Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 243,600 +A 0.6% intergenic (+29/+91) mogA → / ← ubiG molybdopterin biosynthesis mog protein/3‑demethylubiquinone‑9 3‑O‑methyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034243,6001.→A 0.6% 3.8 ‑2.6 ‑0.9527intergenic (+29/+91)mogA/ubiGmolybdopterin biosynthesis mog protein/3‑demethylubiquinone‑9 3‑O‑methyltransferase
Reads supporting (aligned to +/- strand):  new base (3/0):  ref base (222/302):  total (225/302)
Fisher's exact test strand distribution p-value = 7.72e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.32e-01

Only 100 of 562 total aligned reads displayed.

GATCTGATCGGGGCCGGGCATATCGACACCGATCCGGCGAAAATCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGTTTC  >  NC_014034/243500‑243700
                                                                                                        |                                                                                                     
 atctgatcGGGGCCGGGCATATCGACACCGATCCGGCGAAAATCGCGGCGTTTCGCCCTAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.a                                                                                                      <  2:4363858/101‑1 (MQ=255)
 atctgatcGGGGCCGGGCATATCGACACCGATCCGGCGAAAATCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.a                                                                                                      <  1:100062/101‑1 (MQ=255)
  tctgatcGGGGCCGGGCATATCGACACCGATCCGGCGAAAATCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.At                                                                                                     <  2:8343499/101‑1 (MQ=255)
  tctgatcGGGGCCGGGCATATCGACACCGATCCGGCGAAAATCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.At                                                                                                     <  2:3349612/101‑1 (MQ=255)
  tctgatcGGGGCCGGGCATATCGACACCGATCCGGCGAAAATCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.At                                                                                                     <  1:6750293/101‑1 (MQ=255)
   ctgatcGGGGCCGGGCATATCGACACCGATCCGGCGAAAATCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.Att                                                                                                    <  1:4977409/101‑1 (MQ=255)
    tgatcGGGGCCGGGCATATCGACACCGATCCGGCGAAAATCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTc                                                                                                   <  2:9094496/101‑1 (MQ=255)
     gatcGGGGCCGGGCATATCGACACCGATCCGGCGAAAATCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCa                                                                                                  >  2:3231771/1‑101 (MQ=255)
       tcGGGGCCGGGCATATCGACACCGATCCGGCGAAAATCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCAcc                                                                                                <  1:8206591/101‑1 (MQ=255)
          gggCCGGGCATATCGACACCGATCCGGCGAAAATCGCGGCGTTTCTCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCAccccc                                                                                             <  2:7643762/101‑1 (MQ=255)
           ggCCGGGCATATCGACACCGATCCGGCGAAAATCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCg                                                                                            >  2:8121227/1‑101 (MQ=255)
           ggCCGGGCATATCGACACCGATCCGGCGAAAATCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCg                                                                                            <  2:7771312/101‑1 (MQ=255)
           ggCCGGGCATATCGACACCGATCCGGCGAAAATCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCg                                                                                            <  1:4034330/101‑1 (MQ=255)
            gCCGGGCATATCGACACCGATCCGGCGAAAATCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCgg                                                                                           <  1:6913672/101‑1 (MQ=255)
            gCCGGGCATATCGACACCGATCCGGCGAAAATCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCgg                                                                                           <  2:1567247/101‑1 (MQ=255)
             ccGGGCATATCGACACCGATCCGGCGAAAATCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCggg                                                                                          <  2:1245141/101‑1 (MQ=255)
               gggCATATCGACACCGATCCGGCGAAAATCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGat                                                                                        <  2:4002057/101‑1 (MQ=255)
                ggCATATCGACACCGATCCGGCGAAAATCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGata                                                                                       >  2:5450327/1‑101 (MQ=255)
                 gCATATCGACACCGATCCGGCGAAAATCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGatat                                                                                      >  1:3246846/1‑101 (MQ=255)
                 gCATATCGACACCGATCCGGCGAAAATCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGatat                                                                                      <  1:8358887/101‑1 (MQ=255)
                  cATATCGACACCGATCCGGCGAAAATCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATAtt                                                                                     >  2:2605391/1‑101 (MQ=255)
                   atatCGACACCGATCCGGCGAAAATCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATAttt                                                                                    >  2:2534481/1‑101 (MQ=255)
                   atatCGACACCGATCCGGCGAAAATCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATAttt                                                                                    <  2:67389/101‑1 (MQ=255)
                    tatCGACACCGATCCGGCGAAAATCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTg                                                                                   <  2:6493419/101‑1 (MQ=255)
                    tatCGACACCGATCCGGCGAAAATCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTg                                                                                   <  2:2836980/101‑1 (MQ=255)
                     atCGACACCGATCCGGCGAAAATCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGa                                                                                  >  1:1582488/1‑101 (MQ=255)
                      tCGACACCGATCCGGCGAAAATCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAg                                                                                 >  2:1004851/1‑101 (MQ=255)
                        gACACCGATCCGGCGAAAATCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGc                                                                               >  1:4636338/1‑101 (MQ=255)
                            ccGATCCGGCGAAAATCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCCAGa                                                                           <  1:8801335/101‑1 (MQ=255)
                            ccGATCCGGCGAAAATCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGa                                                                           <  1:2595536/101‑1 (MQ=255)
                              gATCCGGCGAAAATCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.a                                                                        >  2:3127609/1‑101 (MQ=255)
                              gATCCGGCGAAAATCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.a                                                                        <  2:1467042/101‑1 (MQ=255)
                               aTCCGGCGAAAATCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGGC.ATTCACCCCCGGGATATTTGAGGCAAGAT.aa                                                                       >  1:6112740/1‑101 (MQ=255)
                               aTCCGGCGAAAATCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.aa                                                                       <  2:5285088/101‑1 (MQ=255)
                                 ccGGCGAAAATCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAg                                                                     <  2:8918187/101‑1 (MQ=255)
                                  cGGCGAAAATCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAgg                                                                    <  2:2998869/101‑1 (MQ=255)
                                  cGGCGAAAATCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAgg                                                                    <  1:9187697/101‑1 (MQ=255)
                                   ggCGAAAATCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGa                                                                   >  1:3664933/1‑101 (MQ=255)
                                       aaaaTCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCt                                                               >  2:2357074/1‑101 (MQ=255)
                                       aaaaTCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCt                                                               >  2:798145/1‑101 (MQ=255)
                                        aaaTCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTg                                                              >  2:1727615/1‑101 (MQ=255)
                                        aaaTCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTg                                                              >  2:5592609/1‑101 (MQ=255)
                                           tCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGttt                                                           <  2:2006250/101‑1 (MQ=255)
                                            cgcgGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGtttt                                                          <  1:5732175/101‑1 (MQ=255)
                                            cgcgGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGtttt                                                          <  1:6484524/101‑1 (MQ=255)
                                            cgcgGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGtttt                                                          <  1:3048540/101‑1 (MQ=255)
                                            cgcgGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGtttt                                                          <  1:1151082/101‑1 (MQ=255)
                                             gcggcgTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTa                                                         >  1:7055709/1‑101 (MQ=255)
                                                gcgTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCt                                                      <  2:6828056/101‑1 (MQ=255)
                                                gcgTGTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCt                                                      <  2:1378872/101‑1 (MQ=255)
                                                  gTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTg                                                    <  1:3766178/101‑1 (MQ=255)
                                                    ttCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGcc                                                  >  2:5924618/1‑101 (MQ=255)
                                                     tCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTTCCt                                                 <  2:3742832/101‑1 (MQ=255)
                                                     tCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCt                                                 <  1:346269/101‑1 (MQ=255)
                                                     tCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCt                                                 <  2:8241977/101‑1 (MQ=255)
                                                       gCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCa                                               <  1:2476387/101‑1 (MQ=255)
                                                       gCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCa                                               <  2:4579731/101‑1 (MQ=255)
                                                       gCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCa                                               <  2:3519349/101‑1 (MQ=255)
                                                           gAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATg                                           >  1:8459061/1‑101 (MQ=255)
                                                           gAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATg                                           >  1:3400442/1‑101 (MQ=255)
                                                            aaGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGt                                          >  2:4732222/1‑101 (MQ=255)
                                                             aGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTc                                         <  1:2242787/101‑1 (MQ=255)
                                                              gACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTcc                                        <  1:8897933/101‑1 (MQ=255)
                                                              gACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGACTGTTTTATCTTGCCTCAAATGTcc                                        >  2:5128955/1‑101 (MQ=255)
                                                                 gAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCgg                                     <  1:764536/101‑1 (MQ=255)
                                                                 gAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCgg                                     >  1:5259288/1‑101 (MQ=255)
                                                                     tAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGc                                 >  2:8623024/1‑101 (MQ=255)
                                                                     tAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGc                                 >  1:80809/1‑101 (MQ=255)
                                                                     tAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGc                                 >  2:3088394/1‑101 (MQ=255)
                                                                     tAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGAGc                                 >  2:6927936/1‑101 (MQ=255)
                                                                     gggcaGGGGGCGCTGCCCCCTCTTGGCCTTTGGCCAATTCACCCCTGGGATATTTCGGGCAAGATGAAATCATGCCCCTTCATCTTGCCGCAAATATCCCg                                      >  2:1847686/5‑101 (MQ=0)
                                                                      aaaaGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGcc                                >  2:7271401/1‑101 (MQ=255)
                                                                      aaaaGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGcc                                >  2:5760540/1‑101 (MQ=255)
                                                                       aaaGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCt                               >  1:5390978/1‑101 (MQ=255)
                                                                       aaaGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCt                               >  1:4857215/1‑101 (MQ=255)
                                                                          gggggCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTggg                            <  2:7408813/101‑1 (MQ=255)
                                                                             ggCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGc                         >  2:4860110/1‑101 (MQ=255)
                                                                                gcTGCCCCCTC.TGGC..TTCGCT.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAgc                      <  1:5623611/101‑1 (MQ=255)
                                                                                gcTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAgc                      >  2:8497035/1‑101 (MQ=255)
                                                                                   gCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGcc                   >  2:424602/1‑101 (MQ=255)
                                                                                   gCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGcc                   >  1:6292996/1‑101 (MQ=255)
                                                                                   gCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGcc                   >  1:4233406/1‑101 (MQ=255)
                                                                                    cccccTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGccc                  <  1:7421360/101‑1 (MQ=255)
                                                                                     ccccTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGcccc                 >  2:7481533/1‑101 (MQ=255)
                                                                                      cccTC.TGGC..TTCACC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGccccc                <  1:9120497/101‑1 (MQ=255)
                                                                                       ccTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCa               <  2:6997421/101‑1 (MQ=255)
                                                                                        ctc.tGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAg              >  1:8556061/1‑101 (MQ=255)
                                                                                         tc.tGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGc             <  1:7520559/101‑1 (MQ=255)
                                                                                          c.tGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGcc            >  2:6089892/1‑101 (MQ=255)
                                                                                          c.tGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGcc            >  1:1783020/1‑101 (MQ=255)
                                                                                          c.tGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGcc            <  1:5912113/101‑1 (MQ=255)
                                                                                            tGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCt           >  2:3619558/1‑101 (MQ=255)
                                                                                            tGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCt           <  2:8767309/101‑1 (MQ=255)
                                                                                             ggC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCNCGGGGGCCTGGGGGCAGCGCCCCCGGCCtt          >  1:2816173/1‑101 (MQ=255)
                                                                                              gC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTa         >  2:7845749/1‑101 (MQ=255)
                                                                                                  ttCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGc       <  1:7280417/101‑1 (MQ=255)
                                                                                                    cGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGt     >  1:1401300/1‑101 (MQ=255)
                                                                                                      cc.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGttt   >  1:7925889/1‑101 (MQ=255)
                                                                                                       c.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGTTTc  <  1:4527152/101‑1 (MQ=255)
                                                                                                       c.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGTTTc  <  2:275/101‑1 (MQ=255)
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GATCTGATCGGGGCCGGGCATATCGACACCGATCCGGCGAAAATCGCGGCGTTTCGCCCGAAGACGAAGTAAAAGGGGGCGCTGCCCCCTC.TGGC..TTCGCC.ATTCACCCCCGGGATATTTGAGGCAAGAT.AAAGGAGCCTGTTTTATCTTGCCTCAAATGTCCCGGGGGCCTGGGGGCAGCGCCCCCAGCCTTAGCGTTTC  >  NC_014034/243500‑243700

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG