Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,609,108 +G 98.5% intergenic (+47/‑124) clpB → / → dinB chaperone ClpB/DNA polymerase IV

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,609,1081.→G98.5% 12.8 ‑0.3 ‑1.7518intergenic (+47/‑124)clpB/dinBchaperone ClpB/DNA polymerase IV
Reads supporting (aligned to +/- strand):  new base (208/301):  ref base (4/4):  total (213/305)
Fisher's exact test strand distribution p-value = 7.22e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.24e-02

Only 100 of 552 total aligned reads displayed.

CAAGGTCTCGGCCAGCCAGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATACGGGAGT.GGGG.GCTCTG.CCCCCGCTTCGCTCCCCCCGGGATAT.TT.G.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGA.GGGGCAGCGCCCCCTCCCCCAC  >  NC_014034/3609010‑3609202
                                                                                                       |                                                                                                
      ctcGGCCAGCCAGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TT.g.gggca                                                                                           >  2:1684703/1‑98 (MQ=38)
      ctcGGCCAGCCAGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TT.g.gggca                                                                                           >  2:1142282/1‑98 (MQ=38)
      ctcGGCCAGCCAGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TT.g.gggca                                                                                           <  1:951433/101‑4 (MQ=38)
      ctcGGCCAGCCAGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TT.g.gggca                                                                                           <  1:4572779/101‑4 (MQ=38)
       tcGGCCAGCCAGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TT.g.gggcaa                                                                                          <  2:7279705/101‑5 (MQ=38)
        cGGCCAGCCAGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TT.GCGGCAAg                                                                                          <  1:5726743/101‑1 (MQ=37)
         ggCCAGCCAGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAgg                                                                                         >  2:7679485/1‑101 (MQ=38)
          gccagccaGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGt                                                                                        <  1:874163/101‑1 (MQ=38)
            cagccagcGCGTCCAGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTaa                                                                                      >  1:4023388/1‑101 (MQ=38)
              gccagcGCGTCCAGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTaaaa                                                                                    <  2:5999936/101‑1 (MQ=38)
                cagcGCGTCCAGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGc                                                                                  <  1:3574398/101‑1 (MQ=38)
                   cgcgTCCAGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCAtt                                                                               <  2:3604929/101‑1 (MQ=38)
                    gcgTCCAGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTg                                                                              >  2:4087531/1‑101 (MQ=37)
                    gcgTCCAGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTg                                                                              <  2:2745210/101‑1 (MQ=38)
                      gTCCAGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGcc                                                                            <  2:2187482/101‑1 (MQ=38)
                      gTCCAGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGcc                                                                            <  1:4356546/101‑1 (MQ=38)
                      gTCCAGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGcc                                                                            >  1:1017086/1‑101 (MQ=37)
                       tCCAGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGccc                                                                           >  2:8809246/1‑101 (MQ=37)
                       tCCAGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGccc                                                                           <  2:8017179/101‑1 (MQ=38)
                        ccAGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCt                                                                          <  1:7966727/101‑1 (MQ=38)
                         cAGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCtt                                                                         >  1:1206858/1‑101 (MQ=37)
                         cAGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCtt                                                                         >  1:7751931/1‑101 (MQ=37)
                          aGACCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCttt                                                                        <  2:5598678/101‑1 (MQ=38)
                            gccGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTAt                                                                      <  2:9054747/100‑1 (MQ=38)
                            aCCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTAt                                                                      <  2:5364497/101‑1 (MQ=38)
                            aCCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTAt                                                                      <  2:488522/101‑1 (MQ=38)
                            aCCGAACCGCGCGTGCTGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTAt                                                                      <  1:1377593/101‑1 (MQ=38)
                               gAACCGCGCGTGCTGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCtt                                                                   <  2:7526699/101‑1 (MQ=38)
                               gAACCGCGCGTGCTGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCtt                                                                   <  2:5703479/101‑1 (MQ=38)
                               gAACCGCGCGTGCTGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCtt                                                                   <  1:1519617/101‑1 (MQ=38)
                               gAACCGCGCGTGCTGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCtt                                                                   <  1:1959250/101‑1 (MQ=38)
                               gAACCGCGCGTGCTGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCtt                                                                   >  1:335875/1‑101 (MQ=37)
                                aaCCGCGCGTGCTGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTg                                                                  <  1:6573447/101‑1 (MQ=38)
                                aaCCGCGCGTGCTGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTg                                                                  <  1:2794238/101‑1 (MQ=38)
                                aaCCGCGCGTGCTGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTg                                                                  >  1:7325499/1‑101 (MQ=37)
                                aaCCGCGCGTGCTGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTg                                                                  <  1:5251398/101‑1 (MQ=37)
                                aaCCGCGCGTGCTGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCCCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTg                                                                  <  2:4969734/101‑1 (MQ=37)
                                 aCCGCGCGTGCTGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGc                                                                 <  1:1772503/101‑1 (MQ=38)
                                     cgcgTGCTGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCa                                                             <  1:1445865/101‑1 (MQ=38)
                                       cgTGCTGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGa                                                           <  2:2419371/101‑1 (MQ=38)
                                       cgTGCTGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGa                                                           >  1:5163219/1‑101 (MQ=37)
                                        gTGCTGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGat                                                          <  1:8888813/101‑1 (MQ=38)
                                          gCTGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGatat                                                        <  1:3113075/101‑1 (MQ=38)
                                           cTGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATc                                                       <  1:4839146/101‑1 (MQ=38)
                                           cTGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATc                                                       <  2:6592830/101‑1 (MQ=38)
                                            tGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATcc                                                      <  2:3309426/101‑1 (MQ=37)
                                            tGAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATcc                                                      >  1:227831/1‑101 (MQ=37)
                                             gAACTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATccc                                                     <  2:1807509/101‑1 (MQ=37)
                                             gAACTGATACGGGAGT.GGGGCGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATACccc                                                     <  1:7218910/101‑1 (MQ=37)
                                               aCTGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCgg                                                   <  1:6414695/101‑1 (MQ=37)
                                                 tGATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGAATTGCCCTTTATCTTGCCTCAGATATCCCgggg                                                 >  2:5483361/1‑101 (MQ=37)
                                                  gATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCggggg                                                >  1:4635604/1‑101 (MQ=37)
                                                  gATACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCggggg                                                >  1:8095953/1‑101 (MQ=37)
                                                   aTACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGt                                               <  2:7225960/101‑1 (MQ=38)
                                                    tACGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTg                                              >  2:5241882/1‑101 (MQ=37)
                                                     aCGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGa                                             <  2:8498151/101‑1 (MQ=37)
                                                      cGGGAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGaa                                            <  2:2025906/101‑1 (MQ=37)
                                                        ggAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAAtt                                          <  2:314113/101‑1 (MQ=37)
                                                        ggAGTGGGGG.GCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAAtt                                          >  2:815096/1‑101 (MQ=25)
                                                         gagtggggg.gCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAAttt                                         <  2:5900328/96‑1 (MQ=37)
                                                         gagtggggg.gCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAAttt                                         <  2:3703104/96‑1 (MQ=37)
                                                          agtggggg.gCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAAtttt                                        >  2:2543160/5‑101 (MQ=37)
                                                               ggg.gCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGgtgc                                   <  2:3461469/101‑1 (MQ=37)
                                                                 g.gCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAg                                 >  2:132826/1‑101 (MQ=37)
                                                                    ctctGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGcc                               >  1:3692664/1‑101 (MQ=37)
                                                                    ctctGCCCCCCGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGcc                               >  2:5932330/1‑101 (MQ=37)
                                                                     tctgccccccGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCa                              >  1:8559762/6‑101 (MQ=255)
                                                                     tctgccccccGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCa                              <  1:2404036/96‑1 (MQ=255)
                                                                     tctgccccccGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCa                              <  1:7529353/96‑1 (MQ=255)
                                                                      ctgccccccGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCaa                             >  1:2727405/5‑101 (MQ=255)
                                                                      ctgccccccGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCaa                             >  2:3909513/5‑101 (MQ=255)
                                                                         ccccccGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAag                          <  1:713897/100‑1 (MQ=255)
                                                                         ccccccGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAag                          >  2:4398453/2‑101 (MQ=255)
                                                                         ccccccGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAag                          <  2:2909750/100‑1 (MQ=255)
                                                                           ccccGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAaga.g                       <  1:4231850/101‑1 (MQ=255)
                                                                           ccccGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAaga.g                       >  1:1621713/1‑101 (MQ=255)
                                                                           ccccGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGGGAATTTTGGGGCAGCCAAAaga.g                       >  2:4233507/1‑101 (MQ=38)
                                                                            cccGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGA.gg                      <  2:12568/101‑1 (MQ=255)
                                                                            cccGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCGCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGA.gg                      <  2:1530539/101‑1 (MQ=255)
                                                                             ccGCTTCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGA.ggg                     <  1:1296997/101‑1 (MQ=255)
                                                                                cTTCGCTCCCCCCGGGATATCTG.G.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGA.ggcggc                  <  1:8817845/101‑5 (MQ=25)
                                                                                 ttCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGA.gggggca                 <  1:5923237/101‑4 (MQ=255)
                                                                                  tCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGA.gggggcag                <  2:6314741/101‑5 (MQ=255)
                                                                                  tCGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGA.gggggcag                <  2:4799232/101‑5 (MQ=255)
                                                                                   cGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGA.gggggcagc               >  1:7403643/1‑96 (MQ=255)
                                                                                   cGCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGA.gggggcagc               <  2:2806388/101‑6 (MQ=255)
                                                                                    gCTCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcg               >  1:3894391/1‑101 (MQ=25)
                                                                                      tCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcc             >  1:6146246/1‑101 (MQ=21)
                                                                                      tCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcc             >  1:2137916/1‑101 (MQ=21)
                                                                                      tCCCCCCGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATACCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcc             >  1:827783/1‑101 (MQ=21)
                                                                                       ccccccGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccc            <  1:9006366/101‑1 (MQ=21)
                                                                                           ccGGGATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTc        <  2:7372223/101‑1 (MQ=21)
                                                                                             gggATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTccc      <  2:8666939/101‑1 (MQ=21)
                                                                                              ggATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTcccc     >  1:8364358/1‑101 (MQ=21)
                                                                                              ggATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTcccc     <  2:1491972/101‑1 (MQ=18)
                                                                                               gATAT.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTccccc    >  1:6921490/1‑101 (MQ=21)
                                                                                                atat.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCa   >  2:6821395/1‑101 (MQ=21)
                                                                                                 tat.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTccccctc  <  1:5483334/101‑3 (MQ=18)
                                                                                                 tat.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCAc  >  1:6834688/1‑101 (MQ=21)
                                                                                                 tat.TTGG.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTCCCCCAc  <  2:7096981/101‑1 (MQ=18)
                                                                                                       |                                                                                                
CAAGGTCTCGGCCAGCCAGCGCGTCCAGACCGAACCGCGCGTGCTGAACTGATACGGGAGT.GGGG.GCTCTG.CCCCCGCTTCGCTCCCCCCGGGATAT.TT.G.GGCAAGGTAAAAGCATTGCCCTTTATCTTGCCTCAGATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGA.GGGGCAGCGCCCCCTCCCCCAC  >  NC_014034/3609010‑3609202

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG