Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,472,573 +G 8.4% intergenic (‑158/+25) RCAP_rcc02296 ← / ← RCAP_rcc02297 GTP‑binding protein Obg/CgtA/GNAT family acetyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,472,5730.→G 8.4% 67.2 0.0 ‑1.3263intergenic (‑158/+25)RCAP_rcc02296/RCAP_rcc02297GTP‑binding protein Obg/CgtA/GNAT family acetyltransferase
Reads supporting (aligned to +/- strand):  new base (20/2):  ref base (217/24):  total (237/26)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.17e-02

Only 100 of 294 total aligned reads displayed.

TGGGGGCTCTGCCCCCAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGGGAAGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGATCGCATCGAGC  >  NC_014034/2472479‑2472670
                                                                                                                                                                                                
 gggggCTCTGCCCCCAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAgcg                                                                                          <  2:4792062/101‑1 (MQ=255)
  ggggCTCTGCCCCCAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAgcgc                                                                                         >  2:1904124/1‑101 (MQ=255)
       tctGCCCCCAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTcc                                                                                    <  1:756462/101‑1 (MQ=255)
       tctGCCCCCAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTcc                                                                                    >  2:3154833/1‑101 (MQ=255)
            ccccAACCCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGcc                                                                               <  2:6519349/101‑1 (MQ=255)
             cccAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGccc                                                                              <  2:7615190/101‑1 (MQ=255)
             cccAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGccc                                                                              <  2:2268158/101‑1 (MQ=255)
              ccAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCt                                                                             >  2:9130091/1‑101 (MQ=255)
               cAACCCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATTCCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCtt                                                                            <  1:8967075/101‑1 (MQ=37)
                aaaCCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTg                                                                           >  1:3011686/1‑101 (MQ=255)
                 aaCCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGt                                                                          >  1:1909284/1‑101 (MQ=255)
                  aCCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTc                                                                         >  1:5828882/1‑101 (MQ=255)
                    ccccGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAg                                                                       >  1:6721089/1‑101 (MQ=255)
                            tatTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCa                                                               >  2:5250565/1‑101 (MQ=255)
                             tttggcagaaAGAGGAGGGGCCGTGTTTTCTTCTT.CCAAAAATTCCGCGGGGGGGGGT.GGGGGGGGGGCCCCCCCCCCCCCCCCCTTGTCAGCCTGCGCAg                                                              <  1:9126940/97‑1 (MQ=255)
                             atTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAg                                                              >  2:5122202/1‑101 (MQ=255)
                              ttggcagaaAGATGAGGGGCCGTTCTTTCTTTTTCCCTAAAAT.CCCCGGGGGGGGGT.GGGGGGGGGGCGCCCCCCCCCCCCCCCTTGTCAGCCTGCGCAgg                                                             <  1:8475211/98‑1 (MQ=255)
                               ttGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAggc                                                            >  2:2735943/1‑101 (MQ=255)
                                tGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAggcg                                                           >  1:5468886/1‑101 (MQ=255)
                                tGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAggcg                                                           >  2:6073027/1‑101 (MQ=255)
                                tGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAggcg                                                           >  2:543097/1‑101 (MQ=255)
                                 gcagcaAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAggcgg                                                          >  1:8701168/1‑101 (MQ=255)
                                   agcaAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGgcggcg                                                        >  2:3613230/1‑101 (MQ=255)
                                     caAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTc                                                      >  1:5355967/1‑101 (MQ=255)
                                      aaGATGAGGGGCCTTGTTTTCTTCTTGCCTCAAAT.GCCCGGGGGGGGGT.GGGGGGGGGGCGGCCCCCCCCCCCCCCTTGTCAGCCTGCGCAGGCGGCGTCa                                                     <  1:3346448/101‑1 (MQ=255)
                                      aaGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCa                                                     >  1:8315569/1‑101 (MQ=255)
                                      aaGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCa                                                     >  1:4272007/1‑101 (MQ=255)
                                      aaGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGAGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCa                                                     >  2:6267128/1‑101 (MQ=255)
                                      aaGAGGGAGGGCGGTGTTTTCCTCTTCCCTCAAATCTCCGGGGGGGGGT..GGGGGGAGGGCCCCCCCCCCCCCCCCCTTGTCAGCCTGCGCAGGCGGCGTCa                                                     <  1:3479967/101‑1 (MQ=255)
                                       aGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCaa                                                    >  1:7468544/1‑101 (MQ=255)
                                        gATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCaaa                                                   >  2:5750333/1‑101 (MQ=255)
                                        gATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCaaa                                                   >  2:547352/1‑101 (MQ=255)
                                         aTGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAg                                                  >  1:1717793/1‑101 (MQ=255)
                                         aTGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAg                                                  >  2:2328285/1‑101 (MQ=255)
                                          tGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCaaaag                                                 >  2:2798041/1‑99 (MQ=255)
                                          tGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAgg                                                 >  1:4101378/1‑101 (MQ=255)
                                           gAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAggc                                                >  2:2050704/1‑101 (MQ=255)
                                           gAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAggc                                                >  1:5615921/1‑101 (MQ=255)
                                           gAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGACCTTGTCAGCCTGCGCAGGCGGCGTCAAAggc                                                >  1:4678661/1‑101 (MQ=255)
                                            aggggCCGTTTTTTCTTCTTCCCTAAATTCCCCGGGGTGGGGT..GGGGGGGGGGCCCCCCCCCCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAggcg                                               <  1:6489268/99‑1 (MQ=255)
                                            aaGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAggcg                                               >  1:2413073/1‑101 (MQ=255)
                                            aaGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAggcg                                               >  2:6837118/1‑101 (MQ=255)
                                            aaGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAggcg                                               >  2:2490588/1‑101 (MQ=255)
                                             aGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAggcgg                                              >  2:6607730/1‑101 (MQ=255)
                                                gCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGtcggcgc                                           >  1:2261305/1‑101 (MQ=255)
                                                 ccGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGgcggcgcg                                          >  1:1148352/1‑101 (MQ=255)
                                                 ccGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGgcggcgcg                                          >  2:6477210/1‑101 (MQ=255)
                                                  cGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGgcggcgcgg                                         >  1:6408597/1‑101 (MQ=255)
                                                  cGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGgcggcgcgg                                         >  1:2091070/1‑101 (MQ=255)
                                                  cGTGCTTCCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGgcggcgcgg                                         >  2:5913274/1‑101 (MQ=255)
                                                   gTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGgcggcgcggc                                        >  2:1752706/1‑101 (MQ=255)
                                                   gTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGgcggcgcggc                                        >  1:7894600/1‑101 (MQ=255)
                                                   gTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGgcggcgcggc                                        >  1:3120505/1‑101 (MQ=255)
                                                   gTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGgcggcgcggc                                        >  2:7140847/1‑101 (MQ=255)
                                                     gCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCtc                                      >  1:7378778/1‑101 (MQ=255)
                                                      cttccctCTCGCCTCAAATCGCCGGGGGGGGGT..GGGGGGGGGCCGGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCtca                                     <  2:8115777/95‑1 (MQ=255)
                                                       tttCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCtcag                                    >  1:2170905/1‑101 (MQ=255)
                                                       tttCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCtcag                                    >  1:355123/1‑101 (MQ=255)
                                                       tttCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCtcag                                    >  1:5012020/1‑101 (MQ=255)
                                                       tttCATCTTGCCTCAAATATCCCGGGCGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCtcag                                    >  1:1545209/1‑101 (MQ=255)
                                                       tttCATCTTGCCTCAAATATCCCGGGCGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCtcag                                    >  2:6380295/1‑101 (MQ=255)
                                                        ttCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCtcagt                                   >  2:7410919/1‑101 (MQ=255)
                                                          cATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCtcagttt                                 >  2:3106718/1‑101 (MQ=255)
                                                           tcattttcccAAAAAGCCCGGGGGGGGGGGGGGGGGGGGGCCGCCCCCCCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCtcagtt                                  <  1:1419997/92‑1 (MQ=255)
                                                           aTCTTGCCTCAAATATCCCGGGTGGTGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGACGGCGCGGCtcagtttc                                <  2:344576/101‑1 (MQ=255)
                                                           aTCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCtcagtttc                                >  2:1419884/1‑101 (MQ=255)
                                                           aTCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCtcagtttc                                >  2:717112/1‑101 (MQ=255)
                                                            tCTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCtcagtttca                               >  1:5126054/1‑101 (MQ=255)
                                                            tCTTGCCTCAAATATCCCGGGTGGGGCTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCtcagtttca                               >  2:4142349/1‑101 (MQ=255)
                                                             cTTGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCtcagtttcag                              >  1:258590/1‑101 (MQ=255)
                                                              ttGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCtcagtttcagt                             >  1:6351152/1‑101 (MQ=255)
                                                              ttGCCTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCtcagtttcagt                             >  1:1079612/1‑101 (MQ=255)
                                                                 ccTCAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTcc                          >  1:7679143/1‑101 (MQ=255)
                                                                    cAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCTCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCCGTTCcaca                       >  2:8438770/1‑101 (MQ=255)
                                                                    cAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCcaca                       >  1:7087436/1‑101 (MQ=255)
                                                                    cAAATATCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCcaca                       >  1:3621081/1‑101 (MQ=255)
                                                                       atatCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGc                    >  1:4418418/1‑101 (MQ=255)
                                                                       atatCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGc                    >  1:384161/1‑101 (MQ=255)
                                                                       aatcgccGGGGGGGGGT.GGGGGGGGGCCCGCCCCCCCCCCCCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATg                     <  1:4846029/96‑1 (MQ=255)
                                                                        tatCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCCGTTCCACATGCg                   >  1:7970651/1‑101 (MQ=255)
                                                                        tatCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCg                   >  2:3699542/1‑101 (MQ=255)
                                                                         atCCCGCGGGTGGGTTGGGGGGGGGGCCCCCCCCCCCCCCCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGc                    <  2:6963460/101‑1 (MQ=255)
                                                                          tCCCGGGTGGGGTTCGGGG..AGGGCAGCGCCCTCGCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGc                 >  1:4784643/1‑101 (MQ=255)
                                                                              gggTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGa             >  1:8234912/1‑101 (MQ=255)
                                                                               gggggtgggcGGGGGCGGCGCTGCCCCCCCCCCCCCCTTGTCAGCCTGCGCAGGCGGCGGCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAgg              <  2:3797359/92‑1 (MQ=255)
                                                                               ggTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGAt            >  2:8588554/1‑101 (MQ=255)
                                                                               ggTGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGAt            >  1:994340/1‑101 (MQ=255)
                                                                                 tGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGAtgg          >  2:3301404/1‑99 (MQ=255)
                                                                                 tGGGGTTCGGGG..AGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGATCg          >  2:7799490/1‑101 (MQ=255)
                                                                                      ggttcgggGAGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGATCGCAt       >  2:4943531/6‑101 (MQ=255)
                                                                                       gttcgggGAGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGATCGCATc      >  1:6120713/5‑101 (MQ=255)
                                                                                       gttcgggGAGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGATCGCATc      >  1:8869759/5‑101 (MQ=255)
                                                                                          cgggGAGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGATCGCATcgag   >  1:1034992/2‑101 (MQ=255)
                                                                                           gggGAGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGGGGCGCGGCTCAGGCTCAGTTCCACATGCGGCAGGATTCGATcgagc  >  1:540053/1‑101 (MQ=255)
                                                                                           gggGAGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGGTCGCAGcgggc  >  1:2938368/1‑101 (MQ=255)
                                                                                           gggGAGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGATCGCATcgagc  >  1:3892290/1‑101 (MQ=255)
                                                                                           gggGAGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGATCGCATcgagc  >  1:525058/1‑101 (MQ=255)
                                                                                           gggGAGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGATCGCATcgagc  >  2:8022971/1‑101 (MQ=255)
                                                                                           gggGAGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGATCGCATcgagc  >  1:8401643/1‑101 (MQ=255)
                                                                                           gggGAGGGCAGCGCCCTCCCAGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGAGCGCATcgagc  >  2:4273825/1‑101 (MQ=255)
                                                                                                                                                                                                
TGGGGGCTCTGCCCCCAAACCCCCGGGATATTTGCAGCAAGATGAAGGGCCGTGCTTTCATCTTGCCTCAAATATCCCGGGTGGGGTTCGGGGGAAGGGCAGCGCCCTCCCCGCCCTTGTCAGCCTGCGCAGGCGGCGTCAAAGGCGGCGCGGCTCAGTTTCAGTTCCACATGCGGCAGGATCGCATCGAGC  >  NC_014034/2472479‑2472670

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG