Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,056 +T 4.2% intergenic (+104/‑138) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0561.→T 4.2% 39.0 ‑0.6 ‑0.5284intergenic (+104/‑138)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/6):  ref base (162/110):  total (168/116)
Fisher's exact test strand distribution p-value = 5.57e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.23e-01

Only 100 of 309 total aligned reads displayed.

GGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAGAAGGGGGGCAAGCG.C.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGA  >  NC_014034/2493957‑2494152
                                                                                                    |                                                                                                      
gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                                                                       >  2:4682426/1‑101 (MQ=255)
 ggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tg                                                                                                      <  2:3627691/101‑1 (MQ=255)
  gcgcTCCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tgg                                                                                                     <  2:1947132/101‑1 (MQ=255)
   cgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGc                                                                                                    <  1:3525941/101‑1 (MQ=255)
    gcTGCCCCCCGTCCGCAAGCGGACTCCCCGCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..g                                                                                                 <  2:2362552/101‑1 (MQ=255)
    gcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..g                                                                                                 <  1:3322414/101‑1 (MQ=255)
    gcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGCGGGTGAA.TGGC..g                                                                                                 >  2:700788/1‑101 (MQ=255)
     cTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.a                                                                                               <  2:3030375/101‑1 (MQ=255)
      tGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aa                                                                                              <  1:7350725/101‑2 (MQ=255)
       gCCCCCCGTCCGCAAGCGGAGTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGCGGGTGAA.TGGC..G.aag                                                                                             <  1:7251021/101‑3 (MQ=21)
       gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                                                             <  1:984402/101‑3 (MQ=255)
       gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                                                             >  1:4600842/1‑99 (MQ=255)
        cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                                                           <  1:9107171/101‑5 (MQ=255)
        cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                                                           <  1:8102985/101‑5 (MQ=255)
         ccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcca                                                                                          >  2:5877657/1‑96 (MQ=255)
            ccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccaga                                                                                        >  2:3327175/4‑94 (MQ=255)
              gtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagagg                                                                                      <  2:1152574/100‑10 (MQ=255)
               tCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.GGGG..G.aagccagaggg                                                                                     >  1:2373765/1‑91 (MQ=18)
                     aGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGCag                                                                               <  1:6927312/101‑2 (MQ=18)
                         gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.c.c                                                                        >  2:3447109/1‑101 (MQ=18)
                         gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGG.AAGCG.c.c                                                                        >  2:264577/1‑101 (MQ=18)
                          aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.c.cc                                                                       >  1:489283/1‑101 (MQ=18)
                          aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.c.cc                                                                       >  2:8319079/1‑101 (MQ=18)
                           cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.c.ccc                                                                      >  2:759813/1‑101 (MQ=18)
                            tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCGGAGGGGGGC.AGCG.c.cccc                                                                     >  2:1095838/1‑101 (MQ=18)
                             ccccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.c.ccccc                                                                    >  2:5039857/1‑100 (MQ=18)
                              cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.c.ccccct                                                                   <  1:3132562/101‑3 (MQ=17)
                                cccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.c.ccccctgc                                                                 <  2:7704352/101‑5 (MQ=17)
                                cccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.c.ccccctgc                                                                 <  1:560356/101‑5 (MQ=17)
                                  cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGccc                                                                >  2:4432444/1‑101 (MQ=17)
                                  cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGccc                                                                <  2:1626352/101‑1 (MQ=17)
                                  cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.c.cccactgctc                                                               >  2:7215451/1‑94 (MQ=17)
                                   gggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.C.CCCCTGcccc                                                               >  1:4013546/1‑101 (MQ=0)
                                   gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGcccc                                                               <  2:2158618/101‑1 (MQ=17)
                                   gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGcccc                                                               <  2:3818141/101‑1 (MQ=17)
                                    ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.C.CCCCTGCCCcg                                                              >  1:2146677/1‑101 (MQ=2)
                                    ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.C.CCCCCTcccccg                                                             >  2:4275236/1‑99 (MQ=17)
                                      atatTTGCGGCAAGATGAAGGGTCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCAAGAGGGGGCAGCG.C.CCCCTGCCCcgcc                                                            <  2:8953323/101‑3 (MQ=2)
                                      atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCcggc                                                            >  2:1031933/1‑101 (MQ=18)
                                      atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCcggc                                                            >  2:2661722/1‑101 (MQ=17)
                                      atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCcggc                                                            >  2:4744540/1‑101 (MQ=17)
                                       tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCcggcg                                                           >  1:6499480/1‑101 (MQ=18)
                                        atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CAAGAGGGGGGC.AGCGCC.CCCCTGCCCcggcgg                                                          >  1:4507048/1‑101 (MQ=18)
                                         tttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGt                                                         >  2:3144781/1‑101 (MQ=18)
                                          ttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTc                                                        <  1:7429955/101‑1 (MQ=17)
                                          ttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGAGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCcggcggcc                                                        >  1:8007750/1‑99 (MQ=17)
                                           tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTcc                                                       <  2:6445306/101‑1 (MQ=17)
                                            gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCg                                                      >  2:4482595/1‑101 (MQ=18)
                                            gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCg                                                      >  2:1361329/1‑101 (MQ=18)
                                            gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGGCCg                                                      >  2:6821784/1‑101 (MQ=17)
                                            gcagcaAGATGAAACCGGATGCCTTTCACCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCg                                                      <  1:6395668/101‑1 (MQ=17)
                                             cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGt                                                     <  1:1876870/101‑1 (MQ=17)
                                              agcaAGATGAAACCGGATGGCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TCGC..GAAG.CCAGAGGGGGGCAGCGG.C.CCCCTGCCCCGGCGCTCCGTg                                                    >  2:8193392/1‑101 (MQ=17)
                                              agcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTg                                                    <  2:3922043/101‑1 (MQ=17)
                                              agcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTg                                                    >  1:142136/1‑101 (MQ=17)
                                                caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGG.AAGCGCC.CCCCTGCCCCGCCGGACCGTgcc                                                  >  2:3689875/1‑99 (MQ=17)
                                                 aataTGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTggcg                                                 >  1:2602822/4‑101 (MQ=17)
                                                  aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTggcgg                                                >  1:6193330/1‑101 (MQ=17)
                                                  aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTggcgg                                                >  2:257776/1‑101 (MQ=18)
                                                     tGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGAC.CCCCTGCCCCGGCGGTCCGCGGAGgcgc                                             >  1:8960017/1‑101 (MQ=17)
                                                      gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGcc                                            <  2:5453497/101‑1 (MQ=17)
                                                      gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGcc                                            >  2:6677205/1‑101 (MQ=18)
                                                       aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCt                                           <  2:1258087/101‑1 (MQ=17)
                                                       aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCt                                           <  2:2828971/101‑1 (MQ=17)
                                                       aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCG.CGCCCCTGCCCCGCCGGTCCGCGGGGGCCccc                                           >  1:290759/1‑100 (MQ=9)
                                                        aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCtt                                          >  1:4189766/1‑101 (MQ=18)
                                                        aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCtt                                          <  2:5378781/101‑1 (MQ=17)
                                                        aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCtt                                          <  2:4600707/101‑1 (MQ=17)
                                                          ccGGATGCCTTTCATCTTGCCCGAAATGTCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGa                                        <  1:382543/101‑1 (MQ=17)
                                                            ggATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.CGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcg                                      >  1:7676189/1‑101 (MQ=17)
                                                            ggATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcg                                      >  2:5337349/1‑101 (MQ=18)
                                                              aTGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcggg                                    <  2:4910541/101‑1 (MQ=17)
                                                               tGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGGA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggc                                   <  2:847206/101‑1 (MQ=17)
                                                               cgCCTCTAATCTTGCCCGAAATATCCCGGGGGCTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCtttgtcgag                                   <  2:602038/100‑8 (MQ=9)
                                                                    ttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCggggg                              >  2:7456883/1‑101 (MQ=18)
                                                                      cATCTTGCCCGAAATATCCCGGGGGGCGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgcg                            >  2:8551981/1‑101 (MQ=18)
                                                                        tCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGggcg                          >  1:8243437/1‑101 (MQ=18)
                                                                        tCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGcgcg                          >  1:5222058/1‑101 (MQ=18)
                                                                         cTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCgg                         >  1:5063574/1‑101 (MQ=18)
                                                                         cTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCCGGGGGGCgg                         >  1:6718963/1‑101 (MQ=17)
                                                                          ttGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCggg                        >  2:1775279/1‑101 (MQ=18)
                                                                              ccGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt                    <  2:7916273/101‑1 (MQ=18)
                                                                              ccGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt                    <  1:1313960/101‑1 (MQ=18)
                                                                               cGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTa                   <  1:1073244/101‑1 (MQ=18)
                                                                               cGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTa                   <  1:7117265/101‑1 (MQ=18)
                                                                                gAAATATCCGGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaa                  <  2:4154282/101‑1 (MQ=18)
                                                                                 aaaTATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAc                 <  1:8186217/101‑1 (MQ=18)
                                                                                 aaaTATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAc                 >  1:7796682/1‑101 (MQ=18)
                                                                                  aaTATCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAcc                >  2:3265307/1‑101 (MQ=21)
                                                                                    tatCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGa              >  2:9061627/1‑101 (MQ=21)
                                                                                     atCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaa             >  2:4010708/1‑101 (MQ=21)
                                                                                     atCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaa             >  2:2021604/1‑101 (MQ=21)
                                                                                     atCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaa             >  1:866606/1‑101 (MQ=21)
                                                                                      tCCCGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaaa            <  1:5877892/101‑1 (MQ=21)
                                                                                        ccGGGGGGTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAtt          <  2:5608592/101‑1 (MQ=21)
                                                                                           gggggTGAA.TGGC..GAAGCCAG.AGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa       >  1:7524311/1‑101 (MQ=25)
                                                                                           gggggTGAA.TGGC..GAAGCCAG.AGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa       >  1:7724443/1‑101 (MQ=25)
                                                                                              ggTGAA.TGGC..GAAGCCAG.AGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACa    >  1:1176138/1‑101 (MQ=25)
                                                                                              ggTGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACa    <  1:2708145/101‑1 (MQ=25)
                                                                                                tGAA.TGGC..GAAG.CCAGAGGGGGGC.AGCGCC.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGa  >  1:570159/1‑101 (MQ=25)
                                                                                                    |                                                                                                      
GGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAGAAGGGGGGCAAGCG.C.CCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGA  >  NC_014034/2493957‑2494152

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG