Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,256,180 +C 100% coding (1323/1734 nt) recJ → single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,256,1801.→C100.0% 1097.8 296coding (1323/1734 nt)recJsingle‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (160/136):  ref base (0/0):  total (160/136)

Only 100 of 324 total aligned reads displayed.

GGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGAA.CCGCGATGG.AA.GGCT.GCCGA.G.TGTTCGCCCGGCAGGGCGC.GGCA.C.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGC  >  NC_014034/3256084‑3256270
                                                                                                    |                                                                                                  
gggCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.c                                                                                                   <  2:4095216/101‑1 (MQ=255)
  gcgcGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.ccc                                                                                                 <  2:5402816/101‑2 (MQ=255)
   cgcgGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccg                                                                                                <  2:1881199/101‑3 (MQ=255)
    gcggcgGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccgc                                                                                               >  1:1031981/1‑98 (MQ=255)
     cggcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccgcg                                                                                              <  2:607689/101‑5 (MQ=255)
      ggcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccgcga                                                                                             >  2:2694573/1‑96 (MQ=255)
        cggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATg                                                                                            >  1:4458491/1‑101 (MQ=255)
        cggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATg                                                                                            <  2:6639194/101‑1 (MQ=255)
        cggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATg                                                                                            >  2:2136929/1‑101 (MQ=255)
         ggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATgg                                                                                           >  1:4121490/1‑101 (MQ=255)
         ggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccgcgctgg                                                                                          <  1:7233348/101‑9 (MQ=255)
           tGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggca                                                                                         >  1:2257151/1‑99 (MQ=255)
             cAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacg                                                                                       <  1:5501994/101‑5 (MQ=255)
              aGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacgg                                                                                      <  2:7207084/101‑6 (MQ=255)
                cgcgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacggct                                                                                    >  1:5092009/1‑94 (MQ=255)
                  cgTCGGGGCCGAGGGGCTTTTGCTCCAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGCCCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacggctgg                                                                                  <  1:2864525/101‑10 (MQ=255)
                  cgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacggctgg                                                                                  >  1:4662223/1‑92 (MQ=255)
                   gTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacggctggc                                                                                 >  1:8572511/1‑91 (MQ=255)
                   gTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacggctggc                                                                                 >  2:6400326/1‑91 (MQ=255)
                   gTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacggctggc                                                                                 >  1:450819/1‑91 (MQ=255)
                          ccGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.ttg                                                                       >  2:6119080/1‑99 (MQ=255)
                           cGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.ttgc                                                                      >  2:8077826/1‑98 (MQ=255)
                           cGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.ttgc                                                                      >  1:1568134/1‑98 (MQ=255)
                            gAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.ttgct                                                                     >  1:1339006/1‑97 (MQ=255)
                             aGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAN.GGCTGGCCGA.g.ttgctc                                                                    >  2:320291/1‑96 (MQ=255)
                              ggggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.ttgctcg                                                                   >  1:2625147/1‑95 (MQ=255)
                                ggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.ttgctcacc                                                                 >  1:5821196/1‑93 (MQ=255)
                                 gCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGccc                                                                 >  1:3770760/1‑101 (MQ=255)
                                 gCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGccc                                                                 >  2:7054800/1‑101 (MQ=255)
                                  cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCTCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCg                                                                <  1:3265597/101‑1 (MQ=255)
                                   ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGA.GTTGCTCGCCCgg                                                               <  2:3651485/101‑1 (MQ=255)
                                    tttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGG.TGCTCGCCCGGc                                                              >  2:8810467/1‑101 (MQ=255)
                                    tttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGc                                                              <  2:5035689/101‑1 (MQ=255)
                                     ttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCa                                                             >  2:681229/1‑101 (MQ=255)
                                      tGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAg                                                            <  2:4619532/101‑1 (MQ=255)
                                       gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGA.GTTGCTCGCCCGGCAgg                                                           <  1:1007962/101‑1 (MQ=255)
                                        cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGGCCGGCAggg                                                          >  1:8174614/1‑101 (MQ=255)
                                        cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGA.GTTGCTCGCCCGGCAggg                                                          <  1:882202/101‑1 (MQ=255)
                                          cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGG.TGCTCGCCCCGCAGGgcg                                                        >  1:3391995/1‑101 (MQ=255)
                                          cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAggggg                                                        >  2:5346375/1‑99 (MQ=255)
                                          cAAAGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGgcg                                                        >  2:3364436/1‑101 (MQ=255)
                                           aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGgcgc                                                       <  1:2029299/101‑1 (MQ=255)
                                           aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGgcgc                                                       <  2:1121414/101‑1 (MQ=255)
                                             gggCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGC.gg                                                    >  1:6189194/1‑101 (MQ=255)
                                              ggcggcGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGGAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGC.ggg                                                   >  2:6253937/1‑100 (MQ=255)
                                              ggcggcGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCCGGGCGC.ggg                                                   >  1:7032125/1‑100 (MQ=255)
                                              ggcggcGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCCGCAGGGGGC.ggg                                                   >  1:367376/1‑100 (MQ=255)
                                                 ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGGGC.ggggag                                                >  2:5545685/1‑97 (MQ=255)
                                                 ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGC.ggggac                                                >  1:9174802/1‑97 (MQ=255)
                                                 ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGC.gggcgc                                                >  2:1503573/1‑97 (MQ=255)
                                                 ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGC.gggcac                                                <  1:5953491/101‑5 (MQ=255)
                                                 ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGC.gggcac                                                <  1:6879810/101‑5 (MQ=255)
                                                  gcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGC.gggcccg                                               >  1:4761127/1‑96 (MQ=255)
                                                   cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGCGGGCA.c.gg                                             <  1:4060/101‑1 (MQ=255)
                                                   cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGCGGGCA.c.gg                                             <  2:8187493/101‑1 (MQ=255)
                                                    gggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGCGGGCA.c.ggg                                            <  1:6493890/101‑2 (MQ=255)
                                                    gggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGCGGGCA.c.ggg                                            >  1:774849/1‑100 (MQ=255)
                                                     ggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGCTGGCAC.GACTGGCCGA.GTTGCTCGCCCGGCAGGGCGC.ggggcagggg                                            >  2:1272858/1‑93 (MQ=255)
                                                     ggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCCGGGCGCGGGCA.c.gggg                                           >  1:4185720/1‑99 (MQ=255)
                                                      gCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGGGC.ggggccgggcg                                           >  2:2375416/1‑92 (MQ=255)
                                                       cACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGCGGGCA.c.gggcgg                                         <  1:467437/101‑5 (MQ=255)
                                                        aCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGCGGGCA.c.gggcggc                                        >  1:7434105/1‑96 (MQ=255)
                                                         ccGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGcggcc                                        <  1:1770970/101‑1 (MQ=255)
                                                           gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGcggcccg                                      <  2:737034/101‑1 (MQ=255)
                                                             aTGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGCGGGCACC.GGcgggccggc                                    >  1:7133371/1‑101 (MQ=255)
                                                                 ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGAt                                <  2:3711334/101‑1 (MQ=255)
                                                                  cgcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATc                               <  2:8831580/101‑1 (MQ=255)
                                                                    cgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTg                             <  2:9130337/101‑1 (MQ=255)
                                                                    cgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTg                             <  2:7682850/101‑1 (MQ=255)
                                                                     gggTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGGGGCCCGGCCGATCTGc                            >  1:111214/1‑101 (MQ=255)
                                                                     gggTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGGTCTgg                            >  2:8272902/1‑100 (MQ=255)
                                                                      ggTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCg                           >  1:1670946/1‑101 (MQ=255)
                                                                       gTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCgg                          >  2:8592640/1‑101 (MQ=255)
                                                                         cTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGGCCGGCCGATCTGCGGCt                        >  2:9177144/1‑101 (MQ=255)
                                                                         cTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCt                        <  1:4308287/101‑1 (MQ=255)
                                                                         cTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCt                        <  1:7622672/101‑1 (MQ=255)
                                                                          tGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCCATCTGCGGcgg                       >  1:8972827/1‑99 (MQ=255)
                                                                           gACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCGgg                      >  1:5341767/1‑101 (MQ=255)
                                                                             ccctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGAc                    <  2:3391891/96‑1 (MQ=255)
                                                                               ctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACgg                  <  1:2375483/98‑1 (MQ=255)
                                                                                tggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCggg                 >  2:7523995/3‑101 (MQ=255)
                                                                                tggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACggg                 <  1:1211983/99‑1 (MQ=255)
                                                                                 ggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGc                >  1:8374941/2‑101 (MQ=255)
                                                                                 ggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCCATCTGCGGCTGGGCGGGc                >  2:5892003/2‑101 (MQ=255)
                                                                                  gCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGcc               >  2:2016306/1‑100 (MQ=255)
                                                                                      aggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTg           <  2:5970267/92‑1 (MQ=255)
                                                                                       ggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGa          <  1:7531224/93‑1 (MQ=255)
                                                                                       ggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGa          <  2:3561395/93‑1 (MQ=255)
                                                                                       ggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGa          <  2:4692864/93‑1 (MQ=255)
                                                                                        gcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGAt         <  1:6312857/94‑1 (MQ=255)
                                                                                        gcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGAt         <  2:4251960/94‑1 (MQ=255)
                                                                                         cgcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCCGGACGGGCGCCCGATg        >  1:7073006/7‑101 (MQ=255)
                                                                                          gcagctGGAACCCGCGATGGTAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGGCGATCTGCGGCTGGGCGGGGTCCCGGTGc       >  1:8223873/6‑101 (MQ=255)
                                                                                          gcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGc       <  1:3486766/96‑1 (MQ=255)
                                                                                            agctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGGGCGGGGA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCCGATGCCg     >  1:316393/4‑101 (MQ=255)
                                                                                            agctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGGTCCCGATGCCg     >  1:98479/4‑101 (MQ=255)
                                                                                            agctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCg     <  1:5701232/98‑1 (MQ=255)
                                                                                             gctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCCGATGCCgg    >  1:8496285/3‑101 (MQ=255)
                                                                                              ctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCCGATGCCggg   >  1:804793/2‑101 (MQ=255)
                                                                                               tGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCgggg  >  2:5704586/1‑100 (MQ=255)
                                                                                                    |                                                                                                  
GGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGAA.CCGCGATGG.AA.GGCT.GCCGA.G.TGTTCGCCCGGCAGGGCGC.GGCA.C.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGC  >  NC_014034/3256084‑3256270

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG