Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,412 T→C 3.8% intergenic (+224/‑707) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,4120T→C 3.8% 28.3 ‑0.4 ‑0.2477intergenic (+224/‑707)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (10/9):  ref base (214/244):  total (224/253)
Fisher's exact test strand distribution p-value = 6.46e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.00e-01

Only 100 of 511 total aligned reads displayed.

TTCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCG  >  NC_014034/1395312‑1395512
                                                                                                    |                                                                                                      
ttCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCt                                                                                                        <  2:2505505/101‑1 (MQ=255)
  cGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGc                                                                                                      >  1:129209/1‑101 (MQ=255)
   gCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGTCCCTgtc                                                                                                     >  2:6482542/1‑99 (MQ=255)
   gCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGcc                                                                                                     <  1:5903012/101‑1 (MQ=255)
     accaccGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcgg                                                                                                   >  1:9016607/1‑101 (MQ=255)
       caccGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggtg                                                                                                 <  1:2654201/101‑1 (MQ=255)
        accGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggtga                                                                                                >  2:6363175/1‑101 (MQ=255)
        accGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggtga                                                                                                <  1:3735278/101‑1 (MQ=255)
         ccGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggtgat                                                                                               <  1:8001517/101‑1 (MQ=255)
               cAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggtgatcggtg.a                                                                                        >  2:1458549/1‑101 (MQ=255)
                 aTTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCggtgatcggtg.atc                                                                                      <  2:201637/101‑1 (MQ=255)
                  tttACCCCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCa                                                                                     <  2:7943756/101‑1 (MQ=255)
                  tttACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCa                                                                                     <  1:3222258/101‑1 (MQ=255)
                   ttACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAg                                                                                    >  1:8639735/1‑101 (MQ=255)
                    tACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGc                                                                                   >  1:1821164/1‑101 (MQ=255)
                     acacCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCg                                                                                  <  2:8725353/101‑1 (MQ=255)
                     acacCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCg                                                                                  >  2:3121670/1‑101 (MQ=255)
                     acacCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCg                                                                                  >  1:3637000/1‑101 (MQ=255)
                       acCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGt                                                                                <  2:8639349/101‑1 (MQ=255)
                       acCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGt                                                                                <  1:194578/101‑1 (MQ=255)
                       acCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGt                                                                                <  2:6863594/101‑1 (MQ=255)
                        ccGTCTGGTCAGCGGGTTACTAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCTGTc                                                                               <  1:8803428/101‑1 (MQ=255)
                        ccGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTc                                                                               <  2:5576967/101‑1 (MQ=255)
                        ccGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTc                                                                               <  1:5072547/101‑1 (MQ=255)
                         cGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCg                                                                              <  1:1362040/101‑1 (MQ=255)
                         cGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCg                                                                              >  2:3991143/1‑101 (MQ=255)
                            cTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.c                                                                          >  1:3534581/1‑101 (MQ=255)
                             tGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.cc                                                                         >  1:643932/1‑101 (MQ=255)
                              ggTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.ccc                                                                        >  2:5255980/1‑101 (MQ=255)
                                tCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.ccccc                                                                      <  1:3338308/101‑1 (MQ=255)
                                 cAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTCACCCGCTGGCCCTGCCGGCGATCGGCG.ATCAGCGGCCGCT.cccccc                                                                     >  2:4619378/1‑101 (MQ=37)
                                 cAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.cccccc                                                                     >  1:5281260/1‑101 (MQ=255)
                                 cAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.cccccc                                                                     <  1:3785187/101‑1 (MQ=255)
                                 cAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGGCGCT.cccccc                                                                     >  2:3340431/1‑101 (MQ=255)
                                  aGCGGGTCACGAAGCGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCCCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCcg                                                                    <  2:2947388/101‑1 (MQ=255)
                                   gCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCcgc                                                                   >  1:6125055/1‑101 (MQ=255)
                                   gCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCcgc                                                                   >  1:3912944/1‑101 (MQ=255)
                                    cGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCcgcg                                                                  >  2:7219900/1‑101 (MQ=255)
                                        ttACCAACCGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCggggg                                                              <  1:7245847/101‑1 (MQ=255)
                                         tACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGc                                                             <  1:942916/101‑1 (MQ=255)
                                            gAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTg                                                          >  1:2712145/1‑101 (MQ=255)
                                            gAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTg                                                          >  1:6920203/1‑101 (MQ=255)
                                              caacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCT.CCCCCCGCGGGGGCGTGGATc                                                      <  2:9007872/94‑1 (MQ=2)
                                              caacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCT.CCCCCCGCGGGGGCGTGGATc                                                      <  2:4201638/94‑1 (MQ=2)
                                              caacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCT.CCCCCCGCGGGGGCGTGGATc                                                      >  2:4779827/8‑101 (MQ=2)
                                               cacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCg                                                     <  2:2644140/95‑1 (MQ=2)
                                                cGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATc                                                      <  2:5945096/101‑1 (MQ=255)
                                                acactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGa                                                    >  1:4165857/6‑101 (MQ=2)
                                                 ggCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCCGCGGGGGCGGGGATc                                                      >  1:6635171/1‑101 (MQ=255)
                                                 ggCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCg                                                     >  2:2746627/1‑101 (MQ=255)
                                                 ggCAGTCGCTCCACCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCg                                                     <  1:2170455/101‑1 (MQ=255)
                                                 cactgTCGCCCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGaa                                                   <  1:1724955/97‑1 (MQ=2)
                                                  gCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGa                                                    <  1:1133560/101‑1 (MQ=255)
                                                   cAGTCGCTCCCCCCGCGGGGGCGCGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.GTCATCGGTCGCT.CCCCCCGCGGGGGCGGGGATCgca                                                   >  2:9151525/1‑99 (MQ=38)
                                                    ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGaaa                                                  <  1:1003894/100‑1 (MQ=255)
                                                    cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGaaa                                                  <  2:7653796/100‑1 (MQ=255)
                                                    aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGaaa                                                  >  2:8305879/1‑101 (MQ=255)
                                                    aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGAGGTCGCT.CCCCCCGCGGGGGCGTGGATCGaaa                                                  >  2:1901025/1‑101 (MQ=255)
                                                    aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGGGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGaaa                                                  >  2:8548885/1‑101 (MQ=255)
                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAt                                               >  2:4983235/1‑101 (MQ=255)
                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGAT.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAt                                               >  2:1049363/1‑101 (MQ=255)
                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATc                                              >  1:4360968/1‑101 (MQ=255)
                                                         cTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGCAACATcg                                             >  1:5386277/1‑101 (MQ=255)
                                                         cTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATcg                                             >  2:3856665/1‑101 (MQ=255)
                                                         cTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATcg                                             <  2:4041514/101‑1 (MQ=255)
                                                         cTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATcg                                             <  2:543255/101‑1 (MQ=255)
                                                          tCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CGCCCCGCGGGGGCGTGGATCGAAACATcgc                                            >  1:5797535/1‑101 (MQ=255)
                                                              cccGCGGGGGCGTGGATCGAAACTGCGCCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCt                                        <  1:3463452/101‑1 (MQ=255)
                                                              cccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCt                                        >  1:7159936/1‑101 (MQ=255)
                                                               ccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCtt                                       <  2:5820205/101‑1 (MQ=255)
                                                                cgcgGGGGCGTGGATCGAAACTGCCCCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCttt                                      <  2:3557753/101‑1 (MQ=255)
                                                                 gcgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTg                                     <  1:1458596/101‑1 (MQ=255)
                                                                 gcgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTg                                     <  2:3653452/101‑1 (MQ=255)
                                                                     gggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCgg                                 >  1:819161/1‑101 (MQ=255)
                                                                       gCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTc                               <  1:2477371/101‑1 (MQ=255)
                                                                       gCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTc                               >  1:7920484/1‑101 (MQ=255)
                                                                         gTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGa                             >  1:2313833/1‑101 (MQ=255)
                                                                          tGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGAc                            <  2:2256760/101‑1 (MQ=255)
                                                                           ggATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGCCa                           >  2:7829844/1‑101 (MQ=255)
                                                                           ggATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACa                           <  1:4050882/101‑1 (MQ=255)
                                                                            gATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAg                          <  2:808237/101‑1 (MQ=255)
                                                                             aTCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAgg                         >  2:6675991/1‑101 (MQ=255)
                                                                               cGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCt                       <  1:6329333/101‑1 (MQ=255)
                                                                               cGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCt                       <  1:7616801/101‑1 (MQ=255)
                                                                                gAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTg                      >  1:8533355/1‑101 (MQ=255)
                                                                                   accgcccccGCTGGCCGTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTa                   <  2:8473692/95‑1 (MQ=255)
                                                                                   aCTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTa                   <  2:1287839/101‑1 (MQ=255)
                                                                                   aCTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTa                   >  1:4535561/1‑101 (MQ=255)
                                                                                     tGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAc                 <  1:7466785/101‑1 (MQ=255)
                                                                                      gcccccGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAAATCGCCGCTTTGGCGGTCGACAGGCTGGTAACg                <  1:4765459/98‑1 (MQ=255)
                                                                                         cccGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACgtgt             <  2:630624/101‑1 (MQ=255)
                                                                                           cGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCg           <  2:2844114/101‑1 (MQ=255)
                                                                                             cTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGGAACGTGTCggt         >  2:6988330/1‑99 (MQ=255)
                                                                                               ggCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcc       <  1:2769083/101‑1 (MQ=255)
                                                                                               ggCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcc       <  1:5534725/101‑1 (MQ=255)
                                                                                                gCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTccc      >  2:731126/1‑101 (MQ=255)
                                                                                                 cccTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccc     >  2:7607735/1‑101 (MQ=255)
                                                                                                   cTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccccc   <  1:6822538/101‑1 (MQ=255)
                                                                                                   cTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccccc   >  2:1690274/1‑101 (MQ=255)
                                                                                                    tGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCcg  <  2:4298233/101‑1 (MQ=255)
                                                                                                    |                                                                                                      
TTCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCG  >  NC_014034/1395312‑1395512

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG