Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,256,220 +G 100% coding (1363/1734 nt) recJ → single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,256,2201.→G100.0% 876.5 238coding (1363/1734 nt)recJsingle‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (93/145):  ref base (0/0):  total (93/145)

Only 100 of 311 total aligned reads displayed.

AAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGAA.CCGCGATGG.AA.GGCT.G.CCGAG.TGTTCGCCCGGCAGGGCGC.GGCA.C.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCGGCCC  >  NC_014034/3256127‑3256314
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aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGgggc                                                                                                   >  1:2329291/1‑101 (MQ=255)
aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGgcgc                                                                                                   >  1:1693146/1‑101 (MQ=255)
aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGgcgc                                                                                                   <  1:2029299/101‑1 (MQ=255)
aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGgcgc                                                                                                   <  2:1121414/101‑1 (MQ=255)
 aGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGG.CCGAGTTGCTCGCCCGGCAGGGcgc.g                                                                                                 <  1:6731448/101‑1 (MQ=255)
  gggCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGC.gg                                                                                                >  1:6189194/1‑101 (MQ=255)
   ggcggcGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGG.CCGCGTTGCTCGCCCCGCAGGGCGC.ggg                                                                                               >  2:5894973/1‑100 (MQ=255)
   ggcggcGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGGGC.ggg                                                                                               >  1:3631679/1‑100 (MQ=255)
   ggcggcGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCCGCAGGGGGC.ggg                                                                                               >  1:367376/1‑100 (MQ=255)
     cggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGC.gggca                                                                                             <  2:6188955/101‑4 (MQ=255)
      ggcggGCGCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGC.gggcac                                                                                            <  1:4120857/101‑5 (MQ=255)
      ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGGGC.ggggag                                                                                            >  2:5545685/1‑97 (MQ=255)
      ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGC.ggggac                                                                                            >  1:9174802/1‑97 (MQ=255)
      ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGC.gggcgc                                                                                            >  2:1503573/1‑97 (MQ=255)
      ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGC.gggcac                                                                                            >  1:8923875/1‑97 (MQ=255)
      ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGC.gggcac                                                                                            <  1:8240529/101‑5 (MQ=255)
      ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGC.gggcac                                                                                            <  1:6879810/101‑5 (MQ=255)
        cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.c.gg                                                                                         <  1:8632955/101‑1 (MQ=255)
        cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.c.gg                                                                                         <  2:8187493/101‑1 (MQ=255)
          ggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCCGGGCGCGGGCA.c.gggg                                                                                       >  1:4185720/1‑99 (MQ=255)
           gCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.c.gggcg                                                                                      <  2:650420/101‑4 (MQ=255)
             aCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.c.gggcggc                                                                                    >  1:7434105/1‑96 (MQ=255)
                gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGcggcccg                                                                                  <  2:737034/101‑1 (MQ=255)
                 cATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCCGCAGGGCGCGGGGA.CGGGcggcccgg                                                                                 >  2:3585030/1‑101 (MQ=255)
                  aTGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGcggcccggc                                                                                >  1:1021960/1‑101 (MQ=255)
                   tGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGcggcccggcc                                                                               <  1:2811982/101‑1 (MQ=255)
                    ggCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCg                                                                              <  1:5867581/101‑1 (MQ=255)
                     gCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCACC.GGGGGGCCGGCCgg                                                                             >  2:8812268/1‑100 (MQ=255)
                      ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGAt                                                                            <  2:3711334/101‑1 (MQ=255)
                      ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGAt                                                                            <  2:7268875/101‑1 (MQ=255)
                        gcgGGTCGGACCCTGGCCGAGGCGAAGCTGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCt                                                                          <  1:5469316/101‑1 (MQ=255)
                         cgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGcggcccggccagatcg                                                                         >  1:6917794/1‑95 (MQ=255)
                         cgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTg                                                                         <  2:9130337/101‑1 (MQ=255)
                          gggTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGGGGCCCGGCCGATCTGc                                                                        >  1:111214/1‑101 (MQ=255)
                           ggTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCg                                                                       <  2:6304843/101‑1 (MQ=255)
                            gTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCgg                                                                      >  2:8592640/1‑101 (MQ=255)
                              cTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCt                                                                    <  1:7622672/101‑1 (MQ=255)
                              cTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCt                                                                    <  1:4308287/101‑1 (MQ=255)
                               tGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTg                                                                   <  2:8861345/101‑1 (MQ=255)
                                gACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCTCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTgg                                                                  <  1:4785321/101‑1 (MQ=255)
                                gACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGGTCTGCGGCTgg                                                                  >  1:5705845/1‑101 (MQ=255)
                                  ccctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGAc                                                                <  2:1649731/96‑1 (MQ=255)
                                    ctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACgg                                                              <  1:2375483/98‑1 (MQ=255)
                                      ggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCgggg                                                            >  1:3404501/2‑100 (MQ=255)
                                           aggcgcagctGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTg                                                       <  2:5970267/92‑1 (MQ=255)
                                           aggcgcagctGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTg                                                       <  1:1633198/92‑1 (MQ=255)
                                            ggcgcagctGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGa                                                      <  2:4692864/93‑1 (MQ=255)
                                            ggcgcagctGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGa                                                      <  1:7531224/93‑1 (MQ=255)
                                            ggcgcagctGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGa                                                      <  2:367368/93‑1 (MQ=255)
                                             gcgcagctGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGAt                                                     <  1:6312857/94‑1 (MQ=255)
                                              cgcagctGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATg                                                    <  2:3465322/95‑1 (MQ=255)
                                              cgcagctGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCCGGACGGGCGCCCGATg                                                    >  1:7073006/7‑101 (MQ=255)
                                               gcagctGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGc                                                   <  2:6770616/96‑1 (MQ=255)
                                               gcagctGGAACCCGCGATGGCAC.GGCT.GCCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGc                                                   <  1:6400575/96‑1 (MQ=255)
                                                cagctGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGcc                                                  <  1:3120351/97‑1 (MQ=255)
                                                 agctGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGGTCCCGATGCCg                                                 >  1:98479/4‑101 (MQ=255)
                                                 agctGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCCGCCGATCTGCGGCTGGACGGGCTCCTGGTGCCg                                                 >  2:1974358/4‑101 (MQ=255)
                                                 agctGGAACCCGCGATGG.CACGGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCGGGGACGGCTCCCGGTGCCg                                                 >  1:1206592/4‑101 (MQ=255)
                                                  gctGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCCGATGCCgg                                                >  1:8496285/3‑101 (MQ=255)
                                                    tGGAACCCGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGgc                                              >  1:4651004/1‑101 (MQ=255)
                                                     ggaacccGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGGTCCTGATGCCggggg                                             >  2:1316151/6‑99 (MQ=255)
                                                     ggaacccGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACCGGGTCCTGATGCCCGgcg                                             >  1:357266/6‑101 (MQ=255)
                                                     ggaacccGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCCGGACGGGCTCCTGGTGCCGGgcg                                             >  1:451332/6‑101 (MQ=255)
                                                      gaacccGCGATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGGTCCCGGTGCCCGgggc                                            >  1:927513/5‑101 (MQ=255)
                                                       aacccGCGATGG.CACGGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACCGGCTCCCGATTGCGGGcgcc                                           >  2:3621795/4‑101 (MQ=255)
                                                            gcgATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGAc                                      <  1:4666333/101‑1 (MQ=255)
                                                            gcgATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGAc                                      <  2:2458948/101‑1 (MQ=255)
                                                            gcgATGGCAC.GGCTGG.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGAc                                      <  2:1873223/101‑1 (MQ=255)
                                                                  ggcacggctgg.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCcg                                 <  2:3386657/91‑1 (MQ=255)
                                                                      cggctgg.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCCGATGCCGGGCGCCGCGACGCCCgcgcc                             >  1:8543398/7‑100 (MQ=255)
                                                                       ggctgg.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTg                            <  2:2199048/96‑1 (MQ=255)
                                                                       ggctgg.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTg                            >  2:8724203/6‑101 (MQ=255)
                                                                       ggctgg.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTg                            <  1:8810097/96‑1 (MQ=255)
                                                                         ctgg.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGAt                          <  2:7919398/98‑1 (MQ=255)
                                                                           gg.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCg                        >  2:7515938/2‑101 (MQ=255)
                                                                           gg.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCg                        <  1:1282070/100‑1 (MQ=255)
                                                                           gg.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCg                        <  2:2722368/100‑1 (MQ=255)
                                                                            g.CCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGa                       >  1:5303293/1‑101 (MQ=255)
                                                                               cgagttgctCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGAGGCCGGGGCCCGCGGCGCCCGCGCTGAGCGAgg                     >  2:4254737/9‑101 (MQ=255)
                                                                                gagttgctCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGa                    >  1:1392924/8‑101 (MQ=255)
                                                                                 agttgctCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGAt                   <  1:3986260/95‑1 (MQ=255)
                                                                                  gttgctCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGAtc                  <  1:1889501/96‑1 (MQ=255)
                                                                                   ttgctCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGAtct                 <  2:5385016/97‑1 (MQ=255)
                                                                                   ttgctCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGAtct                 <  2:41032/97‑1 (MQ=255)
                                                                                    tgctCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGAtctc                >  2:9168521/4‑101 (MQ=255)
                                                                                    tgctCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGAtctc                <  2:316390/98‑1 (MQ=255)
                                                                                      ctCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGa              <  1:3204227/100‑1 (MQ=255)
                                                                                        cGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGAcc            <  2:4185602/101‑1 (MQ=255)
                                                                                        cGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGAcc            <  1:2135961/101‑1 (MQ=255)
                                                                                        cGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGAcc            >  2:1187453/1‑101 (MQ=255)
                                                                                        cGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGAcc            >  2:844447/1‑101 (MQ=255)
                                                                                        cGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCCAcc            <  1:7196625/101‑1 (MQ=255)
                                                                                         gCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACcg           <  2:717129/101‑1 (MQ=255)
                                                                                         gCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACcg           <  1:7965126/101‑1 (MQ=255)
                                                                                              gCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCg      >  1:8049666/1‑101 (MQ=255)
                                                                                              gCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCg      <  2:5705638/101‑1 (MQ=255)
                                                                                              gCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCg      <  2:5091840/101‑1 (MQ=255)
                                                                                               cAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCgg     >  2:4622612/1‑101 (MQ=255)
                                                                                                aGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCGGc    >  2:6046519/1‑101 (MQ=255)
                                                                                                  ggCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGTGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCGGccc  <  2:7057273/101‑1 (MQ=255)
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AAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGAA.CCGCGATGG.AA.GGCT.G.CCGAG.TGTTCGCCCGGCAGGGCGC.GGCA.C.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCGGCCC  >  NC_014034/3256127‑3256314

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG