGaa < 2:7548481/101‑1 (MQ=18)
cgcACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGaaa < 1:7900413/101‑1 (MQ=18)
cgcACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGaaa < 1:811931/101‑1 (MQ=18)
cgcACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGaaa < 2:4738162/101‑1 (MQ=18)
gccgggATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTA.TTGCGCCAAGaaaaa < 2:4358014/97‑1 (MQ=12)
gcACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGaaaa > 1:6924632/1‑101 (MQ=18)
gcACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGaaaa < 1:7526343/101‑1 (MQ=18)
cACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGaaaaa < 1:2247517/101‑1 (MQ=17)
ggATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGGCAAAGcc > 2:1600435/1‑101 (MQ=12)
gATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCg > 2:1456266/1‑101 (MQ=17)
gATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCg > 2:2056584/1‑101 (MQ=17)
cGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCgggg < 1:3198045/101‑1 (MQ=17)
cGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCgggg < 2:1048231/101‑1 (MQ=17)
cGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGCATTTGCGCCCAGAAAAAGCCgggg > 2:1722849/1‑101 (MQ=12)
ggCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGgc < 2:3926340/101‑1 (MQ=17)
ggCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGgc < 2:5904020/101‑1 (MQ=17)
gCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGgcg < 1:6470358/101‑1 (MQ=17)
cGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGgcgcg < 1:8364928/101‑1 (MQ=17)
cGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGgcgcg > 2:6432431/1‑101 (MQ=17)
gATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGgcgcgc > 2:1913684/1‑101 (MQ=17)
aTGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCa > 1:3918868/1‑101 (MQ=17)
tGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCAt > 1:4392142/1‑101 (MQ=17)
tGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCAt < 2:3369455/101‑1 (MQ=17)
tGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGGATTTGCGCCCAGAAAAAGCCGGGGCGGGCag > 2:4125974/1‑100 (MQ=12)
tGGCGGACAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCAt < 2:6551658/101‑1 (MQ=17)
ggCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCTGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATg < 1:8552192/101‑1 (MQ=17)
ggCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATg < 1:8523836/101‑1 (MQ=17)
ggCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATg < 1:7614737/101‑1 (MQ=17)
ggCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATg < 1:75047/101‑1 (MQ=17)
ggCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATg > 1:3389855/1‑101 (MQ=17)
ggCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATg < 1:6689945/101‑1 (MQ=17)
ggCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATg < 2:7728610/101‑1 (MQ=17)
ggCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCC.GGGCGCGCATGc < 1:2128357/101‑1 (MQ=12)
gCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGc < 2:87974/101‑1 (MQ=18)
ggAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.gg > 2:8122467/1‑101 (MQ=17)
ggAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.gg < 1:1643072/101‑1 (MQ=17)
ggAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.gg > 1:4576587/1‑101 (MQ=17)
ggAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAACCGGGGCGCGCATGC.gg > 1:1918111/1‑101 (MQ=14)
ggAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGGATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.gg > 1:5670504/1‑101 (MQ=17)
gagaGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.ggg > 1:7329465/1‑101 (MQ=17)
agagGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGGTCCGC....CCCCCCAACCCCCGCGGATTTGCGCCAAAAAAAAGCCGGGGCGCGCATGC.gggg > 1:2514825/1‑101 (MQ=12)
agagGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.gggg < 1:2544016/101‑1 (MQ=17)
agGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.GGGGcc > 1:2722917/1‑101 (MQ=17)
agGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.GGGGcc > 2:8635043/1‑101 (MQ=17)
ggAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.GGGGccc > 2:1305602/1‑101 (MQ=17)
gAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.GGGGcccc < 2:5224707/101‑1 (MQ=17)
gAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.GGGGcccc < 2:6658794/101‑1 (MQ=17)
gAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.GGGGcccc < 1:1224810/101‑1 (MQ=17)
gAAGTTCGCCGCGCAGGCGCGGCGCCCCGGGCTGGGGCTC....CGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.GGGGcccc < 1:6262510/101‑1 (MQ=12)
aaTTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.GGGGCCCCg < 2:5670297/101‑1 (MQ=17)
aaTTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.GGGGCCCCg < 2:9046953/101‑1 (MQ=17)
aaTTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.GGGGCCCCg > 1:5072806/1‑101 (MQ=17)
aaTTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.GGGGCCCCg < 1:5536594/101‑1 (MQ=17)
aTTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGGCAAGAAAAAGCCGGGGCGCGCATGG.CGGGCCCCgg > 1:9036147/1‑101 (MQ=9)
aTTTCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.GGGGCCCCgg < 2:2193900/101‑1 (MQ=17)
tttCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.GGGGCCCCGGa < 1:1056804/101‑1 (MQ=17)
tttCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.GGGGCCCCGGa > 2:5433419/1‑101 (MQ=17)
tttCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.GGGGCCCCGGa < 2:7789679/101‑1 (MQ=17)
ttCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGGTCCGC....CCCCGCACCCCCCGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGCGGGGGCCCCggc > 2:7208392/1‑100 (MQ=16)
ttCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.GGGGCCCCGGAt > 1:4012579/1‑101 (MQ=17)
ttCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCACGC.GGGGTCCCGGac > 1:5967520/1‑100 (MQ=9)
tCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.GGGGCCCCGGATc < 1:1279434/101‑1 (MQ=18)
tCGCCGCGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.GGGGCCCCGGATc > 2:3649355/1‑101 (MQ=18)
cgccgcGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.GGGGCCCCGGATCa < 1:6971520/101‑1 (MQ=18)
cgccgcGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.GGGGCCCCGGATCa < 2:1541612/101‑1 (MQ=18)
cgccgcGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.GGGGCCCCGGATCa > 1:2282638/1‑101 (MQ=18)
cgccgcGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.GGGGCCCCGGATCa > 2:5315108/1‑101 (MQ=18)
gccgcGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.GGGGCCCCGGATCAc < 2:7144813/101‑1 (MQ=18)
gccgcGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.GGGGCCCCGGATCAc < 2:594405/101‑1 (MQ=18)
ccgcGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGTATGC.GGGGCCCCGGATCAcc < 1:8226039/101‑1 (MQ=17)
ccgcGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.GGGGCCCCGGATCAcc < 1:8972661/101‑1 (MQ=18)
ccgcGCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.GGGGCCCCGGATCAcc < 2:1410211/101‑1 (MQ=18)
cgcgCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.GGGGCCCCGGATCCCcg > 1:2778332/1‑101 (MQ=17)
cgcgCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.GGGGCCCCGGATCACcg < 2:7126351/101‑1 (MQ=18)
cgcgCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.GGGGCCCCGGATCACcg < 1:2854385/101‑1 (MQ=18)
cgcgCAGGCGCGGCGACCCGGGC....GGGGGCTCCGC....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.GGGGCCCCGGATCACcg < 2:8257996/101‑1 (MQ=18)
gcgcAGGCGCGGCGACCCGGGCGGG.G.......CGGCCCCCCCCGCACCCCCCGCGGATTTTGGCCAAGAAAAAGGCGGGGGGCGCACGG.GGGGGCCCggcgcccccc > 1:5927470/1‑93 (MQ=255)
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CGGCTTGGCCTCCGCCAAGGGCGGCAACGGCGCGCAGAGCGCCGCACGGATCGGCCGATGGCGGAGAGGAATTTCGCCGCGCAGGCGCGGCGACCCGGGC....G.......CGG....CCCCGCACCCCCGGCGTATTTGCGCCAAGAAAAAGCCGGGGCGCGCATGC.GGGGCCCCGGATCACCGG > NC_014034/2330075‑2330246
Base quality scores: 
ATCG
< 3 ≤
ATCG
< 18 ≤
ATCG
< 26 ≤
ATCG
< 33 ≤
ATCG
< 40 ≤
ATCG
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