Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,486,428 +G 98.9% intergenic (‑56/+138) RCAP_rcc02312 ← / ← soxG hypothetical protein/sarcosine oxidase subunit gamma

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,486,4281.→G98.9% 5.3 ‑0.4 ‑0.3475intergenic (‑56/+138)RCAP_rcc02312/soxGhypothetical protein/sarcosine oxidase subunit gamma
Reads supporting (aligned to +/- strand):  new base (214/256):  ref base (3/2):  total (217/258)
Fisher's exact test strand distribution p-value = 6.64e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.53e-01

Only 100 of 560 total aligned reads displayed.

ATTGTTCTTGTCGGGCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAA.GGG.GCGCTGCCCCTCTCTTG.CCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATCCCGG  >  NC_014034/2486328‑2486525
                                                                                                     |                                                                                                   
aTTGTTCTTGTCGGGCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGaaaa                                                                                                      <  1:7003734/101‑1 (MQ=255)
 ttGTTCTTGTCGGGCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAA.g                                                                                                    <  1:3476699/101‑1 (MQ=255)
  tGTTCTTGTCGGGCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAA.gg                                                                                                   >  1:4899598/1‑101 (MQ=255)
      cTTGTCGGGCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAA.ggg.ggc                                                                                              >  1:6582150/1‑99 (MQ=255)
      cTTGTCGGGCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAA.ggg.ggc                                                                                              <  2:2120343/101‑3 (MQ=255)
        tGTCGGGCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAA.ggg.ggcgc                                                                                            >  2:8422298/1‑97 (MQ=255)
         gTCGGGCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAA.ggg.ggcgct                                                                                           >  1:517415/1‑96 (MQ=255)
         gTCGGGCCGCGCGCCCCCGGATTTCGAATAGCCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAA.ggg.ggggct                                                                                           >  1:6163663/1‑96 (MQ=255)
            gggCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGcc                                                                                         <  1:5040926/101‑1 (MQ=255)
              gCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGcccc                                                                                       <  2:8122407/101‑1 (MQ=255)
                cgcgggCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGccccct                                                                                     <  1:7115197/101‑3 (MQ=255)
                cgcgcgCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGccccct                                                                                     <  1:5345590/101‑3 (MQ=255)
                 gcgcgcCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGccccctc                                                                                    >  1:9157566/1‑98 (MQ=255)
                  cgcgcCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGccccctct                                                                                   <  1:7499773/101‑5 (MQ=255)
                   gcgcCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCtt                                                                                 <  1:7264325/101‑1 (MQ=255)
                   gcgcCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCtt                                                                                 >  2:8557471/1‑101 (MQ=255)
                   gcgcCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCtt                                                                                 >  1:2658829/1‑101 (MQ=255)
                     gcCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTtg.c                                                                              <  1:8624758/101‑1 (MQ=255)
                      cccccGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTtg.cc                                                                             <  2:1643284/101‑1 (MQ=255)
                        cccGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTtg.ccct                                                                           <  2:7941436/101‑3 (MQ=255)
                         ccGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTtg.ccctg                                                                          <  2:6673977/101‑4 (MQ=255)
                           ggATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTtg.ccctgcc                                                                        >  2:7313118/1‑96 (MQ=255)
                           ggATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTtg.ccctgcc                                                                        >  2:4480380/1‑96 (MQ=255)
                            gATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCg                                                                        <  1:8878646/101‑1 (MQ=255)
                            gATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCg                                                                        >  1:6345304/1‑101 (MQ=255)
                                  gAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAg                                                                  >  2:2908323/1‑101 (MQ=38)
                                  gAATAGTCCATGCTGGATCTCCTTAACCTTCGACTTGCGGCAACCCCGGCGGCAAGGCAAGCGAAAAGGGG.GCCCTG.CCCGCTCTTGCCCTGCCGGGCacg                                                                  >  2:3945080/1‑99 (MQ=255)
                                   aaTAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGt                                                                 >  2:7088420/1‑101 (MQ=38)
                                   aaTAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGt                                                                 <  1:1885200/101‑1 (MQ=38)
                                    aTAGTCCATGCTGGATCTCTTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTc                                                                <  2:433451/101‑1 (MQ=38)
                                    aTAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTc                                                                <  1:8756810/101‑1 (MQ=38)
                                     tAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTcc                                                               >  2:3753327/1‑100 (MQ=38)
                                     tAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCa                                                               >  2:542723/1‑101 (MQ=38)
                                     tAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCa                                                               <  2:1135482/101‑1 (MQ=38)
                                         ccATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCAcccc                                                           <  1:2275031/101‑1 (MQ=37)
                                          cATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCAccccc                                                          <  2:2575935/101‑1 (MQ=37)
                                           aTGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCTGGGCAAGTCACCCCCg                                                         <  1:1792274/101‑1 (MQ=37)
                                           aTGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCg                                                         >  2:7163470/1‑101 (MQ=37)
                                           aTGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAA.GGGTGCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCg                                                         >  1:1584823/1‑101 (MQ=38)
                                            tGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCgg                                                        >  1:7717594/1‑101 (MQ=37)
                                                ggATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGata                                                    <  2:6495308/101‑1 (MQ=37)
                                                 gATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGatat                                                   >  2:7821904/1‑101 (MQ=37)
                                                  aTCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATAtt                                                  >  2:1528659/1‑101 (MQ=37)
                                                  aTCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATAtt                                                  <  2:1828994/101‑1 (MQ=37)
                                                  aTCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATAtt                                                  <  2:3318163/101‑1 (MQ=37)
                                                  aTCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATAtt                                                  <  2:8821155/101‑1 (MQ=37)
                                                    ctcCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCa                                                >  2:1350857/1‑101 (MQ=37)
                                                    ctcCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCa                                                >  2:3905768/1‑101 (MQ=37)
                                                    ctcCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCa                                                >  1:8186887/1‑101 (MQ=37)
                                                     tcCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCAt                                               >  2:2774097/1‑101 (MQ=37)
                                                     tcCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCAt                                               <  2:3051316/101‑1 (MQ=37)
                                                      ccTTTGCCTTCGGTCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGGTATTCATg                                              >  2:3528153/1‑101 (MQ=25)
                                                      ccTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATg                                              >  1:599402/1‑101 (MQ=37)
                                                       cTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATgg                                             <  1:7358821/101‑1 (MQ=37)
                                                        tttGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGc                                            >  1:1309584/1‑101 (MQ=37)
                                                        tttGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGc                                            >  1:1636475/1‑101 (MQ=37)
                                                           gCCTTCGATCTGCGGCAAGCCCGGCCGCTAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAg                                         <  1:7964355/101‑1 (MQ=25)
                                                            ccgccGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCTCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGa                                        <  2:4130120/97‑1 (MQ=37)
                                                            ccTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGa                                        >  2:3124639/1‑101 (MQ=37)
                                                               tCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGa                                     <  1:1992307/101‑1 (MQ=37)
                                                                 gATCTGCGGCAAGCCCGGCAGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAag                                   >  2:5759300/1‑101 (MQ=25)
                                                                  aTCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAagg                                  >  2:6320699/1‑101 (MQ=37)
                                                                  aTCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAagg                                  <  1:9039153/101‑1 (MQ=37)
                                                                      gCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGc                              <  1:6895977/101‑1 (MQ=37)
                                                                       cGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCg                             >  2:7442996/1‑101 (MQ=25)
                                                                       cGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCg                             <  2:9090783/101‑1 (MQ=37)
                                                                       cGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCg                             >  1:3071711/1‑101 (MQ=25)
                                                                        ggCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCgt                            <  2:3462805/101‑1 (MQ=37)
                                                                        ggCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCgt                            <  1:5512492/101‑1 (MQ=37)
                                                                        ggCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCgt                            <  2:6251735/101‑1 (MQ=37)
                                                                        ggCAAGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCgg                            >  2:826151/1‑100 (MQ=25)
                                                                           aaGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGtt                         >  1:4092096/1‑101 (MQ=25)
                                                                           aaGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGtt                         <  2:5978372/101‑1 (MQ=37)
                                                                            aGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGttt                        <  1:1023667/101‑1 (MQ=37)
                                                                            aGCCCGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGttt                        <  2:5609926/101‑1 (MQ=37)
                                                                              cccGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCa                      <  2:752987/101‑1 (MQ=37)
                                                                               ccGGCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCAt                     <  2:6977349/101‑1 (MQ=25)
                                                                                 ggCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCt                   >  2:5895066/1‑101 (MQ=25)
                                                                                 ggCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCt                   <  1:5893924/101‑1 (MQ=37)
                                                                                 ggCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCt                   <  2:5102043/101‑1 (MQ=37)
                                                                                 ggCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCt                   <  1:3300765/101‑1 (MQ=37)
                                                                                 ggCCGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTAATCt                   <  1:6753337/101‑1 (MQ=25)
                                                                                   ccGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTg                 >  1:8410909/1‑101 (MQ=25)
                                                                                   ccGCAAGGCAAGCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTg                 <  1:3906121/101‑1 (MQ=25)
                                                                                      caaggcaagCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCa              >  1:4168135/1‑101 (MQ=21)
                                                                                       aaggcaagCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCAt             >  1:2521383/1‑101 (MQ=21)
                                                                                       aaggcaagCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCAt             <  2:8861594/101‑1 (MQ=25)
                                                                                       aaggcaagCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCAt             >  2:2441625/1‑101 (MQ=21)
                                                                                        aggcaagCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATa            >  2:6102838/1‑101 (MQ=21)
                                                                                         ggcaagCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATaa           <  1:454448/101‑1 (MQ=25)
                                                                                         ggcaagCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATaa           <  1:2908421/101‑1 (MQ=25)
                                                                                          gcaagCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATaaa          >  1:3725866/1‑101 (MQ=21)
                                                                                          gcaagCGAAAAGGGG.GCCCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATaaa          <  2:8048241/101‑1 (MQ=21)
                                                                                           caagCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAat         >  1:5637781/1‑101 (MQ=21)
                                                                                            aagCGAAAAGGGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAata        >  2:7718688/1‑101 (MQ=21)
                                                                                             agCGAAAAGGGG.GCTCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAatat       <  2:4848879/101‑1 (MQ=21)
                                                                                               gaaaag.GGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATccc    <  2:4700515/99‑1 (MQ=21)
                                                                                                aaaag.GGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATCCCg   <  1:2774179/100‑1 (MQ=21)
                                                                                                 aaag.GGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATCCCgg  >  1:4366199/1‑101 (MQ=21)
                                                                                                 aaag.GGG.GCGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATCCCgg  >  1:4655274/1‑101 (MQ=21)
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ATTGTTCTTGTCGGGCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAA.GGG.GCGCTGCCCCTCTCTTG.CCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATCCCGG  >  NC_014034/2486328‑2486525

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG