BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	586,917	+G	100%	intergenic (‑145/+37)	RCAP_rcc00548 ← / ← purL	BolA family protein/phosphoribosylformylglycinamidine synthase II

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	586,917	1	.→G	100.0%	49.0	359	intergenic (‑145/+37)	RCAP_rcc00548/purL	BolA family protein/phosphoribosylformylglycinamidine synthase II
Reads supporting (aligned to +/- strand): new base (179/161): ref base (2/17): total (181/178)
Fisher's exact test strand distribution p-value = 2.63e-04
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.27e-01

Only 100 of 408 total aligned reads displayed.


AGGCGACAAGAGCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.C.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGCTCTGCCAGCG  >  NC_014034/586816‑587012


gggcgcaaaGAGCCAAGCACCGGTTGCGGGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAA.TGTCGCCGAGC.T.c                                                                                                  <  2:6889133/94‑1 (MQ=255)


  gCGACAAGAGCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.C.g                                                                                                <  1:7096971/101‑1 (MQ=255)


     aCAAGAGCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.C.gggg                                                                                             >  1:4207521/1‑101 (MQ=255)


      cAAGAGCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.C.ggggg                                                                                            >  2:4664419/1‑101 (MQ=255)


      cAAGAGCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.C.ggggg                                                                                            >  1:1710379/1‑101 (MQ=255)


        agagCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.C.ggggggg                                                                                          >  1:6690250/1‑101 (MQ=255)


        agagCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.C.ggggggg                                                                                          <  1:367559/101‑1 (MQ=255)


             cAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.C.ggggggggcgct                                                                                     >  1:614589/1‑96 (MQ=255)


             cAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.C.ggggggggcgct                                                                                     >  2:2840339/1‑96 (MQ=255)


             cAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.C.ggggggggcgct                                                                                     >  2:8191778/1‑96 (MQ=255)


              aaGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCtt                                                                                     <  1:3197151/101‑1 (MQ=255)


               aGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTc                                                                                    >  1:7420491/1‑101 (MQ=255)


                gcgccGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCt                                                                                   <  1:5104375/98‑1 (MQ=255)


                  aCCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTgc                                                                                 >  2:2758873/1‑101 (MQ=255)


                  aCCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTgc                                                                                 >  2:907991/1‑101 (MQ=255)


                   ccGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTgcc                                                                                >  1:684582/1‑101 (MQ=255)


                    cGGTGGCGTGTGCCAAGGGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTgccc                                                                               <  1:1285164/101‑1 (MQ=255)


                     ggTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTgcccg                                                                              >  2:1224308/1‑101 (MQ=255)


                        tGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTgcccgccc                                                                           >  2:2796531/1‑101 (MQ=255)


                        tGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTgcccgccc                                                                           >  1:4158150/1‑101 (MQ=255)


                         gCGTGTGGCAAGGGACGCGGGGGGGGGGGGCCGCGGCGGGGACGGGGGGCAGGACGGGGGCAAGTGTCGCCGGGCGG.G.GGGGGGGCGCTTCTGCCCGcccc                                                                          <  1:1807227/101‑1 (MQ=255)


                         gCGTGTGCCAAGTGGCGCAGCGGGGAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGcccc                                                                          <  2:3928824/101‑1 (MQ=255)


                           gtgtGCCAAGTGACGCGGCGGGCAGGGGCCGCGGCGAGGAGGGGGGTCAGGACGGGGGCAGGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCgg                                                                        <  2:4603586/101‑1 (MQ=255)


                           gtgtGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCgg                                                                        >  2:8359608/1‑101 (MQ=255)


                           gtgtGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCgg                                                                        >  2:3490111/1‑101 (MQ=255)


                           gtgtGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCgg                                                                        >  1:5198803/1‑101 (MQ=255)


                           gtgtGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.G.CGGGGGGGGGGCGTCTGGCCGCCCCgg                                                                        >  2:4219849/1‑101 (MQ=255)


                            tgtgCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGa                                                                       >  2:5994952/1‑101 (MQ=255)


                            tgtgCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGa                                                                       >  2:8624317/1‑101 (MQ=255)


                             gtgCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAg                                                                      >  2:8743936/1‑101 (MQ=255)


                             gtgCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAg                                                                      <  1:1640987/101‑1 (MQ=255)


                             gtgCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAg                                                                      >  1:825361/1‑101 (MQ=255)


                              tgCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGc                                                                     <  1:3944017/101‑1 (MQ=255)


                               gCCAGGTGACGCAGGGGGCGGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCt                                                                    <  2:8455691/101‑1 (MQ=255)


                                cccagggACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCtt                                                                   <  1:1535020/95‑1 (MQ=255)


                                   aGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCg                                                                >  1:4016810/1‑101 (MQ=255)


                                    gTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGt                                                               >  1:8117023/1‑101 (MQ=255)


                                     tGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGtt                                                              >  1:4586491/1‑101 (MQ=255)


                                      gccGCAGCGGGCGGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGttt                                                             <  2:784837/99‑1 (MQ=255)


                                        cGCAGCGGGGGGGGGCCGCGGCGAGGGCGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTc                                                           <  1:3550014/101‑1 (MQ=255)


                                        cGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTc                                                           <  1:2348028/101‑1 (MQ=255)


                                         gcggCGGGCGGGGGCCGCGGCGAGGGCGGGGGTCAGGGCGGGGGCAAGTGTCGCCGAGC.TGG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCa                                                          <  1:8536870/98‑1 (MQ=255)


                                         gCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCa                                                          <  1:8192127/101‑1 (MQ=255)


                                          cAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAg                                                         >  1:2565378/1‑101 (MQ=255)


                                          cAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAg                                                         <  1:5834410/101‑1 (MQ=255)


                                              gggCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCtt                                                     >  1:6952253/1‑101 (MQ=255)


                                               ggCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTc                                                    >  2:2290156/1‑101 (MQ=255)


                                               ggCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTc                                                    <  2:800088/101‑1 (MQ=255)


                                                gcgggggGCGGGGCGAGGAGGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCg                                                   <  2:4763089/98‑1 (MQ=255)


                                                gcgggggCCGCGGCGAGGGCGGGGGTCAGGGCGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCg                                                   <  1:729101/98‑1 (MQ=255)


                                                gCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCg                                                   <  2:7163925/101‑1 (MQ=255)


                                                 ccggggCCGCGGGGAGGCGGCGGGTCAGGGCGGGGGCAAGTTGCGCCGAGC.G.C.GGGGGGGCGCTTATGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGAc                                                 <  1:8415622/99‑1 (MQ=255)


                                                  aGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGAc                                                 >  2:5312203/1‑101 (MQ=255)


                                                  aGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGAc                                                 >  2:5156902/1‑101 (MQ=255)


                                                   ggggCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGAcg                                                >  1:7903780/1‑101 (MQ=255)


                                                   ggggCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGAcg                                                >  1:6432876/1‑101 (MQ=255)


                                                   gggcgcgcgGCGAGGAGGGGGGGCAGGGCGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGAcg                                                <  2:5727957/96‑1 (MQ=255)


                                                      gcgggggcgCGGAGGGGGGTCGGGACGGGGGCAAGTGTCGCCGAGC.G.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGAcggcg                                             <  2:320042/96‑1 (MQ=255)


                                                      gCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGAcggcg                                             >  1:3414804/1‑101 (MQ=255)


                                                      gCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGAcggcg                                             >  2:1381350/1‑101 (MQ=255)


                                                        cgcgGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGAcggcggc                                           <  2:5811589/101‑1 (MQ=255)


                                                          cggcgAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGAcggcggcgg                                         <  2:4352806/101‑1 (MQ=255)


                                                            gcgAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGgcggcggcg                                       >  2:2193195/1‑101 (MQ=255)


                                                            gcgAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGgcggcggcg                                       <  1:6293612/101‑1 (MQ=255)


                                                             ggAGGAGGGGGGTCAGGACGGGGGCAAGGGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGa                                      <  2:3772970/100‑1 (MQ=255)


                                                               aGGACGGGGGCCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGGGGCGGCGAAg                                    >  1:4996424/1‑101 (MQ=255)


                                                                ggACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAgg                                   >  1:6597716/1‑101 (MQ=255)


                                                                 gACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGgc                                  <  2:6345384/101‑1 (MQ=255)


                                                                 gACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGgc                                  <  1:5572256/101‑1 (MQ=255)


                                                                   cGGGGGTCAGGACGGGGGCAGGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGgctg                                >  2:3867983/1‑99 (MQ=255)


                                                                     ggggTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGgcgggg                              >  2:2488407/1‑99 (MQ=255)


                                                                        gTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGgcggtgcgg                           >  2:5435840/1‑101 (MQ=255)


                                                                        gTCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGgcggtgcgg                           >  1:4405599/1‑101 (MQ=255)


                                                                         tCAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGgcggtgcggt                          >  2:6650075/1‑101 (MQ=255)


                                                                          cAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGGGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTa                         >  1:8918099/1‑101 (MQ=255)


                                                                          cAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTa                         >  2:6291882/1‑101 (MQ=255)


                                                                          cAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTa                         <  2:2005012/101‑1 (MQ=255)


                                                                          cAGGACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTa                         >  2:6586168/1‑101 (MQ=255)


                                                                            ggACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTaaa                       >  2:4033851/1‑101 (MQ=255)


                                                                            ggACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTaaa                       <  2:1729608/101‑1 (MQ=255)


                                                                             gACGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAg                      >  1:1049906/1‑101 (MQ=255)


                                                                              gcGGGGGGAAGTGTCGCGGAGC.TGG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGc                     <  2:6791850/100‑1 (MQ=255)


                                                                               cGGGGGCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCg                    >  2:7678295/1‑101 (MQ=255)


                                                                                 ggggCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTg                  <  2:8285208/101‑1 (MQ=255)


                                                                                   ggCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCt                <  1:3332328/101‑1 (MQ=255)


                                                                                   ggCAAGTGGCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCt                <  1:2880499/101‑1 (MQ=255)


                                                                                    ggaaGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTg               <  2:1536953/99‑1 (MQ=255)


                                                                                    gCAAGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTg               <  1:8741655/101‑1 (MQ=255)


                                                                                      aaGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAg             <  2:6988765/101‑1 (MQ=255)


                                                                                      aaGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAg             >  1:1732477/1‑101 (MQ=255)


                                                                                       aGTGTCGCCGAGC.TGG.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGc            <  2:6205128/101‑1 (MQ=255)


                                                                                       aGTGTCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGc            >  2:7387598/1‑101 (MQ=255)


                                                                                          gtCGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGctct         >  2:3340435/1‑101 (MQ=255)


                                                                                          gccGCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGctct         <  1:2635050/99‑1 (MQ=255)


                                                                                           tCGGCGCGC.T.CGGGGGGGGCGCTGCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGCTCTg        <  1:2839141/101‑1 (MQ=255)


                                                                                             gCCGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGCTCTGcc      <  1:8315207/101‑1 (MQ=255)


                                                                                             gCCGAGC.G.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCCAAGGCGGTGCGGTAAAGCGTGCTGAGCTCTGcc      <  1:4771689/101‑1 (MQ=255)


                                                                                               cGAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGCTCTGCCAg    <  1:943940/101‑1 (MQ=255)


                                                                                                gAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGCTCTGCCAgc   <  1:5312387/101‑1 (MQ=255)


                                                                                                gAGC.T.CGGGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGCTCTGCCAgc   >  1:79813/1‑101 (MQ=255)


AGGCGACAAGAGCCAAGCACCGGTTGCGTGTGCCAAGTGACGCAGCGGGCAGGGGCCGCGGCGAGGACGGGGGTCAGGACGGGGGCAAGTGTCGCCGAGC.T.C.GGGGGGGCGCTTCTGCCCGCCCCGGAGCTTGCGTTTTCAGCCTTCGACGGCGGCGGCGAAGGCGGTGCGGTAAAGCGTGCTGAGCTCTGCCAGCG  >  NC_014034/586816‑587012

Base quality scores:


ATCG

< 3 ≤


ATCG

< 15 ≤


ATCG

< 24 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG