Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 586,916 +G 1.4% intergenic (‑144/+38) RCAP_rcc00548 ← / ← purL BolA family protein/phosphoribosylformylglycinamidine synthase II

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034586,9161.→G 1.4% 4.2 ‑4.4 0.0352intergenic (‑144/+38)RCAP_rcc00548/purLBolA family protein/phosphoribosylformylglycinamidine synthase II
Reads supporting (aligned to +/- strand):  new base (0/5):  ref base (204/143):  total (204/148)
Fisher's exact test strand distribution p-value = 1.26e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 409 total aligned reads displayed.

AGGCGACAAGAGCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.C.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGCTCTGCCAGCG  >  NC_014034/586816‑587012
                                                                                                      |                                                                                                 
agGCGACAAGAGCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.t                                                                                                    >  2:8492743/1‑101 (MQ=255)
  gCGACAAGAGCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.C.g                                                                                                >  2:3943902/1‑101 (MQ=255)
     aCAAGAGCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.C.gggg                                                                                             >  1:4207521/1‑101 (MQ=255)
      cAAGAGCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.C.ggggg                                                                                            >  1:1710379/1‑101 (MQ=255)
       aaGAGCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.C.gggggg                                                                                           >  2:7914807/1‑101 (MQ=255)
        agggCCAAGCGCGGGGGGGGTGTGCCAAGGGCCGCAGGGGGCGGGGGCCGGGGGGGGGACGGGGGTCGGGACGGGGGCAAGTGTCGCCGAGC.T.G.ggggggg                                                                                          <  2:8379847/98‑1 (MQ=255)
        agagCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.C.ggggggg                                                                                          <  1:367559/101‑1 (MQ=255)
             cAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.C.ggggggggcgct                                                                                     >  1:614589/1‑96 (MQ=255)
              aaGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCtt                                                                                     >  2:745637/1‑101 (MQ=255)
               aGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTc                                                                                    >  1:7420491/1‑101 (MQ=255)
                gcgccGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCt                                                                                   <  1:5104375/98‑1 (MQ=255)
                gCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCt                                                                                   >  2:6293372/1‑101 (MQ=255)
                gCACCGGGTGCGGGGGCCAGGGGACGCAGCGGGCAGGGGCCGCGGCGGGGGCGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.GGGGGGGGGCGCTTCt                                                                                   <  2:670388/101‑1 (MQ=255)
                 cACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTg                                                                                  >  2:2848360/1‑101 (MQ=255)
                  ccgggTGGCGGGTGCCAAGGGACGCAGCGGGCAGGGGCCGCGGCGGGGACGGGGGCCAGGCCGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTgc                                                                                 <  2:231766/98‑1 (MQ=255)
                  aCCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTgc                                                                                 >  2:5857479/1‑101 (MQ=255)
                  aCCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTgc                                                                                 >  2:907991/1‑101 (MQ=255)
                    gggTTGCGTGGGCCAAGTGACGCGGCGGGGAGGGGCCGGGGCGAGGACGGGGGTAAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTgccc                                                                               <  2:8106871/100‑1 (MQ=255)
                    cGGTGGCGTGTGCCAAGGGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTgccc                                                                               <  1:1285164/101‑1 (MQ=255)
                     gtttGGGTGTGCCAAGTCACGCGGCGGGCGGGGGCCGCGGCGAGGACGGGGGTCAGGGCGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTgcccg                                                                              <  2:3332212/99‑1 (MQ=255)
                     ggTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTgcccg                                                                              >  2:1224308/1‑101 (MQ=255)
                        tGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTgcccgccc                                                                           >  1:4158150/1‑101 (MQ=255)
                        tGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTgcccgccc                                                                           >  2:7683361/1‑101 (MQ=255)
                         gCGTGTGGCAAGGGACGCGGGGGGGGGGGGCCGCGGCGGGGACGGGGGGCAGGACGGGGGCAAGTGTCGCCGGGCGG.G.GGGGGGGCGCTTCTGCCCGcccc                                                                          <  1:1807227/101‑1 (MQ=255)
                         gCGTGTGCCAAGGGGCGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGcccc                                                                          <  2:691816/101‑1 (MQ=255)
                           gtgtGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCgg                                                                        >  2:3490111/1‑101 (MQ=255)
                           gtgtGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCgg                                                                        >  1:5198803/1‑101 (MQ=255)
                            tgtgCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGa                                                                       >  2:8624317/1‑101 (MQ=255)
                             gtgCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAg                                                                      <  1:1640987/101‑1 (MQ=255)
                             gtgCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAg                                                                      >  1:825361/1‑101 (MQ=255)
                             gtgCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAg                                                                      >  2:8743936/1‑101 (MQ=255)
                              tgCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGc                                                                     <  1:3944017/101‑1 (MQ=255)
                                cccagggACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCtt                                                                   <  1:1535020/95‑1 (MQ=255)
                                ccAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCtt                                                                   <  2:4437759/101‑1 (MQ=255)
                                   aGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCg                                                                >  1:4016810/1‑101 (MQ=255)
                                    gTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGt                                                               >  2:4708808/1‑101 (MQ=255)
                                    gTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGt                                                               >  1:8117023/1‑101 (MQ=255)
                                     tGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGtt                                                              >  1:4586491/1‑101 (MQ=255)
                                        gggcgCGGGCGGGGGCCGCGGGGAGGAGGGGGGGCAGGGCGGGGGCAAGGGGCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTc                                                           <  2:610431/97‑1 (MQ=255)
                                        cGCAGCGGGGGGGGGCCGCGGCGAGGGCGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTc                                                           <  1:3550014/101‑1 (MQ=255)
                                        cGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTc                                                           <  1:2348028/101‑1 (MQ=255)
                                         gCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCa                                                          <  1:8192127/101‑1 (MQ=255)
                                          cAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAg                                                         <  1:5834410/101‑1 (MQ=255)
                                          cAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAg                                                         >  1:2565378/1‑101 (MQ=255)
                                           aGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGc                                                        >  2:8314844/1‑101 (MQ=255)
                                               ggCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTc                                                    <  2:4841402/101‑1 (MQ=255)
                                               ggCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTc                                                    >  1:6751420/1‑101 (MQ=255)
                                                tccgatctcgcgGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCg                                                   <  1:7115613/93‑1 (MQ=255)
                                                gcgggggCCGCGGCGAGGGCGGGGGTCAGGGCGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCg                                                   <  1:729101/98‑1 (MQ=255)
                                                gCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCg                                                   <  2:6365603/101‑1 (MQ=255)
                                                gCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCg                                                   <  2:7163925/101‑1 (MQ=255)
                                                  aGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGAc                                                 >  2:5312203/1‑101 (MQ=255)
                                                   ggggCCGCGGGGGGGGGGGGGGTCGGGAGGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGAcg                                                <  1:8353652/101‑1 (MQ=255)
                                                   ggggCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGAcg                                                >  1:7903780/1‑101 (MQ=255)
                                                   ggggCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGAcg                                                >  1:6432876/1‑101 (MQ=255)
                                                   ggggCCCCGGGGCGGAGGGGGGTCAGGACGGGGGCACGTGGCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGAcg                                                <  2:4260387/101‑1 (MQ=255)
                                                   gggcgcgcgGCGAGGAGGGGGGGCAGGGCGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGAcg                                                <  2:5727957/96‑1 (MQ=255)
                                                    gggCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGAcgg                                               >  1:8631347/1‑101 (MQ=255)
                                                      gCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGAcggcg                                             >  1:3414804/1‑101 (MQ=255)
                                                      gCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGAcggcg                                             >  2:1381350/1‑101 (MQ=255)
                                                            gcgAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGgcggcggcg                                       <  1:6293612/101‑1 (MQ=255)
                                                             ggAGGAGGGGGGTCAGGACGGGGGCAAGGGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGa                                      <  2:3772970/100‑1 (MQ=255)
                                                              gAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGaa                                     >  1:8958492/1‑101 (MQ=255)
                                                                ggACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAgg                                   >  1:6597716/1‑101 (MQ=255)
                                                                 gACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGgc                                  <  1:6985804/101‑1 (MQ=255)
                                                                 gACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGgc                                  <  1:5572256/101‑1 (MQ=255)
                                                                   cGGGGGTCAGGACGGGGGCAGGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGgctg                                >  2:3867983/1‑99 (MQ=255)
                                                                   cGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGgcgg                                >  1:6914983/1‑101 (MQ=255)
                                                                    gggggTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGgcggg                               >  2:2534027/1‑100 (MQ=255)
                                                                      gggTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGgcggtgc                             >  2:7105671/1‑101 (MQ=255)
                                                                        gccAGGTCGGGGGCAAGTGTCGCCGGGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGgcggtgcgg                           <  2:2765082/99‑1 (MQ=255)
                                                                        gTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGgcggtgcgg                           >  2:5435840/1‑101 (MQ=255)
                                                                        gTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGgcggtgcgg                           >  1:4405599/1‑101 (MQ=255)
                                                                          cAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTa                         <  2:2005012/101‑1 (MQ=255)
                                                                          cAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTa                         >  1:8423553/1‑101 (MQ=255)
                                                                           aGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACTGCGGCGGCGAAGGCGGTGCGGTaa                        >  2:5184323/1‑101 (MQ=255)
                                                                           aGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTaa                        >  2:2799843/1‑101 (MQ=255)
                                                                            ggACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTaaa                       <  2:1729608/101‑1 (MQ=255)
                                                                             gACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAg                      >  1:1049906/1‑101 (MQ=255)
                                                                              gggggggCAAGTGTCGCCGGGC.TGG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGc                     <  1:48165/99‑1 (MQ=255)
                                                                                   ggCAAGTGTCGCCGAGC.TGG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCt                <  1:5061953/101‑1 (MQ=255)
                                                                                   ggCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCt                <  1:3332328/101‑1 (MQ=255)
                                                                                   ggCAAGTGGCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCt                <  1:2880499/101‑1 (MQ=255)
                                                                                    ggaaGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTg               <  2:1536953/99‑1 (MQ=255)
                                                                                    gCAGGTGGCGCCGAGG.TGG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTg               <  1:3199174/101‑1 (MQ=255)
                                                                                      aaGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAg             >  1:1732477/1‑101 (MQ=255)
                                                                                      aaGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAg             >  1:882503/1‑101 (MQ=255)
                                                                                      aaGGGTCGCCGAGC.TGG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAg             <  2:6794990/101‑1 (MQ=255)
                                                                                         tgtCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGGTGAGctc          >  2:6233865/1‑101 (MQ=255)
                                                                                         tgtCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGctc          >  2:4607928/1‑101 (MQ=255)
                                                                                          gtCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGctct         <  2:6594113/101‑1 (MQ=255)
                                                                                          gtCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGctct         >  2:3340435/1‑101 (MQ=255)
                                                                                          gtCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGctct         >  2:4072332/1‑101 (MQ=255)
                                                                                          gccGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGctct         <  1:2635050/99‑1 (MQ=255)
                                                                                           tCGGCGCGC.T.CGGGGGGGGCGCTGCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGCTCTg        <  1:2839141/101‑1 (MQ=255)
                                                                                              ccGTGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGCTCTGCCa     <  2:8199040/101‑1 (MQ=255)
                                                                                               cGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGCTCTGCCAg    <  1:943940/101‑1 (MQ=255)
                                                                                                gAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGCTCTGCCAgc   <  1:5312387/101‑1 (MQ=255)
                                                                                                gAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGCTCTGCCAgc   >  1:79813/1‑101 (MQ=255)
                                                                                                gAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGCTCTGCCAgc   >  2:2205943/1‑101 (MQ=255)
                                                                                                      |                                                                                                 
AGGCGACAAGAGCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.C.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGCTCTGCCAGCG  >  NC_014034/586816‑587012

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG