Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,734,8911.→A 2.3% 31.5 ‑5.6 ‑0.0478intergenic (‑144/+49)RCAP_rcc01603/RCAP_rcc01604hemolysin‑III family protein/hypothetical protein
Rejected: Prediction has biased read strand distribution supporting polymorphism.
Reads supporting (aligned to +/- strand):  new base (10/1):  ref base (212/255):  total (222/256)
Fisher's exact test strand distribution p-value = 3.64e-03
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.78e-01

Only 100 of 504 total aligned reads displayed.

GCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCA.GGGGGGCGCTG.CCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGC  >  NC_014034/1734793‑1734991
                                                                                                     |                                                                                                     
gCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.g                                                                                                      <  2:4812099/101‑1 (MQ=255)
  cAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGc                                                                                                    >  2:6911520/1‑101 (MQ=255)
    aaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCt                                                                                                  >  2:9079952/1‑101 (MQ=255)
    aaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCt                                                                                                  >  1:9110435/1‑101 (MQ=255)
     aGGCTGACGCTGCGTCAGCCGACGGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCtt                                                                                                 <  2:8094371/101‑1 (MQ=255)
     aGGCTGACGCTGCGTCAGCCGACGGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCtt                                                                                                 <  2:2026098/101‑1 (MQ=255)
     aGGCTGACGCTGCGTCAGCCGACGGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCtt                                                                                                 <  2:6664351/101‑1 (MQ=255)
     aGGCTGACGCTGCGTCAGCCGACGGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCtt                                                                                                 <  2:3711499/101‑1 (MQ=255)
      ggcgttctcggCGTCAGCCGACGGGCCGGGCA.GGGGCGCGCTGCCCCCCGTCCTTCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTc                                                                                                <  2:4289600/91‑1 (MQ=17)
      ggCTGCCGCTGCGTCAGGCGACGGGCCGGGAA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTc                                                                                                <  1:3762682/101‑1 (MQ=18)
      ggCTGACGCTGCGTCAGCCGACGGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTc                                                                                                <  1:4603959/101‑1 (MQ=255)
      ggCTGACGCTGCGTCAGCCGACGGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTc                                                                                                <  1:343646/101‑1 (MQ=255)
      ggCTGACGCTGCGTCAGCCGACGGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTc                                                                                                <  2:8725858/101‑1 (MQ=255)
        cTGACGCTGCGTCAGCCGACGGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCAt                                                                                              <  2:3859383/101‑1 (MQ=255)
        cTGACGCTGCGTCAGCCGACGGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCAt                                                                                              <  2:642340/101‑1 (MQ=255)
        cTGACGCTGCGTCAGCCGACGGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCAt                                                                                              <  2:2423944/101‑1 (MQ=255)
        cTGACGCTGCGTCAGCCGACGGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCAt                                                                                              >  2:176772/1‑101 (MQ=255)
         tGACGCTGCGTCAGCCGACGGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATc                                                                                             >  1:5183745/1‑101 (MQ=255)
         tGACGCTGCGTCAGCCGACGGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATc                                                                                             >  1:3708572/1‑101 (MQ=255)
          gACGCTGCGTCAGCCGACGGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGCTCTTTATGGCAAGATGGAACTGC.GGCCTTCTTCt                                                                                            >  1:4448404/1‑101 (MQ=18)
          gACGCTGCGTCAGCCGACGGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCt                                                                                            >  2:4080916/1‑101 (MQ=255)
           aCGCTGCGTCAGCCGGCGGGCCGGGCA.TGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCtt                                                                                           <  1:1613384/101‑1 (MQ=18)
           aCGCTGCGTCAGCCGACGGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCtt                                                                                           <  2:5881893/101‑1 (MQ=255)
            cGCTGCGTCAGCCGACGGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTg                                                                                          <  2:6267198/101‑1 (MQ=255)
             gCTGCGTCAGCCGACGGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGc                                                                                         <  2:5292699/101‑1 (MQ=255)
             gCTGCGTCAGCCGACGGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGc                                                                                         <  2:5614227/101‑1 (MQ=255)
               tGCGTCAGCCGACGGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCa                                                                                       >  2:2833852/1‑101 (MQ=255)
                 cGTCAGCCGACGGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATa                                                                                     <  1:1213689/101‑1 (MQ=255)
                  gTCAGCCGACGGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaa                                                                                    <  2:111088/101‑1 (MQ=255)
                  gTCAGCCGACGGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaa                                                                                    <  1:7712209/101‑1 (MQ=255)
                   tCAGCCGACGGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaaa                                                                                   >  1:2456467/1‑101 (MQ=255)
                    cAGCCGACGGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                                  <  2:6169763/101‑1 (MQ=255)
                     aGCCGACGGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAata                                                                                 <  2:7797060/101‑1 (MQ=255)
                     aGCCGACGGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAata                                                                                 <  2:1598293/101‑1 (MQ=255)
                      gCCGACGGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAatat                                                                                >  1:3320149/1‑101 (MQ=255)
                      gCCGACGGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAatat                                                                                <  1:5361354/101‑1 (MQ=255)
                          aagggccggggAGGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATccc                                                                             <  2:6042826/99‑1 (MQ=14)
                          aCGGGCCGGGCA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.g                                                                           <  2:3264977/101‑1 (MQ=255)
                            gggccgggcA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.ggg                                                                         <  1:2899663/101‑1 (MQ=255)
                            gggccgggcA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.ggg                                                                         >  2:3328649/1‑101 (MQ=255)
                            gggccgggcA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.ggg                                                                         >  2:8551026/1‑101 (MQ=255)
                               tgacggacggggggCTCTG.CCCCCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTg                                                                      >  2:1303256/9‑101 (MQ=2)
                               tgacggacggggggCTCTG.CCCCCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTg                                                                      >  1:3238446/9‑101 (MQ=2)
                                gacggacggggggCTCTG.CCCCCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTga                                                                     >  1:6930624/8‑100 (MQ=2)
                                 gggcA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCg                                                                    <  1:6091390/101‑1 (MQ=255)
                                 gggcA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCg                                                                    >  1:6818258/1‑101 (MQ=255)
                                  ggcA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCAATAAATATCCC.GGGGTGCgg                                                                   >  1:7201424/1‑101 (MQ=255)
                                   gcA.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCggg                                                                  <  1:5781132/101‑1 (MQ=255)
                                     a.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGc                                                                >  2:7582280/1‑101 (MQ=255)
                                     a.GGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGc                                                                <  1:6476344/101‑1 (MQ=255)
                                        gggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCag                                                              >  2:2955981/1‑101 (MQ=255)
                                          gggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGAGTATGTCTGGCACGATGAAACTGC.GCCCTTCATATTGCCATAAATATCCC.GGGATGCGAGGCagag                                                            >  2:2310273/1‑101 (MQ=14)
                                           ggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGc                                                           >  1:3882681/1‑101 (MQ=255)
                                            gcgcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGcc                                                          <  2:7162885/101‑1 (MQ=255)
                                             cgcctccccccGTCCTGCGGACCCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGccc                                                         <  2:2989945/95‑1 (MQ=255)
                                              gctgccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGcccc                                                        <  2:8897891/96‑1 (MQ=255)
                                                  ccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTc                                                    <  1:7113804/100‑1 (MQ=255)
                                                  ccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTc                                                    <  1:2987269/100‑1 (MQ=255)
                                                        gTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGGCATAAATATCCC.GGGCTGTGGGGCAGAGGACCGCTCAACCGa                                              >  2:2740338/1‑101 (MQ=21)
                                                          ccTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAg                                            >  2:3486325/1‑101 (MQ=255)
                                                            tGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGAt                                          >  1:8044753/1‑101 (MQ=255)
                                                                gACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACa                                      >  1:6651025/1‑101 (MQ=255)
                                                                gACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACa                                      <  1:1988648/101‑1 (MQ=255)
                                                                gACTCCCCACGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACa                                      <  1:8674283/101‑1 (MQ=255)
                                                                     cccccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATc                                 <  1:4259729/101‑1 (MQ=255)
                                                                     cccccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATc                                 <  2:2530322/101‑1 (MQ=255)
                                                                      ccccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATcc                                <  2:4696287/101‑1 (MQ=255)
                                                                      ccccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATcc                                <  2:5195943/101‑1 (MQ=255)
                                                                        ccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcgc                              >  1:7387120/1‑101 (MQ=255)
                                                                         cGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcgcg                             >  1:4188548/1‑101 (MQ=255)
                                                                         cGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcgcg                             >  2:3666805/1‑101 (MQ=255)
                                                                         cGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcgcg                             >  1:104331/1‑101 (MQ=255)
                                                                          gggATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCgg                            >  1:488443/1‑101 (MQ=255)
                                                                          gggATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCgg                            >  1:1815322/1‑101 (MQ=255)
                                                                            gATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGcc                          <  1:3054076/101‑1 (MQ=255)
                                                                            gATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGcc                          >  2:5823952/1‑101 (MQ=255)
                                                                             atatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGccc                         >  1:1383612/1‑101 (MQ=255)
                                                                              tatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCg                        >  1:5074066/1‑101 (MQ=255)
                                                                              tatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCg                        >  2:8447062/1‑101 (MQ=255)
                                                                              tatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCg                        >  1:8589309/1‑101 (MQ=255)
                                                                              tatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCg                        <  1:8351456/101‑1 (MQ=255)
                                                                                tttATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGt                      >  1:4086414/1‑101 (MQ=255)
                                                                                tttATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGt                      >  1:7500165/1‑101 (MQ=255)
                                                                                tttATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGt                      <  2:2223069/101‑1 (MQ=255)
                                                                                 ttATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCCGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCCGTCCCGtt                     >  2:7480266/1‑101 (MQ=37)
                                                                                  tATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGttt                    >  1:4517702/1‑101 (MQ=255)
                                                                                   aTGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGtttt                   <  2:4576120/101‑1 (MQ=255)
                                                                                   aTGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGtttt                   >  2:5374668/1‑101 (MQ=255)
                                                                                   aTGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC..GGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCCCGGCCCGGTTTTc                  >  2:496030/1‑101 (MQ=255)
                                                                                     ggCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCg                 >  1:2143028/1‑101 (MQ=255)
                                                                                        aaGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtg              >  2:3156447/1‑101 (MQ=255)
                                                                                          gATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgat            >  1:5546298/1‑101 (MQ=255)
                                                                                          gATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgat            <  1:6222904/101‑1 (MQ=255)
                                                                                          gATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGAtgag            >  2:3980193/1‑100 (MQ=255)
                                                                                           aTGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgatg           <  1:2721451/101‑1 (MQ=255)
                                                                                             gAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAg         <  2:143622/101‑1 (MQ=255)
                                                                                               aaCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCa       >  1:2254797/1‑101 (MQ=255)
                                                                                               aaCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCa       <  2:1939824/101‑1 (MQ=255)
                                                                                                 cTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAg     >  1:8993650/1‑101 (MQ=255)
                                                                                                   gC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAgcg   >  1:5999694/1‑101 (MQ=255)
                                                                                                     |                                                                                                     
GCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCA.GGGGGGCGCTG.CCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGC  >  NC_014034/1734793‑1734991

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG