Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 842,029 +G 1.7% coding (1673/2220 nt) hypF → hydrogenase maturation protein HypF

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034842,0291.→G 1.7% 6.8 ‑4.4 ‑0.0291coding (1673/2220 nt)hypFhydrogenase maturation protein HypF
Reads supporting (aligned to +/- strand):  new base (5/0):  ref base (117/169):  total (122/169)
Fisher's exact test strand distribution p-value = 1.23e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.80e-01

Only 100 of 370 total aligned reads displayed.

GGCTTCGAGCGGGTGGCCTGGCTGAAACCCGCGCCCCTGATCGGCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGCGCCGCGCGATCTGGCCCGGCAGCTGGC.GGCCAAGGGGATCAACGCGCCGCTGTCGTCCAGC  >  NC_014034/841929‑842129
                                                                                                     |                                                                                                      
    tCGAGCGGGTGGCCTGGCTGAAACCCGCGCCCCTGATCGGCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.gggg                                                                                                    >  1:8380241/1‑101 (MQ=255)
       aGCGGGTGGCCTGGCTGAAACCCGCGCCCCTGATCGGCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGG.CGGGcgt                                                                                                 >  2:4517949/1‑99 (MQ=255)
       aGCGGGTGGCCTGGCTGAAACCCGCGCCCCTGATCGGCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCtt                                                                                                 <  2:7793644/101‑1 (MQ=255)
       aGCGGGTGGCCTGGCTGAAACCCGCGCCCCTGATCGGCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCtt                                                                                                 <  1:6144249/101‑1 (MQ=255)
        gCGGGTGGCCTGGCTGAAACCCGCGCCCCTGATCGGCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCttt                                                                                                <  1:8162950/101‑1 (MQ=255)
         cGGGTGGCCTGGCTGAAACCCGCGCCCCTGATCGGCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTc                                                                                               <  1:3976343/101‑1 (MQ=255)
         cGGGTGGCCTGGCTGAAACCCGCGCCCCTGATCGGCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTc                                                                                               <  1:8598819/101‑1 (MQ=255)
         cGGGTGGCCTGGCTGAAACCCGCGCCCCTGATCGGCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCGTTc                                                                                               >  1:4533865/1‑101 (MQ=255)
         cGGGTGGCCGGGCTGAAACCCGCGCCCCTGTTCGGCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTc                                                                                               <  2:9055050/101‑1 (MQ=255)
          gggTGGCCTGGCTGAAACCCGCGCCCCTGATCGGCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCgtttc                                                                                              >  1:6510614/1‑99 (MQ=255)
          gggTGGCCTGGCTGAAACCCGCGCCCCTGATCGGCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTcc                                                                                              <  1:8876470/101‑1 (MQ=255)
          gggTGGCCTGGCTGAAACCCGCGCCCCTGATCGGCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTcc                                                                                              <  2:6289985/101‑1 (MQ=255)
            gTGGCCTGGCTGAAACCCGCGCCCCTGATCGGCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGGCGCGGC.cggggcctcgcc                                                                                            >  2:359137/1‑93 (MQ=255)
             tGGCCTGGCTGAAACCCGCGCCCCTGATCGGCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTCCGAt                                                                                           <  1:1302580/101‑1 (MQ=255)
             tGGCCTGGCTGAAACCCGCGCCCCTGATCGGCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTCCGAt                                                                                           <  1:5910669/101‑1 (MQ=255)
             tGGCCTGGCTGAAACCCGCGCCCCTGATCGGCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGAttttccat                                                                                           >  2:6217326/1‑96 (MQ=255)
                ccTGGCTGAAACCCGCGCCCCTGATCGGCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTg                                                                                        <  2:1728718/101‑1 (MQ=255)
                  tGGCTGAAACCCGCGCCCCTGATCGGCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGGGTTTCGGTCTGGc                                                                                      >  1:1898660/1‑101 (MQ=255)
                  tGGCTGAAACCCGCGCCCCTGATCGGCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCttttcccgctggc                                                                                      >  1:1629061/1‑91 (MQ=255)
                   ggCTGAAACCCGCGCCCCTGATCGGCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGcc                                                                                     <  2:7594095/101‑1 (MQ=255)
                   ggCTGAAACCCGCGCCCCTGATCGGCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGcc                                                                                     <  1:1092299/101‑1 (MQ=255)
                    gCTGAAACCCGCGCCCCTGATCGGCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGGCTTTCGGTCTgggcc                                                                                    >  2:7261910/1‑98 (MQ=255)
                    gCTGAAACCCGCGCCCCTGATCGGCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCg                                                                                    <  2:2628695/101‑1 (MQ=255)
                     cTGAAACCCGCGCCCCTGATCGGCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGAGCTGGTGCGGCTTGAAGCGGC.GGGGGCTTCCGATATGGcccg                                                                                   >  2:458636/1‑99 (MQ=255)
                      tGAAACCCGCGCCCCTGATCGGCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGAc                                                                                  <  2:7471750/101‑1 (MQ=255)
                       gAAACCCGCGCCCCTGATCGGCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTTCGATCTGGCCGAcc                                                                                 >  1:5489099/1‑101 (MQ=255)
                       gAAACCCGCGCCCCTGATCGGCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGCC.GGGGATTTCCGCGCTTGCCgcgc                                                                                 >  2:16053/1‑98 (MQ=255)
                        aaaCCCGCGCCCCTGATCGGCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTTCGATCTGGccccgcg                                                                                >  1:1492558/1‑96 (MQ=255)
                        aaaCCCGCGCCCCTGATCGGCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.g                                                                               <  2:5259526/101‑1 (MQ=255)
                        aaaCCCGCGCCCCTGATCGGCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.CGGGGCTTCCGATCTGGACCACC.g                                                                               >  2:1599844/1‑101 (MQ=255)
                         aaCCCGCGCCCCTGATCGGCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGGGTTTCGATGTGGCCCAcccg                                                                               >  2:3510367/1‑99 (MQ=255)
                         aaCCCGCGCCCCTGATCGGCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGGGTTCCCCGCTCGGCGACC.gg                                                                              >  2:5101444/1‑101 (MQ=255)
                         aaCCCGCGCCCCTGATCGGCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.gg                                                                              <  1:8014954/101‑1 (MQ=255)
                             cgcgCCCCTGATCGGCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCttt                                                                          <  2:6839523/101‑1 (MQ=255)
                              gcgcCCCTGATCGGCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCtttt                                                                         <  2:6725553/101‑1 (MQ=255)
                               cgcCCCTGATCGGCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCTTTTc                                                                        <  1:8987561/101‑1 (MQ=255)
                                gcCCCTGATCGGCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGGTTTTCGGTCTGGGCGACCGGGCTTTTc                                                                        >  1:7651583/1‑101 (MQ=255)
                                gcCCCTGATCGGCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGGTTTCCGATCTGGCCGACC.CGCTTTTcc                                                                       >  2:8404084/1‑101 (MQ=255)
                                gcCCCTGATCGGCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTCCCAGCCTGCCGGCC.CGCCTTTcc                                                                       >  2:3980599/1‑101 (MQ=255)
                                  cccTGATCGGCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGCGGGGGGTTTCCGACTGGGCGGGCC.GGGTTTTtcc                                                                      >  2:407651/1‑101 (MQ=255)
                                  cccTGATCGGCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCTTTTcccc                                                                     <  2:427924/101‑1 (MQ=255)
                                   ccTGATCGGCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCCTTCCGGTCTGGCCCACC.GGCTTTTtcccc                                                                    >  2:1934741/1‑100 (MQ=255)
                                    cTGATCGTCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCcgc                                                                   <  2:7048398/101‑1 (MQ=255)
                                    cTGATCGGCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCcgc                                                                   <  1:4899467/101‑1 (MQ=255)
                                       aTCGGCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGGTTTTCGGGCTGGCCGACC.CGGTTTTTCCCccggc                                                                >  1:3255219/1‑101 (MQ=255)
                                           gcggcgACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGcgccg                                                            <  2:606181/101‑1 (MQ=255)
                                             ggcgACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGCGCcgcg                                                          <  2:8900446/101‑1 (MQ=255)
                                                gACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTCCGCTCTGGCCGTAC.GGCTTTTTCCCGCGGCGCCCGGCGa                                                       >  1:3788067/1‑101 (MQ=255)
                                                  ccGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGCGCCGCGCGATc                                                     <  2:496099/101‑1 (MQ=255)
                                                    gggCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGCGCCGCGCGATCTg                                                   <  2:8203799/101‑1 (MQ=255)
                                                    gggCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGCGCCGCGCGATCTg                                                   <  2:5417026/101‑1 (MQ=255)
                                                      gcgcAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGCGCCGCGCGATCTGGc                                                 <  2:1530318/101‑1 (MQ=255)
                                                       ggcAGATCGAGCTCTGGCGCAATGCGCTGGTGGGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGCGCCGCGCGATCTGGcc                                                <  2:2727113/100‑1 (MQ=255)
                                                       cgcAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCTTTTTCCCGCGGCGCCGCGCGATCTGGcc                                                >  2:6677186/1‑101 (MQ=255)
                                                         cAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCCCGGCGCCGCGCGATCTGGCCCg                                              >  1:3016545/1‑101 (MQ=255)
                                                          aGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGCGCCGCGCGATCTGGCCCgg                                             <  1:2206715/101‑1 (MQ=255)
                                                          aGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGCGCCGCGCGATCTGGCCCgg                                             >  1:6828591/1‑101 (MQ=255)
                                                          aGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGCGCCGCGCGATCTGGCCCgg                                             >  2:7655879/1‑101 (MQ=255)
                                                           gATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGTTTTTCCCCGCGGCGCCGCGCGATCTGGCCCGGc                                            <  1:4773073/101‑1 (MQ=255)
                                                              cGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGGCC.GGCTTTTCCCCGCGGCGCCGCGCGATCTGGccccggcgg                                         >  2:3696205/1‑95 (MQ=255)
                                                               gAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGCGCCGCGCGATCTGGCCCGGCAGCt                                        >  1:6369616/1‑101 (MQ=255)
                                                                aGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGCGCCGCGCGATCTGGCCCGGCAGCTg                                       <  2:8093643/101‑1 (MQ=255)
                                                                 gCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTTCCATCTGGCCGACC.GGCTTTTCCCCCCGGCGCCGCGCGAGCCGGCCCCGcgccccg                                      >  2:284144/1‑95 (MQ=255)
                                                                  cccTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGGGCCGCGCGGTCTGGCCCGGCaaccggg                                     >  2:8815141/1‑95 (MQ=255)
                                                                  cccTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGCGCCGCGCGATCTGGCCCGGCAGCTggc                                     <  1:1405308/101‑1 (MQ=255)
                                                                   ccTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGCGCCGCGCGGTCTGGCCCGGCCGCTggc.g                                   >  1:9124703/1‑101 (MQ=255)
                                                                       gcgcAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGCGCCGCGCGATCTGGCCCGGCAGCTGGC.GGCCa                               <  1:2710581/101‑1 (MQ=255)
                                                                          cAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGCGCCGCGCGATCTGGCCCGGCAGCTGGC.GGCCAAgg                            >  1:4363113/1‑101 (MQ=255)
                                                                           aaTGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCCTTTCCCCGCGGCGCCGCGCGGTCTGGCCCGGCAGCCGGC.GGGCCAggg                           >  1:7284892/1‑101 (MQ=255)
                                                                             tGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGCGCCGCGCGATCTGGCCCGGCAGCTGGC.GGCCAAGGGGa                         <  2:6546783/101‑1 (MQ=255)
                                                                              gcgcTGGTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGGGCCGCGCGATCTGGCCCGGCAGGCGGCGGGCCAAGGGGa                         >  2:867686/1‑101 (MQ=255)
                                                                              gcgcTGGTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGCGCCGCGCGATCTGGGCCGGCAGCTGGC.GGGCAAgggggt                        >  2:1199073/1‑99 (MQ=255)
                                                                              gcgcTGGTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGCGCCGCGCGATCTGGCCCGGCAGCTGGC.GGCCAAGGGGAt                        <  2:5183127/101‑1 (MQ=255)
                                                                                gcTGGTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGCGCCGCGCGATCTGGCCCGGCAGCTGGC.GGCCAAGGGGATCa                      <  1:1829562/101‑1 (MQ=255)
                                                                                 cTGGTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGCGCCGCGCGATCTGGCCCGGCAGCTGGC.GGCCAAGGGGATCaa                     <  2:128530/101‑1 (MQ=255)
                                                                                  tGGTGCGGCTTGACGCGGC.GTGGCTTTCCGATCTGGCCGATC.GGCTTTTCCCCGCGGCGCCGCGCGATCTGGCCCGGCAGCTGTC.GGCCAAGGGGATCAAc                    <  2:4763690/101‑1 (MQ=255)
                                                                                  tGGTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGGGCCGCGCGGTCTGGCCCGGCCGCTGGC.GGCCCAGGGGATGAAc                    >  1:288036/1‑101 (MQ=255)
                                                                                  tGGTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGCGCCGCGCGATCTGGCCCGGCAGCTGGC.GGCCAAGGGGATCAAc                    <  1:809590/101‑1 (MQ=255)
                                                                                  tGGTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGCGCCGCGCGATCTGGCCCGGCAGCTGGC.GGCCAAGGGGATCAAc                    <  2:3075914/101‑1 (MQ=255)
                                                                                  tGGTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGCGCCGCGCGATCTGGCCCCGCAGCTGGC.GGGCCAGGGGGTCAAc                    >  1:3062927/1‑101 (MQ=255)
                                                                                   ggTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGCGCCGCGCGATCTGGCCCGGCAGCTGGC.GGCCCAGGGGGTCCAcc                   >  2:5477681/1‑100 (MQ=255)
                                                                                   ggTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGCGCCGCGCGATCTGGCCCGGCAGCTGGC.GGCCAAGGGGGTCaaag                   >  1:6723894/1‑99 (MQ=255)
                                                                                   ggTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGCGCCGCGCGATCTGGCCCGGCAGCTGGC.GGCCAAGGGGGTCCAcg                   >  1:723954/1‑101 (MQ=255)
                                                                                    gTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGCGCCGCGCGATCTGGCCCGGCAGCTGGC.GGCCAAGGGGATCAAcgc                  <  2:1829967/101‑1 (MQ=255)
                                                                                        ggCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGCGCCGCGCGATCTGGCCCGGCAGCTGGC.GGCCAAGGGGATCAACGcgccg              <  2:8464214/101‑1 (MQ=255)
                                                                                        ggCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGCGCCGCGCGATCTGGCCCGGCAGCTGGC.GGCCAAGGGGATCAACGcgccg              <  2:2561071/101‑1 (MQ=255)
                                                                                        ggCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCCCGGCGCCGCGCGATCTGGCCCGGCAGCTGGC.GGCCAAGGGGGTCAACGcgccc              >  1:3665806/1‑100 (MQ=255)
                                                                                          cTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGCGCCGCGCGATCTGGCCCGGCAGCTGGC.GGCCAAGGGGATCAACGCGCCGCt            <  1:8459294/101‑1 (MQ=255)
                                                                                              tcgcgGC.GGGGCTTTCCGATCTGGCCGACC.CGCTTTTCCCCGCGGCGCCGCGCGATCTGGCCCGGCAGCTGGC.GGCCAAGGGGATCAACGCGCCGCTgtcg        <  1:664698/100‑1 (MQ=255)
                                                                                              aCGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGCGCCGCGCGATCTGGCCCGGCAGCTGGC.GGCCAAGGGGATCAACGCGCCGCTgtcg        <  1:2461783/101‑1 (MQ=255)
                                                                                                 cggc.ggGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGCGCCGCGCGATCTGGCCCGGCAGCTGGC.GGCCAAGGGGATCAACGCGCCGCTGTCGTcc     <  1:5147917/101‑1 (MQ=255)
                                                                                                 cggc.ggGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGCGCCGCGCGATCTGGCCCGGCAGCTGGC.GGCCAAGGGGATCAACGCGCCGCTGTCGTcc     <  1:3397249/101‑1 (MQ=255)
                                                                                                 cggc.ggGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGCGCCGCGCGATCTGGCCCGGCAGCTGGC.GGCCAAGGGGATCAACGCGCCGCTGTCGTcc     <  1:8228633/101‑1 (MQ=255)
                                                                                                 cggc.ggGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGCGCCGCGCGATCTGGCCCGGCAGCTGGC.GGCCAAGGGGATCAACGCGCCGCCGGCGTcc     >  2:2221588/1‑101 (MQ=255)
                                                                                                  ggc.ggGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGCGCCGCGCGATCTGGCCCGGCAGCTGGC.GGCCAAGGGGATCAACGCGCCGCTGTCGTCCa    <  1:6954191/101‑1 (MQ=255)
                                                                                                  ggc.ggGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGCGCCGCGCGATCTGGCCCGGCAGCTGGC.GGCCAAGGGGATCAACGCGCCGCTGTCGTCCa    <  2:101021/101‑1 (MQ=255)
                                                                                                  ggc.ggGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGCGCCGCGCGATCTGGCCCGGCAGCTGGC.GGCCAAGGGGATCAACGCGCCGCTGTCGTCCa    <  1:407659/101‑1 (MQ=255)
                                                                                                   gc.ggGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGCGCCGCGCGATCTGGCCCGGCAGCGGGC.GGCCAAGGGGATCAACGCGCCGCCGTCGCcccg   >  1:5320175/1‑99 (MQ=255)
                                                                                                    c.ggGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGCGCCGCGCGATCTGGCCCGGCAGCTGGC.GGCCAAGGGGATCAACGCGCCGCTGTCGTCCAgc  >  1:6287520/1‑101 (MQ=255)
                                                                                                    c.ggGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGCGCCGCGCGATCTGGCCCGGCAGCTGGC.GGCCAAGGGGATCAACGCGCCGCTGTCGTCCAgc  >  1:600161/1‑101 (MQ=255)
                                                                                                     |                                                                                                      
GGCTTCGAGCGGGTGGCCTGGCTGAAACCCGCGCCCCTGATCGGCGGCGACCGGGCGCAGATCGAGCCCTGGCGCAATGCGCTGGTGCGGCTTGACGCGGC.GGGGCTTTCCGATCTGGCCGACC.GGCTTTTCCCCGCGGCGCCGCGCGATCTGGCCCGGCAGCTGGC.GGCCAAGGGGATCAACGCGCCGCTGTCGTCCAGC  >  NC_014034/841929‑842129

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG