Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,566,557 +C 99.0% intergenic (+29/+165) purD → / ← sdaA phosphoribosylamine‑‑glycine ligase/L‑serine ammonia‑lyase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,566,5572.→C99.0% 3.1 ‑1.2 ‑0.2383intergenic (+29/+165)purD/sdaAphosphoribosylamine‑‑glycine ligase/L‑serine ammonia‑lyase
Reads supporting (aligned to +/- strand):  new base (103/276):  ref base (2/2):  total (105/278)
Fisher's exact test strand distribution p-value = 3.03e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.06e-01

Only 100 of 441 total aligned reads displayed.

ATGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAAC.GGG.GGCGCTG..CCCCC.CTC.CTG..CTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTT  >  NC_014034/2566458‑2566649
                                                                                                           |                                                                                             
    gATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGCCATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.ATC.TTggcct                                                                                            <  1:8291003/101‑5 (MQ=255)
     gtGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTggcctt                                                                                           <  2:388138/100‑6 (MQ=38)
      tGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTggccttc                                                                                          <  1:3148110/101‑7 (MQ=38)
        gTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTggccttcgg                                                                                        <  1:5618894/101‑9 (MQ=38)
          cGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGTCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGcc                                                                                      >  1:3686276/1‑101 (MQ=37)
          cGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGcc                                                                                      <  1:6654910/101‑1 (MQ=37)
          cGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGcc                                                                                      >  1:3595548/1‑101 (MQ=37)
           gATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGGGCGG..CCCCC.CTC.TTGGCCTTCGGccc                                                                                     >  1:2942549/1‑100 (MQ=37)
           gATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCa                                                                                     <  2:8917344/101‑1 (MQ=37)
                gATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCa                                                                                <  2:7814776/101‑1 (MQ=37)
                  tCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCAcc                                                                              <  1:193020/101‑1 (MQ=37)
                  tCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCAcc                                                                              <  2:7001388/101‑1 (MQ=37)
                      cTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCAcccccc                                                                          >  2:5871235/1‑101 (MQ=25)
                       tGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCg                                                                         <  1:6541963/101‑1 (MQ=25)
                       tGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCCCTGAGACG.AAACGGGG.GGCGCTGCCCCCCC.CTT.CGG..CTTCCGGCAAATCAccccccc                                                                         >  1:4358873/1‑100 (MQ=255)
                         gCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCCATTCCCCCCCCcgg                                                                       >  2:1389845/1‑101 (MQ=25)
                         gCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCggg                                                                       <  1:4101714/101‑1 (MQ=25)
                           ccGAGGGGTTCTGCCGCCGCGACATCGGCTGGGGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCCGCCACTTCACCCCCCcggcg                                                                     >  2:6115917/1‑99 (MQ=21)
                            cGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGata                                                                    <  2:2617998/101‑1 (MQ=25)
                             gAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCCATTCACCCCCCGGGatat                                                                   >  1:525409/1‑101 (MQ=25)
                             gAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGatat                                                                   <  2:2566587/101‑1 (MQ=25)
                               ggggTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATAttt                                                                 <  1:6705999/101‑1 (MQ=21)
                               ggggTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATAttt                                                                 <  2:848735/101‑1 (MQ=21)
                               ggggTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATAttt                                                                 <  1:1822618/101‑1 (MQ=21)
                               ggggTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATAttt                                                                 <  2:2389610/101‑1 (MQ=21)
                                gggTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTc                                                                <  1:7868198/101‑1 (MQ=21)
                                gggTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTc                                                                <  1:5487580/101‑1 (MQ=21)
                                 ggTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACGAAAAG.GGG.GGGGCTG..CCCCC.CCC.CTGGCCTTCGGCCCACTCACCCCCCGGGCTATTTCg                                                               >  1:4732184/1‑101 (MQ=255)
                                  gTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCgg                                                              <  2:3133003/101‑1 (MQ=21)
                                   ttCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCggg                                                             <  2:3590254/101‑1 (MQ=21)
                                    tCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGc                                                            <  1:5785720/101‑1 (MQ=21)
                                    tCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGc                                                            <  2:3456355/101‑1 (MQ=21)
                                    tCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGc                                                            <  1:6882056/101‑1 (MQ=21)
                                     cTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CCC.TTGGCCCTCGGCCCACTCACCCCCCCGGAGATTTCGGGCa                                                           >  1:7063049/1‑101 (MQ=21)
                                       gccgccGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGGTAATTCGGGCCAg                                                         >  2:4575522/1‑101 (MQ=21)
                                       gccgccGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAg                                                         <  1:3981766/101‑1 (MQ=21)
                                         cgccgcGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGAt                                                       <  1:4597723/101‑1 (MQ=21)
                                          gccgcTCCAGCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATa                                                      <  1:1676109/101‑1 (MQ=18)
                                          gccgcGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATa                                                      <  2:714543/101‑1 (MQ=21)
                                          gccgcGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATa                                                      <  2:4008271/101‑1 (MQ=21)
                                          gccgcGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATa                                                      <  2:1018975/101‑1 (MQ=21)
                                           ccgcGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATaa                                                     <  2:3706003/101‑1 (MQ=21)
                                            cgcgACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATaaa                                                    <  1:2321962/101‑1 (MQ=21)
                                            cgcgACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATaaa                                                    <  2:6339159/101‑1 (MQ=21)
                                             gcgACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGGTAaag                                                   >  1:1039313/1‑101 (MQ=21)
                                             gcgACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAaag                                                   <  1:869769/101‑1 (MQ=21)
                                              cgACATCGGCTGGCGCGCGCTCTGAGACG.AACC.GGGTGGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAaaga                                                  <  1:8155435/101‑1 (MQ=21)
                                              cgACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAaaga                                                  >  1:1213652/1‑101 (MQ=21)
                                                aCATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAaagaag                                                <  1:7448058/101‑1 (MQ=21)
                                                 cATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAgg                                               <  2:6521144/101‑1 (MQ=21)
                                                   tCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTc                                             <  2:1474153/101‑1 (MQ=21)
                                                   tCGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTc                                             <  2:6955122/101‑1 (MQ=21)
                                                    cGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCt                                            >  1:2417895/1‑101 (MQ=21)
                                                    cGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCt                                            <  1:2142862/101‑1 (MQ=21)
                                                    cGGCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCt                                            >  1:5101828/1‑101 (MQ=21)
                                                     ggCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCtt                                           <  1:8889796/101‑1 (MQ=21)
                                                     ggCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCtt                                           <  2:5216077/101‑1 (MQ=21)
                                                      gCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCCCCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCttt                                          <  1:1994603/101‑1 (MQ=21)
                                                      gCTGGCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCttt                                          <  2:2225602/101‑1 (MQ=21)
                                                       cTGGCGCGCGCTCTGAGACG.GAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCcttt                                         <  1:7201113/101‑1 (MQ=18)
                                                         ggCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTcc                                       <  1:2649652/101‑1 (MQ=21)
                                                         ggCGCGCGCTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTcc                                       >  2:3295937/1‑101 (MQ=21)
                                                            gcgcgcTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCttt                                    <  1:4913876/101‑1 (MQ=21)
                                                             cgcgcTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTc                                   <  2:9046709/101‑1 (MQ=21)
                                                             cgcgcTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTc                                   <  2:7303657/101‑1 (MQ=21)
                                                             cgcgcTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTc                                   <  2:2198703/101‑1 (MQ=21)
                                                              gcgcTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCa                                  <  2:8246253/101‑1 (MQ=21)
                                                               cgcTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCAt                                 <  2:1856942/101‑1 (MQ=21)
                                                               cgcTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCAt                                 <  1:9139606/101‑1 (MQ=21)
                                                                gcTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATc                                >  1:6396003/1‑101 (MQ=21)
                                                                gcTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATc                                <  1:8201249/101‑1 (MQ=21)
                                                                gcTCTGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATc                                <  2:4961700/101‑1 (MQ=21)
                                                                 ctctGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCt                               >  1:531514/1‑101 (MQ=21)
                                                                 ctctGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCACCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCt                               <  1:9034448/101‑1 (MQ=21)
                                                                  tctGAGACG.AACCGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCtt                              <  2:2925057/101‑1 (MQ=21)
                                                                  tctGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCtt                              >  1:2786357/1‑101 (MQ=21)
                                                                  tctGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCtt                              <  2:5538083/101‑1 (MQ=21)
                                                                  tctGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCtt                              <  2:8056132/101‑1 (MQ=21)
                                                                   ctGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTg                             <  2:3944920/101‑1 (MQ=21)
                                                                   ctGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTg                             >  2:7561907/1‑101 (MQ=21)
                                                                    tGAGACG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGc                            <  2:4347223/101‑1 (MQ=21)
                                                                       gaCG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCTCCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCg                         <  2:1758981/101‑1 (MQ=21)
                                                                       gaCG.AAACGGGG.GGCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCg                         >  2:7884299/1‑101 (MQ=21)
                                                                              gcaggg.ggCGCTG...CCCC.CTC.TTGGCCTTTGGCCAATTCA.CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCggg             >  2:3238347/4‑101 (MQ=0)
                                                                               cgggg.ggCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATc                  >  1:7314422/3‑101 (MQ=25)
                                                                               caggg.ggCGCTG...CCCC.CTC.TTGGCCTTTGGCCAATTCA.CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCgggg            >  2:2476428/3‑101 (MQ=0)
                                                                                gggg.ggCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTTGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTCCCCGAAATATcc                 >  1:6293486/2‑101 (MQ=21)
                                                                                gggg.ggCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATcc                 >  2:2802692/2‑101 (MQ=25)
                                                                                gggg.ggCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATcc                 >  2:5567924/2‑101 (MQ=25)
                                                                                aggg.ggCGCTG..CCCCCTCTT.GGC..CTTTGGCCAATTCA.CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCggggg           >  2:2060547/2‑101 (MQ=0)
                                                                                aggg.ggCGCTG...CCCC.CTC.TTGGCCTTTGGCCAATTCA.CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCggggg           >  1:8508213/2‑101 (MQ=0)
                                                                                 ggg.ggCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATccc                <  1:6099455/101‑1 (MQ=25)
                                                                                  gg.ggCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCg               >  2:465830/1‑101 (MQ=25)
                                                                                   g.ggCGCTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCgg              >  1:327226/1‑101 (MQ=25)
                                                                                     ggCGCTG..CCCCCTCTT.GGC..CTTTGGCCAATTCA.CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCggggggagc       <  2:7410274/101‑5 (MQ=2)
                                                                                       ctcTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCggggg           <  2:6644552/99‑1 (MQ=37)
                                                                                       cgcTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCggggg           >  1:7655364/1‑101 (MQ=37)
                                                                                         cTG..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAg         <  1:1389877/101‑1 (MQ=37)
                                                                                          tG..CCCCC.CTCTCGGC.CTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGc        <  2:870211/101‑1 (MQ=37)
                                                                                           g..CCCCC.CTC.TTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGcc       >  1:8062860/1‑101 (MQ=37)
                                                                                                           |                                                                                             
ATGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAAC.GGG.GGCGCTG..CCCCC.CTC.CTG..CTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTT  >  NC_014034/2566458‑2566649

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG