Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,254,684 +A 0.8% intergenic (+9/‑174) glpX → / → recJ fructose‑1,6‑bisphosphatase/single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,254,6841.→A 0.8% 3.4 ‑2.9 ‑0.0375intergenic (+9/‑174)glpX/recJfructose‑1,6‑bisphosphatase/single‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (3/0):  ref base (141/231):  total (144/231)
Fisher's exact test strand distribution p-value = 5.59e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.96e-01

Only 100 of 434 total aligned reads displayed.

GCAACCCGAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.CCGGGGGGTATGCCGTCCCCCGCACCCCCTG.CGC.GTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTT.GGCCGAAATATCCCGGGGGGGAATTTG  >  NC_014034/3254584‑3254780
                                                                                                     |                                                                                                   
gcAACCCGAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCt                                                                                                      <  1:8363857/101‑1 (MQ=255)
gcAACCCGAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCt                                                                                                      <  2:1868661/101‑1 (MQ=255)
gcAACCCGAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCt                                                                                                      >  1:522233/1‑101 (MQ=255)
gcAACCCGAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCt                                                                                                      >  1:4645873/1‑101 (MQ=255)
 cAACCCGAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCta                                                                                                     <  2:869972/101‑2 (MQ=255)
  aaCCCGAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCtac                                                                                                    <  1:7479271/101‑3 (MQ=255)
  aaCCCGAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCtac                                                                                                    <  2:8582423/101‑3 (MQ=255)
  aaCCCGAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCtac                                                                                                    <  2:2395665/101‑3 (MQ=255)
  aaCCCGAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCtac                                                                                                    <  2:582377/101‑3 (MQ=255)
  aaCCCGAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCtac                                                                                                    <  1:4922770/101‑3 (MQ=255)
  aaCCCGAAATTCATCGAGAACGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCtac                                                                                                    <  2:2951626/101‑3 (MQ=255)
       gAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCtacccggg                                                                                               >  2:8154050/1‑94 (MQ=255)
       gAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCtaacgggg                                                                                               >  2:6125558/1‑94 (MQ=255)
       gAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACggggg                                                                                              >  1:7003767/1‑101 (MQ=255)
        aaaTTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACggggga                                                                                             <  2:1259686/101‑2 (MQ=255)
         aaTTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggac                                                                                            <  2:4132729/101‑3 (MQ=255)
         aaTTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggac                                                                                            <  1:440341/101‑3 (MQ=255)
          aTTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggact                                                                                           <  1:9134901/101‑4 (MQ=255)
           ttCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCTACCgggggggg                                                                                           >  1:1944953/1‑99 (MQ=255)
           ttCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggactg                                                                                          <  1:7397537/101‑5 (MQ=255)
             cATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACcgggggctgcc                                                                                        >  2:6384607/1‑96 (MQ=255)
                cGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggagcgccgtc                                                                                     >  2:1745954/1‑92 (MQ=255)
                 gagaCCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggccgggcgccc                                                                                    >  1:569080/1‑91 (MQ=255)
                 gagaCCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggactgccgtcc                                                                                    <  1:5931316/101‑11 (MQ=255)
                   gaCCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTcccc                                                                                 <  2:249530/101‑1 (MQ=255)
                     ccGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCg                                                                               <  1:4108879/101‑1 (MQ=255)
                     ccGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCg                                                                               <  1:57683/101‑1 (MQ=255)
                      cGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGc                                                                              <  2:8444407/101‑1 (MQ=255)
                      cGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGCGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CCGCCGTccccccc                                                                              >  2:7118924/1‑99 (MQ=255)
                       gAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCa                                                                             <  1:8169441/101‑1 (MQ=255)
                        aaaCGATCCTGATGCGCTCGAAAGCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCAc                                                                            <  1:1046346/101‑1 (MQ=255)
                        aaaCGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGGCGGGCCGCcccccccc                                                                             >  2:5513704/1‑98 (MQ=255)
                        aaaCGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCAc                                                                            <  2:8853251/101‑1 (MQ=255)
                        aaaCGATCCTGATGCGCTCGAAAACCGGCTCGGGGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AAGGGGGGCTGGCGGCCCCCCGcc                                                                             >  2:2220625/1‑100 (MQ=255)
                           cGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCAcccc                                                                         <  2:3483893/101‑1 (MQ=255)
                              tCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTg                                                                      <  2:5679417/101‑1 (MQ=255)
                               ccTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGGCGGCCCCCGCCcccccccc                                                                     >  2:4822153/1‑98 (MQ=255)
                                 tGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.GCGCCGGCCCCCCCCcccccccccc                                                                   >  1:7804460/1‑96 (MQ=255)
                                  tccgatcTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTG.cgc.g                                                                <  2:1993633/95‑1 (MQ=255)
                                  gATGCGCTCGAACACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGGGTGGCCGCCCCCCCCCcccccccccg                                                                   >  1:6639686/1‑96 (MQ=255)
                                    tGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTG.CGC.GTa                                                              <  2:3984844/101‑1 (MQ=255)
                                     gcgcTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTG.CGC.GTAt                                                             <  1:5138522/101‑1 (MQ=255)
                                     gcgcTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTG.CGC.GTAt                                                             <  1:3998704/101‑1 (MQ=255)
                                      cgcTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTG.CGC.GTAtt                                                            <  2:5372799/101‑1 (MQ=255)
                                       gcTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTG.CGC.GTAttt                                                           <  2:140079/101‑1 (MQ=255)
                                        cTCGAACACCGGCGCGGTCCCCCGGGTGATCTATCGTAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTG.CGC.GTAtttt                                                          <  2:8959193/101‑1 (MQ=255)
                                          cGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTG.CGC.GTATTTTGc                                                        <  2:2550447/101‑1 (MQ=255)
                                          cGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCCC.CGC.GGATTTCGc                                                        >  2:9127204/1‑101 (MQ=255)
                                           gAAACCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTG.CGC.GTATTTTGcc                                                       <  1:6475692/101‑1 (MQ=255)
                                           gAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGCCGTCCCCCCCCCCCCCCG.CGC.GGATTTTgtc                                                       >  1:3088070/1‑99 (MQ=255)
                                            aaaaCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGCCCGCCCCCGCACCCCCCG.CGC.CGATTTTTCCa                                                      >  2:1569379/1‑101 (MQ=255)
                                             aaaCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTG.CGC.GTATTTTGCCaa                                                     <  1:2480649/101‑1 (MQ=255)
                                                 cGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACCGGGGG.GTGCCGGCCCCCCCCCCCCCCGCCGC.GGTTTTTGCCAAGGa                                                  >  1:940813/1‑101 (MQ=255)
                                                 cGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTG.CGC.GTATTTTGCCAAGGaa                                                 <  1:2873367/101‑1 (MQ=38)
                                                  ggCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTG.CGC.GTATTTTGCCAAGGaaa                                                <  2:7003482/101‑1 (MQ=38)
                                                  ggCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTG.CGC.GTATTTTGCCAAGGaaa                                                <  1:3765512/101‑1 (MQ=38)
                                                  ggCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTG.CGC.GTATTTTGCCAAGGaaa                                                <  1:4288383/101‑1 (MQ=38)
                                                  ggCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGATGAGGCAACGCT.AC.GGGGGAGTGCCGTCCCCCGCACCCCCCG.CGC.GTATTTTGCCaaaggaa                                                >  2:6788758/1‑96 (MQ=37)
                                                   gCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGCCGCCCCCCCCCCCCCCCG.CGC.GTATTTTTCCCAGGgaaa                                               >  1:2128608/1‑101 (MQ=255)
                                                    cTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTG.CGC.GTATTTTGCCAAGGAAAAc                                              <  2:7813418/101‑1 (MQ=38)
                                                    cTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTG.CGC.GTATTTTGCCAAGGAAAAc                                              <  2:6931098/101‑1 (MQ=38)
                                                     tCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTG.CGC.GTATTTTGCCAAGGAAAACg                                             <  1:3357132/101‑1 (MQ=38)
                                                      cGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGCCGTCCCCCGCACCCCCTC.CGC.GGATTTTGCCAAGGAAAACgg                                            >  2:4331014/1‑101 (MQ=255)
                                                       ggTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTG.CGC.GTATTTTGCCAAGGAAAACggg                                           >  1:7107277/1‑101 (MQ=38)
                                                       ggTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGGCCCCCGCACCCCCTG.CGC.GTATTTTGCCAAGGAAAACggg                                           >  2:5243311/1‑101 (MQ=38)
                                                          gcgcCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTG.CGC.GTATTTTGCCAAGGAAAACGGGGcc                                        <  1:7552210/101‑1 (MQ=38)
                                                           cgcCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTG.CGC.GTATTTTGCCAAGGAAAACGGGGCCg                                       <  2:5742219/101‑1 (MQ=38)
                                                            gcCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGCCGTCCCCCCCCCCCCCCG.CCC.CGCTTTTGGCAAGGAAAAAGAGGCCgg                                      >  1:2666997/1‑100 (MQ=255)
                                                            gcCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCCCCCCCTG.CGC.GTATTTTGCCAAGGAAAACGGGGCCGt                                      <  1:1616143/101‑1 (MQ=38)
                                                              cGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTG.CGC.GTATTTTGCCAAGGAAAACGGGGCCGttt                                    <  1:1424580/101‑1 (MQ=38)
                                                               gggTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGCCGTCCCCCGCACCCCCTG.CGC.GTATTTTGCCAAGGAAAACGGGGCCGGTTc                                   >  1:6924320/1‑101 (MQ=38)
                                                               gggTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTG.CGC.GTATTTTGCCAAGGAAAACGGGGCCGTTTc                                   <  1:1218859/101‑1 (MQ=38)
                                                                ggTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGCCGGCCCCCGCCCCCCCCG.CGC.GGATTTTTCCCAAGAAAACCGGGCCGGTTCa                                  >  1:9034837/1‑101 (MQ=255)
                                                                ggTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTG.CGC.GTATTTTGCCAAGGAAAACGGGGCCGTTTCa                                  <  1:2559284/101‑1 (MQ=38)
                                                                ggTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCCG.CGC.GTATTTTGCCAAGGAAAACGGGGCCGTTTCa                                  >  2:4615259/1‑101 (MQ=37)
                                                                  tGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGCCGTCCCCCCCACCCCCCG.CGC.GCATTTTTCCAAGGCAAAAGGGGGGGTTTCAcc                                >  2:378722/1‑101 (MQ=255)
                                                                  tGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTG.CGC.GTATTTTGCCCAGCAAAACGGGGGCGCTTCAcc                                >  2:2068132/1‑101 (MQ=255)
                                                                   gATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGCCGCCCCCCCCCCCCCCCC.CGC.CCGTTTTTCCCAGGAAAAAGGGGGCGCTTTACCt                               >  2:3226662/1‑101 (MQ=255)
                                                                   gATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGCCCCCCGCCCCCCCCG.CGC.GGATTTTGCCAAGGAAAACGGGGCCGTTTCACCt                               >  2:7966396/1‑101 (MQ=25)
                                                                    aTCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTG.CGC.GTATTTTGCCAAGGAAAACGGGGCCGTTTCACCtt                              <  2:8207457/101‑1 (MQ=38)
                                                                    aTCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTG.CGC.GTATTTTGCCAAGGAAAACGGGGCCGTTTCACCtt                              <  1:8856694/101‑1 (MQ=38)
                                                                    aTCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTG.CGC.GTATTTTGCCAAGGAAAACGGGGCCGTTTCACCtt                              <  1:5496138/101‑1 (MQ=38)
                                                                     tCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGCCGGCCCCCCCACCCCCCG.CGC.GTATTTTTTCAAGGAAAAACGGGGCGGTTCACCCT.g                            >  2:6720723/1‑101 (MQ=255)
                                                                      cTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCCG.CGC.GGATTTTGCCAAGGAAAACGGGGCCGTTTCACCTT.gg                           >  2:5981303/1‑101 (MQ=37)
                                                                       tATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGCCGTCCCCCCCACCCCCCG.CGC.GTCTTTTTCCAAAGAAAAACGGGGCGGTTCACCTT.GGc                          >  2:5069545/1‑101 (MQ=255)
                                                                        aTCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGCCCGCCCCCCCCCCCCCCG.CCC.GGATTTTTCCCAAGAAAACGGGGCCGGTTCACCtttggc                          >  1:6105985/1‑97 (MQ=255)
                                                                         tCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTG.CGC.GTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTT.GGCCg                        <  2:7516620/101‑1 (MQ=38)
                                                                         acGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTG.CGC.GTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTT.GGCCg                        <  2:2897548/100‑1 (MQ=38)
                                                                          cGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTG.CGC.GTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTT.GGCCGa                       <  2:4490259/101‑1 (MQ=38)
                                                                             aaCCCGATCCGCTGAGGCAACGCT.ACGGGGGG.GGGGCGCCCCCCCCCCCCCCCC.CGCGGCATTTTCCCAAGAAAAAAGGGGCGGTTTCCCCTTGGGCCGaa                      >  1:6277586/1‑101 (MQ=255)
                                                                             aaCCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTG.CGC.GTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTT.GGCCGAAat                    <  1:2313429/101‑1 (MQ=38)
                                                                             aaCCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCCG.CGC.GGATTTTGCCCAGGAAAACGGGGGCGGTTCACCTT.TGCCCaaaa                    >  2:2730244/1‑100 (MQ=21)
                                                                              aCCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTG.CGC.GTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTT.GGCCGAAata                   <  2:7413707/101‑1 (MQ=38)
                                                                               cccGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTG.CGC.GTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTT.GGCCGAAatat                  <  1:3430720/101‑1 (MQ=38)
                                                                                 cGATCCGCTGAGGCAACGCT.ACGGGGGG.CGGCCGGCCCCCCCCCCCCCCC.CGC.GCGTTTTTCCAAGGAAAAAGGGGGCGGTTCACCCT.GGGCGGAatattc                >  1:3488770/1‑99 (MQ=255)
                                                                                  gATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTG.CGC.GTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTT.GGCCGAAATATccc               <  1:3270273/101‑1 (MQ=38)
                                                                                  gATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTG.CGC.GTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTT.GGCCGAAATATccc               <  1:4534088/101‑1 (MQ=38)
                                                                                      cGCTGAGGCAACGCT.ACGGGGGG.CTGCCGTCCCCCGCACCCCCCG.CGC.GGATTTTTCCAAGGAAAACGGGGCCGGTTCCCCTT.GGCCGCAAACTCCCgggg           >  2:4926169/1‑101 (MQ=21)
                                                                                         tGAGGCAACGCT.ACGGGGGG.CTGCCGTCCCCCGCCCCCCCCG.CGC.GTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTT.TGCCGAAATATCCCcgggggg        >  2:3421647/1‑101 (MQ=37)
                                                                                             gCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTG.CGC.GTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTT.GGCCGAAATATCCCGGGGGGGAAtt    <  1:4822302/101‑1 (MQ=38)
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GCAACCCGAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.CCGGGGGGTATGCCGTCCCCCGCACCCCCTG.CGC.GTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTT.GGCCGAAATATCCCGGGGGGGAATTTG  >  NC_014034/3254584‑3254780

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG