BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,560,922	+G	98.9%	coding (330/1134 nt)	bioF →	8‑amino‑7‑oxononanoate synthase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,560,922	1	.→G	98.9%	3.4 ‑1.0 ‑0.2	370	coding (330/1134 nt)	bioF	8‑amino‑7‑oxononanoate synthase
Reads supporting (aligned to +/- strand): new base (185/181): ref base (1/3): total (186/184)
Fisher's exact test strand distribution p-value = 3.70e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.33e-01

Only 100 of 439 total aligned reads displayed.


CTGGAGCAAGAGGCCGCCGGTTTTTTCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCTCTTGCGACGCTGCCGAT.GGC.GG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAGGCACGCAGCTTTCGCCACAATGA  >  NC_014034/3560821‑3561017


cTGGAGCAAGAGGCCGCCGGTTTTTTCGGCGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGc                                                                                                    >  1:240685/1‑101 (MQ=255)


 tGGAGCAAGAGGCCGCCGGTTTTTTCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGC.g                                                                                                  >  1:1051264/1‑101 (MQ=255)


 tGGAGCAAGAGGCCGCCGGTTTTTTCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGC.g                                                                                                  >  1:1603543/1‑101 (MQ=255)


 tGGAGCAAGAGGCCGCCGGTTTTTTCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGC.g                                                                                                  >  2:8325837/1‑101 (MQ=255)


   gAGCAAGAGGCCGCCGGTTTTTTCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGC.gg.g                                                                                               >  2:3835896/1‑101 (MQ=255)


    aGCAAGAGGCCGCCGGTTTTTTCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGC.gg.g.g                                                                                             >  2:3929677/1‑101 (MQ=255)


     gCAAGAGGCCGCCGGTTTTTTCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGC.gg.g.gg                                                                                            <  1:8442983/101‑2 (MQ=255)


     gCAAGAGGCCGCCGGTTTTTTCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGC.gg.g.gg                                                                                            <  1:8509690/101‑2 (MQ=255)


      cAAGAGGCCGCCGGTTTTTTCGGTGCCTAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGC.gg.g.gga                                                                                           >  1:8644792/1‑99 (MQ=255)


      cAAGAGGCCGCCGGTTTTTTCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGC.gg.g.ggg                                                                                           >  2:6780254/1‑99 (MQ=255)


       aaGAGGCCGCCGGTTTTTTCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGCT.GGC.gg.g.ggat                                                                                          >  1:1850903/1‑98 (MQ=255)


       aaGAGGCCGCCGGTTTTTTCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGC.gg.g.ggat                                                                                          <  1:4155113/101‑4 (MQ=255)


        agagGCCGCCTGTTTTTTCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGC.gg.g.ggatc                                                                                         >  1:3610993/1‑97 (MQ=255)


         gagGCCGCCGGTTTTTTCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGC.gg.g.ggatct                                                                                        <  1:6327565/101‑6 (MQ=255)


          gggCCGCCGGTTTTTTCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTg                                                                                        <  1:2626605/100‑1 (MQ=255)


          agGCCGCCGGTTTTTGCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTg                                                                                        <  1:1641856/101‑1 (MQ=255)


            gccgccGGTTTTTTCGGTGCCGAGGCCTGTCTTTTCATTGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGATGGGG.GG.G.GATCTGGt                                                                                      <  1:6563543/101‑1 (MQ=255)


               gccgGTTTTTTCGGTGCTGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATGAGGCGGTCT.TTGCGACGCTGCCGAT.GGC.GGCG.GATCTGGtact                                                                                   <  2:765292/101‑1 (MQ=255)


                 cgGTTTTTTCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGtgctgc                                                                                 >  2:5252392/1‑101 (MQ=255)


                 cgGTTTTTTCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGtgctgc                                                                                 >  2:6425559/1‑101 (MQ=255)


                  ggTTTTTTCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGTTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCa                                                                                >  1:316023/1‑101 (MQ=38)


                  ggTTTTTTCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCa                                                                                >  1:8897635/1‑101 (MQ=255)


                  ggTTTTTTCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCa                                                                                >  2:5587407/1‑101 (MQ=255)


                   gTTTTTTCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCAt                                                                               <  2:1051246/101‑1 (MQ=255)


                    ttttttCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATg                                                                              <  2:1716792/101‑1 (MQ=255)


                    ttttttCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATg                                                                              <  1:5259882/101‑1 (MQ=255)


                    ttttttCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATg                                                                              >  1:5555552/1‑101 (MQ=255)


                    ttttttCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATg                                                                              >  2:3501498/1‑101 (MQ=255)


                      ttttCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGGGCTGCATGAc                                                                            >  2:6592295/1‑101 (MQ=255)


                        ttCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGt                                                                          >  1:5352693/1‑101 (MQ=255)


                         tCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTg                                                                         >  1:5178006/1‑101 (MQ=255)


                          cGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGc                                                                        >  2:2350974/1‑101 (MQ=255)


                           ggTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCt                                                                       >  1:7440061/1‑101 (MQ=255)


                             tGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGa                                                                     >  1:5794904/1‑101 (MQ=255)


                              gCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGAt                                                                    <  2:8447297/101‑1 (MQ=255)


                                 gAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCa                                                                 >  2:6944863/1‑101 (MQ=255)


                                 gAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCa                                                                 >  1:3943572/1‑101 (MQ=255)


                                 gAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCAAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCa                                                                 <  1:4342222/101‑1 (MQ=255)


                                  aGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCAt                                                                <  2:246201/101‑1 (MQ=255)


                                  aGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCAt                                                                <  1:7606282/101‑1 (MQ=255)


                                   ggCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATg                                                               <  1:509611/101‑1 (MQ=255)


                                   ggCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATg                                                               <  2:2759952/101‑1 (MQ=255)


                                    gCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGc                                                              >  2:1630528/1‑101 (MQ=255)


                                    gCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGc                                                              >  2:1926214/1‑101 (MQ=255)


                                    gCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGc                                                              <  2:3571566/101‑1 (MQ=255)


                                    gCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGc                                                              >  1:6050264/1‑101 (MQ=255)


                                    gCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGC.GG.GCGATCTGGTGCTGCATGACGTGCTGATCCATGc                                                              <  1:6990567/101‑1 (MQ=255)


                                     ccTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGcc                                                             <  2:7244915/101‑1 (MQ=255)


                                      cTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCa                                                            >  2:4766582/1‑101 (MQ=255)


                                        gTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAgc                                                          <  1:4041726/101‑1 (MQ=255)


                                           ttttCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAgcgcg                                                       >  1:4656412/1‑101 (MQ=255)


                                              tCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCAt                                                    >  1:4804053/1‑101 (MQ=255)


                                               cATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATg                                                   >  2:8816386/1‑101 (MQ=255)


                                               cATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATg                                                   >  1:2558478/1‑101 (MQ=255)


                                               cATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATg                                                   <  1:1336651/101‑1 (MQ=255)


                                                aTGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGa                                                  >  1:273800/1‑101 (MQ=255)


                                                aTGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGGGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGa                                                  >  2:3153397/1‑101 (MQ=255)


                                                   ggcggcGGGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAggg                                               <  1:2992845/101‑1 (MQ=255)


                                                     cggcggGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGc                                             <  2:7298877/101‑1 (MQ=255)


                                                       gcggGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTg                                           <  1:7708949/101‑1 (MQ=255)


                                                        cggGTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGc                                          <  1:3539635/101‑1 (MQ=255)


                                                         gggTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCg                                         <  1:5615996/101‑1 (MQ=255)


                                                         gggTTCCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCg                                         <  1:8170007/101‑1 (MQ=255)


                                                          ggTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCgg                                        <  2:4528657/101‑1 (MQ=255)


                                                          ggTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCgg                                        >  1:840526/1‑101 (MQ=255)


                                                          ggTTTCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCgg                                        >  1:2308088/1‑101 (MQ=255)


                                                             ttCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTg                                     >  2:1554177/1‑101 (MQ=255)


                                                             ttCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGC.GGTG.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTg                                     >  2:4367638/1‑101 (MQ=255)


                                                              tCAGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTgg                                    <  1:8254213/101‑1 (MQ=255)


                                                                aGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGc                                  >  2:9121734/1‑101 (MQ=255)


                                                                aGGCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGc                                  <  1:1156675/101‑1 (MQ=255)


                                                                  gCCAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTc                                <  2:62640/101‑1 (MQ=255)


                                                                   ccAGTCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCg                               >  1:8796268/1‑101 (MQ=255)


                                                                   ccAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCg                               >  2:4866572/1‑101 (MQ=255)


                                                                    cAATCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCgg                              >  2:2582164/1‑101 (MQ=255)


                                                                      aTCAGGCTGTCT.TTGCGCCGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGcc                            <  2:7805841/101‑1 (MQ=255)


                                                                      aTCAGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGcc                            >  2:7030126/1‑101 (MQ=255)


                                                                         aGGCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAg                         <  1:723210/101‑1 (MQ=255)


                                                                          ggCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGTGCATGAGGGGCTGCGGCTGGGCTCGGCCGAgg                        >  1:4389228/1‑101 (MQ=255)


                                                                           gCGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAGGc                       >  2:4171245/1‑101 (MQ=255)


                                                                            cGGTCT.TTGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAGGCa                      >  2:1480301/1‑101 (MQ=255)


                                                                              ggtctttGCGACGCTGCCGAT.GGCGGG.G.GCTCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGGTCGGCCGAGGCAc                     >  2:6016851/6‑101 (MQ=255)


                                                                              ggtctttGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGGGGCAc                     >  2:2237016/6‑101 (MQ=255)


                                                                              ggtctttGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAGGCAc                     >  2:5091532/6‑101 (MQ=255)


                                                                              ggtctttGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAGGCAc                     >  1:1515467/6‑101 (MQ=255)


                                                                                 ctttGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAGGCACGCa                  >  1:3803916/3‑101 (MQ=255)


                                                                                  tttGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAGGCACGccg                 >  1:6743378/2‑99 (MQ=255)


                                                                                  tttGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAGGCACGCAg                 <  2:5767793/100‑1 (MQ=255)


                                                                                  tttGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAGGCACGCAg                 <  1:943254/100‑1 (MQ=255)


                                                                                  tttGCGACGCTGCCGAT.GGCGGG.G.GAGCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAGGCACGCAg                 <  2:2154757/100‑1 (MQ=255)


                                                                                    tGCGACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAGGCACGCAGCt               >  1:6478066/1‑101 (MQ=255)


                                                                                       gACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAGGCACGCAGCTTTc            >  1:7860880/1‑101 (MQ=255)


                                                                                       gACGCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAGGCACGCAGCTTTc            <  2:3287215/101‑1 (MQ=255)


                                                                                          gCTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAGGCACGCAGCTTTCGcc         >  2:8650390/1‑101 (MQ=255)


                                                                                           cTGCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAGGCACGCAGCTTTCGCca        <  1:2014154/101‑1 (MQ=255)


                                                                                             gCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAGGCACGCAGCTTTCGCcaca      <  1:5949258/101‑1 (MQ=255)


                                                                                             gCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAGGCACGCAGCTTTCGCcaca      >  2:7181520/1‑101 (MQ=255)


                                                                                             gCCGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAGGCACGCAGCTTTCGCcaca      >  2:5018785/1‑101 (MQ=255)


                                                                                              ccGAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAGGCACGCAGCTTTCGCcacca     >  2:3014532/1‑99 (MQ=255)


                                                                                                gAT.GGCGGG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAGGCACGCAGCTTTCGCCACAATg   >  2:8186157/1‑101 (MQ=255)


CTGGAGCAAGAGGCCGCCGGTTTTTTCGGTGCCGAGGCCTGTCTTTTCATGGGCGGCGGGTTTCAGGCCAATCAGGCGGTCTCTTGCGACGCTGCCGAT.GGC.GG.G.GATCTGGTGCTGCATGACGTGCTGATCCATGCCAGCGCGCATGAGGGGCTGCGGCTGGGCTCGGCCGAGGCACGCAGCTTTCGCCACAATGA  >  NC_014034/3560821‑3561017

Base quality scores:


ATCG

< 3 ≤


ATCG

< 23 ≤


ATCG

< 30 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG