BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,258,963	+C	100%	coding (778/1971 nt)	efeU →	ferrous iron permease EfeU

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,258,963	1	.→C	100.0%	81.9	388	coding (778/1971 nt)	efeU	ferrous iron permease EfeU
Reads supporting (aligned to +/- strand): new base (203/156): ref base (1/28): total (204/184)
Fisher's exact test strand distribution p-value = 7.34e-09
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.29e-03

Only 100 of 435 total aligned reads displayed.


CAATGCCTCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.A.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGGCGCCTCCTT  >  NC_014034/3258863‑3259058


cAATGCCTCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.a                                                                                                  >  1:9028171/1‑101 (MQ=255)


cAATGCCTCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.a                                                                                                  >  2:1625597/1‑101 (MQ=255)


 aaTGCCTCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.A.c                                                                                                <  1:4330811/101‑1 (MQ=255)


  aTGCCTCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.A.cc                                                                                               >  1:8620090/1‑101 (MQ=255)


  aTGCCTCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.A.cc                                                                                               <  2:7592989/101‑1 (MQ=255)


    gCCTCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.A.ccct                                                                                             <  1:1287319/101‑3 (MQ=255)


    gCCTCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.A.ccct                                                                                             <  1:2955822/101‑3 (MQ=255)


     ccTCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.A.ccctg                                                                                            >  2:715459/1‑98 (MQ=255)


         ggTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGc                                                                                         >  1:6915094/1‑101 (MQ=255)


            cgcgGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGctct                                                                                      >  2:4664884/1‑101 (MQ=255)


            cgcgGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGctct                                                                                      >  2:3817943/1‑101 (MQ=255)


             gcgGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACACCCCGACCAGGGGCGAGGTCACCCTTTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCtt                                                                                     <  1:1625644/101‑1 (MQ=255)


              cgGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTc                                                                                    >  1:6242104/1‑101 (MQ=255)


                ggCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCt                                                                                  <  1:7636779/101‑1 (MQ=255)


                ggCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCt                                                                                  <  2:1801331/101‑1 (MQ=255)


                ggCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCt                                                                                  <  2:3128085/101‑1 (MQ=255)


                ggCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCt                                                                                  >  2:3651279/1‑101 (MQ=255)


                 gCACCGTGCTTTCGGCGCTCTTTGGCTATCACTACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTg                                                                                 <  2:5010517/101‑1 (MQ=255)


                   aCCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTgcg                                                                               >  1:4869386/1‑101 (MQ=255)


                   aCCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTgcg                                                                               <  2:3613110/101‑1 (MQ=255)


                   aCCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTgcg                                                                               >  2:6639524/1‑101 (MQ=255)


                     cGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGcc                                                                             >  1:8724922/1‑101 (MQ=255)


                       tGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGcccc                                                                           >  1:1936449/1‑101 (MQ=255)


                       tGCTTGCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGcccc                                                                           <  1:4153705/101‑1 (MQ=255)


                        gCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGcccgg                                                                          >  2:263741/1‑99 (MQ=255)


                        gCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCcg                                                                          >  1:3152158/1‑101 (MQ=255)


                         cTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCcgc                                                                         >  1:8921115/1‑101 (MQ=255)


                          tttCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCcgcg                                                                        <  2:5839895/101‑1 (MQ=255)


                             cGGCGCTCTTTGGCTATCACGACAGCCCGCCCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTC.ACCCTGCCGCTCTTCCTGCGCCCCGcgccc                                                                     <  2:248391/101‑1 (MQ=255)


                             cGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGcgccc                                                                     <  2:4059991/101‑1 (MQ=255)


                               gcgcTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGcgcccgc                                                                   >  1:1750166/1‑101 (MQ=255)


                                cgcTCTTTGGCTATCACGACAGCCCGACCCCGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGcgcccgcc                                                                  <  1:643185/101‑1 (MQ=255)


                                cgcTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGcgcccgcc                                                                  <  1:2298676/101‑1 (MQ=255)


                                 gcTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCgcccgccc                                                                 <  2:2874758/101‑1 (MQ=255)


                                   tctTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGccccc                                                               >  1:2810798/1‑101 (MQ=255)


                                    ctTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGcccccc                                                              >  2:8495115/1‑101 (MQ=255)


                                     ttgggCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCt                                                             <  1:3717833/98‑1 (MQ=255)


                                       tGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTc                                                           >  2:7385441/1‑101 (MQ=255)


                                       tGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCAGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTc                                                           >  1:6110387/1‑101 (MQ=255)


                                        ggCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTcc                                                          <  1:7261219/101‑1 (MQ=255)


                                        ggCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTcc                                                          <  1:6013556/101‑1 (MQ=255)


                                           tATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGACCCCCTTCCGCa                                                       >  2:5924201/1‑101 (MQ=255)


                                            aTCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCaa                                                      >  2:2773115/1‑101 (MQ=255)


                                            aTCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCaa                                                      <  2:9084676/101‑1 (MQ=255)


                                             tCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCaaa                                                     >  2:8019198/1‑101 (MQ=255)


                                              cACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCaaaa                                                    >  1:2680771/1‑101 (MQ=255)


                                              cACCACAGCCCGACCCGGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCaaaa                                                    <  2:6220313/101‑1 (MQ=255)


                                               aCGACAGCCCGGCCCAGGGCGAGGTCGCCCTCTGGGCCGCTTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAg                                                   <  1:1068619/101‑1 (MQ=255)


                                               aCGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAg                                                   <  1:6735660/101‑1 (MQ=255)


                                                   caccccGCCCCAGGGCGAGGTCCCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCt                                               <  2:2982201/98‑1 (MQ=255)


                                                   cAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCGCCCTTCCCCACACGGccg                                               >  2:4254710/1‑100 (MQ=255)


                                                    aGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTg                                              <  1:7521397/101‑1 (MQ=255)


                                                     gCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGa                                             <  2:8318155/101‑1 (MQ=255)


                                                      cccGCCCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGAt                                            <  2:2912448/101‑1 (MQ=255)


                                                       ccGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATc                                           >  2:7903638/1‑101 (MQ=255)


                                                       ccGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATc                                           <  1:4626900/101‑1 (MQ=255)


                                                        cGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATcc                                          <  1:2089868/101‑1 (MQ=255)


                                                        cGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATcc                                          <  1:5178243/101‑1 (MQ=255)


                                                         gACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCg                                         <  1:7861699/101‑1 (MQ=255)


                                                           cccAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGAt                                       >  1:4018200/1‑101 (MQ=255)


                                                            ccAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATg                                      <  1:2023726/101‑1 (MQ=255)


                                                            ccAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATg                                      <  2:689790/101‑1 (MQ=255)


                                                             cAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGa                                     <  2:86346/101‑1 (MQ=255)


                                                             cAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGa                                     <  1:2393838/101‑1 (MQ=255)


                                                              aGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGAc                                    >  1:5364097/1‑101 (MQ=255)


                                                                ggCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGAccc                                  >  2:6406732/1‑101 (MQ=255)


                                                                  cGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCt                                >  2:5649478/1‑101 (MQ=255)


                                                                   gAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCtt                               >  2:3996785/1‑101 (MQ=255)


                                                                   gAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCtt                               <  1:5733083/101‑1 (MQ=255)


                                                                   gAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCtt                               >  2:3425114/1‑101 (MQ=255)


                                                                   gAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCtt                               >  2:2351922/1‑101 (MQ=255)


                                                                   gAGGTCGCCCTCGGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCtt                               <  2:6102075/101‑1 (MQ=255)


                                                                    aGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTc                              <  2:2081264/101‑1 (MQ=255)


                                                                    aGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTc                              >  1:8273021/1‑101 (MQ=255)


                                                                     ggTCCCCCTCTGGGCCGCGTTCCTGGCCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTcc                             <  1:794066/101‑1 (MQ=255)


                                                                      gTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCg                            <  2:1045607/101‑1 (MQ=255)


                                                                       gcGCCCTCTGGCCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGc                           <  2:8768106/100‑1 (MQ=255)


                                                                        cGCCCTCTGGGCCGCGTTCCTGGCCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGca                          <  1:8108265/101‑1 (MQ=255)


                                                                         gCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTTCCGCTCTTCCTGCGCCCCGCGCCCGACCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGcac                         <  2:4454003/101‑1 (MQ=255)


                                                                           ccTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACaa                       <  1:4467204/101‑1 (MQ=255)


                                                                            ctctGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAg                      >  2:7669635/1‑101 (MQ=255)


                                                                            ctctGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAg                      >  1:2150993/1‑101 (MQ=255)


                                                                             tctGGGCCGCGTTCCTGGCCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAgc                     <  1:254732/101‑1 (MQ=255)


                                                                             tctGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAgc                     >  2:8084198/1‑101 (MQ=255)


                                                                             tctGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAgc                     <  1:3381207/101‑1 (MQ=255)


                                                                              ctGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAgcg                    >  2:5243814/1‑101 (MQ=255)


                                                                               tGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAgcgc                   >  1:8323192/1‑101 (MQ=255)


                                                                                gggCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGcc                  <  1:5846504/101‑1 (MQ=255)


                                                                                 ggCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCg                 >  2:5757422/1‑101 (MQ=255)


                                                                                  gCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGt                <  2:1332935/101‑1 (MQ=255)


                                                                                   ccGCGTTCCTGGCCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTc               <  2:4854513/101‑1 (MQ=255)


                                                                                    cgcgTTCCTGGCCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTcc              <  1:3635714/101‑1 (MQ=255)


                                                                                     ccgTTCCTGGCGGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCg             <  1:1565793/100‑1 (MQ=255)


                                                                                        ttCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGgc          >  1:6497015/1‑101 (MQ=255)


                                                                                        ttCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGgc          >  1:922330/1‑101 (MQ=255)


                                                                                            tGGCCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGGCGcct      <  2:5424963/101‑1 (MQ=255)


                                                                                             ggCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGGCGcctc     >  2:8254073/1‑101 (MQ=255)


                                                                                             ggCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGGCGcctc     >  1:7167923/1‑101 (MQ=255)


                                                                                             ggCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGGCGcctc     >  2:1958539/1‑101 (MQ=255)


                                                                                                cGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGGCGCCTCCtt  >  2:3292634/1‑101 (MQ=255)


CAATGCCTCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.A.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGGCGCCTCCTT  >  NC_014034/3258863‑3259058

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 41 ≤


ATCG