Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,342,925 G→T 0.8% D421Y (GAC→TAC)  RCAP_rcc01264 → reverse transcriptase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,342,9250G→T 0.8% 2.6 ‑4.4 ‑0.0623D421Y (GAC→TAC) RCAP_rcc01264reverse transcriptase
Reads supporting (aligned to +/- strand):  new base (0/6):  ref base (323/294):  total (323/300)
Fisher's exact test strand distribution p-value = 1.21e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 642 total aligned reads displayed.

CATCCCCAATTTTGACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAGAATGACG  >  NC_014034/1342825‑1343025
                                                                                                    |                                                                                                    
 aTCCCCAATTTTGACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGa                                                                                                     >  2:352642/1‑101 (MQ=255)
   ccccAATTTTGACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACg                                                                                                   <  1:3424866/101‑1 (MQ=255)
     ccAATTTTGACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGaa                                                                                                 <  1:4180267/101‑1 (MQ=255)
       aaTTTTGACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAAt                                                                                               <  1:2604073/101‑1 (MQ=255)
        aTTTTGACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATa                                                                                              <  1:8084954/101‑1 (MQ=255)
        aTTTTGACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATa                                                                                              <  1:4679778/101‑1 (MQ=255)
        aTTTTGACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATa                                                                                              <  2:5142898/101‑1 (MQ=255)
        aTTTTGACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATa                                                                                              >  2:5383977/1‑101 (MQ=255)
         ttttGACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATaa                                                                                             <  1:8390594/101‑1 (MQ=255)
             gACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACCTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGc                                                                                         >  2:6848935/1‑101 (MQ=255)
               cAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTg                                                                                       >  1:3340755/1‑101 (MQ=255)
                aaaTATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGc                                                                                      <  1:7509265/101‑1 (MQ=255)
                aaaTATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGc                                                                                      >  2:6324174/1‑101 (MQ=255)
                aaaTATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAAAGCTga                                                                                      >  1:5746085/1‑100 (MQ=255)
                  atatGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCAt                                                                                    >  1:4322371/1‑101 (MQ=255)
                  atatGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCAt                                                                                    >  2:2006910/1‑101 (MQ=255)
                  atatGGGCTTGAGGAGAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCAt                                                                                    >  1:8844493/1‑101 (MQ=255)
                   tatGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCAtt                                                                                   >  1:6718799/1‑101 (MQ=255)
                    atGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTc                                                                                  <  2:5941435/101‑1 (MQ=255)
                    atGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTc                                                                                  <  1:1829388/101‑1 (MQ=255)
                    atGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTc                                                                                  >  2:887660/1‑101 (MQ=255)
                     tGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTcc                                                                                 <  1:2349243/101‑1 (MQ=255)
                     tGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTcc                                                                                 <  2:3209083/101‑1 (MQ=255)
                       ggCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGa                                                                               <  1:7026900/101‑1 (MQ=255)
                       ggCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGa                                                                               >  2:6139304/1‑101 (MQ=255)
                        gCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGaa                                                                              >  1:2964089/1‑101 (MQ=255)
                        gCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGaa                                                                              <  1:2762409/101‑1 (MQ=255)
                          ttGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACt                                                                            <  2:4773470/101‑1 (MQ=255)
                          ttGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACt                                                                            >  2:6541446/1‑101 (MQ=255)
                           tGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACtt                                                                           >  1:6238433/1‑101 (MQ=255)
                           tGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACtt                                                                           >  1:8214534/1‑101 (MQ=255)
                           tGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACtt                                                                           >  2:3666789/1‑101 (MQ=255)
                           tGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACtt                                                                           >  1:6860196/1‑101 (MQ=255)
                            gAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTg                                                                          <  1:4394642/101‑1 (MQ=255)
                             aGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGc                                                                         <  2:8228803/101‑1 (MQ=255)
                               gATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCAt                                                                       <  2:6744335/101‑1 (MQ=255)
                                aTAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCAtt                                                                      >  2:1121917/1‑101 (MQ=255)
                                aTAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCAtt                                                                      <  1:3839006/101‑1 (MQ=255)
                                aTAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCAtt                                                                      <  2:5052936/101‑1 (MQ=255)
                                aTAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCAtt                                                                      <  1:3198461/101‑1 (MQ=255)
                                 tAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTa                                                                     >  2:909834/1‑101 (MQ=255)
                                  aaGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTaa                                                                    >  1:5958260/1‑101 (MQ=255)
                                   agagGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAAc                                                                   >  2:8663802/1‑101 (MQ=255)
                                   agagGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAAc                                                                   >  1:1954079/1‑101 (MQ=255)
                                    gagGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACCAAATAAACGCTGCATTCCGAACTTGCATTAACa                                                                  <  1:4780252/101‑1 (MQ=255)
                                     agGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACaa                                                                 <  2:3854679/101‑1 (MQ=255)
                                      ggCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGGCGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAg                                                                <  2:1789156/101‑1 (MQ=255)
                                      ggCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAg                                                                >  2:7890200/1‑101 (MQ=255)
                                        cGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGa                                                              >  1:1530545/1‑101 (MQ=255)
                                         gagaTATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGaa                                                             >  1:1116223/1‑101 (MQ=255)
                                           gaTATTTGTCTGCTGGCCCCGCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGCAAt                                                           <  1:7201542/101‑1 (MQ=255)
                                           gaTATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAAt                                                           <  1:1424767/101‑1 (MQ=255)
                                              atTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAAtctc                                                        >  1:2237625/1‑101 (MQ=255)
                                              atTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCGCGATTCCGAACTTGCATTAACAAGGAAAtctc                                                        >  2:4216592/1‑101 (MQ=255)
                                               tttGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCg                                                       >  1:7770222/1‑101 (MQ=255)
                                                  gTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGat                                                    <  1:6435666/101‑1 (MQ=255)
                                                  gTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGat                                                    <  2:3977208/101‑1 (MQ=255)
                                                    ctgctgGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGatat                                                  <  1:6787291/101‑1 (MQ=255)
                                                    ctgctgGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGatat                                                  <  1:6059078/101‑1 (MQ=255)
                                                    ctgctgGCCCCCCACTTATTTTCTGCTCGTGTCGAACGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGatat                                                  <  1:6482763/101‑1 (MQ=255)
                                                     tgctgGCCCCCCACTTATTTTTTGCTCGTGTTGAAAGCATGACCGCGGACGGAATAAAAGCTGCATTTCGAACCTGCATTAACAAGGAAATCTCGGatatg                                                 >  2:439202/1‑100 (MQ=255)
                                                        tgGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCgag                                              >  2:2815819/1‑101 (MQ=255)
                                                         ggCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCgaga                                             <  1:2701260/101‑1 (MQ=255)
                                                         ggCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCgaga                                             >  2:1913384/1‑101 (MQ=255)
                                                         ggCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCgaga                                             >  2:2668252/1‑101 (MQ=255)
                                                           ccccccACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGaaa                                           <  1:2477866/101‑1 (MQ=255)
                                                                 aCTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTg                                     <  1:1010087/101‑1 (MQ=255)
                                                                   ttATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCt                                   >  2:7190575/1‑101 (MQ=255)
                                                                   ttATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCt                                   >  2:6640922/1‑101 (MQ=255)
                                                                   ttATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCt                                   <  1:7605580/101‑1 (MQ=255)
                                                                    tATTTTCTGCTTTTTTTGAAAAGAAGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCtt                                  <  1:2042701/101‑1 (MQ=255)
                                                                     aTTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGCAAGACCTGCTTg                                 >  2:2301215/1‑101 (MQ=255)
                                                                     aTTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTg                                 >  2:291374/1‑101 (MQ=255)
                                                                       tttCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAg                               <  1:8965080/101‑1 (MQ=255)
                                                                        ttCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAgg                              >  1:5043546/1‑101 (MQ=255)
                                                                         tCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGc                             <  1:9560/101‑1 (MQ=255)
                                                                            gCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGATGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATAGAGAAAGACCTGCTTGAGGCAtt                          >  1:9087766/1‑101 (MQ=255)
                                                                               cGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTc                       <  1:5127917/101‑1 (MQ=255)
                                                                                 tgtTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGt                     >  2:7609743/1‑101 (MQ=255)
                                                                                 tgtTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGt                     >  1:3736885/1‑101 (MQ=255)
                                                                                 tgtTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGt                     >  2:4500956/1‑101 (MQ=255)
                                                                                 tgtTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAAGCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGt                     >  1:6573528/1‑101 (MQ=255)
                                                                                    tGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAAt                  <  2:493183/101‑1 (MQ=255)
                                                                                     gAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATg                 >  1:3551040/1‑101 (MQ=255)
                                                                                     gAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATg                 >  2:7468558/1‑101 (MQ=255)
                                                                                      aaaGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGt                >  1:8685855/1‑101 (MQ=255)
                                                                                        aGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTc              >  2:1638690/1‑101 (MQ=255)
                                                                                        aGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTc              <  2:3340651/101‑1 (MQ=255)
                                                                                           aTGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGcc           <  2:7006801/101‑1 (MQ=255)
                                                                                            tGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAAACCTGCTTGAGGCATTTTCTTAATGTTCGCCa          >  2:1475626/1‑101 (MQ=255)
                                                                                             gACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAg         <  1:1702932/101‑1 (MQ=255)
                                                                                             gACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAg         <  1:5477090/101‑1 (MQ=255)
                                                                                             gACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAg         <  1:4463611/101‑1 (MQ=255)
                                                                                                ggCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAGAAt      <  2:2472489/101‑1 (MQ=255)
                                                                                                 gCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAGAATg     >  1:7965537/1‑101 (MQ=255)
                                                                                                 gCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAGAATg     >  2:5733191/1‑101 (MQ=255)
                                                                                                  cAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAGAATGa    >  2:8370940/1‑101 (MQ=255)
                                                                                                  cAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAGAATGa    >  2:8551119/1‑101 (MQ=255)
                                                                                                   aGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAGAATGAc   <  1:6324414/101‑1 (MQ=255)
                                                                                                   aGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAGAATGAc   >  1:526387/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
CATCCCCAATTTTGACAAATATGGGCTTGAGGATAAGAGGCGAGATATTTGTCTGCTGGCCCCCCACTTATTTTCTGCTCGTGTTGAAAGCATGACGGCAGACGAAATAAACGCTGCATTCCGAACTTGCATTAACAAGGAAATCTCGGATATCGAGAAAGACCTGCTTGAGGCATTTTCGTAATGTTCGCCAGAATGACG  >  NC_014034/1342825‑1343025

Base quality scores:  ATCG  < 3 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG