Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,256,171 +C 100% coding (1314/1734 nt) recJ → single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,256,1711.→C100.0% 994.6 268coding (1314/1734 nt)recJsingle‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (148/120):  ref base (0/0):  total (148/120)

Only 100 of 321 total aligned reads displayed.

GGGGGTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGAA.CCGCGATGG.AA.GGCT.GCCGA.G.T.GTTCGCCCGGCAGGGCGC.GGCA.C.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCT  >  NC_014034/3256072‑3256257
                                                                                                     |                                                                                                 
gggggTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAgg                                                                                                    <  1:3806063/101‑1 (MQ=255)
gggggTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAgg                                                                                                    >  1:737047/1‑101 (MQ=255)
 ggggTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggc                                                                                                   <  1:4634075/101‑2 (MQ=255)
  gggTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcg                                                                                                  <  2:7367602/101‑3 (MQ=255)
   ggTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcgc                                                                                                 >  2:3625177/1‑98 (MQ=255)
   ggTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcgc                                                                                                 <  2:8721034/101‑4 (MQ=255)
   ggTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcgc                                                                                                 <  1:7615703/101‑4 (MQ=255)
     tCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcgcag                                                                                               >  2:8632569/1‑96 (MQ=255)
     tCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcgcag                                                                                               <  2:3469207/101‑6 (MQ=255)
      cGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcgcagc                                                                                              >  2:546271/1‑95 (MQ=255)
      cGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcgcagc                                                                                              >  1:2459021/1‑95 (MQ=255)
       gATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGTCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcgcagct                                                                                             >  1:5540625/1‑94 (MQ=255)
       gATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcgcagct                                                                                             <  2:1267979/101‑8 (MQ=255)
       gATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcgcagct                                                                                             <  1:1452095/101‑8 (MQ=255)
       gATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcgcagct                                                                                             >  1:689984/1‑94 (MQ=255)
       gATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcgcagct                                                                                             >  2:5953256/1‑94 (MQ=255)
       gATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcgcagct                                                                                             >  2:1316967/1‑94 (MQ=255)
       gATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcgcagct                                                                                             >  2:8899497/1‑94 (MQ=255)
       gATCGGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcgcagct                                                                                             <  2:8209499/101‑8 (MQ=255)
        aTCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcgcagctg                                                                                            <  1:7937748/101‑9 (MQ=255)
        aTCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcgcagctg                                                                                            >  1:5870875/1‑93 (MQ=255)
         tCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcgcagctgg                                                                                           <  1:4220731/101‑10 (MQ=255)
         tCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcgcagctgg                                                                                           <  2:287699/101‑10 (MQ=255)
         tCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcgcagctgg                                                                                           <  2:6295994/101‑10 (MQ=255)
          cTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcgcagctgga                                                                                          >  2:5759159/1‑91 (MQ=255)
            gggCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.c                                                                                       <  2:2249398/101‑1 (MQ=255)
               cgcgGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccg                                                                                    <  2:1881199/101‑3 (MQ=255)
                gcggcgGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccgc                                                                                   >  1:1031981/1‑98 (MQ=255)
                gcggcgGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccgc                                                                                   <  2:808361/101‑4 (MQ=255)
                 cggcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccgcg                                                                                  <  2:607689/101‑5 (MQ=255)
                  ggcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccgcga                                                                                 >  2:7486882/1‑96 (MQ=255)
                    cggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATg                                                                                >  1:4458491/1‑101 (MQ=255)
                     ggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATgg                                                                               <  2:4978538/101‑1 (MQ=255)
                       tGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggca                                                                             >  1:6220742/1‑99 (MQ=255)
                          aGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacgg                                                                          <  2:7207084/101‑6 (MQ=255)
                            cgcgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacggct                                                                        <  1:8130076/101‑8 (MQ=255)
                            cgcgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacggct                                                                        >  1:5092009/1‑94 (MQ=255)
                              cgTCGGGGCCGAGGGGCTTTTGCTCCAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGCCCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacggctgg                                                                      <  1:2864525/101‑10 (MQ=255)
                              cgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacggctgg                                                                      >  2:2541413/1‑92 (MQ=255)
                               gTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacggctggc                                                                     >  2:7610560/1‑91 (MQ=255)
                               gTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacggctggc                                                                     >  2:5356117/1‑91 (MQ=255)
                                     gCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.tt                                                            >  2:3178247/1‑100 (MQ=255)
                                      ccGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.ttg                                                           >  2:6119080/1‑99 (MQ=255)
                                        gAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.ttgct                                                         >  1:1339006/1‑97 (MQ=255)
                                         aGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.ttgctc                                                        >  1:4693013/1‑96 (MQ=255)
                                          ggggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.ttgctcg                                                       >  1:2625147/1‑95 (MQ=255)
                                           gggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.ttgctcgc                                                      >  1:7057558/1‑94 (MQ=255)
                                            ggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.ttgctcgcc                                                     >  2:2038021/1‑93 (MQ=255)
                                             gCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGccc                                                    <  2:4280859/101‑1 (MQ=255)
                                              cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCTCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCg                                                   <  1:3265597/101‑1 (MQ=255)
                                              cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGA.GTT.GCTCGCCCg                                                   <  2:5552424/101‑1 (MQ=255)
                                               ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGccccg                                                  >  2:8314791/1‑99 (MQ=255)
                                               ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGccccg                                                  >  1:4126682/1‑99 (MQ=255)
                                               ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCgg                                                  >  1:9130759/1‑101 (MQ=255)
                                                 ttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCa                                                >  2:681229/1‑101 (MQ=255)
                                                   gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGA.GTT.GCTCGCCCGGCAgg                                              <  1:1007962/101‑1 (MQ=255)
                                                     tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGA.GTT.GCTCGCCCGGCAGGgc                                            <  1:7264587/101‑1 (MQ=255)
                                                       aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGG.T.GCTCGCCCGGCAGGgcgc                                          >  2:6350983/1‑101 (MQ=255)
                                                       aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGgggc                                          >  1:2329291/1‑101 (MQ=255)
                                                       aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGgcgc                                          <  2:1121414/101‑1 (MQ=255)
                                                         gggCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGGGC.gg                                       >  1:2013306/1‑101 (MQ=255)
                                                          ggcggcGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGC.GTT.GCTCGCCCCGCAGGGCGC.ggg                                      >  2:5894973/1‑100 (MQ=255)
                                                          ggcggcGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCCGCAGGGGGC.ggg                                      >  1:367376/1‑100 (MQ=255)
                                                             ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGC.gggcgc                                   >  2:1503573/1‑97 (MQ=255)
                                                             ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGC.gggcac                                   <  2:3782344/101‑5 (MQ=255)
                                                             ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGC.gggcac                                   <  2:3239952/101‑5 (MQ=255)
                                                             ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGC.gggcac                                   >  1:8923875/1‑97 (MQ=255)
                                                             ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGC.gggcac                                   <  1:6879810/101‑5 (MQ=255)
                                                              gcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGC.gggcccg                                  >  1:4761127/1‑96 (MQ=255)
                                                               cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGGGCA.c.gg                                <  1:4060/101‑1 (MQ=255)
                                                               cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGGGCA.c.gg                                <  1:8632955/101‑1 (MQ=255)
                                                                gggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGGGCGGGCA.c.ggg                               >  1:5955741/1‑100 (MQ=255)
                                                                gggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGGGCA.c.ggg                               >  1:774849/1‑100 (MQ=255)
                                                                gggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGGGCA.c.ggg                               <  1:6493890/101‑2 (MQ=255)
                                                                 ggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGGGCA.c.gggc                              <  1:9194029/101‑3 (MQ=255)
                                                                  gCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGGGCA.c.gggcg                             <  2:650420/101‑4 (MQ=255)
                                                                   cACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGGGC.ggggacgggggg                             >  2:7691154/1‑91 (MQ=255)
                                                                      cGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.G.TAGCTCGCCCGGCAGGGCGCGGGCA.CGGGcggccc                          <  1:8203206/101‑1 (MQ=255)
                                                                       gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGGGCA.CGGGcggcccg                         <  2:5283803/101‑1 (MQ=255)
                                                                         aTGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGGGCACC.GGcgggccggc                       >  1:7133371/1‑101 (MQ=255)
                                                                         aTGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGGGCA.CGGGcggcccggc                       >  2:8817277/1‑101 (MQ=255)
                                                                          tGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGGGCA.CGGGcggcccggcc                      <  1:3625285/101‑1 (MQ=255)
                                                                          cgatctcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGGGCA.CGGGcggcccggcc                      <  2:6917509/95‑1 (MQ=255)
                                                                           ggCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGGGCGGGCC.CGGGCGGCCCGGCCg                     >  1:2196510/1‑101 (MQ=255)
                                                                             ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGAt                   <  1:4662544/101‑1 (MQ=255)
                                                                               gcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCt                 <  2:4121371/101‑1 (MQ=255)
                                                                               gcgGGTCGGACCCTGGCCGAGGCGAAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCt                 <  1:5469316/101‑1 (MQ=255)
                                                                                cgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTg                <  2:9130337/101‑1 (MQ=255)
                                                                                 gggTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGGGCA.CGGGGGGCCCGGCCGATCTGc               >  1:111214/1‑101 (MQ=255)
                                                                                  ggTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCg              >  1:1670946/1‑101 (MQ=255)
                                                                                    tCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGGGGA.CGGGCGGCCCGGCCGATCTGCggg            >  2:3374447/1‑100 (MQ=255)
                                                                                    tCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGGTCTGCGGc            >  1:8515453/1‑101 (MQ=255)
                                                                                    gcTGCCCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGc            <  1:3154997/100‑1 (MQ=255)
                                                                                     cTGCCCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCt           <  1:1284929/101‑1 (MQ=255)
                                                                                     cTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGGCCGGCCGATCTGCGGCt           >  2:9177144/1‑101 (MQ=255)
                                                                                     cTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCt           <  1:4308287/101‑1 (MQ=255)
                                                                                       gACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGGTCTGCGGCTgg         >  1:5705845/1‑101 (MQ=255)
                                                                                        aCCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGGGCACC.GGCGGGCCCGCCGATCTGCGGCTggg        >  2:4634947/1‑100 (MQ=255)
                                                                                             ggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCggg  >  1:3404501/2‑100 (MQ=255)
                                                                                             ggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTT.GCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGc   >  1:8374941/2‑101 (MQ=255)
                                                                                                     |                                                                                                 
GGGGGTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGAA.CCGCGATGG.AA.GGCT.GCCGA.G.T.GTTCGCCCGGCAGGGCGC.GGCA.C.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCT  >  NC_014034/3256072‑3256257

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG