BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,648,333	+G	99.3%	intergenic (‑83/‑73)	serB ← / → serC	phosphoserine phosphatase SerB/phosphoserine aminotransferase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,648,333	1	.→G	99.3%	2.2 ‑1.0 ‑1.5	534	intergenic (‑83/‑73)	serB/serC	phosphoserine phosphatase SerB/phosphoserine aminotransferase
Reads supporting (aligned to +/- strand): new base (262/268): ref base (3/1): total (265/269)
Fisher's exact test strand distribution p-value = 3.70e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.17e-02

Only 100 of 591 total aligned reads displayed.


AAGCGTGGCGATGAACATGGATCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.A.G.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATGACGAATACTGCCCCGGCCAA  >  NC_014034/3648233‑3648428


 aGCGTGGCGATGAACATGGATCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCag.a.g                                                                                                   <  2:1152152/101‑1 (MQ=255)


   cGTGGCGATGAACATGGATCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.A.g.g.g                                                                                               >  1:3883042/1‑101 (MQ=255)


    gTGGCGATGAACATGGATCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.A.g.g.gg                                                                                              >  2:5362485/1‑100 (MQ=255)


    gTGGCGATGAACATGGATCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.A.g.g.gg                                                                                              >  2:3264601/1‑100 (MQ=255)


     tGGCGATGAACATGGATCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.A.g.g.ggc                                                                                             >  1:1323835/1‑99 (MQ=255)


      ggCGATGAACATGGATCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.A.g.g.ggca                                                                                            <  1:8928230/101‑4 (MQ=255)


      ggCGATGAACATGGATCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.A.g.g.ggca                                                                                            <  1:557215/101‑4 (MQ=255)


        cGATGAACATGGATCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.A.g.g.ggcaaa                                                                                          >  1:191134/1‑96 (MQ=255)


        cGATGAACATGGATCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.A.g.g.ggcaaa                                                                                          <  1:6424306/101‑6 (MQ=255)


           tGAACATGGATCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.A.G.G.G.Gaaaagcc                                                                                      >  2:2917037/1‑97 (MQ=255)


            gAACATGGATCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAGCG.G.G.G.CAAAGCCt                                                                                      >  2:8331963/1‑101 (MQ=255)


            gAACATGGATCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCt                                                                                      >  2:6323863/1‑101 (MQ=255)


            gAACATGGATCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCt                                                                                      >  2:3623056/1‑101 (MQ=255)


             aaCATGGATCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTg                                                                                     >  1:7107736/1‑101 (MQ=255)


              aCATGGATCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGt                                                                                    >  2:4142716/1‑101 (MQ=255)


              aCATGGATCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCAACTTGTGCCAG.AGG.G.G.CAAAGCCTGt                                                                                    >  1:5240038/1‑101 (MQ=255)


                 tGGATCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTagc                                                                                 <  2:7217429/101‑1 (MQ=255)


                  ggATCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTagca                                                                                <  2:133926/101‑1 (MQ=255)


                  ggATCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTagca                                                                                <  2:5506488/101‑1 (MQ=255)


                  ggATCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTagca                                                                                >  1:2543487/1‑101 (MQ=255)


                  ggATCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTagca                                                                                <  1:8467647/101‑1 (MQ=255)


                    aTCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTagcagc                                                                              >  2:5924445/1‑101 (MQ=255)


                    aTCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTagcagc                                                                              <  2:7680264/101‑1 (MQ=255)


                    aTCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTagcagc                                                                              <  1:5793004/101‑1 (MQ=255)


                     tCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCg                                                                             <  2:4049090/101‑1 (MQ=255)


                      cAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGa                                                                            <  1:1775346/101‑1 (MQ=255)


                       aGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGAc                                                                           <  2:816368/101‑1 (MQ=255)


                        ggggTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGAcg                                                                          <  2:6145904/101‑1 (MQ=255)


                        ggggTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGAcg                                                                          >  1:4686455/1‑101 (MQ=255)


                        ggggTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGAcg                                                                          <  1:8045670/101‑1 (MQ=255)


                             cccGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCggg                                                                     >  1:221193/1‑101 (MQ=255)


                             cccGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCggg                                                                     <  2:8893972/101‑1 (MQ=255)


                               cGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGac                                                                   >  2:4269261/1‑101 (MQ=255)


                               cGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGac                                                                   <  1:886660/101‑1 (MQ=255)


                                ggggCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.A.G.G.GTCAAAGCCTGTAGCAGCGACGGCGGGaca                                                                  >  2:2780678/1‑101 (MQ=255)


                                 gggCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGacac                                                                 >  2:1991040/1‑101 (MQ=255)


                                 gggCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGacac                                                                 >  1:1480074/1‑101 (MQ=255)


                                  ggCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACAcc                                                                <  1:6050663/101‑1 (MQ=255)


                                          ggATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCa                                                        <  2:492713/101‑1 (MQ=255)


                                          ggATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCa                                                        >  1:782567/1‑101 (MQ=255)


                                            aTGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAAc                                                      >  2:6995652/1‑101 (MQ=255)


                                            aTGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAAc                                                      <  1:1056519/101‑1 (MQ=255)


                                            aTGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAAc                                                      <  1:3718382/101‑1 (MQ=255)


                                            aTGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAAc                                                      <  1:2670959/101‑1 (MQ=255)


                                            aTGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAAc                                                      <  1:3102690/101‑1 (MQ=255)


                                            aTGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAAc                                                      <  1:6137598/101‑1 (MQ=255)


                                              gTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATTTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGa                                                    >  1:5005385/1‑101 (MQ=255)


                                              gTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGa                                                    >  1:4418182/1‑101 (MQ=255)


                                                cGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAg                                                  >  2:8538856/1‑101 (MQ=255)


                                                 gCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAgg                                                 >  1:6503647/1‑101 (MQ=255)


                                                  cTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAggg                                                >  2:6043791/1‑101 (MQ=255)


                                                   ttttGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGc                                               >  1:7740330/1‑101 (MQ=255)


                                                    tttGATATCCGATGCGGCGCTTTAGCGTGATTTGCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCg                                              >  1:8207407/1‑101 (MQ=255)


                                                      tGATATCCGATGCGGCGCTTTAGGGGGATTTTCCATTTTGGCCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGCCGGCGGGACACCCTTCCCCACCGAAGGGGGtt                                            >  2:2455509/1‑101 (MQ=255)


                                                      tGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGtt                                            >  2:4712461/1‑101 (MQ=255)


                                                       gATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGttt                                           >  1:5692100/1‑101 (MQ=255)


                                                        atatCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTa                                          >  2:2250502/1‑101 (MQ=255)


                                                         tttcggATGCGGCGCTTTAGCTTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTAt                                         <  1:2939109/96‑1 (MQ=255)


                                                          atCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAGGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATc                                        >  1:5415035/1‑101 (MQ=255)


                                                          atCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATc                                        >  2:5207054/1‑101 (MQ=255)


                                                           tCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.A.G.GCG.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCt                                       <  1:8285446/101‑1 (MQ=255)


                                                            ccGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTg                                      <  2:1531255/101‑1 (MQ=255)


                                                            ccGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTg                                      <  2:3150335/101‑1 (MQ=255)


                                                            ccGAGGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTg                                      <  2:3734424/101‑1 (MQ=255)


                                                              gATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGa                                    >  1:4583218/1‑101 (MQ=255)


                                                               aTGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACCCCCTTCCCCAACGAAGGGCGTTTATCTGGaa                                   <  1:5965858/101‑1 (MQ=255)


                                                                 gcggcgCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAgg                                 >  1:6727567/1‑101 (MQ=255)


                                                                  cggcgCTTTAGCGTGATTTTCCATCTTGTGCCAG.A.GTG.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGa                                >  2:6873695/1‑101 (MQ=255)


                                                                   ggcgCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGAt                               >  1:2867175/1‑101 (MQ=255)


                                                                   ggcgCTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGAt                               <  1:3191134/101‑1 (MQ=255)


                                                                    gcgcTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATa                              <  1:936904/101‑1 (MQ=255)


                                                                      gcTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGc                            >  1:1685209/1‑101 (MQ=255)


                                                                       cTTTAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCg                           <  1:8100858/101‑1 (MQ=255)


                                                                          tAGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACa                        >  1:2046807/1‑101 (MQ=255)


                                                                           aGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACAt                       <  1:3585241/101‑1 (MQ=255)


                                                                           aGCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACAt                       <  2:5051944/101‑1 (MQ=255)


                                                                            gCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATg                      >  2:7545973/1‑101 (MQ=255)


                                                                            gCGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATg                      <  1:5091297/101‑1 (MQ=255)


                                                                             cGTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATGa                     <  1:4299189/101‑1 (MQ=255)


                                                                              gTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATGAc                    <  2:8686006/101‑1 (MQ=255)


                                                                              gTGATTTTCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCAACATGAc                    >  2:6246540/1‑101 (MQ=255)


                                                                                  ttttCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATGACGAAt                <  1:6324103/101‑1 (MQ=255)


                                                                                   tttCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATGACGAATa               <  2:1875061/101‑1 (MQ=255)


                                                                                   tttCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATGACGAATa               <  2:6713801/101‑1 (MQ=255)


                                                                                     tCCATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATGACGAATACt             <  2:7841965/101‑1 (MQ=255)


                                                                                      ccATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATGACGAATACTg            >  1:4058254/1‑101 (MQ=255)


                                                                                      ccATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATGACGAATACTg            <  2:3463568/101‑1 (MQ=255)


                                                                                       cATCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATGACGAATACTGc           <  1:7976696/101‑1 (MQ=255)


                                                                                        aTCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATGACGAATACTGcc          >  1:3372529/1‑101 (MQ=255)


                                                                                        aTCTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATGACGAATACTGcc          >  2:3932723/1‑101 (MQ=255)


                                                                                          cTTGTGCCAG.AGG.G.G.CANAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATGACGAATACTGcccc        <  2:1672289/101‑1 (MQ=255)


                                                                                          cTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATGACGAATACTGcccc        <  2:65545/101‑1 (MQ=255)


                                                                                          cTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATGACGAATACTGcccc        <  2:5702647/101‑1 (MQ=255)


                                                                                          cTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATGACGAATACTGcccc        >  1:9060272/1‑101 (MQ=255)


                                                                                          cTTGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATGACGAATACTGcccc        <  1:6942056/101‑1 (MQ=255)


                                                                                           ttGTGCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATGACGAATACTGCCCCg       <  2:9056956/101‑1 (MQ=255)


                                                                                             gtgCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATGACGAATACTGCCCCGGc     >  1:2706834/1‑101 (MQ=255)


                                                                                             gtgCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATGACGAATACTGCCCCGGc     >  1:8575827/1‑101 (MQ=255)


                                                                                               gCCAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATGACGAATACTGCCCCGGCCa   >  2:2563583/1‑101 (MQ=255)


                                                                                                ccAG.AGG.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATGACGAATACTGCCCCGGCCaa  <  1:7472674/101‑1 (MQ=255)


AAGCGTGGCGATGAACATGGATCAGGGGTCCCGGGGCTGGAGGGATGTCGCTTTTGATATCCGATGCGGCGCTTTAGCGTGATTTTCCATCTTGTGCCAG.A.G.G.G.CAAAGCCTGTAGCAGCGACGGCGGGACACCCTTCCCCAACGAAGGGCGTTTATCTGGAAGGATAGCGACATGACGAATACTGCCCCGGCCAA  >  NC_014034/3648233‑3648428

Base quality scores:


ATCG

< 3 ≤


ATCG

< 24 ≤


ATCG

< 31 ≤


ATCG

< 35 ≤


ATCG

< 41 ≤


ATCG