Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,435,308 +C 1.5% intergenic (+17/‑37) RCAP_rcc02259 → / → RCAP_rcc02260 WbqC‑like protein family/type 11 family methyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,435,3081.→C 1.5% 6.7 ‑0.9 ‑1.1336intergenic (+17/‑37)RCAP_rcc02259/RCAP_rcc02260WbqC‑like protein family/type 11 family methyltransferase
Reads supporting (aligned to +/- strand):  new base (4/1):  ref base (192/139):  total (196/140)
Fisher's exact test strand distribution p-value = 4.06e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.91e-02

Only 100 of 456 total aligned reads displayed.

TATACAGGAAAACATACATGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGTCGATCCGCTCGGCCATG  >  NC_014034/2435208‑2435408
                                                                                                     |                                                                                                    
 ataCAGGAAAACATACATGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.c                                                                                                     >  2:3990048/1‑101 (MQ=255)
 ataCAGGAAAACATACATGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.c                                                                                                     >  1:5706043/1‑101 (MQ=255)
    cAGGAAAACATACATGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.cccc                                                                                                  <  1:5316168/101‑1 (MQ=255)
    cAGGAAAACATACATGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.cccc                                                                                                  >  1:8981140/1‑101 (MQ=255)
       gAAAACATACATGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.ccccccc                                                                                               >  1:7958365/1‑101 (MQ=255)
        aaaaCATACATGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.cccccccc                                                                                              >  2:6788325/1‑101 (MQ=255)
         aaaCATACATGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.ccccccccc                                                                                             >  1:6003995/1‑101 (MQ=255)
         aaaCATACATGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.ccccccccc                                                                                             >  1:6404262/1‑101 (MQ=255)
         aaaCATACATGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.ccccccccc                                                                                             >  1:8145018/1‑101 (MQ=255)
          aaCATACATGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.cccccccccc                                                                                            >  2:379172/1‑101 (MQ=255)
           acatacatGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCg                                                                                           >  1:1531382/1‑101 (MQ=255)
           acatacatGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCg                                                                                           >  2:7562097/1‑101 (MQ=255)
              tacatGTTTTCTGCCCGCCCTGTTCGACCGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGaaa                                                                                        <  1:2786723/101‑1 (MQ=255)
                 atGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGGGGGGTTGCGTGCAGACATCTTGGAAGGCTGCCCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATc                                                                                     <  1:2481685/101‑1 (MQ=255)
                 atGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATACGTGTG.CCCCCCCCCCAAAAATc                                                                                     >  1:3246302/1‑101 (MQ=255)
                    ttttCTCCCCGCCCTGTTCGAACGGACCCTGGCGGGGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATcccc                                                                                  <  1:1415394/101‑1 (MQ=255)
                      ttCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGa                                                                                <  1:8241954/101‑1 (MQ=255)
                       tCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCCAAAAACCCCCAg                                                                               >  2:6041823/1‑101 (MQ=255)
                       tCTGCCCGCCCTGTTCGAACGGACCGTGGAGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAg                                                                               <  2:648197/101‑1 (MQ=255)
                        ccgcccgcccTGTTCGAACGGACCGGGGCGGAGTTGCGGGCAGACATCTTGGAAGGCTTACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGc                                                                              <  2:123200/99‑1 (MQ=255)
                        cTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCCAAAACCCCCGAGc                                                                              >  1:6429642/1‑101 (MQ=255)
                        cTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTC.CCCACCCCCCCAAAAACTCCGatc                                                                              >  2:2221628/1‑99 (MQ=255)
                          gcccgcccTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGACCgg                                                                            >  2:4863004/1‑101 (MQ=255)
                           cccgcccTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGga                                                                           >  2:848567/1‑101 (MQ=255)
                           cccgcccTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAAACCCCGacgggc                                                                           >  2:5744436/1‑96 (MQ=255)
                             cgcccTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGgaga                                                                         <  1:8659664/101‑1 (MQ=255)
                             cgcccTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGgaga                                                                         >  2:2289966/1‑101 (MQ=255)
                              gcccTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGCGCGGAGaa                                                                        >  1:422376/1‑101 (MQ=255)
                               cccTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAg                                                                       <  1:957413/101‑1 (MQ=255)
                                ccTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGACCGAAGAAGc                                                                      >  1:1616064/1‑101 (MQ=255)
                                   gTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTc                                                                   >  1:1747286/1‑101 (MQ=255)
                                    ttccagcGGACCGTGTCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAAGCCCCGAGCGGAGAAGCGTcc                                                                  >  1:7243212/7‑101 (MQ=255)
                                    ttCGAACGGACCGGGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTcc                                                                  <  2:7385691/101‑1 (MQ=255)
                                     tCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG..CCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCAt                                                                >  1:191154/1‑101 (MQ=255)
                                        aaCGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGGGAAGCGTcacagg                                                              >  2:5202705/1‑96 (MQ=255)
                                            gcccGTGGCGGAGTTGCGGGCAGACATCTTGGAAGGCTGCCCCTCCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGcccc                                                          <  2:775180/99‑1 (MQ=255)
                                             gccGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCa                                                         <  2:1531335/100‑1 (MQ=255)
                                             gccGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCa                                                         <  1:8572985/100‑1 (MQ=255)
                                             cccGGGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGCCCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCa                                                         <  2:6633930/100‑1 (MQ=255)
                                             aCCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCa                                                         <  2:7242908/101‑1 (MQ=255)
                                              ccGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCaa                                                        >  2:1869026/1‑101 (MQ=255)
                                                gTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTGCCCCCCCCCCCGAAAATCCCCCGGCGGAGAAGCGTCCATGGCCCCaac                                                       >  2:4744052/1‑100 (MQ=255)
                                                gTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGc                                                      <  2:617527/101‑1 (MQ=255)
                                                 gggCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCg                                                     <  1:4300995/100‑1 (MQ=255)
                                                  ggCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGa                                                    >  2:5546754/1‑101 (MQ=255)
                                                  ggCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGACGGGGGAAGCGGCCAGGCCCCCAAGCGa                                                    >  2:4350524/1‑101 (MQ=255)
                                                     ggAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGAccc                                                 >  1:6191809/1‑101 (MQ=255)
                                                     ggAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGAccc                                                 >  1:4976164/1‑101 (MQ=255)
                                                      gAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGGGAAGCGGCCCTGGCCCCAAGCGAcccc                                                >  1:385763/1‑101 (MQ=255)
                                                       ggTTGCGTGCAGACATCTTGGAAGGCCGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCg                                               <  2:3133347/100‑1 (MQ=255)
                                                       agttgctgccAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGGCCATGGCCCCAAGCGAcccca                                               <  2:5840203/92‑2 (MQ=255)
                                                       aGTTGCGTGCATACATCTTGGAAGGCTGCCCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCg                                               <  2:2813493/101‑1 (MQ=255)
                                                           tcGTGCGGTCATTTTGGAAGGCGGTCCCTTCCGATCCGTGGG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGa                                           <  1:2221695/100‑1 (MQ=255)
                                                             gTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCCACCCCGGTGacc                                         >  2:1397948/1‑100 (MQ=255)
                                                              tGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGacac                                        <  1:7588973/101‑1 (MQ=255)
                                                              tGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGacac                                        <  2:5681415/101‑1 (MQ=255)
                                                               gCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACAcc                                       <  1:8290502/101‑1 (MQ=255)
                                                                 aGACATTTTGGAAGGCTGACCCTTCCGATCCGTGTC.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGt                                     <  1:2094506/101‑1 (MQ=255)
                                                                 aGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGGGAAGCGTCCATGGCCCCCACCGACCCCGGCGCCACCgg                                     >  1:1335700/1‑100 (MQ=255)
                                                                 aGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGt                                     >  2:7021239/1‑101 (MQ=255)
                                                                 aGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCCGTGACACCGt                                     >  2:3520523/1‑101 (MQ=255)
                                                                  gcgaTCTTGGAAGCCTGCCCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTg                                    <  2:2460025/98‑1 (MQ=255)
                                                                  gACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTg                                    >  1:7487713/1‑101 (MQ=255)
                                                                  gACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTg                                    >  1:5075947/1‑101 (MQ=255)
                                                                  gACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCCAAAATCCCCGAGCGGGGAAGCGGCCATGGCCCCAAGCGACCCCCGTGGCACCGTg                                    >  2:7881588/1‑101 (MQ=255)
                                                                   aCATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACCcccgga                                   >  2:4224422/1‑97 (MQ=255)
                                                                    cATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCCTGAt                                  >  1:7072341/1‑101 (MQ=255)
                                                                    agtCTTGAGAGGCTGGCCCTTCCGGTCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGAt                                  <  2:5037837/99‑1 (MQ=255)
                                                                     aTCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGGGACACCGCGATg                                 >  1:5794614/1‑101 (MQ=255)
                                                                           gAAGGCTGCCCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGccgccc                           <  1:1018226/101‑1 (MQ=255)
                                                                           gAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGGGACACCGCGATGccgccc                           >  1:3466082/1‑101 (MQ=255)
                                                                            aaGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGGGCCACCGTGATGccgcccc                          >  2:4615425/1‑100 (MQ=255)
                                                                             aGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGGGACCCCGTGATGccgcccgc                         >  2:537349/1‑101 (MQ=255)
                                                                               gcttgcccCTCCGGTCCGGTGG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCt                       <  2:3901736/96‑1 (MQ=255)
                                                                               gCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCt                       >  2:3048954/1‑101 (MQ=255)
                                                                                cgcccccCTCCGACCCGTCCG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCtt                      <  2:2003946/97‑1 (MQ=255)
                                                                                cTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCtt                      >  1:4596897/1‑101 (MQ=255)
                                                                                 tGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTtc                     <  1:5211663/101‑1 (MQ=255)
                                                                                 gagcccTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTtc                     <  2:8970627/98‑1 (MQ=255)
                                                                                  gACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCCAGCGCCCCCGGTGCCCCCGGGGTGCCGCCCGCCtccc                    >  1:2347298/1‑98 (MQ=255)
                                                                                    cccTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTtcgtc                  <  1:4846265/101‑1 (MQ=255)
                                                                                     ccTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTtcgtcg                 <  2:812420/101‑1 (MQ=255)
                                                                                      cTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGGGAAGCGTCCATGGCCCCAAGCGCCCCCGGTGCACCCGTGATGCCGCCCCCCTccgccgc                >  1:6811937/1‑100 (MQ=255)
                                                                                       ttCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGGGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGGGAGACCGGGGTGCCGACCGCCTtcgtcggt               >  2:8370229/1‑99 (MQ=255)
                                                                                        tCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGCCGATc              >  2:4475055/1‑101 (MQ=255)
                                                                                         ccGATCCGTGTGCCCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGTCGATc              <  1:4744201/101‑1 (MQ=255)
                                                                                         ccGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGTCGATcc             <  2:8691634/101‑1 (MQ=255)
                                                                                          cgctCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGTCGATCCg            <  1:6266897/98‑1 (MQ=255)
                                                                                          ccaTCCGTGGG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGTCGATCCg            <  1:6716726/99‑1 (MQ=255)
                                                                                          cGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGTCGATCCg            <  1:4428636/101‑1 (MQ=255)
                                                                                           gATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGTCGATCCGc           >  2:8753358/1‑101 (MQ=255)
                                                                                            aTCCGTGTC.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGTCGATCCGCt          <  1:2996401/101‑1 (MQ=255)
                                                                                            aTCCGAGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGTCGATCCGCt          >  1:5449965/1‑101 (MQ=255)
                                                                                              ccGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGTCGATCCGCTCg        <  1:363214/101‑1 (MQ=255)
                                                                                              ccGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGTCGATCCGCTCg        >  2:8075325/1‑101 (MQ=255)
                                                                                               cGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGTCGATCCGCTCgg       <  2:362484/101‑1 (MQ=255)
                                                                                                gtgtG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGTCGATCCGCTCGGc      <  1:4097234/101‑1 (MQ=255)
                                                                                                 tgtg.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGTCGATCCGCTCGGcc     >  2:3294258/1‑101 (MQ=255)
                                                                                                  gtg.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGTCGATCCGCTCGGCCa    >  1:7877628/1‑101 (MQ=255)
                                                                                                    g.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGTCGATCCCCTCGGCCCTg  >  2:7412475/1‑101 (MQ=255)
                                                                                                     |                                                                                                    
TATACAGGAAAACATACATGTTTTCTGCCCGCCCTGTTCGAACGGACCGTGGCGGAGTTGCGTGCAGACATCTTGGAAGGCTGACCCTTCCGATCCGTGTG.CCCCCCCCCCGAAAATCCCCGAGCGGAGAAGCGTCCATGGCCCCAAGCGACCCCGGTGACACCGTGATGCCGCCCGCCTTCGTCGATCCGCTCGGCCATG  >  NC_014034/2435208‑2435408

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG