Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,486,431 +T 0.6% intergenic (‑59/+135) RCAP_rcc02312 ← / ← soxG hypothetical protein/sarcosine oxidase subunit gamma

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,486,4311.→T 0.6% 2.0 0.0 ‑0.0504intergenic (‑59/+135)RCAP_rcc02312/soxGhypothetical protein/sarcosine oxidase subunit gamma
Reads supporting (aligned to +/- strand):  new base (1/2):  ref base (231/270):  total (232/272)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 576 total aligned reads displayed.

GTTCTTGTCGGGCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAA.G.GGG.CGCTGCCCCTCTCTTG.CCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATCCCGGGGGGAG  >  NC_014034/2486331‑2486531
                                                                                                                                                                                                             
gTTCTTGTCGGGCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAA.g.gg                                                                                                        >  2:1622424/1‑101 (MQ=255)
gTTCTTGTCGGGCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAA.g.gg                                                                                                        >  1:3467404/1‑101 (MQ=255)
 tgcTTGTCGGGCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAA.g.ggg                                                                                                       <  2:5968584/99‑1 (MQ=255)
  tCTTGTCGGGCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAA.g.gggg                                                                                                      >  1:982568/1‑100 (MQ=255)
     tGTCGGGCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAA.g.ggggcgc                                                                                                   >  2:8422298/1‑97 (MQ=255)
      gTCGGGCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAA.g.ggggcgct                                                                                                  >  2:6975768/1‑96 (MQ=255)
        cGGGCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGc                                                                                                >  1:6834270/1‑101 (MQ=255)
        cGGGCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAA.GCGGG.CGCTGc                                                                                                >  1:2651482/1‑101 (MQ=255)
         gggCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGcc                                                                                               <  1:8576844/101‑1 (MQ=255)
          ggCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGccc                                                                                              >  2:5094345/1‑101 (MQ=255)
           gCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGcccc                                                                                             <  2:8122407/101‑1 (MQ=255)
            ccccccgcCCCCGTATTTCGAATCGTCCATGCTGGATCTCCTTTGCCTTCGATCTCCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGccccc                                                                                            <  1:4296328/96‑2 (MQ=255)
             cgcgggCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGccccct                                                                                           <  1:7115197/101‑3 (MQ=255)
             cgcgcgCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGccccct                                                                                           <  1:3993637/101‑3 (MQ=255)
              gcgcgcCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGccccctc                                                                                          <  1:5245191/101‑4 (MQ=255)
                gcgcCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCtt                                                                                       <  1:7264325/101‑1 (MQ=255)
                gcgcCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCtt                                                                                       >  2:8557471/1‑101 (MQ=255)
                  gcCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTtg.c                                                                                    >  2:1302102/1‑101 (MQ=255)
                    ccccGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTtg.ccc                                                                                  <  1:770498/101‑2 (MQ=255)
                     cccGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTtg.ccct                                                                                 <  2:7941436/101‑3 (MQ=255)
                       cGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTtg.ccctgc                                                                               <  2:1770456/101‑5 (MQ=255)
                        ggATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTtg.ccctgcc                                                                              >  2:4480380/1‑96 (MQ=255)
                        ggATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTtg.ccctgcc                                                                              >  2:5445047/1‑96 (MQ=255)
                        ggATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTtg.ccctgcc                                                                              >  2:5748074/1‑96 (MQ=255)
                         gATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCg                                                                              >  2:8792724/1‑101 (MQ=255)
                          aTTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCgg                                                                             >  2:8035007/1‑101 (MQ=255)
                           tttCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCggg                                                                            >  1:1699085/1‑101 (MQ=255)
                            ttCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTtg.ccctgccagat                                                                          >  1:4144246/1‑92 (MQ=255)
                            ttCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGc                                                                           <  1:6344957/101‑1 (MQ=38)
                              cGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCaa                                                                         <  1:7500907/101‑1 (MQ=38)
                               gAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAg                                                                        >  1:225310/1‑101 (MQ=38)
                               gAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAg                                                                        <  2:2548320/101‑1 (MQ=38)
                               gAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAg                                                                        >  2:2908323/1‑101 (MQ=38)
                                 aTAGTCCATGCTGGATCTCTTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTc                                                                      <  2:433451/101‑1 (MQ=38)
                                 aTAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCGGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTc                                                                      >  1:5005390/1‑101 (MQ=38)
                                  tAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTcc                                                                     >  1:6720615/1‑100 (MQ=38)
                                  tAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTcc                                                                     >  2:3753327/1‑100 (MQ=38)
                                   aGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCAc                                                                    >  2:9120841/1‑101 (MQ=38)
                                   aGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCAc                                                                    <  1:3895149/101‑1 (MQ=38)
                                    gTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTGAcc                                                                   >  2:7312371/1‑101 (MQ=38)
                                    gTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCAcc                                                                   <  1:4525440/101‑1 (MQ=38)
                                    ctCCATGCTGCATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCAcc                                                                   <  1:7359761/100‑1 (MQ=37)
                                       cATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCAccccc                                                                <  1:6447719/101‑1 (MQ=37)
                                        aTGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCg                                                               <  2:6311662/101‑1 (MQ=37)
                                            tGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGat                                                           >  1:1265085/1‑101 (MQ=37)
                                               aTCTCCTTTGCCTTCGATCTGCGGCAAGCTTGGCCGCAAGGCAAGCGAAAA.G.GGGCCGCTGCCCC.CTCTTGCCCTGGCGGGCAGGTCCCGCCCGGGCTAtt                                                        >  2:7438330/1‑101 (MQ=255)
                                               aTCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATAtt                                                        <  2:3318163/101‑1 (MQ=37)
                                                tctcCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTc                                                       <  1:2758742/101‑1 (MQ=37)
                                                 ctcCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCa                                                      >  2:3905768/1‑101 (MQ=37)
                                                 ctcCTTGGCCTGCGAGCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCa                                                      <  2:1952518/101‑1 (MQ=25)
                                                   ccTTTGCCTTCGGTCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGGTATTCATg                                                    >  2:3528153/1‑101 (MQ=25)
                                                   ccTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATg                                                    <  2:5571416/101‑1 (MQ=37)
                                                   ccTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATg                                                    >  1:599402/1‑101 (MQ=37)
                                                      ttGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCa                                                 <  2:2658747/101‑1 (MQ=37)
                                                       tGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCaa                                                >  2:3121355/1‑101 (MQ=37)
                                                       tGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCaa                                                <  1:7982407/101‑1 (MQ=37)
                                                         ccgccGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CTCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGa                                              <  2:4130120/97‑1 (MQ=37)
                                                           ttCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATg                                            >  2:4770240/1‑101 (MQ=37)
                                                           ttCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATg                                            <  1:882622/101‑1 (MQ=37)
                                                            tCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGa                                           >  2:2997453/1‑101 (MQ=37)
                                                            tCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGa                                           <  2:6636808/101‑1 (MQ=37)
                                                             cGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGaa                                          <  1:542733/101‑1 (MQ=37)
                                                              gATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAag                                         >  1:9103308/1‑101 (MQ=37)
                                                              gATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAag                                         >  2:7718496/1‑101 (MQ=37)
                                                               aTCTGCGGCAAGCCCGGCCGTAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAagg                                        <  1:5603848/101‑1 (MQ=25)
                                                               aTCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAagg                                        <  1:1898924/101‑1 (MQ=37)
                                                                    cGGCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCg                                   >  1:3071711/1‑101 (MQ=25)
                                                                     ggCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCgt                                  <  2:3462805/101‑1 (MQ=37)
                                                                     ggCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCgt                                  <  2:6251735/101‑1 (MQ=37)
                                                                     ggCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCgt                                  <  2:7521687/101‑1 (MQ=37)
                                                                     ggCAAGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCgg                                  >  2:826151/1‑100 (MQ=25)
                                                                         aGCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGttt                              <  1:1023667/101‑1 (MQ=37)
                                                                          gCCCGGCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTc                             <  1:1808862/101‑1 (MQ=25)
                                                                              ggCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCt                         <  1:5893924/101‑1 (MQ=37)
                                                                               gCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCtt                        <  1:5153377/101‑1 (MQ=21)
                                                                               gCCGCAAGGCAAGCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGACAAGATGAAGGAGGCGTGTTTCATCtt                        >  2:7816312/1‑101 (MQ=21)
                                                                                  gcaaggcaagCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGcc                     <  2:1449822/101‑1 (MQ=25)
                                                                                  gcaaggcaagCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGcc                     >  1:4610360/1‑101 (MQ=21)
                                                                                   caagccaagCGAAAAGG.GGG.CCCTGCCCC.CTCTTGCCCTGCCGGGCAAGTGACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCa                    <  2:5886056/101‑1 (MQ=21)
                                                                                    aaggcaagCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCAt                   >  1:2521383/1‑101 (MQ=21)
                                                                                    aaggcaagCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCAt                   <  2:8861594/101‑1 (MQ=25)
                                                                                    aaggcaagCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCAt                   >  2:3953688/1‑101 (MQ=21)
                                                                                     aggcaagCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATa                  >  2:6102838/1‑101 (MQ=21)
                                                                                      ggcaagCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATaa                 <  1:2908421/101‑1 (MQ=25)
                                                                                      ggcaagCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATaa                 <  1:3596392/101‑1 (MQ=21)
                                                                                         aagCGAAAAGG.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAata              >  1:1578967/1‑101 (MQ=21)
                                                                                           cgaaaag.G.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATcc           <  1:6976053/98‑1 (MQ=21)
                                                                                            gaaaag.G.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATccc          <  2:7058335/99‑1 (MQ=21)
                                                                                            gaaaag.G.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATccc          >  1:8634884/3‑101 (MQ=21)
                                                                                            gaaaag.G.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATccc          <  2:4700515/99‑1 (MQ=21)
                                                                                             aaaag.G.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATCCCg         >  1:8945427/2‑101 (MQ=18)
                                                                                              aaag.G.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATCCCgg        >  1:8365582/1‑101 (MQ=21)
                                                                                              aaag.G.GGG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATCCCgg        <  2:2356762/101‑1 (MQ=21)
                                                                                                aagg.ggg.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATCCCggg       >  1:206776/4‑101 (MQ=21)
                                                                                                 agg.ggg.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATCCCgggg      >  1:3293634/3‑101 (MQ=18)
                                                                                                  gg.ggg.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATACCggggg     >  2:6458894/2‑101 (MQ=18)
                                                                                                   g.ggg.CGCTG.CCCACTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGCCGTGTTTCATCTTGCCATAAATATCCCgggggg    <  1:4770391/101‑1 (MQ=21)
                                                                                                     ggg.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATCCCGGGGGGa   <  2:7136268/101‑1 (MQ=255)
                                                                                                     ggg.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTCTCATCTTGCCATAAAGATCCCGGGGGGa   <  1:8090738/101‑1 (MQ=21)
                                                                                                      gg.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATCCCGGGGGGAg  <  1:6068962/101‑1 (MQ=255)
                                                                                                                                                                                                             
GTTCTTGTCGGGCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGAAAA.G.GGG.CGCTGCCCCTCTCTTG.CCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATCCCGGGGGGAG  >  NC_014034/2486331‑2486531

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG