Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,780,410 +T 1.3% coding (980/1257 nt) RCAP_rcc02591 ← surface presentation of antigens protein family

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,780,4101.→T 1.3% 7.6 ‑2.1 ‑0.1298coding (980/1257 nt)RCAP_rcc02591surface presentation of antigens protein family
Reads supporting (aligned to +/- strand):  new base (4/0):  ref base (145/149):  total (149/149)
Fisher's exact test strand distribution p-value = 1.22e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.03e-01

Only 100 of 322 total aligned reads displayed.

AGACCGCCGCCACTGCCGGGCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTG.GCCCCGCCTCGAAGGGCGGCGGCACGACGCGGCGCACCTTCGGTTCGGGCGGCGCCATCAGCTCTTCTTCCTCGTGTTCGATCAGCCGCACCGCGCG  >  NC_014034/2780311‑2780510
                                                                                                    |                                                                                                     
 gACCGCCGCCACTGCCGGGCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.tt                                                                                                     <  2:4493455/101‑1 (MQ=255)
 gACCGCCGCCACTGCCGGGCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.tt                                                                                                     <  2:1900330/101‑1 (MQ=255)
     gccgccACTGCCGGGCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.tttttc                                                                                                 >  1:3392290/1‑97 (MQ=255)
     gccgccACTGCCGGGCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.tttgtc                                                                                                 >  2:8699728/1‑97 (MQ=255)
      ccgccACTGCCGGGCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.tttgggc                                                                                                >  2:592435/1‑96 (MQ=255)
         ccACTGCCGGGCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTG.GGTCCGc                                                                                            >  1:7474625/1‑101 (MQ=255)
           aCTGCCGGGCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTG.GCCCCGCCt                                                                                          <  2:687383/101‑1 (MQ=255)
           aCTGCCGGGCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTG.GCCCCGCCt                                                                                          <  2:2824738/101‑1 (MQ=255)
            cTGCCGGGCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTG.GCCCCGCCTc                                                                                         <  2:5415743/101‑1 (MQ=255)
            cTGCCGGGCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTG.GCCCCGCCTc                                                                                         <  2:2331817/101‑1 (MQ=255)
             tGCCGGGCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTG.GCCCCGCCTCg                                                                                        <  1:5119318/101‑1 (MQ=255)
             tGCCGGGCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTG.GCCCCGCCTCg                                                                                        <  2:4571703/101‑1 (MQ=255)
              gCCGGGCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTGTGCCCCCccccc                                                                                        >  2:6603002/1‑98 (MQ=255)
                cGGGCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTG.GCCCCGCCTCGAAg                                                                                     <  1:8117698/101‑1 (MQ=255)
                 gggCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTT.GCCCCGcccccgagg                                                                                    >  2:6802844/1‑94 (MQ=255)
                  ggCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTG.GCCCCGCCTCGAAggg                                                                                   <  1:3766984/101‑1 (MQ=255)
                   gCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTT.GCCCCCccccccagggg                                                                                  >  1:2395070/1‑92 (MQ=255)
                   gCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTG.GCCCCGCCTCGAAGggc                                                                                  <  2:482516/101‑1 (MQ=255)
                      gCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCTGCAAAGGGGC.TTG.GCCCCGCCTCGAAGggcggc                                                                               <  1:4787475/101‑1 (MQ=255)
                      gCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTG.GCCCCGCCTCGAAGggcggc                                                                               <  1:6942870/101‑1 (MQ=255)
                       cGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTT.GCCCCGCCCCCCAGgggggcg                                                                              >  1:8704036/1‑101 (MQ=255)
                         gATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTG.GCCCCCCCCCCAAGggcggcggc                                                                            >  1:666051/1‑101 (MQ=255)
                          aTCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTT.GCCCCGCCCCGCAGggaggcggga                                                                           >  2:4216526/1‑99 (MQ=255)
                          aTCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTT.GCCCCCCCCCGCAGgggggggggc                                                                           >  1:5439319/1‑99 (MQ=255)
                          aTCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTG.GCCCCGCCTCGAAGGGCGGCGGCa                                                                           <  1:169646/101‑1 (MQ=255)
                          aTCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTG.GCCCCGCCCCCACGggcgggggcc                                                                           >  1:3633665/1‑100 (MQ=255)
                           tCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTT.GGCCCCCCCCGCAGggggggggggc                                                                          >  1:6830111/1‑98 (MQ=255)
                            cATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTT.GTCCCGCCCCGCAGGGGGGCGGCgcg                                                                         >  1:1581623/1‑101 (MQ=255)
                             aTAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTT.GCCCCCCCCCCCAGGGGGGGGGGacgc                                                                        >  1:2947922/1‑100 (MQ=255)
                             aTAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTG.GCCCCGCCTCGGAGGGCGGCGGCacgg                                                                        >  1:8371100/1‑100 (MQ=255)
                              tAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTT.GGCCCGCCCCCCAGgggggcggggcgag                                                                       >  1:1315565/1‑95 (MQ=255)
                              tAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTG.GCCTCGCCTCGAAGGGCGGCGGCacgac                                                                       <  2:4382549/101‑1 (MQ=255)
                              tAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTG.GCCCCGCCTCGAAGGGCGGGGGCccgac                                                                       >  2:5692793/1‑101 (MQ=255)
                              tAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTG.GCCCCGCCTCGAAGGGCGGCGGCacgac                                                                       <  1:6673928/101‑1 (MQ=255)
                               aGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTT.GGCCCGCCTCGCAGGGCGGCGGCccgacg                                                                      >  2:1108770/1‑101 (MQ=255)
                                 gctggggAAGCGATCCGGTCGAGCGGCGCGCGCGGCCCTCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTG.GCCCCGCCTCGAAGGGCGGCGGCACGAcgcg                                                                    <  2:3570395/98‑1 (MQ=255)
                                 gCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGCTTTG.GCCCCCCCCCGAGGgggggggggcgcagcg                                                                     >  2:2130674/1‑93 (MQ=255)
                                 gCCGGGGAATCGATCCGGTCGAGCGGCGCGCGAGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTG.GCCCCGCCTCGAAGGGCGGCGGCACGAcgcg                                                                    <  2:5017727/101‑1 (MQ=255)
                                 gCCGGGGAAGCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCGAAGGGGC.TTG.GCCCCGCCTCGAAGGGCGGCGGCACGAcgcg                                                                    <  1:4268332/101‑1 (MQ=255)
                                  ccGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTT.GCCCCCCCCCCACGGGGGGGGGCGCGGCgcgg                                                                   >  1:6607892/1‑101 (MQ=255)
                                   cGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTG.GGCCCGCCCCCAAGGGGGGCGGGaccgcgccgc                                                                  >  1:6054199/1‑93 (MQ=255)
                                      ggAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTT.GCCCCGCCCCGAAGGGGGGGGGGCCGACGCGgcgcc                                                               >  1:6280904/1‑100 (MQ=255)
                                        aaTCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTG.GCCCCGCCTCGAAGGGCGGCGGCACGACGCGGCGCAcc                                                             <  1:5534464/101‑1 (MQ=255)
                                          tCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTG.GCCCCGCCTCGAAGGGCGGCGGCACGACGCGGCGCACCtt                                                           <  2:7474301/101‑1 (MQ=255)
                                           cGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTG.GCCCCGCCTCGAAGGGCGGCGGCACGACGCGGCGCACCttc                                                          <  1:8512297/101‑1 (MQ=255)
                                           cGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTG.GCCCCGCCTCGAAGGGCGGCGGCACGACGCGGCGCACCttc                                                          <  2:8240276/101‑1 (MQ=255)
                                            gATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTG.GCCCCGCCCCGCCGGGGGGCGGCCCGACGGGGGGCCCCctcg                                                         >  1:4472542/1‑101 (MQ=255)
                                            caTCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTG.GCCCCGCCTCGAAGGGCGGCGGCACGACGCGGCGCACCttcg                                                         <  2:4282185/100‑1 (MQ=255)
                                               ccGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTG.GCCCCGCCTCGAAGGGCGGCGGCACGACGCGGCGCACCttcggtt                                                      <  2:624728/101‑1 (MQ=255)
                                                   tCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTG.GCCCCGCCTCGAAGGGCGGCGGCACGACGCGGCGCACCTTCGGTTCggg                                                  <  2:5890362/101‑1 (MQ=255)
                                                    cGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTT.GCCCCGCCTCGAAGGGCGGCGGCACGACGCGGCGCACCCTCGGTTCgggg                                                 >  2:3322282/1‑100 (MQ=255)
                                                    cGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTG.GCCCCCCCCCGCAGGGCGGCGGGACGTCGCGGGGCACCCTCGGGTCgggg                                                 >  1:7772820/1‑100 (MQ=255)
                                                    cGAGCCGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTG.GCCCCGCCTCGAAGGGCGGCGGCACGACGCGGCGCACCTTCGGTTCGggc                                                 <  2:4204581/101‑1 (MQ=255)
                                                     gAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTG.GCCCCGCCTCGCAGGGCGGCGGGCCGACGCGGCGCCCCCTCGGTTCGggcg                                                >  1:7270660/1‑101 (MQ=255)
                                                      aGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTT.GGCCCGCCCCGAAGGGGGGCGGCCCGACGCGGGGGACCTTCCGTTTGggcgg                                               >  2:3462580/1‑101 (MQ=255)
                                                        cggcgCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTT.GCCCCGCCTCGAAGGGGGGGGGCNNNNNGCGGGGCCCCCTCCGTTCGGggggcg                                             >  1:5873541/1‑101 (MQ=255)
                                                        cggcgCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTT.GCCCCCCCTCGAAGGGCGGCGGCACGGCGCGGCGCACCCTCGGTTCCggggggg                                             >  1:9017975/1‑99 (MQ=255)
                                                        cggcgCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTG.GCCCCGCCTCGAAGGGCGGCGGCACGACGCGGCGCACCTTCGGTTCGGgcggcg                                             <  2:1494849/101‑1 (MQ=255)
                                                          gcgcgcgcGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTT.GCCCCGCCCCGAAGGGCGGCGGCCCGACGCGGGGCCCCCTCCGTTCGGGCGGGGcc                                           >  1:5712127/1‑101 (MQ=255)
                                                           cgcgcgcgGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTG.GCCCCGCCTCGAAGGGCGGCGGCACGACGCGGCGCACCTTCGGTTCGGGCGGCGCCa                                          <  2:2486264/101‑1 (MQ=255)
                                                           cgcgcgcgGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTG.GCCCCGCCTCGAAGGGCGGCGGCACGACGCGGCGCACCTTCGGTTCGGGCGGCGCCa                                          <  1:8749245/101‑1 (MQ=255)
                                                             cgcgcgGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTG.GCCCCGCCTCGAAGGGCGGCGGCACGACGCGGCGCACCTTCGGCTCGGGCGGCTCCATc                                        <  1:8852198/101‑1 (MQ=255)
                                                                gcgTCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTG.GCCCCGCCTCGAAGGGCGGCGGCACGACGCGGCGCACCTTCGGTTCGGGCGGCGCCATCAGc                                     <  2:2856521/101‑1 (MQ=255)
                                                                gcgGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTG.GCCCCGCCTCGAAGGGCGGCGGCACGACGCGGCGCACCTTCGGTTCGGGCGGCGCCATcgcc                                     <  2:7949013/101‑4 (MQ=255)
                                                                 cgGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTT.GGCCCGCCTCGAAGGGCGGCGGCACGACGCGGCGCACCTTCGGTTCGGGCGGGGCCATCAgac                                    >  2:6482496/1‑99 (MQ=255)
                                                                 cgGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTT.GCCCCGCCTCGAAGGGCGGCGGCACGACGCGGGGGCCCCTCGGGTCGGGGGGCGCCCTCAGcc                                    >  1:7101974/1‑100 (MQ=255)
                                                                    cccGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTG.GCCCCGCCTCGAAGGGCGGCGGCACGACGCGGCGCACCTTCGGTTCGGGCGGCGCCATCAGCtctt                                 <  2:3549875/101‑1 (MQ=255)
                                                                      cGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTT.GCCCCCCCTCGAAGGGCGGCGGGACGACGCGGCGCACCTTCGGGTCGGGCGGCGCCATCAGCtcttct                               >  2:295710/1‑101 (MQ=255)
                                                                      cGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTG.GCCCCGCCTCGAAGGGCGGCGGCACGACGCGGCGCACCTTCGGTTCGGGCGGCGCCATCAGCtcttct                               <  2:5338491/101‑1 (MQ=255)
                                                                      cGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTG.GCCCCGCCTCGAAGGGCGGCGGCACGACGCGGCGCACCTTCGGTTCGGGCGGCGCCATCAGCtcttct                               <  2:8993994/101‑1 (MQ=255)
                                                                        cTGGGCGCCGATTTCCCGGCAAAGGGGC.TTT.GCGCCGCCTCGCAGTGAGGCGGCACGGCGCGGCGCACCTTCCGTTCGGGGGTCGTCCTCAGATCttcttc                             >  2:4120157/1‑101 (MQ=255)
                                                                        cTGGGCGCCGATTTCCCGGCAAAGGGGC.TTT.GCCCCGCCTCGAAGGGCGGCGGGACGACGCGGCGCACCTTCGGTTCGGGCGGGGCCCCCAGCTCttcttc                             >  2:7827898/1‑101 (MQ=255)
                                                                         tGGGCGCCGATTTCCCGGCAAAGGGGC.TTG.GCCCCGCCTCGCAGGGCGGGGGCACGACGCGGCGCACCTTCGGTTCGGGCGGCGCCCTCCGCTCTTCCTcc                            >  2:8617859/1‑101 (MQ=255)
                                                                         tGGGCGCCGATTTCCCGGCAAAGGGGC.TTG.GCCCCGCCTCGAAGGGCGGCGGCACGACGCGGCGCACCTTCGGTTCGGGCGGCGCCATCAGCTCTTCTTcc                            >  2:7244354/1‑101 (MQ=255)
                                                                         tGGGCGCCGATTTCCCGGCAAAGGGGC.TTG.GCCCCGCCTCGAAGGGCGGCGGCACGACGCGGCGCACCTTCGGTTCGGGCGGCGCCATCAGCTCTTCTTcc                            <  1:5583411/101‑1 (MQ=255)
                                                                          gggCGCCGATTTCCCGGCAAAGGGGC.TTT.GCCCCGCCCCGAAGGGCGGCGGCACGACGCGGCGCACCTTCGGTTCCGGCGGCGCCCCCAGCCCCTCTTccc                           >  2:5159304/1‑100 (MQ=255)
                                                                          gggCGCCGATTTCCCGGCAAAGGGGC.TTT.GCCCCCCCCCGCAGGGGGGGGGCACGACGCGGCGCCCCCTCGGGTCGGGCGGGGCCCTCAGCCcttctttcc                           >  1:7947900/1‑98 (MQ=255)
                                                                                cGATTTCCCGGGCAAGGGGC.TTG.CCCCCGCCTCGAAGGGCGGCGGCACGACGCGGCGCCCCTTCGGTTCGGGCGGCGCCATCAGCTCTTCTTCCTCGTGtt                     <  2:1021565/101‑1 (MQ=255)
                                                                                cGATTTCCCGGCAAAGGGGC.TTG.GCCCCGCCTCGAAGGGCGGCGGCACGACGCGGCGCACCTTCGGTTCGGGCGGCGCCATCAGCTCTTCTTCCTCGTGtt                     <  2:3995128/101‑1 (MQ=255)
                                                                                cGATTTCCCGGCAAAGGGGC.TTG.GCCCCGCCTCGAAGGGCGGCGGCACGACGCGGCGCACCTTCGGTTCGGGCGGCGCCATCAGCTCTTCTTCCTCGTGtt                     >  1:2101790/1‑101 (MQ=255)
                                                                                 gATTTCCCGGCAAAGGGGC.TTT.GCCCCCCCTCGAAGGGCGGGGGCACGACGCGGCGCACCTTCGGTTCGGGCGGCGCCCTCAGCTCTTCTTCCTCGTGTTc                    >  1:2722822/1‑101 (MQ=255)
                                                                                 gATTTCCCGGCAAAGGGGC.TTG.GCCCCGCCTCGAAGGGCGGCGGCACGACGCGGCGCACCTTCGGTTCGGGCGGCGCCATCAGCTCTTCTTCCTCGTGTTc                    <  2:5959085/101‑1 (MQ=255)
                                                                                  gtttCCCGGCAAAGGGGC.TTG.GCCCCGCCTCGAAGGGCGGCGGCACGACGCGGCGCACCTTCGGTTCGGGCGGCGCCATCAGCTCTTCTTCCTCGTGTTCg                   <  1:4713513/100‑1 (MQ=255)
                                                                                  aTTTCCCGGCAAAGGGGC.TTG.GCCCCGCCTCGAAGGGCGGCGGCACGACGCGGCGCACCTTCGGTTCGGGCGGCGCCATCAGCTCTTCTTCCTCGTGTTCg                   <  1:4537204/101‑1 (MQ=255)
                                                                                   tttCCCGGCAAAGGGGC.TTT.GCCCCGCCTCGAAGGGCGGGGGCCCGACGCGGCGCCCCTTCGGTTCGGGCGGGGCCATCAGCCCTTCTTCCTCGTGtttgc                  >  1:4905837/1‑98 (MQ=255)
                                                                                     tCCCGGCAAAGGGGC.TTG.GCCCCGCCTCGAAGGGCGGCGGCACGACGCGGCGCACCTTCGGTTCGGGCGGCGCCATCAGCTCTTCTTCCTCGTGTTCGATc                <  2:8060102/101‑1 (MQ=255)
                                                                                      cccGGCAAAGGGGC.TTG.GCCCCGCCTCGAAGGGCGGCGGCACGACGCGGCGCACCTTCGGTTCGGGCGGCGCCATCAGCTCTTCTTCCTCGTGTTCGATCa               <  1:5285935/101‑1 (MQ=255)
                                                                                      cccGGCAAAGGGGC.TTG.GCCCCGCCTCGAAGGGCGGCGGCACGACGCGGCGCACCTTCGGTTCGGGCGGCGCCATCAGCTCTTCTTCCTCGTGTTCGATCa               <  2:2714690/101‑1 (MQ=255)
                                                                                       ccGGCAAAGGGGC.TTG.GCCCCGCCTCGAAGGGCGGCGGCACGACGCGGCGCACCTTCGGTTCGGGCGGCGCCATCAGCTCTTCTTCCTCGTGTTCGATCAg              <  1:4250383/101‑1 (MQ=255)
                                                                                        cGGCAAAGGGGC.TTG.GCCCCGCCTCGAAGGGCGGCGGCACGACGCGGCGCACCTTCGGTTCGGGCGGCGCCATCAGCTCTTCTTCCTCGTGTTCGATCAGc             >  2:3709804/1‑101 (MQ=255)
                                                                                            aaaGGGGC.TTT.GCCCCCCCTCGAAGGGCGGCGGCACGACGCGGCGCCCCTTCGGTTCGGGCGGCGCCCACAGCTCTTCCTACTCGTGGTTGATCAGCCGcc         >  2:9073192/1‑100 (MQ=255)
                                                                                            aaaGGGGC.TTG.GCCCCGCCTCGAAGGGCGGCGGCACGACGCGGCGCACCTTCGGTTCGGGCGGCGCCATCAGCTCTTCTTCCTCGTGTTCGATCCGCCGcc         >  2:3136422/1‑100 (MQ=255)
                                                                                            aaaGGGGC.TTG.GCCCCGCCTCGAAGGGCGGCGGCACGACGCGGCGCACCTTCGGTTCGGGCGGCGCCATCAGCTCTTCTTCCTCGTGTTCGATCAGCCGCa         >  2:3193612/1‑101 (MQ=255)
                                                                                              aGGGGC.TTG.GCCCCGCCTCGAAGGGCGGCGGCACGACGCGGCGCACCTTCGGTTCGGGCGGCGCCATCAGCTCTTCTTCCTCGTGTTCGATCAGCCGCAcc       <  2:7015067/101‑1 (MQ=255)
                                                                                               ggggC.TTG.GCCCCGCCTCGAAGGGCGGCGGCACGACGCGGCGCACCTTTGGTTCGGGCGGCGCCATCAGCTCCTCTTCCTCGGGTTTGATCCCCCCCAccc      >  1:3531499/1‑100 (MQ=255)
                                                                                               ggggC.TTG.GCCCCGCCTCGAAGGGCGGCGGCACGACGCGGCGCACCTTCGGTTCGGGCGGCGCCATCAGCTCTTCTTCCTCGTGTTCGGTCAGCCGCACcg      <  1:6507119/101‑1 (MQ=255)
                                                                                                gggC.TTG.GCCCCGCCTCGAAGGGCGGCGGCACGACGCGGCGCACCTTCGGTTCGGGCGGCGCCATCAGCTCTTCTTCCTCGTGTTCGATCAGCCGCACcgc     >  2:8339065/1‑101 (MQ=255)
                                                                                                gggC.TTG.GCCCCGCCTCGAAGGGCGGCGGCACGACGCGGCGCACCTTCGGTTCGGGCGGCGCCATCAGCTCTTCTTCCTCGTGTTCGATCAGCCGCACcgc     <  2:1325153/101‑1 (MQ=255)
                                                                                                gggC.TTG.GCCCCGCCTCGAAGGGCGGCGGCACGACGCGGCGCACCTTCGGTTCGGGCGGCGCCATCAGCTCTTCTTCCTCGTGTTCGATCAGCCGCACcgc     >  1:1032966/1‑101 (MQ=255)
                                                                                                   c.TTG.GCCCCGCCTCGAAGGGCGGCGGCACGACGCGGCGCACCTTCGGTTCGGGCGGCGCCATCAGCTCTTCTTCCTCGTGTTCGATCAGCCGCACcgcgcg  >  2:7597542/1‑101 (MQ=255)
                                                                                                    |                                                                                                     
AGACCGCCGCCACTGCCGGGCAGCGGATCATAGGCCGGGGAATCGATCCGGTCGAGCGGCGCGCGCGGCCCGCTGGGCGCCGATTTCCCGGCAAAGGGGC.TTG.GCCCCGCCTCGAAGGGCGGCGGCACGACGCGGCGCACCTTCGGTTCGGGCGGCGCCATCAGCTCTTCTTCCTCGTGTTCGATCAGCCGCACCGCGCG  >  NC_014034/2780311‑2780510

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 28 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG