Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,880,581 +G 4.7% coding (1004/1053 nt) pdxA ← 4‑hydroxythreonine‑4‑phosphate dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,880,5819.→G 4.7% 2.9 0.0 ‑0.064coding (1004/1053 nt)pdxA4‑hydroxythreonine‑4‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  new base (3/0):  ref base (56/5):  total (59/5)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.62e-01

GCGCAAGGGCGGCAGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GC.........C..CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGCGGGC  >  NC_014034/2880481‑2880635
                                                                                                               |                                                                
gggcAAGGGCGGCAGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCggcg..........gc                                                                   <  2:5228222/99‑1 (MQ=255)
gcgcAAGGGCGGCAGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCggcg..........gc                                                                   >  2:8981381/1‑101 (MQ=255)
gcgcAAGGGCGGCAGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCggcg..........gc                                                                   >  2:219829/1‑101 (MQ=255)
  gcAAGGGCGGCAGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCggcg..........gcgc                                                                 >  1:1618120/1‑99 (MQ=255)
   cAAGGGCGGCAGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GC.........g..cg                                                     >  1:8936261/1‑101 (MQ=255)
    aaGGGCGGCAGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GC.........g..cgt                                                    >  1:476800/1‑100 (MQ=255)
    aaGGGCGGCAGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GC.........g..cgt                                                    >  2:7590196/1‑100 (MQ=255)
    aaGGGCGGCAGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GC.........g..cgc                                                    >  2:2357152/1‑101 (MQ=255)
     aGGGCGGCAGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GC.........g..cgtc                                                   <  2:5039889/101‑3 (MQ=255)
      gggCGGCAGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GC.........g..cgtca                                                  >  2:592075/1‑98 (MQ=255)
      gggCGGCAGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GC.........g..cgtca                                                  <  2:5064052/101‑4 (MQ=255)
      gggCGGCAGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GC.........g..cgtca                                                  >  1:3199919/1‑98 (MQ=255)
       ggcggcAGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GC.........g..cgtcac                                                 <  2:7002756/101‑5 (MQ=255)
       ggcggcAGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GC.........g..cgtcac                                                 <  2:7153059/101‑5 (MQ=255)
       ggcggcACCCCGCCAATCGCAGCCAAACCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GC.........g..cgtcac                                                 <  1:2479975/101‑5 (MQ=25)
        gcgccACCCCGTCAATCGCAGCCAAACCCCGCCCCCTTACTTTTCATCTTGCTTCAAATACCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GC.........g..cgtcaca                                                <  1:6282027/101‑6 (MQ=21)
          ggcAGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GC.........g..cgtcacagc                                              >  1:3992363/1‑94 (MQ=255)
           gcAGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCGTCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GC.........g..cgtcacagcc                                             >  2:4425404/1‑93 (MQ=25)
            cAGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGATTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GC.........G..CGCCAccgccc                                            >  1:3134677/1‑100 (MQ=25)
             aGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GC.........g..cgtcacagccgc                                           <  2:8592739/101‑11 (MQ=25)
             aGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GC.........g..cgtcacagccgc                                           <  2:1669002/101‑11 (MQ=25)
             aGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GC.........g..cgtcacagccgc                                           >  1:3741305/1‑91 (MQ=25)
             aGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GC.........g..cgtcacagccgc                                           >  2:4766217/1‑91 (MQ=25)
             aGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GC.........g..cgtcacagcccc                                           >  1:201559/1‑91 (MQ=25)
                  gTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GC.........G..CGCCAcacccgcgccgc                                      >  2:6837250/1‑94 (MQ=25)
                                                ttttCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCcgc                   >  1:1409073/1‑101 (MQ=2)
                                                 tttCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCcgcg                  >  1:4939610/1‑101 (MQ=2)
                                                 tttCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCcgcg                  >  1:1487054/1‑101 (MQ=2)
                                                 tttCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCcgcg                  >  1:563889/1‑101 (MQ=2)
                                                 tttCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCcgcg                  >  2:4117887/1‑101 (MQ=2)
                                                 tttCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCcgcg                  >  1:8931581/1‑101 (MQ=2)
                                                  ttCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGa                 >  2:2376844/1‑101 (MQ=9)
                                                  ttCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGa                 >  2:3042249/1‑101 (MQ=9)
                                                  ttCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGa                 >  1:5586325/1‑101 (MQ=9)
                                                  ttCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGa                 >  1:5775932/1‑101 (MQ=9)
                                                  ttCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGa                 >  1:2246696/1‑101 (MQ=9)
                                                   tCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGAt                >  2:3274307/1‑101 (MQ=9)
                                                   tCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGAt                >  1:8190823/1‑101 (MQ=9)
                                                   tCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGAt                >  2:5916653/1‑101 (MQ=9)
                                                   tCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGAt                >  1:6019347/1‑101 (MQ=9)
                                                    cATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATc               <  2:5961503/101‑1 (MQ=9)
                                                    cATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATc               <  2:3545373/101‑1 (MQ=9)
                                                    cATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATc               >  1:4791535/1‑101 (MQ=9)
                                                     aTCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCa              >  2:6408423/1‑101 (MQ=9)
                                                     aTCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCa              >  1:327522/1‑101 (MQ=9)
                                                     aTCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCa              >  2:4146453/1‑101 (MQ=9)
                                                     aTCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCa              >  2:7245863/1‑101 (MQ=9)
                                                     aTCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCa              >  1:4419056/1‑101 (MQ=9)
                                                      tCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAg             <  2:5721480/101‑1 (MQ=9)
                                                      tCTTGCTTCAAATATCCCGGGGGCCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAg             <  2:5304953/101‑1 (MQ=9)
                                                      tCTTGCTTCAAATAGCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAg             <  2:1168776/101‑1 (MQ=9)
                                                       cTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGCTCacc            >  2:249666/1‑99 (MQ=9)
                                                       cTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGa            >  2:9175942/1‑101 (MQ=9)
                                                       cTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGa            >  1:4602987/1‑101 (MQ=9)
                                                       cTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGa            >  1:6069929/1‑101 (MQ=9)
                                                        ttGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGAc           >  1:4615642/1‑101 (MQ=9)
                                                        ttGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGAc           >  2:6970561/1‑101 (MQ=9)
                                                         tGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACt          >  2:2037564/1‑101 (MQ=9)
                                                         tGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACt          >  1:2263185/1‑101 (MQ=9)
                                                         tGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCCCCATCGCCAAGGCCGCGATCAGAcg          >  2:2991054/1‑100 (MQ=9)
                                                          gCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACtg         >  1:6478593/1‑101 (MQ=12)
                                                           cTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCGCCCATCTTCGCCCCCATCGTCAAGGCCGCGATCAGACtga        >  1:2284113/1‑100 (MQ=9)
                                                           cTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAACGCCGCGATCAGACtgt        >  1:3668593/1‑101 (MQ=9)
                                                            ttCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGTCGCGATCAGACtgtg       >  1:1606863/1‑101 (MQ=9)
                                                            ttCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACtgtg       >  1:336998/1‑101 (MQ=12)
                                                            ttCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACtgtg       >  1:314736/1‑101 (MQ=12)
                                                             tCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCTAGGCCGCGATCAGACTGTGc      >  1:8817680/1‑101 (MQ=9)
                                                             tCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGc      >  2:4718659/1‑101 (MQ=12)
                                                             tCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGc      >  2:8667336/1‑101 (MQ=12)
                                                              cAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGCg     >  1:348314/1‑101 (MQ=12)
                                                              cAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGCg     >  1:3906547/1‑101 (MQ=12)
                                                              cAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGCg     >  1:6722600/1‑101 (MQ=12)
                                                               aaaTATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGCgg    >  1:3733660/1‑101 (MQ=12)
                                                               aaaTATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGCgg    >  1:2183124/1‑101 (MQ=12)
                                                               aaaTATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGCgg    >  2:3969367/1‑101 (MQ=12)
                                                               aaaTATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGCgg    >  2:6614033/1‑101 (MQ=12)
                                                               aaaTATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGCgg    >  2:7439812/1‑101 (MQ=12)
                                                               aaaTATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGCgg    >  1:7034893/1‑101 (MQ=12)
                                                                aaTATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGCggg   >  1:6014542/1‑101 (MQ=12)
                                                                aaTATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGCggg   >  1:5324737/1‑101 (MQ=12)
                                                                aaTATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGCggg   >  2:8674439/1‑101 (MQ=12)
                                                                aaTATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGCggg   >  2:2650149/1‑101 (MQ=12)
                                                                aaTATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGCggg   >  2:1704238/1‑101 (MQ=12)
                                                                aaTATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGCggg   >  1:4114640/1‑101 (MQ=12)
                                                                aaTATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGCggg   >  2:4019446/1‑101 (MQ=12)
                                                                aaTATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGCggg   >  1:2265083/1‑101 (MQ=12)
                                                                aaTATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGCggg   >  2:5086691/1‑101 (MQ=12)
                                                                aaTATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGCggg   >  1:4080757/1‑101 (MQ=12)
                                                                 atatCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GCGCGTCACAGCCGCGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGCGGGc  >  2:6508891/1‑101 (MQ=12)
                                                                 atatCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GCGCGTCACAGCCGCGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGCGGGc  >  2:5415606/1‑101 (MQ=12)
                                                                 atatCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GCGCGTCACAGCCGCGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGCGGGc  >  1:7865820/1‑101 (MQ=12)
                                                                                                               |                                                                
GCGCAAGGGCGGCAGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GC.........C..CGCCGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGCGGGC  >  NC_014034/2880481‑2880635

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 28 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG