Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,427 A→C 4.6% intergenic (+239/‑692) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,4270A→C 4.6% 38.2 ‑0.4 ‑0.9416intergenic (+239/‑692)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (10/9):  ref base (181/216):  total (191/225)
Fisher's exact test strand distribution p-value = 6.40e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.36e-01

Only 100 of 495 total aligned reads displayed.

CAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCG  >  NC_014034/1395327‑1395527
                                                                                                     |                                                                                                     
cAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggtgatcggtg.a                                                                                                       >  1:2682052/1‑101 (MQ=255)
  aTTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCggtgatcggtg.atc                                                                                                     <  2:8490231/101‑1 (MQ=255)
   tttACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCa                                                                                                    <  1:3222258/101‑1 (MQ=255)
     tACGCCGTCTGGTCAGCGGTTTACGAAACGGCAGTCGCT.CCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCg                                                                                                 <  2:4717352/101‑1 (MQ=255)
      acacCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCCGCg                                                                                                 >  2:1228013/1‑101 (MQ=255)
         ccGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTc                                                                                              <  1:5338580/101‑1 (MQ=255)
          cGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCg                                                                                             >  1:5466224/1‑101 (MQ=255)
          cGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCg                                                                                             >  2:3991143/1‑101 (MQ=255)
           gTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGc                                                                                            >  2:2301338/1‑101 (MQ=255)
           gTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGc                                                                                            >  2:6329093/1‑101 (MQ=255)
           gTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGCTGATCGGTC.ATCAGCGGTCGc                                                                                            >  1:9157702/1‑101 (MQ=255)
            tCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCt                                                                                           <  1:5852593/101‑1 (MQ=255)
             cTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.c                                                                                         >  1:3534581/1‑101 (MQ=255)
               ggTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.ccc                                                                                       >  2:6435837/1‑101 (MQ=255)
                 tCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.ccccc                                                                                     <  1:8737775/101‑1 (MQ=255)
                 tCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.ccccc                                                                                     <  1:3338308/101‑1 (MQ=255)
                  cAGCGGGTTACGAAACGGCAGTCGCTCCCCCCCCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.cccccc                                                                                    <  1:6259024/101‑1 (MQ=255)
                   aGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGACGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCcg                                                                                   >  2:6752789/1‑101 (MQ=255)
                     cGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCcgcg                                                                                 >  2:7219900/1‑101 (MQ=255)
                      gggTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCgg                                                                                >  2:1897394/1‑101 (MQ=255)
                       ggTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCCCggg                                                                               >  2:1003876/1‑101 (MQ=255)
                         ttACCAACCGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCggggg                                                                             <  1:7245847/101‑1 (MQ=255)
                           aCGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCg                                                                           >  1:4543762/1‑101 (MQ=255)
                            cGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCgg                                                                          >  1:2484293/1‑100 (MQ=255)
                            cGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGt                                                                          <  1:5256164/101‑1 (MQ=255)
                             gAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTg                                                                         >  1:3730622/1‑101 (MQ=255)
                             gAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTg                                                                         >  2:2749534/1‑101 (MQ=255)
                              aaaCGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTgg                                                                        <  1:2301705/101‑1 (MQ=255)
                               caacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCT.CCCCCCGCGGGGGCGTGGATc                                                                     <  1:2335757/94‑1 (MQ=2)
                                aacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCg                                                                    <  1:840602/95‑1 (MQ=2)
                                 cGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATc                                                                     <  2:4546118/101‑1 (MQ=255)
                                 acactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGa                                                                   >  1:4165857/6‑101 (MQ=2)
                                  ggCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCCGCGGGGGCGGGGATc                                                                     >  1:6635171/1‑101 (MQ=255)
                                  ggCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCg                                                                    <  1:6068716/101‑1 (MQ=255)
                                  ggCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCg                                                                    <  1:8897295/101‑1 (MQ=255)
                                   actgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGaaa                                                                 >  1:1753588/4‑101 (MQ=2)
                                    cAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGaa                                                                  >  2:2671168/1‑101 (MQ=255)
                                    cAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGaa                                                                  <  2:1439744/101‑1 (MQ=255)
                                    cAGGCGCTCCCCCCGCGGGGGCGTGGAGCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGaa                                                                  <  1:1253117/101‑1 (MQ=255)
                                     tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAct                                                               >  1:7922964/2‑100 (MQ=2)
                                     ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGaaa                                                                 <  1:1003894/100‑1 (MQ=255)
                                     cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGaaa                                                                 <  2:7653796/100‑1 (MQ=255)
                                     aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGaaa                                                                 >  2:8305879/1‑101 (MQ=255)
                                     aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGaaa                                                                 >  2:1972707/1‑101 (MQ=255)
                                      gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAc                                                                <  1:3609760/101‑1 (MQ=255)
                                       tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACa                                                               >  2:615895/1‑101 (MQ=255)
                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAAAt                                                              >  2:5409298/1‑101 (MQ=255)
                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATc                                                             <  1:6811177/101‑1 (MQ=255)
                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATc                                                             >  1:4360968/1‑101 (MQ=255)
                                          cTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATcg                                                            <  2:4041514/101‑1 (MQ=255)
                                            ccccccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATcgcc                                                          >  1:2112046/1‑101 (MQ=255)
                                               cccGCGGGGGCGTGGATCGAAACTGCGCCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCt                                                       <  1:3463452/101‑1 (MQ=255)
                                                ccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCtt                                                      <  2:33943/101‑1 (MQ=255)
                                                ccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCtt                                                      <  1:6470486/101‑1 (MQ=255)
                                                  gcgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTg                                                    <  2:3653452/101‑1 (MQ=255)
                                                   cgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTgg                                                   >  2:8582892/1‑101 (MQ=255)
                                                   cgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTgg                                                   >  2:7910811/1‑101 (MQ=255)
                                                    gggggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGc                                                  >  1:5773559/1‑101 (MQ=255)
                                                     ggggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCg                                                 >  1:2811660/1‑101 (MQ=255)
                                                     ggggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCg                                                 >  1:8041306/1‑101 (MQ=255)
                                                       ggCGTGGATCGAATCTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGt                                               <  2:8857589/101‑1 (MQ=255)
                                                         cGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCg                                             >  2:5076557/1‑101 (MQ=255)
                                                         cGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCg                                             <  1:716685/101‑1 (MQ=255)
                                                          gTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGa                                            >  2:8675819/1‑101 (MQ=255)
                                                           tGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGAc                                           <  2:9016185/101‑1 (MQ=255)
                                                           tGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGAc                                           >  2:6923081/1‑101 (MQ=255)
                                                            ggATCGAAACTGCCCCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACa                                          <  2:5523758/101‑1 (MQ=255)
                                                            ggATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGCCa                                          >  2:7829844/1‑101 (MQ=255)
                                                            ggATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACa                                          <  1:4050882/101‑1 (MQ=255)
                                                             gATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAg                                         <  2:808237/101‑1 (MQ=255)
                                                              aTCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAgg                                        >  1:7468508/1‑101 (MQ=255)
                                                              aTCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAgg                                        >  2:6675991/1‑101 (MQ=255)
                                                               tCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGc                                       >  1:6917677/1‑101 (MQ=255)
                                                                cGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCt                                      <  1:7619676/101‑1 (MQ=255)
                                                                 gAAACTGCCCCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTg                                     <  2:3573567/101‑1 (MQ=255)
                                                                 gAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTg                                     >  2:2899299/1‑101 (MQ=255)
                                                                  aaaCTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTgg                                    <  1:11225/101‑1 (MQ=255)
                                                                   aaCTGCCCCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGt                                   <  2:5834004/101‑1 (MQ=255)
                                                                   aaCTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGt                                   >  1:1868266/1‑101 (MQ=255)
                                                                       gcccccGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAAATCGCCGCTTTGGCGGTCGACAGGCTGGTAACg                               <  1:4765459/98‑1 (MQ=255)
                                                                        cccccGCTGGCCCCTCCGGTGATCGGTT.ATCCCGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACgt                              <  2:4255005/99‑1 (MQ=25)
                                                                         aCCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACgtg                             >  1:1363506/1‑101 (MQ=255)
                                                                             gCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTGACGCGTCGc                         >  1:8576059/1‑101 (MQ=255)
                                                                                ggCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcc                      <  2:7610105/101‑1 (MQ=255)
                                                                                 gCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTccc                     <  1:6354617/101‑1 (MQ=255)
                                                                                 gCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTccc                     >  2:731126/1‑101 (MQ=255)
                                                                                    cTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccccc                  <  2:3008291/101‑1 (MQ=255)
                                                                                    cTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccccc                  <  1:3868328/101‑1 (MQ=255)
                                                                                    cTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccccc                  >  2:1690274/1‑101 (MQ=255)
                                                                                     tGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCcg                 >  1:1565879/1‑101 (MQ=255)
                                                                                      gCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCcgc                >  1:4013788/1‑101 (MQ=255)
                                                                                      gCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCcgc                >  1:705083/1‑101 (MQ=255)
                                                                                              tcggtg.atcAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGt        >  2:2182117/1‑101 (MQ=255)
                                                                                               cggtg.atcAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTg       >  1:5092919/1‑101 (MQ=255)
                                                                                                 gtg.atcAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACTTGTCGCTTCCCCCGCGGGGGCGTggg     >  2:3777962/1‑100 (MQ=255)
                                                                                                 gtg.atcAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGa     <  2:5773324/101‑1 (MQ=255)
                                                                                                 gtg.atcAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGa     >  2:9163434/1‑101 (MQ=255)
                                                                                                   g.atcAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATc   <  2:49035/101‑1 (MQ=255)
                                                                                                   g.atcAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATc   >  2:3152809/1‑101 (MQ=255)
                                                                                                     atcAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCg  <  1:2746709/101‑1 (MQ=255)
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CAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCG  >  NC_014034/1395327‑1395527

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 28 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG