Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 408,497 +GCAGA 100% intergenic (+115/+33) glnA1 → / ← dapA glutamine synthetase/dihydrodipicolinate synthase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034408,4971.→G100.0% 190.4 325intergenic (+115/+33)glnA1/dapAglutamine synthetase/dihydrodipicolinate synthase
Reads supporting (aligned to +/- strand):  new base (161/105):  ref base (22/35):  total (183/142)
Fisher's exact test strand distribution p-value = 3.09e-03
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.22e-01
*NC_014034408,4972.→C100.0% 194.5 325intergenic (+115/+33)glnA1/dapAglutamine synthetase/dihydrodipicolinate synthase
Reads supporting (aligned to +/- strand):  new base (161/105):  ref base (22/36):  total (183/142)
Fisher's exact test strand distribution p-value = 2.06e-03
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.32e-01
*NC_014034408,4973.→A100.0% 189.5 325intergenic (+115/+33)glnA1/dapAglutamine synthetase/dihydrodipicolinate synthase
Reads supporting (aligned to +/- strand):  new base (161/104):  ref base (22/36):  total (183/142)
Fisher's exact test strand distribution p-value = 1.99e-03
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.88e-01
*NC_014034408,4974.→G100.0% 193.5 325intergenic (+115/+33)glnA1/dapAglutamine synthetase/dihydrodipicolinate synthase
Reads supporting (aligned to +/- strand):  new base (161/105):  ref base (22/36):  total (183/142)
Fisher's exact test strand distribution p-value = 2.06e-03
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.36e-01
*NC_014034408,4975.→A100.0% 193.0 325intergenic (+115/+33)glnA1/dapAglutamine synthetase/dihydrodipicolinate synthase
Reads supporting (aligned to +/- strand):  new base (161/105):  ref base (22/37):  total (183/142)
Fisher's exact test strand distribution p-value = 1.37e-03
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.95e-01

Only 100 of 339 total aligned reads displayed.

CTGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGCAAAGACGGGGG.....GCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGCGCGGC  >  NC_014034/408396‑408571
                                                                                                      |||||                                                                          
ctGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACggggg                                                                                 >  2:8799249/1‑101 (MQ=255)
 tGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACggggg.....g                                                                           >  2:9116496/1‑101 (MQ=255)
 tGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACggggg.....g                                                                           <  2:5073073/101‑1 (MQ=255)
  gCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGG.....Gc                                                                          <  2:6374518/101‑1 (MQ=255)
  gCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGG.....Gc                                                                          >  1:3567729/1‑101 (MQ=255)
  gCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGG.....Gc                                                                          >  2:151177/1‑101 (MQ=255)
  gCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGG.....Gc                                                                          >  2:8540878/1‑101 (MQ=255)
   cccccAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGG.....Gca                                                                         >  1:7928754/1‑100 (MQ=255)
    ccccAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGG.....Gcag                                                                        >  1:2259120/1‑99 (MQ=255)
    ccccAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGG.....Gcag                                                                        >  1:4538286/1‑99 (MQ=255)
    ccccAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGG.....Gcag                                                                        >  1:4148870/1‑99 (MQ=255)
     cccAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGG.....Gcaga                                                                       <  2:8124148/101‑4 (MQ=255)
     cccAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGG.....Gcaga                                                                       >  2:622030/1‑98 (MQ=255)
      ccAGGCCGCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGG.....Gcagag                                                                      <  2:1743271/101‑5 (MQ=25)
      ccAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGG.....Gcagag                                                                      <  1:8326328/101‑5 (MQ=255)
        aGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGG.....Gcagagcc                                                                    <  2:6676103/101‑7 (MQ=255)
         gACCCCCGCGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGAAGGCTTGCTGAAAGC.AAGACGGGGG.....Gcagagccc                                                                   <  2:1263426/101‑8 (MQ=25)
          aCCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGG.....Gcagagcccc                                                                  <  1:640535/101‑9 (MQ=25)
           cccccGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGG.....Gcagagccccc                                                                 <  1:3009157/101‑10 (MQ=25)
            cccgGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGG.....Gcagcgcccccc                                                                <  2:4879064/101‑11 (MQ=25)
            ccccGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGG.....Gcagagcccccc                                                                <  1:7631890/101‑11 (MQ=25)
            ccccGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGG.....Gcagagcccccc                                                                <  2:1463592/101‑11 (MQ=25)
            ccccGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCGTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGG.....Gcagagcccccc                                                                >  2:4430638/1‑91 (MQ=25)
                        tGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGcc                                                         >  1:5267958/1‑101 (MQ=21)
                         gCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCa                                                        <  1:1388380/101‑1 (MQ=21)
                          cGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCaa                                                       >  2:1039062/1‑101 (MQ=21)
                           ggCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCaaa                                                      >  2:6507325/1‑101 (MQ=21)
                           ggCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCaaa                                                      <  2:2692444/101‑1 (MQ=21)
                            gCAAGAGGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCaaaa                                                     <  1:4808972/101‑1 (MQ=18)
                             cAAGGTGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAg                                                    <  1:8940895/101‑1 (MQ=18)
                              aaGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAgg                                                   <  1:2419506/101‑1 (MQ=21)
                               aGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGga                                                  >  2:361724/1‑101 (MQ=21)
                                gATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGgac                                                 >  2:5952498/1‑101 (MQ=21)
                                 aTGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGgacc                                                <  2:9022635/101‑1 (MQ=21)
                                 aTGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGgacc                                                <  1:6892416/101‑1 (MQ=21)
                                 aTGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGgacc                                                <  2:2576787/101‑1 (MQ=21)
                                 aTGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGgacc                                                >  2:2480633/1‑101 (MQ=21)
                                  tGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGgaccg                                               <  1:8675696/101‑1 (MQ=21)
                                  tGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGgaccg                                               <  2:2262755/101‑1 (MQ=21)
                                  tGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGgaccg                                               >  2:3730788/1‑101 (MQ=21)
                                  tGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGgaccg                                               >  2:7388792/1‑101 (MQ=21)
                                   gAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGgaccga                                              >  1:5650191/1‑101 (MQ=21)
                                   gAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGgaccga                                              >  1:8468804/1‑101 (MQ=21)
                                   gAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGgaccga                                              >  2:5467197/1‑101 (MQ=21)
                                      gCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCt                                           <  1:1366268/101‑1 (MQ=21)
                                      gCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCt                                           <  1:2603774/101‑1 (MQ=21)
                                       ccGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTc                                          <  2:3346537/101‑1 (MQ=21)
                                        cGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCa                                         >  2:4259936/1‑101 (MQ=21)
                                        cGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCa                                         <  2:1972760/101‑1 (MQ=21)
                                        cGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCa                                         <  2:6791777/101‑1 (MQ=21)
                                         gATGTCCTTCATCTTGCCTCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCTCACAGGTCGCCAAAAGGACCGACCTCAg                                        <  1:7529493/101‑1 (MQ=18)
                                         gATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAg                                        >  1:1176871/1‑101 (MQ=21)
                                         gATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAg                                        >  2:6120351/1‑101 (MQ=21)
                                          aTGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGa                                       >  1:4345915/1‑101 (MQ=21)
                                            gTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATc                                     >  1:913219/1‑101 (MQ=21)
                                            gTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATc                                     >  1:2115516/1‑101 (MQ=21)
                                            gTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATc                                     >  2:4596729/1‑101 (MQ=21)
                                             ccttccaccTTGCCGCAAATAGCCGGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGCCGCCAAAAGGACCGACCTCAGATCa                                    <  2:6950147/93‑1 (MQ=255)
                                              ccTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAg                                   >  1:1024593/1‑101 (MQ=21)
                                              ccTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAg                                   >  1:1048972/1‑101 (MQ=21)
                                                ttCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGcc                                 >  2:7696724/1‑101 (MQ=21)
                                                ttCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGcc                                 <  1:5836434/101‑1 (MQ=21)
                                                 tCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGccc                                <  2:5405459/101‑1 (MQ=21)
                                                 tCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGccc                                >  1:6747399/1‑101 (MQ=21)
                                                 tCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGccc                                >  1:618132/1‑101 (MQ=21)
                                                  cATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCg                               <  2:7508420/101‑1 (MQ=21)
                                                   aTCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCgg                              >  1:9062095/1‑101 (MQ=21)
                                                   aTCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCgg                              >  2:351022/1‑101 (MQ=21)
                                                   aTCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCgg                              <  1:7049392/101‑1 (MQ=21)
                                                    tCTGGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGAGCGACCTCAGATCAGCCCGGc                             >  1:7219040/1‑101 (MQ=21)
                                                     cTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCg                            <  1:199099/101‑1 (MQ=25)
                                                     cTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCg                            >  1:4013261/1‑101 (MQ=25)
                                                     cTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCg                            >  1:3636901/1‑101 (MQ=25)
                                                       tGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCgtg                          >  2:2111254/1‑101 (MQ=25)
                                                        gCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCgtgt                         >  1:309959/1‑101 (MQ=25)
                                                         ccGCAAATCTCCCGGGGGGATGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGtt                        <  1:4291086/101‑1 (MQ=255)
                                                          cGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTg                       <  2:6288574/101‑1 (MQ=25)
                                                            cAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCa                     >  1:3429418/1‑101 (MQ=25)
                                                             aaaTATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCAt                    <  2:5561056/101‑1 (MQ=255)
                                                              aaTATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATc                   >  2:3039564/1‑101 (MQ=255)
                                                              aaTATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATc                   >  2:4690766/1‑101 (MQ=255)
                                                              aaTATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATc                   >  2:7130289/1‑101 (MQ=255)
                                                              aaTATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCC.AAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATcg                  <  2:7555893/101‑1 (MQ=255)
                                                                tatCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATcgc                 <  1:2866661/101‑1 (MQ=255)
                                                                 atCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATcgcg                >  2:2911353/1‑101 (MQ=255)
                                                                 atCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATcgcg                >  2:321969/1‑101 (MQ=255)
                                                                 atCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATcgcg                >  1:8055119/1‑101 (MQ=255)
                                                                 atCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATcgcg                >  2:1832102/1‑101 (MQ=255)
                                                                    ccGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCgcggcg             >  1:1592587/1‑101 (MQ=255)
                                                                    ccGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCgcggcg             >  1:506106/1‑101 (MQ=255)
                                                                      ggggggAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTc           >  1:3224625/1‑101 (MQ=255)
                                                                        ggggAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCaa         <  1:7032889/101‑1 (MQ=255)
                                                                        ggggAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCaa         <  1:3919821/101‑1 (MQ=255)
                                                                          ggAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAgc       <  2:4037465/101‑1 (MQ=255)
                                                                           gAGGCTTTCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAgcg      >  1:6009685/1‑101 (MQ=255)
                                                                           gAGGCTTGCTGACAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAgcg      >  1:8190403/1‑101 (MQ=255)
                                                                           gAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAgcg      >  2:5216786/1‑101 (MQ=255)
                                                                           gAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAgcg      <  1:5364533/101‑1 (MQ=255)
                                                                               cTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGCGCGGc  >  1:5209011/1‑101 (MQ=255)
                                                                               cTTGCTGAAAGC.AAGACGGGGGGCAGAGCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGCGCGGc  >  2:919753/1‑101 (MQ=255)
                                                                                                      |||||                                                                          
CTGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGCAAAGACGGGGG.....GCCCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGCGCGGC  >  NC_014034/408396‑408571

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG