Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,255,867 T→C 100% intergenic (+37/+205) RCAP_rcc01184 → / ← RCAP_rcc01185 CsbD family protein/methyl‑accepting chemotaxis sensory transducer

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,255,8670T→C100.0% 330.9 155intergenic (+37/+205)RCAP_rcc01184/RCAP_rcc01185CsbD family protein/methyl‑accepting chemotaxis sensory transducer
Reads supporting (aligned to +/- strand):  new base (90/65):  ref base (0/0):  total (90/65)

Only 100 of 158 total aligned reads displayed.

AAGAAGGAAGTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGG....CTG.TCT.CGGTCCGCCCGAAGAGGGGGGGCGCTGTGGTAC..CCCTGCGGGACTCCCCCCGGGATATTTCCGCCAA  >  NC_014034/1255795‑1255938
                                                                            |                                                                           
aagaagGAAGTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAgggg                                                    >  2:2719263/1‑101 (MQ=255)
aagaagGAAGTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAgggg                                                    >  1:8172712/1‑101 (MQ=255)
aagaagGAAGTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAgggg                                                    >  2:634591/1‑101 (MQ=255)
 agaagGAAGTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAggggg                                                   >  2:3968703/1‑101 (MQ=255)
 agaagGAAGTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAggggg                                                   >  1:2067910/1‑101 (MQ=255)
  gaaggaagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAggggcg                                                  >  2:68509/1‑99 (MQ=255)
   aaggaagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAggggggg                                                 >  2:3298425/1‑101 (MQ=255)
   aaggaagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAggggggg                                                 >  1:2687843/1‑101 (MQ=255)
   aaggaagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAggggggg                                                 >  1:2472299/1‑101 (MQ=255)
   aaggaagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAggggggg                                                 <  1:867396/101‑1 (MQ=255)
   aaggaagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAggggggg                                                 >  2:2390383/1‑101 (MQ=255)
    aggaagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGgc                                                >  1:2612854/1‑101 (MQ=255)
    aggaagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGgc                                                <  1:437071/101‑1 (MQ=255)
     ggaagTCGACGACTTCCTGTGGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGCgcg                                               <  1:7221570/101‑1 (MQ=255)
     ggaagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGgcg                                               <  1:5311798/101‑1 (MQ=255)
     ggaagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGgcg                                               >  1:8678237/1‑101 (MQ=255)
      gaagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGgcgc                                              >  2:60851/1‑101 (MQ=255)
      gaagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGgcgc                                              >  1:277176/1‑101 (MQ=255)
       aagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGCAGAGGGGGGGCGCt                                             >  2:6431635/1‑101 (MQ=255)
       aagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCt                                             <  1:407193/101‑1 (MQ=255)
       aagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCt                                             <  2:3732488/101‑1 (MQ=255)
       aagTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCt                                             <  2:6527826/101‑1 (MQ=255)
        agTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGgcgccg                                            >  1:6534541/1‑99 (MQ=255)
        agTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCtg                                            >  1:8886240/1‑101 (MQ=255)
        agTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCtg                                            <  2:311670/101‑1 (MQ=255)
        agTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCtg                                            >  1:2433856/1‑101 (MQ=255)
          tCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCtgcc                                          <  2:1976674/101‑3 (MQ=255)
           cgacgaCTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCtgccc                                         <  2:8378296/101‑4 (MQ=255)
           cgacgaCTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCtgccc                                         <  1:2390456/101‑4 (MQ=255)
            gacgacTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCtgcccc                                        >  1:2880184/1‑97 (MQ=255)
            gacgacTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCtgcccc                                        >  1:1190155/1‑97 (MQ=255)
             acgacTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCtgccccc                                       <  1:916005/101‑6 (MQ=255)
             acgacTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCtgccccc                                       >  1:7276948/1‑96 (MQ=255)
              cgacTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCtgcccccc                                      <  1:5198332/101‑7 (MQ=255)
              cgacTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCtgcccccc                                      <  1:3106758/101‑7 (MQ=255)
              cgacTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCtgcccccc                                      <  2:128685/101‑7 (MQ=255)
              cgacTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCtgcccccc                                      <  2:7769951/101‑7 (MQ=255)
              cgacTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCtgcccccc                                      <  1:1874246/101‑7 (MQ=255)
              cgacTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCtgcccccc                                      <  2:4244384/101‑7 (MQ=255)
               gacTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCtgccccccg                                     >  1:7835431/1‑94 (MQ=255)
               gacTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCtgccccccc                                     >  1:4219/1‑94 (MQ=255)
                acTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCtgccccccgt                                    <  2:1041000/101‑9 (MQ=255)
                  ttCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCtgccccccgtcc                                  <  1:4338399/101‑11 (MQ=255)
                  ttCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCtgccccccgtcc                                  >  2:7408636/1‑91 (MQ=255)
                  ttCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCtgccccccgtcc                                  <  2:5797493/101‑11 (MQ=255)
                  ttCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCtgccccccgtcc                                  >  2:4199642/1‑91 (MQ=255)
                                 tGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTccccc                   >  1:758075/1‑101 (MQ=9)
                                 tGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTccccc                   >  1:6157607/1‑101 (MQ=9)
                                 tGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTccccc                   >  1:6119735/1‑101 (MQ=9)
                                  ggccTGGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTcccccc                  <  2:3362361/99‑1 (MQ=2)
                                  gcccgaGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTcccccc                  <  2:8940327/96‑1 (MQ=2)
                                  gACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTcccccc                  <  2:5280321/101‑1 (MQ=9)
                                  gACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTcccccc                  >  1:547328/1‑101 (MQ=9)
                                  gACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTcccccc                  >  1:5433929/1‑101 (MQ=9)
                                   tacTAGGGGTGCCGGACCGGAGCCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCg                 <  2:636453/99‑1 (MQ=2)
                                   aCCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCg                 <  2:3972107/101‑1 (MQ=9)
                                   aCCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCg                 >  1:70064/1‑101 (MQ=9)
                                   aCCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCg                 >  2:7056786/1‑101 (MQ=9)
                                   aCCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCg                 <  1:2348612/101‑1 (MQ=9)
                                    ccTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCgg                >  2:8353366/1‑101 (MQ=2)
                                     cTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCggg               >  2:781545/1‑101 (MQ=2)
                                     cTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCggg               <  2:2472226/101‑1 (MQ=2)
                                     cTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCggg               >  1:1154146/1‑101 (MQ=2)
                                     cTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCggg               >  2:1960484/1‑101 (MQ=2)
                                     cTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCggg               >  2:1492546/1‑101 (MQ=2)
                                     cTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCggg               >  1:6456892/1‑101 (MQ=2)
                                      tAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGa              >  1:1336300/1‑101 (MQ=2)
                                      tAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGa              >  2:8155869/1‑101 (MQ=2)
                                      tAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGa              >  1:3162362/1‑101 (MQ=2)
                                      tAGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGa              >  1:9118625/1‑101 (MQ=2)
                                       aGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGat             <  1:1790427/101‑1 (MQ=2)
                                       aGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGat             >  1:4570310/1‑101 (MQ=2)
                                       aGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGat             <  1:7557706/101‑1 (MQ=2)
                                       aGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGat             <  2:2109370/101‑1 (MQ=2)
                                       aGGGGTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGat             >  1:9144580/1‑101 (MQ=2)
                                        ggggTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGata            <  2:8001583/101‑1 (MQ=2)
                                        ggggTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGata            >  2:1291988/1‑101 (MQ=2)
                                        ggggTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGata            <  2:8586238/101‑1 (MQ=2)
                                        ggggTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGata            <  2:5123517/101‑1 (MQ=2)
                                         gggTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGatat           >  2:5617128/1‑101 (MQ=2)
                                         gggTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGatat           <  1:3730415/101‑1 (MQ=2)
                                         gggTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGatat           >  2:4543226/1‑101 (MQ=2)
                                          ggggCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATAtt          <  2:5402703/98‑1 (MQ=0)
                                          ggTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATAtt          <  1:4826479/101‑1 (MQ=2)
                                          ggTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATAtt          >  2:3113227/1‑101 (MQ=2)
                                          ggTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATAtt          <  1:835944/101‑1 (MQ=2)
                                          ggTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATAtt          <  2:4895539/101‑1 (MQ=2)
                                          ggTCCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATAtt          <  1:5211890/101‑1 (MQ=0)
                                           gTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATAttt         >  1:3146065/1‑101 (MQ=0)
                                           gTGCCGGACCGGAACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCATGGCTAttt         >  2:8168488/1‑101 (MQ=0)
                                               cggaccggaACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGc     >  1:1329147/1‑101 (MQ=2)
                                               cggaccggaACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGc     >  2:2049236/1‑101 (MQ=2)
                                                ggaccggaACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGcc    <  2:4689695/101‑1 (MQ=2)
                                                 gaccggaACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCa   <  1:5320169/101‑1 (MQ=2)
                                                 gaccggaACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCa   <  1:8256188/101‑1 (MQ=2)
                                                 gaccggaACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCa   <  1:7670399/101‑1 (MQ=2)
                                                 gaccggaACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCa   >  2:4320609/1‑101 (MQ=2)
                                                 gaccggaACCGGGGGCCTTCGGGCCCCCG.CTTCCGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCa   <  1:4676616/101‑1 (MQ=2)
                                                  accggaACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCaa  >  1:6721126/1‑101 (MQ=2)
                                                  accggaACCGGGGGCCTTCGGGCCCCCGCTTT.CGGTCCGCCCGAAGAGGGGGGGCGCTGCCCCCCGTCCCTGCGGGACTCCCCCCGGGATATTTCCGCCaa  <  2:8625970/101‑1 (MQ=2)
                                                                            |                                                                           
AAGAAGGAAGTCGACGACTTCCTGTCGAAACTCTGACCTAGGGGTGCCGGACCGGAACCGGGGGCCTTCGG....CTG.TCT.CGGTCCGCCCGAAGAGGGGGGGCGCTGTGGTAC..CCCTGCGGGACTCCCCCCGGGATATTTCCGCCAA  >  NC_014034/1255795‑1255938

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG