Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 904,934 +GC 100% intergenic (+38/‑158) RCAP_rcc00821 → / → murB hypothetical protein/UDP‑N‑acetylmuramate dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034904,9341.→G100.0% 92.6 304intergenic (+38/‑158)RCAP_rcc00821/murBhypothetical protein/UDP‑N‑acetylmuramate dehydrogenase
Reads supporting (aligned to +/- strand):  new base (131/141):  ref base (5/26):  total (137/167)
Fisher's exact test strand distribution p-value = 5.28e-04
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.41e-01
*NC_014034904,9342.→C100.0% 97.6 304intergenic (+38/‑158)RCAP_rcc00821/murBhypothetical protein/UDP‑N‑acetylmuramate dehydrogenase
Reads supporting (aligned to +/- strand):  new base (131/141):  ref base (6/26):  total (137/167)
Fisher's exact test strand distribution p-value = 1.34e-03
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.45e-01

Only 100 of 328 total aligned reads displayed.

GGGCTGAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTCTTTTTG.GCT..GC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCTTGCGGACG  >  NC_014034/904834‑905025
                                                                                                      ||                                                                                             
gggCTGAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCT..g                                                                                              <  1:7698736/101‑1 (MQ=255)
 ggCTGAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCT..Gc                                                                                             >  1:4960954/1‑101 (MQ=255)
  gCTGAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCT..Gcg                                                                                            <  1:7843479/101‑2 (MQ=255)
    tgggCGTGCTGCGCTGGCCGTTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCT..Gcgcc                                                                                          <  2:9119945/98‑4 (MQ=37)
    tGAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCT..Gcgcc                                                                                          >  1:9056544/1‑98 (MQ=255)
    tGAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCT..Gcgcc                                                                                          >  1:7574544/1‑98 (MQ=255)
    tGAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCT..Gcgcc                                                                                          >  1:3196038/1‑98 (MQ=255)
     gggCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCCACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCT..Gcgcca                                                                                         <  2:3230511/99‑5 (MQ=37)
     gAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCT..Gcgcca                                                                                         >  1:1932389/1‑97 (MQ=255)
     gAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCT..Gcgcca                                                                                         <  1:5169030/101‑5 (MQ=255)
         gTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCT..Gcgccaaaat                                                                                     <  2:6617455/101‑9 (MQ=37)
           gctgcGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTc                                                                                   >  1:1668536/1‑101 (MQ=25)
           gctgcGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCT..Gcgcccaaattc                                                                                   >  2:1149862/1‑91 (MQ=37)
             tgcGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCAc                                                                                 <  1:5829256/101‑1 (MQ=25)
              gcgcTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGGCCCCCTCTTTTTGCTGC..GC..CAAAATTCAcc                                                                                >  1:8156908/1‑101 (MQ=37)
                gcTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCAcccc                                                                              <  2:6226201/101‑1 (MQ=25)
                  tGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCAcccccc                                                                            >  2:5344473/1‑101 (MQ=21)
                  tGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCAcccccc                                                                            <  2:430093/101‑1 (MQ=21)
                  tGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCAcccccc                                                                            <  1:3047500/101‑1 (MQ=21)
                  tGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCAcccccc                                                                            <  2:2425389/101‑1 (MQ=21)
                   ggCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCg                                                                           <  2:6491659/101‑1 (MQ=21)
                   ggCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCg                                                                           <  2:7270485/101‑1 (MQ=21)
                   ggCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCg                                                                           >  1:8301583/1‑101 (MQ=21)
                   ggCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCg                                                                           <  2:1850961/101‑1 (MQ=21)
                       ggTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGt                                                                       >  1:5434029/1‑101 (MQ=21)
                       ggTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCCGCGt                                                                       >  2:6108943/1‑101 (MQ=21)
                         tGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTAt                                                                     >  2:1956973/1‑101 (MQ=21)
                          gcggcgGCTCTTTGCCGCGATGCACCGGCGCGTCTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTAtt                                                                    <  2:6760804/101‑1 (MQ=21)
                             gcggcTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGc                                                                 >  2:2237828/1‑101 (MQ=21)
                               ggcTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCAc                                                               <  2:8376505/101‑1 (MQ=21)
                                gcTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCAcc                                                              <  1:5566482/101‑1 (MQ=21)
                                    tttGCCGCGCTGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGa                                                          <  2:898862/101‑1 (MQ=18)
                                      tGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGaaa                                                        >  1:8630270/1‑101 (MQ=21)
                                      tGCAGCGAGGCGCCGGGGCGAATGAGAGTGGAGCAGGAGTGCGCTGCCCCCCTC.TTTTG.GCT..GCGGGAAAATTCGCCCCCCGGCGTATTTGCACCAGGaaa                                                        <  2:7987612/101‑1 (MQ=12)
                                       gCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGaaaa                                                       <  1:2834680/101‑1 (MQ=21)
                                        cctgcaTGCCCCGGCGGTGCTGAGGGCGGGGCATGGGCGCTCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTTCCCCCCCGGCGTATTTGCACCAGGaaaaa                                                      <  1:4244462/96‑1 (MQ=9)
                                           cgATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGcc                                                   <  1:6103679/101‑1 (MQ=21)
                                             aTGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCg                                                 >  1:6414716/1‑101 (MQ=21)
                                             aTGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCg                                                 >  2:4816615/1‑101 (MQ=21)
                                              tGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCgg                                                <  2:388182/101‑1 (MQ=21)
                                               gCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGc                                               >  1:669985/1‑101 (MQ=21)
                                               gCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGc                                               >  2:7600760/1‑101 (MQ=21)
                                                 aCCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGt                                             >  2:7135625/1‑101 (MQ=21)
                                                  cgatctcgACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGtt                                            <  1:403173/95‑1 (MQ=18)
                                                  ccGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGtt                                            <  1:2153226/101‑1 (MQ=21)
                                                   cGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGttt                                           >  2:1453528/1‑101 (MQ=21)
                                                   cGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGttt                                           <  2:5440500/101‑1 (MQ=21)
                                                    ggCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGtttt                                          <  2:6921449/101‑1 (MQ=21)
                                                    ggCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGtttt                                          <  1:5319199/101‑1 (MQ=21)
                                                    ggCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGCCNNATNTGCACCAGGAAAAAGCCCGGCGtttt                                          >  1:4558149/1‑101 (MQ=18)
                                                      cgcgACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTc                                        <  1:3780138/101‑1 (MQ=21)
                                                       gcgACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTcc                                       >  2:2777989/1‑101 (MQ=21)
                                                       gcgACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTcc                                       <  1:1469244/101‑1 (MQ=21)
                                                           cTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGt                                   >  2:4752186/1‑101 (MQ=21)
                                                           cTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGt                                   <  1:6237732/101‑1 (MQ=21)
                                                             gAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCg                                 >  1:7996015/1‑101 (MQ=21)
                                                             gAGGGCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCg                                 >  2:2575940/1‑101 (MQ=21)
                                                               gggCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATACACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGaa                               <  1:1969777/101‑1 (MQ=21)
                                                                 gCGGGGCCGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAAt                             <  2:7685817/101‑1 (MQ=21)
                                                                 gCGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGCAAt                             <  1:8812868/101‑1 (MQ=21)
                                                                  cGGGGCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATa                            >  1:8504467/1‑101 (MQ=21)
                                                                   ggggCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATAc                           >  2:556017/1‑101 (MQ=21)
                                                                    gggCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACg                          <  2:5894269/101‑1 (MQ=21)
                                                                    gggCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACg                          >  1:747450/1‑101 (MQ=21)
                                                                    gggCAGGGGGGCGCTCCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCCCCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACg                          <  1:4369771/101‑1 (MQ=21)
                                                                     ggCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGc                         >  2:7463629/1‑101 (MQ=21)
                                                                      gCAGGGGGTCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGcc                        <  2:3175399/101‑1 (MQ=21)
                                                                      gCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGcc                        >  2:6394148/1‑101 (MQ=21)
                                                                      gCAGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCAGGCGTATTGGCACCAGGAAAAAGCCCGGCGGTTCTACTGGTCGACATAGGcc                        >  2:8579417/1‑101 (MQ=17)
                                                                        aGGGGGGCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCgg                      >  1:3663202/1‑101 (MQ=25)
                                                                         ggggggCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCggg                     >  1:5735476/1‑101 (MQ=25)
                                                                          gggggCGCTGCCCCCCTCTTTTGG.GTGC.GC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCgggg                    >  2:4387889/1‑101 (MQ=25)
                                                                            gggCGCTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCgggggg                  <  1:4109746/101‑1 (MQ=25)
                                                                                gcTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCAGCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTc              >  1:4773161/1‑101 (MQ=37)
                                                                                gcTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTc              >  2:8792607/1‑101 (MQ=37)
                                                                                 cTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTcc             <  1:3140487/101‑1 (MQ=37)
                                                                                 cTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTcc             <  2:8504087/101‑1 (MQ=37)
                                                                                 cTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTcc             >  2:2000619/1‑101 (MQ=37)
                                                                                 cTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTcc             >  2:3011233/1‑101 (MQ=37)
                                                                                 cTGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTcc             <  2:3061526/101‑1 (MQ=37)
                                                                                  tGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCg            <  2:8231379/101‑1 (MQ=37)
                                                                                  tGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCg            >  1:1746566/1‑101 (MQ=37)
                                                                                  tGCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCg            <  2:4076661/101‑1 (MQ=37)
                                                                                   gCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGGGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGc           >  1:6765574/1‑101 (MQ=25)
                                                                                   gCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGc           >  2:5103819/1‑101 (MQ=37)
                                                                                   gCCCCCCTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGc           >  1:2306351/1‑101 (MQ=37)
                                                                                     cccccTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCtt         <  2:3453993/101‑1 (MQ=37)
                                                                                     cccccTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCtt         <  1:2657020/101‑1 (MQ=37)
                                                                                      ccccTC.TTTTG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCTTg        <  1:1126210/101‑1 (MQ=37)
                                                                                         cctcttttG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCTTGCg      <  1:7056204/97‑1 (MQ=37)
                                                                                         cctcttttG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCTTGCg      <  1:731627/97‑1 (MQ=37)
                                                                                         cctcttttG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCTTGCg      <  1:3877349/97‑1 (MQ=37)
                                                                                          ctcttttG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCTTGCgg     <  1:2237895/98‑1 (MQ=38)
                                                                                          ctcttttG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCTTGCgg     <  1:3430453/98‑1 (MQ=38)
                                                                                          ctcttttG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCTTGCgg     <  2:3856962/98‑1 (MQ=38)
                                                                                          ctcttttG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCTTGCgg     >  1:10255/4‑101 (MQ=37)
                                                                                           tcttttG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCGCCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCTTGCGGa    <  2:1586119/99‑1 (MQ=37)
                                                                                            cttttG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCTTGCGGAc   <  1:7005944/100‑1 (MQ=255)
                                                                                            cttttG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCTTGCGGAc   <  2:9056122/100‑1 (MQ=255)
                                                                                             ttttG.GCTGCGC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCTTGCGGACg  <  1:1318594/101‑1 (MQ=255)
                                                                                                      ||                                                                                             
GGGCTGAGCGTGCTGCGCTGGCCGGTGCGGCGGCTCTTTGCCGCGATGCACCGGCGCGACTGAGGGCGGGGCAGGGGGGCGCTGCCCCCCTCTTTTTG.GCT..GC..CAAAATTCACCCCCCGGCGTATTTGCACCAGGAAAAAGCCCGGCGTTTTTCCTGGTCGAAATACGCCGGGGGGAGTCCGCTTGCGGACG  >  NC_014034/904834‑905025

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG