Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,283,564 A→G 100% T305A (ACG→GCG)  ppdK → pyruvate, phosphate dikinase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,283,5640A→G100.0% 1163.6 319T305A (ACG→GCG) ppdKpyruvate, phosphate dikinase
Reads supporting (aligned to +/- strand):  new base (162/157):  ref base (0/0):  total (162/157)

Only 100 of 336 total aligned reads displayed.

CGGTGCTCTGCGGCTATGGCTCTGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACACGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGATCATCACCCGCGAAGATGCGCTCTTGCGGGT  >  NC_014034/1283466‑1283661
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cgGTGCTCTGCGGCTATGGCTCTGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGGGATCGACGCg                                                                                                 >  1:7706946/1‑101 (MQ=255)
 ggTGCTCTGCGGCTATGGCTCTGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCgg                                                                                                >  2:6819543/1‑101 (MQ=255)
  gTGCTCTGCGGCTATGGCTCTGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCggg                                                                                               >  1:1121992/1‑100 (MQ=255)
   tGCTCTGCGGCTATGGCTCTGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTg                                                                                              <  2:6324877/101‑1 (MQ=255)
   tGCTCTGCGGCTATGGCTCTGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTg                                                                                              <  1:2112492/101‑1 (MQ=255)
   tGCTCTGCGGCTATGGCTCTGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTg                                                                                              <  1:2478530/101‑1 (MQ=255)
    gCTCTGCGGCTATGGCTCTGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGc                                                                                             >  1:4947551/1‑101 (MQ=255)
     ctctGCGGCTATGGCTCTGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCg                                                                                            >  1:8575335/1‑101 (MQ=255)
       cgcgggCTAGGGCTCTGCGGGGCGGGCGCGGCGGCGCGAGGAAAGGCAGCTGGAATTCGCGATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCggg                                                                                          <  2:2976612/97‑1 (MQ=255)
        tGCGGCTATGGCTCTGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGt                                                                                         <  1:4575933/101‑1 (MQ=255)
         gCGGCTATGGCTCTGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGGTTTCGGTGATCGACGCGGCGCgggag                                                                                        >  2:1516316/1‑99 (MQ=255)
         gCGGCTATGGCTCTGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTg                                                                                        >  2:5025605/1‑101 (MQ=255)
         gCGGCTATGGCTCTGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTg                                                                                        <  1:3117039/101‑1 (MQ=255)
          cGGCTATGGCTCTGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTgg                                                                                       >  1:7567102/1‑101 (MQ=255)
            gCTATGGCTCTGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGGGATCGACGCGGTGCGGGGGgcg                                                                                     >  1:7447584/1‑101 (MQ=255)
              tATGGCTCTGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGgcgcg                                                                                   >  1:4666318/1‑101 (MQ=255)
              tATGGCTCTGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGgcgcg                                                                                   >  1:2844994/1‑101 (MQ=255)
              tATGGCTCTGCCTGCCGGGAGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGCAGGCGGGGGGATTTCGGTGCTCGACGCGGTGCCGGTGgcgcc                                                                                   >  2:3412840/1‑100 (MQ=255)
               aTGGCTCTGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGGGgcgcgc                                                                                  >  1:1388170/1‑101 (MQ=255)
               aTGGCTCTGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGGGgcgcgc                                                                                  >  1:5365060/1‑101 (MQ=255)
                tGGCTCTGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGAGGGGGgcgcgcc                                                                                 >  2:2535749/1‑100 (MQ=255)
                 ggCTCTGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTc                                                                                <  1:1620513/101‑1 (MQ=255)
                  gCTCTGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGGGATCGACGCGGTGCGGGTGGCGCGCTCg                                                                               >  2:8205119/1‑101 (MQ=255)
                   ctctGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCgg                                                                              <  1:6162498/101‑1 (MQ=255)
                    tctGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGc                                                                             <  2:6393284/101‑1 (MQ=255)
                    tctGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGc                                                                             >  1:6031309/1‑101 (MQ=255)
                     ctgcctgcCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGcc                                                                            <  2:7734594/101‑1 (MQ=255)
                      tgcctgccGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGccc                                                                           <  2:18137/101‑1 (MQ=255)
                       gcctgccGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCg                                                                          <  1:4838651/101‑1 (MQ=255)
                          tgccGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGc                                                                       <  1:2968813/101‑1 (MQ=255)
                          tgccGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGc                                                                       >  1:8755590/1‑101 (MQ=255)
                            ccGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGa                                                                     >  1:5688019/1‑101 (MQ=255)
                            ccGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGa                                                                     <  2:8118438/101‑1 (MQ=255)
                             cGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGAc                                                                    <  2:5136079/101‑1 (MQ=255)
                                gcgcGGCTGCGCGAGGAAATGCAGCTCGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGt                                                                 <  2:6057276/101‑1 (MQ=255)
                                  gcgGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGa                                                               <  2:781920/101‑1 (MQ=255)
                                    ggCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAg                                                             <  2:756607/101‑1 (MQ=255)
                                     gCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGa                                                            >  2:3865439/1‑101 (MQ=255)
                                       tGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATc                                                          <  2:1906097/101‑1 (MQ=255)
                                       tGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATc                                                          >  1:8195325/1‑101 (MQ=255)
                                         cgcgAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATcgc                                                        >  1:5887013/1‑101 (MQ=255)
                                         cgcgAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATcgc                                                        <  2:5847541/101‑1 (MQ=255)
                                            gAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCggg                                                     >  1:5790382/1‑100 (MQ=255)
                                                aaaTGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGGGAAGATCGCGGTGGCt                                                 >  2:8841916/1‑101 (MQ=255)
                                                 aaTGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCtt                                                <  1:1840505/101‑1 (MQ=255)
                                                  aTGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCttt                                               <  2:4658993/101‑1 (MQ=255)
                                                  aTGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCttt                                               >  2:5750636/1‑101 (MQ=255)
                                                  aTGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCttt                                               >  2:1691515/1‑101 (MQ=255)
                                                  aTGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTAAAGATCGCGGTGGCttt                                               <  2:8574950/101‑1 (MQ=255)
                                                   tGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTg                                              <  2:5255267/101‑1 (MQ=255)
                                                    gCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTgg                                             >  2:4811116/1‑101 (MQ=255)
                                                    gCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTgg                                             <  2:3280906/101‑1 (MQ=255)
                                                       gCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCg                                          <  1:3283730/101‑1 (MQ=255)
                                                        gtGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGa                                         <  1:6753679/100‑1 (MQ=255)
                                                          ggAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAgg                                       <  2:6500476/101‑1 (MQ=255)
                                                          ggAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAgg                                       >  1:6681259/1‑101 (MQ=255)
                                                          ggAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAgg                                       <  2:132566/101‑1 (MQ=255)
                                                          ggAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTCGGCCGAgg                                       >  2:842920/1‑101 (MQ=255)
                                                            aaTTCGCCATTGAAGACGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCACGGTGGCTTTGGCCGAGGAc                                     >  1:7253971/1‑101 (MQ=255)
                                                               tCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACggg                                  >  2:6717184/1‑101 (MQ=255)
                                                               tCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACggg                                  <  1:6483803/101‑1 (MQ=255)
                                                               tCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACggg                                  >  1:4901839/1‑101 (MQ=255)
                                                               tCGCCAGTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACggg                                  <  2:4042636/101‑1 (MQ=255)
                                                                cGCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGa                                 >  2:6364189/1‑101 (MQ=255)
                                                                 gCCATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGat                                >  2:7142536/1‑101 (MQ=255)
                                                                  ccATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGatc                               <  1:2554053/101‑1 (MQ=255)
                                                                  ccATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGatc                               >  2:2208603/1‑101 (MQ=255)
                                                                   cATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGatca                              <  1:4313894/101‑1 (MQ=255)
                                                                   cATTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGatca                              >  2:5357235/1‑101 (MQ=255)
                                                                    aTTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGatcat                             >  1:5243549/1‑101 (MQ=255)
                                                                    aTTGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGatcat                             <  1:3402610/101‑1 (MQ=255)
                                                                     ttGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGatcatc                            >  1:4197783/1‑101 (MQ=255)
                                                                      tGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGAtcatca                           <  1:4061033/101‑1 (MQ=255)
                                                                      tGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGAtcatca                           <  2:2251201/101‑1 (MQ=255)
                                                                      tGAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGGGGGGCGCTCGGCCCGGGGGACGGTGAAGATGGGGGTGGCTTGGGCCGAGGACgggggcgtca                           >  1:789887/1‑94 (MQ=255)
                                                                       gAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGATCATCAc                          <  1:8054754/101‑1 (MQ=255)
                                                                       gAAGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGATCATCAc                          >  1:7123409/1‑101 (MQ=255)
                                                                       gAAGGCGGGCGGATTTCGGTGATCGACGCGGCGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGAGGGCTTTGGCCGAGGACGGCGTCATCAc                          >  2:7392087/1‑101 (MQ=255)
                                                                         aGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGATCATCAccc                        <  2:2697780/101‑1 (MQ=255)
                                                                         aGGCGGGCGGATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGATAATCAccc                        >  2:2786971/1‑101 (MQ=255)
                                                                             gggcggATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGATCATCACCCGCGa                    >  1:5167212/1‑101 (MQ=255)
                                                                              ggcggATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACNGGATCATCACCCGCGaa                   >  2:2106531/1‑101 (MQ=255)
                                                                               gcggATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGGCGGGATCATCACCCGCGAAg                  >  2:1139240/1‑101 (MQ=255)
                                                                               gcggATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGATCATCACCCGCGAAg                  <  1:784352/101‑1 (MQ=255)
                                                                               gcggATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGATCATCACCCGCGAAg                  <  2:2405548/101‑1 (MQ=255)
                                                                                cggATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGATCATCACCCGCGAAGa                 <  2:3518181/101‑1 (MQ=255)
                                                                                 ggATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGATCATCACCCGCGAAGAt                >  2:2557030/1‑101 (MQ=255)
                                                                                 ggATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGATCATCACCCGCGAAGAt                >  1:9106839/1‑101 (MQ=255)
                                                                                  gATTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCAAGGACGGGATCATCACCCGCGAAGATg               >  1:2083206/1‑101 (MQ=255)
                                                                                   aTTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGATCATCACCCGCGAAGATgc              <  2:7959958/101‑1 (MQ=255)
                                                                                   aTTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGATCATCACCCGCGAAGATgc              <  2:1414378/101‑1 (MQ=255)
                                                                                   aTTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGATCATCACCCGCGAAGATgc              <  1:655126/101‑1 (MQ=255)
                                                                                   aTTTCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGATCATCACCCGCGAAGATgc              >  2:6217157/1‑101 (MQ=255)
                                                                                     ttCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGATCATCACCCGCGAAGATgcgc            >  1:2349761/1‑101 (MQ=255)
                                                                                      tCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGATCATCACCCGCGAAGATGCGct           <  2:3658283/101‑1 (MQ=255)
                                                                                      tCGGTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGATCATCACCCGCGAAGATGCGct           <  2:4426864/101‑1 (MQ=255)
                                                                                        ggTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGTCTTTGGCCGAGGACGGGATCATCACCCGCGAAGATGCGctct         <  2:2945287/101‑1 (MQ=255)
                                                                                         gTGATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGATCATCACCCGCGAAGATGCGCTCtt        <  1:8364687/101‑1 (MQ=255)
                                                                                           ggtCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGATCATCACCCGCGAAGATGCGCTCTTGc      >  1:5484530/3‑101 (MQ=255)
                                                                                           gATCGACGCGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGATCATCACCCGCGAAGATGCGCTCTTGc      <  1:3976585/101‑1 (MQ=255)
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CGGTGCTCTGCGGCTATGGCTCTGCCTGCCGGGCGCGGCTGCGCGAGGAAATGCAGCTGGAATTCGCCATTGAAGGCGGGCGGATTTCGGTGATCGACACGGTGCGGGTGGCGCGCTCGGCCCGGGCGACGGTGAAGATCGCGGTGGCTTTGGCCGAGGACGGGATCATCACCCGCGAAGATGCGCTCTTGCGGGT  >  NC_014034/1283466‑1283661

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG