BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	279,645	+A	4.1%	intergenic (‑47/‑33)	RCAP_rcc00242 ← / → dapD	hypothetical protein/2,3,4,5‑tetrahydropyridine‑2,6‑dicarboxylate N‑succinyltransferase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	279,645	0	.→A	4.1%	74.9 ‑4.6 ‑1.3	533	intergenic (‑47/‑33)	RCAP_rcc00242/dapD	hypothetical protein/2,3,4,5‑tetrahydropyridine‑2,6‑dicarboxylate N‑succinyltransferase
Reads supporting (aligned to +/- strand): new base (16/4): ref base (250/260): total (269/264)
Fisher's exact test strand distribution p-value = 1.03e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.75e-02

Only 100 of 572 total aligned reads displayed.


TTTTGCATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAACCCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACACCCTCACGCC  >  NC_014034/279545‑279742


ttttGCATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAAc                                                                                                   >  2:6718593/1‑101 (MQ=255)


ttttGCATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAAc                                                                                                   >  2:3699830/1‑101 (MQ=255)


 tttGCATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAAcc                                                                                                  >  1:7001334/1‑101 (MQ=255)


  ttGCATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGCAAAcca                                                                                                 >  2:1973956/1‑100 (MQ=255)


  ttGCATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAAcca                                                                                                 <  2:7691706/101‑2 (MQ=255)


   tGCATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAAccat                                                                                                >  2:634214/1‑99 (MQ=255)


    gCATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAAccatg                                                                                               >  1:7460828/1‑98 (MQ=255)


     cATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGCGGGGCTTTCCCCAACTATAGGGGGAAAAccatga                                                                                              >  1:1001208/1‑97 (MQ=255)


     cATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAAccatga                                                                                              >  2:2186914/1‑97 (MQ=255)


        ccATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATg                                                                                          <  1:819761/101‑1 (MQ=255)


          atgatgCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATgac                                                                                        <  1:5479877/101‑3 (MQ=255)


          atgatgCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGcc                                                                                        >  1:2792934/1‑101 (MQ=255)


          atgatgCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGcc                                                                                        <  2:8142864/101‑1 (MQ=255)


            gatgCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGCAAA.CCATGAATGCCAc                                                                                      >  2:3543370/1‑101 (MQ=255)


            gatgCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCAc                                                                                      >  2:7780629/1‑101 (MQ=255)


            gatgCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCAc                                                                                      >  1:2233075/1‑101 (MQ=255)


             atgCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACt                                                                                     <  2:2687094/101‑1 (MQ=255)


                 tGTCACGGAATGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGaa                                                                                 <  1:5727365/101‑1 (MQ=255)


                 tGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGaa                                                                                 >  2:2847572/1‑101 (MQ=255)


                 tGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGaa                                                                                 >  1:3960384/1‑101 (MQ=255)


                     aCGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAagag                                                                             <  1:1801700/101‑1 (MQ=255)


                      cGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAgg                                                                            >  1:8861324/1‑101 (MQ=255)


                      cGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAgg                                                                            <  1:6709111/101‑1 (MQ=255)


                         aaCGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGAcc                                                                         >  2:3317418/1‑101 (MQ=255)


                         aaCGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGAcc                                                                         <  1:1886526/101‑1 (MQ=255)


                          aCGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCg                                                                        >  1:4995840/1‑101 (MQ=255)


                           ctggTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGt                                                                       <  1:712517/99‑1 (MQ=255)


                            gggTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGtt                                                                      >  1:414818/1‑101 (MQ=255)


                            gggTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGtt                                                                      >  1:2540043/1‑101 (MQ=255)


                            gggTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGtt                                                                      >  2:5536118/1‑101 (MQ=255)


                             ggTGGGACCGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAAGGCCACTTGAAAGAGGACCGTTc                                                                     >  2:8882080/1‑101 (MQ=255)


                             ggTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTc                                                                     <  2:1650699/101‑1 (MQ=255)


                             ggTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTc                                                                     <  2:4026150/101‑1 (MQ=255)


                             ggTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTc                                                                     <  2:6842074/101‑1 (MQ=255)


                              gTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTcc                                                                    <  1:6238817/101‑1 (MQ=255)


                               tGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCg                                                                   >  2:8218531/1‑101 (MQ=255)


                               tGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCg                                                                   >  1:8731768/1‑101 (MQ=255)


                               tGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCg                                                                   >  1:8493358/1‑101 (MQ=255)


                                 ggAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGAt                                                                 <  1:511552/101‑1 (MQ=255)


                                 ggAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGAt                                                                 >  2:5952198/1‑101 (MQ=255)


                                 ggAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGAt                                                                 >  2:3846328/1‑101 (MQ=255)


                                 ggAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGAt                                                                 >  1:4726178/1‑101 (MQ=255)


                                  gAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATg                                                                >  2:4912854/1‑101 (MQ=255)


                                   aaCGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGt                                                               <  2:5087162/101‑1 (MQ=255)


                                     cGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCg                                                             <  1:6372131/101‑1 (MQ=255)


                                      tgCTTTCGTCTTTCATAACCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGa                                                            <  1:7895648/100‑1 (MQ=255)


                                       gCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGaa                                                           >  1:1539888/1‑101 (MQ=255)


                                         tttCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACg                                                         >  1:5886810/1‑101 (MQ=255)


                                         tttCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACg                                                         >  2:5383907/1‑101 (MQ=255)


                                             gTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCAt                                                     >  1:359300/1‑101 (MQ=255)


                                              tCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATg                                                    <  2:8492978/101‑1 (MQ=255)


                                                tttCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCt                                                  <  1:2325742/101‑1 (MQ=255)


                                                tttCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCt                                                  >  2:1345772/1‑101 (MQ=255)


                                                 ttCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTc                                                 <  1:3679708/101‑1 (MQ=255)


                                                  tCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCg                                                <  1:3820917/101‑1 (MQ=255)


                                                  tCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCg                                                >  2:1468757/1‑101 (MQ=255)


                                                   cATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGa                                               >  1:8099689/1‑101 (MQ=255)


                                                     tatCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAg                                             >  1:6891898/1‑101 (MQ=255)


                                                     tatCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAg                                             >  2:9046995/1‑101 (MQ=255)


                                                      atCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGCACCGTTCCGATGTCGAACGCCATGCTCGAGGc                                            >  1:3422169/1‑101 (MQ=255)


                                                        ccTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTg                                          <  1:6128570/101‑1 (MQ=255)


                                                           gCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCa                                       <  1:4860382/101‑1 (MQ=255)


                                                           gCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCa                                       >  2:1828139/1‑101 (MQ=255)


                                                           gCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCa                                       <  1:1142739/101‑1 (MQ=255)


                                                            cTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCGTGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCAt                                      >  2:4366332/1‑101 (MQ=255)


                                                             tCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGACAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGACGTCGAACGCCATGCTCGAAGCTGCCATc                                     <  1:6511516/101‑1 (MQ=255)


                                                              cATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCg                                    <  2:3092147/101‑1 (MQ=255)


                                                               aTTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGa                                   >  1:7778231/1‑101 (MQ=255)


                                                               aTTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGa                                   >  1:973207/1‑101 (MQ=255)


                                                                ttGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGaa                                  <  2:7595538/101‑1 (MQ=255)


                                                                 tGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAg                                 <  1:167182/101‑1 (MQ=255)


                                                                  gcgcGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAgc                                <  1:5966348/101‑1 (MQ=255)


                                                                       aGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCt                           <  1:8566959/101‑1 (MQ=255)


                                                                        ggggCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTg                          >  1:767112/1‑101 (MQ=255)


                                                                          ggCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTggg                        <  1:1378094/101‑1 (MQ=255)


                                                                          ggCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTggg                        >  2:8689344/1‑101 (MQ=255)


                                                                            cTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAg                      >  2:6518510/1‑101 (MQ=255)


                                                                            cTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAg                      <  2:6134930/101‑1 (MQ=255)


                                                                             tttCCCCAACTATAGGGGGAAAA.CCATGAATGTCACTTGAAAGAGGACCGTTCCGATGACGAACGCCATGCTCGAAGCTGCCATCGAAGCGGCCTGGGAgg                     >  2:5773897/1‑101 (MQ=255)


                                                                               tCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGtt                   <  1:107597/101‑1 (MQ=255)


                                                                               tCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGtt                   <  2:796863/101‑1 (MQ=255)


                                                                               tCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGtt                   <  2:7159040/101‑1 (MQ=255)


                                                                                ccccAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTc                  <  2:7460504/101‑1 (MQ=255)


                                                                                  ccAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTcgc                <  2:2539970/101‑1 (MQ=255)


                                                                                  ccAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTcgc                >  2:1182841/1‑101 (MQ=255)


                                                                                   cAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTcgcg               <  2:4117856/101‑1 (MQ=255)


                                                                                   cAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTcgcg               >  1:9089970/1‑101 (MQ=255)


                                                                                    aaCTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGa              >  1:4516695/1‑101 (MQ=255)


                                                                                    aaCTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGa              >  1:3080439/1‑101 (MQ=255)


                                                                                      cTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGaca            >  1:8925673/1‑101 (MQ=255)


                                                                                        ataGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACAcc          <  2:9116576/101‑1 (MQ=255)


                                                                                        ataGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACAcc          <  2:2101686/101‑1 (MQ=255)


                                                                                        ataGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACAcc          >  1:4384762/1‑101 (MQ=255)


                                                                                           gggggAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACACCCTc       <  1:4655433/101‑1 (MQ=255)


                                                                                           gggggAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACACCCTc       <  1:4051386/101‑1 (MQ=255)


                                                                                           gggggAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACACACTc       <  1:8815098/101‑1 (MQ=255)


                                                                                            ggggAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACACCCTCa      <  1:7266330/101‑1 (MQ=255)


                                                                                               ggaaaACCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACACCCTCACg    <  2:4730170/99‑1 (MQ=255)


                                                                                                 aaaACCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACACCCTCACGcc  <  2:2251083/101‑1 (MQ=255)


                                                                                                 aaaACCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACACCCTCACGcc  >  1:758249/1‑101 (MQ=255)


TTTTGCATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAACCCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACACCCTCACGCC  >  NC_014034/279545‑279742

Base quality scores:


ATCG

< 3 ≤


ATCG

< 25 ≤


ATCG

< 31 ≤


ATCG

< 35 ≤


ATCG

< 41 ≤


ATCG