Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 568,801 +C 1.6% intergenic (‑97/+69) RCAP_rcc00530 ← / ← carB hypothetical protein/carbamoyl‑phosphate synthase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034568,8011.→C 1.6% 6.8 ‑0.4 ‑0.5304intergenic (‑97/+69)RCAP_rcc00530/carBhypothetical protein/carbamoyl‑phosphate synthase large subunit
Reads supporting (aligned to +/- strand):  new base (4/1):  ref base (188/111):  total (192/112)
Fisher's exact test strand distribution p-value = 6.55e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.26e-01

Only 100 of 409 total aligned reads displayed.

TCATTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAA.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGATTTCGC  >  NC_014034/568701‑568901
                                                                                                       |                                                                                                     
 cATTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAA.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.c                                                                                                      >  1:264970/1‑101 (MQ=255)
  aTTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAA.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.cc                                                                                                     <  1:4725259/101‑1 (MQ=255)
     ccTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAA.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.ccccc                                                                                                  <  2:2572768/101‑1 (MQ=255)
     ccTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAA.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.ccccc                                                                                                  >  1:4024666/1‑101 (MQ=255)
      cTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAA.TGACCCGCACGGGCTTGTGAA.GGCGCGGCGGCATCCTG.cccccc                                                                                                 >  2:2374818/1‑101 (MQ=255)
       tGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAA.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.ccccccc                                                                                                >  1:7332648/1‑101 (MQ=255)
          cTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAA.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.ccccccccct                                                                                             <  2:5944174/101‑2 (MQ=255)
          cTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAA.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.ccccccccc.c                                                                                            <  1:8515409/101‑1 (MQ=255)
          cTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAA.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.ccccccccc.c                                                                                            >  1:5922877/1‑101 (MQ=255)
              ggTTTCGTTATGTAAACGGGGGTGTGGGCCTGGGGCTGGGGGGGGTCAA.GGCCCCGCGCGGG.TTGGGAAGGGCGTTGCGGCTTCCTG.CCCCCCCCC.CGACg                                                                                        <  2:2295552/100‑1 (MQ=255)
              aGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAA.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGGCg                                                                                        >  1:8752847/1‑101 (MQ=255)
              aGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAA.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACg                                                                                        >  2:1159072/1‑101 (MQ=255)
                  tCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAA.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACggggg                                                                                    >  1:1102198/1‑99 (MQ=255)
                   cTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAA.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGCCGGGggg                                                                                   >  1:8954780/1‑101 (MQ=255)
                   cTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAA.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGagg                                                                                   <  1:3520419/101‑1 (MQ=255)
                   cTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAA.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CCCCGGGggg                                                                                   >  1:2783856/1‑101 (MQ=255)
                   cTGTATGTGAACCGGGGCTTACGCCTGTGGCTCCGGTGGGGCAA.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGagg                                                                                   <  2:6769172/101‑1 (MQ=255)
                    tGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAA.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG..CCCCCCCC.CGACGGGggggc                                                                                 >  1:7640163/1‑101 (MQ=255)
                     gTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAA.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CCACGGGagggc                                                                                 >  1:633907/1‑101 (MQ=255)
                      tATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAA.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CCACGGGggggca                                                                                >  1:2418803/1‑101 (MQ=255)
                       aTGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAA.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGagggcgg                                                                               >  2:1923129/1‑99 (MQ=255)
                        tgtgAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAA.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGagggcagg                                                                              <  1:1887081/101‑1 (MQ=255)
                        tgtgAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAA.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGagggcagg                                                                              <  1:7854023/101‑1 (MQ=255)
                        tgtgAACCGGGGCTTACGCCTGGGGCTGCGGTGGGGCAA.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGagggcagg                                                                              <  2:2713571/101‑1 (MQ=255)
                        tgggAACCGGGGCTTACGCCGGTGGCTGCGGGGGGGCAA.TGCCCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGagggcagg                                                                              <  2:4845641/98‑1 (MQ=255)
                         gtgAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAA.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.ACCCCCCCC.CGACGGGagggcaggg                                                                             >  1:5546251/1‑101 (MQ=255)
                         gggAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAA.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGagggcaggg                                                                             <  1:4467919/99‑1 (MQ=255)
                            aaCCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAA.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CCACGGGGGGGCAGGGCTg                                                                          >  1:6908051/1‑101 (MQ=255)
                               cGGGCCTTACGCCTGTGGGTGCGGTGGGGCAA.TGACCCGCAGGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGtt                                                                       <  2:7473658/101‑1 (MQ=255)
                                ggggCTTACGCCTGTGGCTGCGGTGGGGCAA.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGgtt                                                                      >  2:6639782/1‑101 (MQ=255)
                                 gggCTTACGCCTGTGGCTGCGGTGGGGCAA.TGGCCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTc                                                                     <  2:5825885/101‑1 (MQ=255)
                                   gCTTACGCCTGTGGCTGCGGTGGGGCAA.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG..CCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCggg                                                                  <  2:1770887/101‑1 (MQ=255)
                                     ttACGCCTGTGGCTGCGGTGGGGCAA.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCCGGTTTCgggg                                                                 >  2:4906780/1‑100 (MQ=255)
                                       aCGCCTGTGGCTGCGGTGGGGCAA.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGggcgg                                                               <  1:7376714/101‑1 (MQ=255)
                                       aCGCCTGTGGCTGCGGTGGGGCAA.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGGTTCggcggg                                                               >  2:3776359/1‑97 (MQ=255)
                                        cGCCTGTGGCTGCGGTGGGGCAA.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGGGGGCAGGGCTGGTTTCGgggggg                                                              >  1:3026958/1‑100 (MQ=255)
                                         gCCTGTGGCTGCGGTGGGGCAA.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGGTTCGGGCGGCa                                                             >  2:6168460/1‑101 (MQ=255)
                                         gCCTGGGGCTGGGGGGGGGCAA.TGGCCCGCCCGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCa                                                             <  2:5121189/101‑1 (MQ=255)
                                          gcTGTGGCTGCGGTGGGGCAA.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG..CCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAg                                                           <  1:5100592/100‑1 (MQ=255)
                                                gccgggggggggCAATTGCCCCGC.CGGGCTTGTGAA.GGCGCTTCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGcc                                                      <  1:210054/93‑1 (MQ=255)
                                                gCTGCGGTGGGGCAA.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGCCGGGGGGGCAGGGCTGGTTTCGGGCGGAAAGGCGcc                                                      >  2:3898110/1‑101 (MQ=255)
                                                gCTGCGGTGGGGCAA.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGcc                                                      >  1:5363030/1‑101 (MQ=255)
                                                 gtggggTGGGGCAA.GGCCCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCGG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGccc                                                     <  1:5214871/97‑1 (MQ=255)
                                                 cgggggTGGGGCAA.TGCCCCGCCGGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGccc                                                     <  2:1552884/97‑1 (MQ=255)
                                                 cTGCGGTGGGGCAA.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGccc                                                     <  1:9184755/101‑1 (MQ=255)
                                                 cTGCGGTGGGGCAA.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGccc                                                     <  1:6099965/101‑1 (MQ=255)
                                                    cGGTGGGGCAA.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGGTTCGGGCGGCAAGGCGCCcgcg                                                  >  2:6973544/1‑101 (MQ=255)
                                                     ggTGGGGCAA.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCCACGACGGGAGGGCAGGGCTGGTTTCGGGCGGCACGGCGCCcgcg                                                  >  2:8157480/1‑101 (MQ=255)
                                                     ggTGGGGCAA.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCcgcgg                                                 >  1:2504382/1‑101 (MQ=255)
                                                      ggtgggCAA.TGACCCGCTCGGGCTTTTGAA.GGCGCTGCGGCAGCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCcgcggc                                                <  1:3967565/98‑1 (MQ=255)
                                                       tGGGGCAA.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCcgcggcg                                               >  1:5295386/1‑101 (MQ=255)
                                                        ggggCAA.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCcgcggcgc                                              >  2:8542997/1‑101 (MQ=255)
                                                         gggCAA.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCcgcggcgcg                                             <  1:1159090/101‑1 (MQ=255)
                                                            gaa.TGACCCGCAGGGGGTTGGGAA.GGCGCTGCGGCCTCCGG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGcc                                          <  2:7277363/100‑1 (MQ=255)
                                                            cAA.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGcc                                          <  1:3408884/101‑1 (MQ=255)
                                                             caacgccccGCACGGGCTTGGTAA.GGCCCTGCGGCACCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGcc                                          <  2:4217040/95‑1 (MQ=255)
                                                              a.TGCCCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGt                                        <  1:3631517/101‑1 (MQ=255)
                                                              a.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGt                                        <  2:3556072/101‑1 (MQ=255)
                                                                tgcccggCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTg                                       <  2:822108/95‑1 (MQ=255)
                                                                 gACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTGCCCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCggg                                       >  1:1629120/1‑99 (MQ=255)
                                                                 gACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGc                                      >  1:3812075/1‑101 (MQ=255)
                                                                  aCCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGcc                                     >  2:7585449/1‑101 (MQ=255)
                                                                   cccGCCGGGGCTTTTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCt                                    <  2:2868697/101‑1 (MQ=255)
                                                                   cccGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGGGGGAAGGCGCCCGCGGCGCGGCCGGGCCt                                    >  1:4210029/1‑101 (MQ=255)
                                                                   cccGCACGGGCTTGGGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCt                                    <  2:8621110/101‑1 (MQ=255)
                                                                    ccGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTg                                   >  1:944680/1‑101 (MQ=255)
                                                                     cGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGc                                  <  1:8837623/101‑1 (MQ=255)
                                                                     cGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CCACGGGGGGGCAGGGCTGGGTTCGGGCGGCAAGGCGCCCGCGGGGCGGCCGGGCCCGc                                  >  2:4485166/1‑101 (MQ=255)
                                                                       cACGGGCTTGTGCA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGGGCGGCCGGGCCCGcct                                >  1:6505725/1‑99 (MQ=255)
                                                                       cACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGCGCCCGCtt                                >  1:9067171/1‑101 (MQ=255)
                                                                        ccGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTa                               <  2:3004106/100‑1 (MQ=255)
                                                                        aCGGGCTGGGGAA.GGCGCTGGGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTa                               <  1:8062965/101‑1 (MQ=255)
                                                                         cGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTAc                              <  2:6291243/101‑1 (MQ=255)
                                                                         cGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCCGGCCTGCTTAc                              >  2:6055707/1‑101 (MQ=255)
                                                                           gggttGGGAA.GGCGCTGCGGCACCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGc                            <  1:499496/98‑1 (MQ=255)
                                                                             cTTGTGAA.GGCGCTGCGGCATCCTG..CCCCCCCC.CGACGGGAGGGCAGGGCTGGGTTCGGGGGGCAAGGCGCCCCCGGCGCGGCCGGGCCTGCTTACGcccg                         >  1:3760813/1‑99 (MQ=255)
                                                                              ttGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTg                         >  1:1449082/1‑101 (MQ=255)
                                                                              ttGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGGGCCGGCTTCCGCCTg                         >  2:9009296/1‑101 (MQ=255)
                                                                                gtgAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCa                       <  2:8844712/101‑1 (MQ=255)
                                                                                gtgAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGGGCCTGCTTACGccctca                       >  2:5405088/1‑97 (MQ=255)
                                                                                 tgAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAg                      >  1:748178/1‑101 (MQ=255)
                                                                                 ggAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAg                      <  2:3258721/100‑1 (MQ=255)
                                                                                   gaggggccTGCGGCTTCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGc                     <  2:1470121/94‑1 (MQ=255)
                                                                                       gcgcTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGcc                 <  1:7042719/101‑1 (MQ=255)
                                                                                        cgcTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCg                >  1:6712084/1‑101 (MQ=255)
                                                                                         gcTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCgg               <  1:8319571/101‑1 (MQ=255)
                                                                                           tGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGAc             <  2:6843170/101‑1 (MQ=255)
                                                                                            gCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCCGCGGCCGGCcc            >  2:7407777/1‑101 (MQ=255)
                                                                                             gggCAGCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCg           <  2:2489215/100‑1 (MQ=255)
                                                                                             cGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCg           <  2:2015784/101‑1 (MQ=255)
                                                                                             cGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCCGGGCCGCGCCTGGTTACGCCTGCTACGGCCGGCCCg           >  2:7876070/1‑101 (MQ=255)
                                                                                              ggCATCCTGCCCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCg           <  1:3146112/101‑1 (MQ=255)
                                                                                                 aTCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGAt       >  2:3432693/1‑101 (MQ=255)
                                                                                                   ccTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGAttt     <  2:7078139/101‑1 (MQ=255)
                                                                                                    cTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGATTTc    <  1:6187921/101‑1 (MQ=255)
                                                                                                    cTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGATTTc    <  1:4072119/101‑1 (MQ=255)
                                                                                                    cTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGATTTc    <  2:5344733/101‑1 (MQ=255)
                                                                                                     tG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGGGCCTGCTTACGCCCGCAGCGGCCGGGCCGCGATTTCg   >  1:5693875/1‑101 (MQ=255)
                                                                                                      g.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGATTTCGc  <  1:7177854/101‑1 (MQ=255)
                                                                                                      g.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGATTTCGc  >  2:7643966/1‑101 (MQ=255)
                                                                                                       |                                                                                                     
TCATTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAA.TGACCCGCACGGGCTTGTGAA.GGCGCTGCGGCATCCTG.CCCCCCCCC.CGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGATTTCGC  >  NC_014034/568701‑568901

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG