Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,173,565 +C 96.9% intergenic (+600/‑82) tyrA → / → RCAP_rcc02983 prephenate dehydrogenase/extensin family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,173,5651.→C96.9% 4.4 ‑1.1 ‑1.1129intergenic (+600/‑82)tyrA/RCAP_rcc02983prephenate dehydrogenase/extensin family protein
Reads supporting (aligned to +/- strand):  new base (90/35):  ref base (2/2):  total (92/37)
Fisher's exact test strand distribution p-value = 3.24e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.24e-02

Only 100 of 157 total aligned reads displayed.

GCGCCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.G.CCGCCGCGCAAGCG.C.A.A.GCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTGCGGCGTGCCCGGCCTG  >  NC_014034/3173466‑3173658
                                                                                                         |                                                                                             
gcgcCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCgcgcaagcg.c.a.a                                                                                                <  2:936471/101‑1 (MQ=255)
  gcCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCgcgcaagcg.c.a.acg                                                                                              >  2:3426586/1‑99 (MQ=255)
   ccGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCgcgcaagcg.c.a.acgc                                                                                             >  2:5575480/1‑98 (MQ=255)
    cGGTCACGCCCGCCCCCGCCACCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCgcgcaagcg.c.a.acgcc                                                                                            <  1:5615842/101‑5 (MQ=255)
      gTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.A.Cgcccc                                                                                         >  2:507133/1‑101 (MQ=255)
       tCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGccccc                                                                                         <  1:6346403/101‑1 (MQ=255)
          cggccgcccccgcccCCACCCCTCCCGCCGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCccccgcc                                                                                      <  2:5829093/98‑1 (MQ=255)
          cgcccgccCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCccccgcc                                                                                      <  1:2349995/101‑1 (MQ=255)
          cccccccccccgccCCCACCCCTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCccccgcc                                                                                      <  1:4399655/99‑1 (MQ=255)
            cccgcccccgccCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCccccgcccc                                                                                    <  2:1865446/101‑1 (MQ=255)
             ccgcccccgcccCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCccccgccccg                                                                                   >  1:3594929/1‑101 (MQ=255)
               gccccggcccccACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGa                                                                                 <  2:436715/101‑1 (MQ=255)
                  cccgcccccACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAgc                                                                              >  1:1963863/1‑101 (MQ=255)
                   ccgcccccACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAgcg                                                                             >  1:8617113/1‑101 (MQ=255)
                    cgcccccACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAgcgc                                                                            >  1:5856914/1‑101 (MQ=255)
                     gcccccACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAgggcc                                                                           >  1:7353803/1‑101 (MQ=255)
                     gcccccACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAgcgcc                                                                           >  1:5124385/1‑101 (MQ=255)
                     ccccccACCACGCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAgcgcc                                                                           <  2:8616727/100‑1 (MQ=255)
                      cccccACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAgcgccc                                                                          >  1:2420636/1‑101 (MQ=255)
                       ccccACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAgcgcccg                                                                         >  2:316308/1‑101 (MQ=255)
                          cacctcGCCCTCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGTC.CCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAgcgcccgcgc                                                                      <  2:6167039/101‑1 (MQ=255)
                           cccccTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAgcgcccgcgcc                                                                     <  1:770614/97‑1 (MQ=255)
                              ccTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGa                                                                  <  1:9186480/100‑1 (MQ=255)
                              ccTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTCCG.GCCCGCCGCGCAAGCG.C.A.ACACCCCCGCCCCGGAAGCGCCCGCGCCCGa                                                                  <  2:3586162/100‑1 (MQ=255)
                                       gCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTcc                                                         >  2:914383/1‑101 (MQ=255)
                                           ggAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGcc                                                     >  1:4362843/1‑101 (MQ=255)
                                           ggAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGcc                                                     >  1:2293324/1‑101 (MQ=255)
                                           ggAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGcc                                                     >  2:8932237/1‑101 (MQ=255)
                                            gaggccgccgccGGGCGGCGTGCTGTCGCCCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGccc                                                    <  2:4251170/98‑1 (MQ=255)
                                            gAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGccc                                                    >  2:5375283/1‑101 (MQ=255)
                                              aGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGc                                                  >  1:5355830/1‑101 (MQ=255)
                                               gccgccgccGGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCa                                                 >  2:6211010/1‑101 (MQ=255)
                                                   ccgccgGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGc                                             >  2:1284375/1‑101 (MQ=255)
                                                    cgccgGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGcc                                            <  1:7289961/101‑1 (MQ=255)
                                                     gccgGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.CAA.A.GCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCg                                           >  2:6593423/1‑101 (MQ=255)
                                                     gccgGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCg                                           <  1:3426690/101‑1 (MQ=255)
                                                      ccgGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGa                                          <  1:6863118/101‑1 (MQ=255)
                                                      ccgGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGa                                          >  2:3892388/1‑101 (MQ=255)
                                                       cgGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAg                                         >  2:3546000/1‑101 (MQ=255)
                                                       cgGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAg                                         <  2:3946132/101‑1 (MQ=255)
                                                       cgGGCGGCGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCCCCCGAAGCCCCTCCGGCCCGCACGGCCGAg                                         >  1:4743421/1‑101 (MQ=255)
                                                         ggcggcGTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGcc                                       <  1:6358220/101‑1 (MQ=255)
                                                          gcggcgTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGccc                                      <  1:3892503/101‑1 (MQ=255)
                                                          gcgccgTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCACGCGCC.A.C.GCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGccc                                      <  2:8758772/101‑1 (MQ=255)
                                                           cggcgTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCg                                     >  1:4161043/1‑101 (MQ=255)
                                                            ggcgTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCgg                                    >  1:306739/1‑101 (MQ=255)
                                                            ggcgTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCgg                                    >  1:1792333/1‑101 (MQ=255)
                                                              cgTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGcc                                  >  1:3809106/1‑101 (MQ=255)
                                                              cgTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGcc                                  >  2:167356/1‑101 (MQ=255)
                                                               gTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCt                                 >  2:7438704/1‑101 (MQ=255)
                                                               gTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCt                                 >  2:1790710/1‑101 (MQ=255)
                                                               gTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCt                                 >  1:1362848/1‑101 (MQ=255)
                                                               gTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCt                                 <  1:5375467/101‑1 (MQ=255)
                                                               gTGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCt                                 >  2:1531609/1‑101 (MQ=255)
                                                                tGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTg                                >  2:10194/1‑101 (MQ=255)
                                                                tGCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTg                                >  1:3014094/1‑101 (MQ=255)
                                                                 gCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTgc                               >  2:2078124/1‑101 (MQ=255)
                                                                 gCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTgc                               >  2:2243964/1‑101 (MQ=255)
                                                                 gCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTgc                               >  1:5023162/1‑101 (MQ=255)
                                                                 gCTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGACCCTCCGGCCCGCAGGGCCGAGCCCGGCCTgc                               >  1:4676153/1‑101 (MQ=255)
                                                                  cTGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTgcg                              >  2:7552121/1‑101 (MQ=255)
                                                                   tGTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCCGCCTgcgc                             >  2:677588/1‑101 (MQ=255)
                                                                    gTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGcgcg                            >  2:8276692/1‑101 (MQ=255)
                                                                    gTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGcgcg                            >  2:7292612/1‑101 (MQ=255)
                                                                    gTCGCGCCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGcgcg                            >  1:9644/1‑101 (MQ=255)
                                                                        cgcCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCACGGCCGAGCCCGGCCTGCGCgggcc                        >  1:6970756/1‑99 (MQ=255)
                                                                         gcCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGggtcg                       <  1:3998244/101‑1 (MQ=255)
                                                                         gcCTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGggtcg                       >  1:2643999/1‑101 (MQ=255)
                                                                           cTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGggtcggt                     >  2:373886/1‑101 (MQ=255)
                                                                           cTTTTCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGggtcggc                     >  2:8363180/1‑100 (MQ=255)
                                                                            ttttCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGggtcggtc                    >  2:2583647/1‑101 (MQ=255)
                                                                             tttCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCt                   >  1:1460253/1‑101 (MQ=255)
                                                                             tttCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCt                   <  2:3325001/101‑1 (MQ=255)
                                                                              ttCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTg                  >  1:8483257/1‑101 (MQ=255)
                                                                              ttCG.GCCCGCCGCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTg                  <  2:1702865/101‑1 (MQ=255)
                                                                                tcggcccgccgCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTgc                 <  1:5184372/96‑1 (MQ=255)
                                                                                tcggcacgccgcGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGGCGGTCCgc                 >  2:9084731/7‑101 (MQ=255)
                                                                                 cggcccgccgCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTgcg                >  2:2031419/5‑101 (MQ=255)
                                                                                 cggcccgccgCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTgcg                <  2:4203776/97‑1 (MQ=255)
                                                                                 cggcccgccgCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTgcg                >  2:4055539/5‑101 (MQ=255)
                                                                                 cggcccgccgCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTgcg                >  2:3748459/5‑101 (MQ=255)
                                                                                  ggcccgccgCGCAAGCG.C.ACC.GCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTgcgg               <  2:2420563/98‑1 (MQ=255)
                                                                                  ggcccgccgCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTgcgg               >  2:4284170/4‑101 (MQ=255)
                                                                                  ggcccgccgCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTgcgg               >  1:3408928/4‑101 (MQ=255)
                                                                                   gcccgccgCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTgcggc              >  2:8661073/3‑101 (MQ=255)
                                                                                   gcccgccgCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTgcggc              >  2:629121/3‑101 (MQ=255)
                                                                                   gcccgccgCGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTgcggc              <  2:2683764/99‑1 (MQ=255)
                                                                                    cccgccgCGCAAGCC.C.ACC.GCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTgcggcg             <  1:914396/100‑1 (MQ=255)
                                                                                       gccgcGCAAGCG.C.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCACGGGTCGGTCTGCGGCGTGc          >  1:5610294/1‑101 (MQ=255)
                                                                                         cgcgCAAGCG.C.AGC.GCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTGCGGCGTGccc        <  1:9085153/101‑1 (MQ=255)
                                                                                          gcgcaagcg.c.a.acGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTGCGGCGTGCCCg       <  2:5856679/101‑1 (MQ=255)
                                                                                          gcgcaagcg.c.a.acGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTGCGGCGTGCCCg       <  1:1757617/101‑1 (MQ=255)
                                                                                            gcaagcg.c.a.acgCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTGCGGCGTGCCCGGc     >  2:4631627/1‑101 (MQ=255)
                                                                                            gcaagcg.c.a.acgCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTGCGGCGTGCCCGGc     >  1:8162319/1‑101 (MQ=255)
                                                                                             caagcg.c.a.acgCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTGCGGCGTTCCCGGcc    <  1:8735059/101‑1 (MQ=255)
                                                                                             caagcg.c.a.acgCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTGCGGCGTGCCCGGcc    >  2:1295572/1‑101 (MQ=255)
                                                                                              aagcg.c.a.acgCCCCCGCCCCGGAAGCGCCCGCGCCCGCAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGACTGCGGCGTGCCCGGCCt   >  2:2806232/1‑101 (MQ=255)
                                                                                              aagcg.c.a.acgCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTGCGGCGTGCCCGGCCt   <  2:5209976/101‑1 (MQ=255)
                                                                                              aagcg.c.a.acgCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTGCGGCGTGCCCGGCCt   >  1:8644364/1‑101 (MQ=255)
                                                                                              aagcg.c.a.acgCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTGCGGCGTGCCCGGCCt   >  1:8874264/1‑101 (MQ=255)
                                                                                                         |                                                                                             
GCGCCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCG.G.CCGCCGCGCAAGCG.C.A.A.GCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTGCGGCGTGCCCGGCCTG  >  NC_014034/3173466‑3173658

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG