Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,256,200 +G 1.4% coding (1343/1734 nt) recJ → single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,256,2001.→G 1.4% 2.0 ‑2.1 0.0277coding (1343/1734 nt)recJsingle‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (4/0):  ref base (138/135):  total (142/135)
Fisher's exact test strand distribution p-value = 1.23e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 313 total aligned reads displayed.

GGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGAA.CCGCGATGG.AA.GGCT.GCCGA.G.TGTTCGCCCGGCAGGGC.GC..GGCAC.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGA  >  NC_014034/3256108‑3256293
                                                                                                    |                                                                                                  
ggCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.t                                                                                                 >  1:7183597/1‑101 (MQ=255)
 gCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.tt                                                                                                >  2:3178247/1‑100 (MQ=255)
 gCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.tt                                                                                                >  2:4116833/1‑100 (MQ=255)
 gCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.tt                                                                                                <  1:5194905/101‑2 (MQ=255)
  ccGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.ttg                                                                                               <  1:1368020/101‑3 (MQ=255)
  ccGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.ttg                                                                                               >  2:6119080/1‑99 (MQ=255)
  ccGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGA.g.ttg                                                                                               <  2:5384092/101‑3 (MQ=255)
    gAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.ttgct                                                                                             <  1:4373502/101‑5 (MQ=255)
     aGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.ttgctc                                                                                            >  1:1287830/1‑96 (MQ=255)
      ggggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGA.g.ttgctcg                                                                                           <  2:37534/101‑7 (MQ=255)
       gggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.ttgctcgc                                                                                          >  1:7103778/1‑94 (MQ=255)
        ggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.g.ttgctcacc                                                                                         >  1:5821196/1‑93 (MQ=255)
         gCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGccc                                                                                         >  1:3770760/1‑101 (MQ=255)
          cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCg                                                                                        >  1:4252076/1‑101 (MQ=255)
           ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCgg                                                                                       >  1:9130759/1‑101 (MQ=255)
            tttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGG.TGCTCGCCCGGc                                                                                      >  2:8810467/1‑101 (MQ=255)
             ttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGcc                                                                                     >  2:2518468/1‑100 (MQ=255)
             ttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCa                                                                                     >  2:5500169/1‑101 (MQ=255)
              tGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAg                                                                                    <  2:4619532/101‑1 (MQ=255)
               gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCCGA.GTTGCTCGCCCGGCAgg                                                                                   <  1:1007962/101‑1 (MQ=255)
                cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGGCCGGCAggg                                                                                  >  1:8174614/1‑101 (MQ=255)
                   aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGG.TGCTCGCCCGGCAggggcg                                                                               >  2:1846954/1‑98 (MQ=255)
                   aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGG.TGCTCGCCCGGCAGGgc.gc                                                                              >  2:6350983/1‑101 (MQ=255)
                   aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGgg.gc                                                                              >  1:2329291/1‑101 (MQ=255)
                   aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGgc.gc                                                                              >  1:1693146/1‑101 (MQ=255)
                     gggCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGG.GC..gg                                                                          >  1:2013306/1‑101 (MQ=255)
                      ggcggcGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCCGCAGGGG.GC..ggg                                                                         >  1:367376/1‑100 (MQ=255)
                       gcggcgGGCACCGCAGGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGG.TTGTCGGCCGGCCGCGC.GC..gggc                                                                        >  1:8831986/1‑99 (MQ=255)
                        cggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GC..ggggc                                                                       >  1:6893618/1‑98 (MQ=255)
                         ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GC..gggcac                                                                      <  1:8240529/101‑5 (MQ=255)
                         ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GC..gggcac                                                                      <  1:5953491/101‑5 (MQ=255)
                         ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GC..gggcac                                                                      <  2:3239952/101‑5 (MQ=255)
                          gcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GC..gggcccg                                                                     >  1:4761127/1‑96 (MQ=255)
                             ggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGCTGGCAC.GACTGGCCGA.GTTGCTCGCCCGGCAGGGC.GC..ggggcagggg                                                                  >  2:1272858/1‑93 (MQ=255)
                               cACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGG.GC..gggcgcgggcgg                                                                >  2:8679080/1‑91 (MQ=255)
                               cACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCAc.gggcgg                                                                <  1:467437/101‑5 (MQ=255)
                                  cGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGcggccc                                                              <  1:7519609/101‑1 (MQ=255)
                                   gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGcggcccg                                                             <  1:989393/101‑1 (MQ=255)
                                   gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGcggcccg                                                             <  1:8390760/101‑1 (MQ=255)
                                    cATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGcggcccgg                                                            >  2:1445590/1‑101 (MQ=255)
                                    cATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGcggcccgg                                                            <  2:91626/101‑1 (MQ=255)
                                    cATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGcggcccgg                                                            <  1:6714995/101‑1 (MQ=255)
                                      tGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGcggcccggcc                                                          <  1:3625285/101‑1 (MQ=255)
                                      tGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGcggcccggcc                                                          <  1:2811982/101‑1 (MQ=255)
                                      tGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCCGGGCCG.GGcggccccgcc                                                          >  2:9088710/1‑101 (MQ=255)
                                      cgatctcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGcggcccggcc                                                          <  2:6917509/95‑1 (MQ=255)
                                       ggCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGGCCCGCCg                                                         >  1:3249588/1‑101 (MQ=255)
                                       ggCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCg                                                         <  1:6365599/101‑1 (MQ=255)
                                         ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCGAt                                                       <  2:7268875/101‑1 (MQ=255)
                                          cgcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCGATc                                                      <  2:8831580/101‑1 (MQ=255)
                                            cgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCGATCTg                                                    <  2:7682850/101‑1 (MQ=255)
                                             gggTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCGATCTGc                                                   >  2:7411227/1‑101 (MQ=255)
                                                tCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGGGGCCCGGCCGATCCGCGGc                                                >  1:1034114/1‑101 (MQ=255)
                                                tCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCGGTCTGCGGc                                                >  1:8515453/1‑101 (MQ=255)
                                                tCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCGGTCTGCGGc                                                >  1:7967074/1‑101 (MQ=255)
                                                tCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCGATCTGCGGc                                                <  2:3386536/101‑1 (MQ=255)
                                                gcTGCCCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCGATCTGCGGc                                                <  1:3154997/100‑1 (MQ=255)
                                                 cTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCGATCTGCGGCt                                               >  1:5492031/1‑101 (MQ=255)
                                                   gACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCGGTCTGCGGCTgg                                             >  1:5705845/1‑101 (MQ=255)
                                                   gACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCGATCTGCGGCGgg                                             >  1:5341767/1‑101 (MQ=255)
                                                     ccctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCGATCTGCGGCTGGAc                                           <  2:6220503/96‑1 (MQ=255)
                                                      cctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCGATCTGCGGCTGGACg                                          <  1:1192551/97‑1 (MQ=255)
                                                       ctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGGACGGGCGGCCCGGCCGATCTGCGGCTgggccg                                         >  2:557832/4‑97 (MQ=255)
                                                       ctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCGATCTGCGGCTGGACgg                                         <  1:2375483/98‑1 (MQ=255)
                                                        tggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGGG..GGCACGGGCGGCCCGGGCGATCTGCGGcccggacgg                                        >  1:4658719/3‑93 (MQ=255)
                                                         ggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCCATCTGCGGCTGGGCGGGc                                       >  2:5892003/2‑101 (MQ=255)
                                                          gCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCggggc                                      >  1:41032/1‑99 (MQ=255)
                                                             gaggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCt                                   <  1:7555532/91‑1 (MQ=255)
                                                             gaggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCt                                   <  1:4153721/91‑1 (MQ=255)
                                                              aggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTg                                  <  1:1633198/92‑1 (MQ=255)
                                                              aggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTg                                  <  2:5970267/92‑1 (MQ=255)
                                                               ggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGa                                 <  2:3561395/93‑1 (MQ=255)
                                                               ggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGa                                 <  2:4692864/93‑1 (MQ=255)
                                                                gcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGAt                                <  1:7721580/94‑1 (MQ=255)
                                                                 cgcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGGACGGGCGGCCCGGCCGGTCTGCGGGCGGGCGGGCTCCTGATg                               >  2:7165999/7‑101 (MQ=255)
                                                                  gcagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGc                              <  1:3486766/96‑1 (MQ=255)
                                                                   cagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGcc                             <  2:6716987/97‑1 (MQ=255)
                                                                   cagctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGcc                             <  1:3325308/97‑1 (MQ=255)
                                                                    agctGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCCGCCGATCTGCGGCTGGACGGGCTCCTGGTGCCg                            >  2:1974358/4‑101 (MQ=255)
                                                                       tGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGgc                         >  2:3432102/1‑101 (MQ=255)
                                                                       tGGAACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCGACCTGCGGCTGGGCGGGGTCCTGATGCCgggg                         >  2:8243971/1‑100 (MQ=255)
                                                                        ggaacccGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGGTCCTGATGCCGGgcc                        >  2:3700581/6‑100 (MQ=255)
                                                                        ggaacccGCGATGG.CACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGGTCCTGCTGCCCGgcc                        >  2:4415705/6‑100 (MQ=255)
                                                                         gaacccGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGGTCCTGATGCCGGgcgg                       >  2:5189460/5‑100 (MQ=255)
                                                                         gaacccGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGGTCCCGGTGCCCGgggc                       >  1:927513/5‑101 (MQ=255)
                                                                         gaacccGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCCGATGCCGGgcgc                       >  1:2176518/5‑101 (MQ=255)
                                                                          aacccGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGG.GCG.GGCCCGGGCGGCCCGGCCCATCTGCGGCTGGACGGGGCCCTTATGCCCGGcgcc                      >  1:621640/4‑101 (MQ=255)
                                                                          aacccGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgcc                      <  2:8043076/98‑1 (MQ=255)
                                                                          aacccGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCCGATGCCGGGtgcc                      >  2:4246405/4‑101 (MQ=255)
                                                                          aacccGCGATGG.CACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCGATCTGCGGCTGGACCGGCTCCCGATTGCGGGcgcc                      >  2:3621795/4‑101 (MQ=255)
                                                                             ccGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCcgcg                   <  2:8515073/101‑1 (MQ=255)
                                                                             ccGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCcgcg                   <  2:1165886/101‑1 (MQ=255)
                                                                              cgcgATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGGTCCCGATGCCGGGCGCcgcgc                  >  2:1507761/1‑100 (MQ=255)
                                                                               gcgATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGAc                 <  1:7043583/101‑1 (MQ=255)
                                                                                         cggctggCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCt        <  1:4520224/95‑1 (MQ=255)
                                                                                         cggctggCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCt        <  1:1253328/95‑1 (MQ=255)
                                                                                          ggctggCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTg       <  1:8810097/96‑1 (MQ=255)
                                                                                          ggctggCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTg       <  2:2199048/96‑1 (MQ=255)
                                                                                             tggCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCCGATc    >  1:8631642/3‑101 (MQ=255)
                                                                                               gCCGA.GTTGCTCGCCCGGCAGGGC.GCG.GGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGa  >  1:7295488/1‑101 (MQ=255)
                                                                                                    |                                                                                                  
GGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGAA.CCGCGATGG.AA.GGCT.GCCGA.G.TGTTCGCCCGGCAGGGC.GC..GGCAC.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGA  >  NC_014034/3256108‑3256293

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG