Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,532,987 +G 91.4% intergenic (‑6/‑19) RCAP_rcc03340 ← / → RCAP_rcc03341 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,532,9871.→G91.4% 96.3 ‑0.5 ‑0.8385intergenic (‑6/‑19)RCAP_rcc03340/RCAP_rcc03341hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (172/180):  ref base (14/19):  total (186/199)
Fisher's exact test strand distribution p-value = 5.85e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.48e-01

Only 100 of 420 total aligned reads displayed.

AGCGGGGAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTC.GG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.C.GG.CC.C.GCCCTTCGATCCAGTGCAGCCAACCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.CTGCGCGCCCGGCAGGGCCGCCAGATCGTGC  >  NC_014034/3532888‑3533080
                                                                                                       |                                                                                                 
aGCGGGGAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.c                                                                                                    >  2:7164630/1‑101 (MQ=255)
aGCGGGGAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.c                                                                                                    >  1:2012428/1‑101 (MQ=255)
 gCGGGGAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.C.g                                                                                                  <  2:6266816/101‑1 (MQ=255)
  cGGGGAAAGCGGAGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.C.gg                                                                                                 >  2:8830213/1‑101 (MQ=255)
   ggggAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.C.ggg                                                                                                >  1:6015768/1‑100 (MQ=255)
    gggAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.C.gggc                                                                                               >  1:322700/1‑99 (MQ=255)
     ggAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.C.Gg.gc.c                                                                                            <  2:6704092/101‑1 (MQ=255)
      gAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.C.Gg.gc.cc                                                                                           >  2:489333/1‑100 (MQ=255)
       aaggcggcgCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.C.Gg.gc.ccg                                                                                          <  2:6861772/98‑3 (MQ=255)
       aaaGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.C.Gg.gc.ccg                                                                                          <  1:4424879/101‑3 (MQ=255)
         aGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.C.Gg.gc.ccggc                                                                                        <  1:2850886/101‑5 (MQ=255)
           cggcgCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.C.Gg.gc.ccggccc                                                                                      <  2:8598333/101‑7 (MQ=255)
           cggcgCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.C.Gg.gc.ccggccc                                                                                      >  2:3648177/1‑95 (MQ=255)
            ggcgCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.C.Gg.gc.ccggccct                                                                                     >  2:4579700/1‑94 (MQ=255)
              cgcCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTc                                                                                    >  2:3211067/1‑101 (MQ=255)
               gcCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCg                                                                                   >  1:6324590/1‑101 (MQ=255)
               gcCTGGCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCg                                                                                   <  1:2989570/101‑1 (MQ=255)
                 cTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGAt                                                                                 <  1:7144943/101‑1 (MQ=255)
                 cTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGAt                                                                                 <  1:415857/101‑1 (MQ=255)
                   gtgggTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATcc                                                                               <  1:6812915/98‑1 (MQ=255)
                   gTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATcc                                                                               >  1:8004039/1‑101 (MQ=255)
                     cGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAg                                                                             <  2:2505050/101‑1 (MQ=255)
                     cGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAg                                                                             >  2:4472353/1‑101 (MQ=255)
                     cGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAg                                                                             <  1:3635817/101‑1 (MQ=255)
                     cGGCCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAg                                                                             <  2:5398754/101‑1 (MQ=255)
                      ggTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGt                                                                            >  2:3126774/1‑101 (MQ=255)
                      ggTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGt                                                                            >  1:6677697/1‑101 (MQ=255)
                         ccAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCa                                                                         >  1:5833082/1‑101 (MQ=255)
                            gCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGcc                                                                      <  2:5346479/101‑1 (MQ=255)
                            gCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGcc                                                                      <  2:568340/101‑1 (MQ=255)
                               gaccgaAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCagc                                                                   <  2:1173612/101‑3 (MQ=255)
                               gaccgaAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.C.GGACC.CGGCCCTTCGATCCAGTGCAGCCagc                                                                   >  1:6394335/1‑99 (MQ=255)
                                accgaAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGG.GGCGATCGGCGCCATCCCGGCCCACAGCCG.CTGG.CCGG.GCCCTTCGCTCCAGTCCAGcagggc                                                                  >  2:3459676/1‑96 (MQ=255)
                                  cgaAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCgc                                                                >  1:1070959/1‑100 (MQ=255)
                                   gaAAGCAGCCGCGCCGCGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTc                                                               <  2:7615862/101‑1 (MQ=255)
                                    aaaGCAGCCGCGCCTCGGCCTGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCTTcc                                                              <  1:7203212/101‑1 (MQ=255)
                                    aaaGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTcc                                                              >  2:7495114/1‑101 (MQ=255)
                                    aaaGCAGCCGCGCCTCGACCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTcc                                                              >  2:1438719/1‑101 (MQ=255)
                                     aaGCAGCCGCGCCTCGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCt                                                             >  2:8536879/1‑101 (MQ=255)
                                      agcagcCGCGCCTCGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTc                                                            <  2:6394086/101‑1 (MQ=255)
                                       gcagcCGCGCCTCGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCg                                                           <  2:5202155/101‑1 (MQ=255)
                                        cagcCGCGCCTCGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGa                                                          <  2:6015533/101‑1 (MQ=255)
                                         agcCGCGCCTCGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGAc                                                         >  2:7941515/1‑101 (MQ=255)
                                          gcCGCGCCTCGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGAcc                                                        <  1:6587803/101‑1 (MQ=255)
                                          gcCGCGCCTCGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGAcc                                                        <  2:8159175/101‑1 (MQ=255)
                                             gcgcCTCGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCa                                                     <  1:3126873/101‑1 (MQ=255)
                                              cgcCTCGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCaa                                                    <  1:4869586/101‑1 (MQ=255)
                                                ccTCGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAAcc                                                  >  2:4396981/1‑101 (MQ=255)
                                                ccTCGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAAcc                                                  <  1:258576/101‑1 (MQ=255)
                                                   cGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTc                                               <  2:4733789/101‑1 (MQ=255)
                                                   cGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTc                                               >  1:5560487/1‑101 (MQ=255)
                                                   cGGCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCCTCCTCGACCGCCACCGTc                                               >  2:515531/1‑101 (MQ=255)
                                                    ggCCAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCg                                              >  2:7269068/1‑101 (MQ=255)
                                                    ggCCAGCCGGGGGTCGGG.GGCGATCTGCGCCACCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCg                                              >  2:7677968/1‑101 (MQ=255)
                                                      ccAGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGcc                                            <  1:9097452/101‑1 (MQ=255)
                                                        aGCCGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGcccc                                          <  1:8352192/101‑1 (MQ=255)
                                                          ccGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGc                                        <  2:8789965/101‑1 (MQ=255)
                                                          ccGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGc                                        >  1:4704151/1‑101 (MQ=255)
                                                           cGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGca                                       >  2:1251021/1‑101 (MQ=255)
                                                           cGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGca                                       >  2:6980580/1‑101 (MQ=255)
                                                           cGGGGGTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGca                                       <  1:4029045/101‑1 (MQ=255)
                                                            gggggTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGcac                                      <  2:2012367/101‑1 (MQ=255)
                                                            gggggTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGcac                                      >  1:8298005/1‑101 (MQ=255)
                                                             ggggTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGcaca                                     <  2:5832847/101‑1 (MQ=255)
                                                             ggggTCGGG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGcaca                                     <  2:7800551/101‑1 (MQ=255)
                                                               ggTC.GGAGGCGATCTGCGCCATCGCGGCGCAAACCCGCC.GG.CC.CGGCCCTTCGATCCAGCCCACCCAGCCGTCCTCGACCGCAACCTTCGCCCCGCACAGa                                   <  1:343507/101‑1 (MQ=255)
                                                                gtcggg.ggCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAt                                  <  1:5126900/97‑1 (MQ=255)
                                                                  cggg.ggCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.cc                               <  1:4072705/99‑2 (MQ=255)
                                                                   ggg.ggCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.cct                              <  1:3091255/100‑3 (MQ=255)
                                                                   ggg.ggCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.cct                              <  2:901303/100‑3 (MQ=255)
                                                                   ggg.ggCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.cct                              <  1:1463018/100‑3 (MQ=255)
                                                                   ggg.ggCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.cct                              >  2:1763017/2‑99 (MQ=255)
                                                                   ggg.ggCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.cct                              >  1:7014884/2‑99 (MQ=255)
                                                                   ggg.ggCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.cct                              >  1:3824814/2‑99 (MQ=255)
                                                                       ggCGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.cctgcg                           >  2:2783752/1‑96 (MQ=255)
                                                                         cGATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTgcgcg                          >  2:8250144/1‑101 (MQ=255)
                                                                          gATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTgcgcgc                         >  1:812082/1‑101 (MQ=255)
                                                                          gATCTGCGCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTgcgcgc                         <  1:887050/101‑1 (MQ=255)
                                                                            tCTGCTCCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGccc                       <  1:7450390/101‑1 (MQ=255)
                                                                               gcgcCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGc                    <  1:5337934/101‑1 (MQ=255)
                                                                                cgcCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGcccagat                   >  1:7637596/1‑97 (MQ=255)
                                                                                 gcCATCGCGGCGCAAAGCTT.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAg                  >  1:2442505/1‑101 (MQ=255)
                                                                                 gcCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAg                  <  1:7112208/101‑1 (MQ=255)
                                                                                 gcCATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAg                  >  2:415068/1‑101 (MQ=255)
                                                                                  ccATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGCTCCTGCGCGCCCGGCCgg                 >  1:851050/1‑101 (MQ=255)
                                                                                   cATCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAggg                <  2:2346351/101‑1 (MQ=255)
                                                                                    aTCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGgc               >  2:8047046/1‑101 (MQ=255)
                                                                                     tCGCGGCGCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGgcc              <  2:3866345/101‑1 (MQ=255)
                                                                                        cggcgCAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGgccgcc           <  2:1496708/101‑1 (MQ=255)
                                                                                          gcgcAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGGCCGCCAg         <  1:4579835/101‑1 (MQ=255)
                                                                                            gcAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGGCCGCCAGAt       >  1:5737938/1‑101 (MQ=255)
                                                                                            ccAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGGCCGCCAGAt       <  2:1548930/100‑1 (MQ=255)
                                                                                             cAAAGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGGCCGCCAGATc      >  1:4786912/1‑101 (MQ=255)
                                                                                              aaaGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGCCCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGGCCGCCACATCg     >  2:2955273/1‑101 (MQ=255)
                                                                                              aaaGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGGCCGCCAGATCg     <  1:7848321/101‑1 (MQ=255)
                                                                                              aaaGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGGCCGCCAGATCg     >  1:4903521/1‑101 (MQ=255)
                                                                                              aaaGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGGCCGCCAGATCg     >  1:1627525/1‑101 (MQ=255)
                                                                                              aaaGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGGCCGCCAGATCg     >  1:2649097/1‑101 (MQ=255)
                                                                                              aaaGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGGCCGCCAGATCg     >  2:40163/1‑101 (MQ=255)
                                                                                               aaGCTG.CGGG.CC.CGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGGCCGCCAGATCGt    >  1:1815102/1‑101 (MQ=255)
                                                                                                       |                                                                                                 
AGCGGGGAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTC.GG.GGCGATCTGCGCCATCGCGGCGCAAAGCTG.C.GG.CC.C.GCCCTTCGATCCAGTGCAGCCAACCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.CTGCGCGCCCGGCAGGGCCGCCAGATCGTGC  >  NC_014034/3532888‑3533080

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG