Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,430 A→C 4.1% intergenic (+242/‑689) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,4300A→C 4.1% 35.4 ‑0.4 ‑0.1458intergenic (+242/‑689)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (10/9):  ref base (204/235):  total (214/244)
Fisher's exact test strand distribution p-value = 6.44e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.73e-01

Only 100 of 488 total aligned reads displayed.

TTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA  >  NC_014034/1395330‑1395530
                                                                                                    |                                                                                                     
tttACCCCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCa                                                                                                       <  2:7943756/101‑1 (MQ=255)
tttACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCa                                                                                                       >  1:8738539/1‑101 (MQ=255)
  tACGCCGTCTGGTCAGCGGTTTACGAAACGGCAGTCGCT.CCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCg                                                                                                    <  2:4717352/101‑1 (MQ=255)
  tACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGc                                                                                                     >  1:1821164/1‑101 (MQ=255)
   acacCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCCGCg                                                                                                    >  2:1228013/1‑101 (MQ=255)
   acacCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCg                                                                                                    >  1:3637000/1‑101 (MQ=255)
     acCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGt                                                                                                  <  1:194578/101‑1 (MQ=255)
     acCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGt                                                                                                  <  2:6863594/101‑1 (MQ=255)
      ccGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTc                                                                                                 >  1:1698460/1‑101 (MQ=255)
      ccGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTc                                                                                                 <  1:5338580/101‑1 (MQ=255)
       cGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCg                                                                                                <  1:1362040/101‑1 (MQ=255)
       cGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCg                                                                                                >  1:5466224/1‑101 (MQ=255)
       cGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCg                                                                                                >  2:3991143/1‑101 (MQ=255)
       cGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCg                                                                                                <  2:3123404/101‑1 (MQ=255)
        gTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGCTGATCGGTCATCAGCGGTCGc                                                                                               >  1:9157702/1‑101 (MQ=255)
         tCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCt                                                                                              <  1:5852593/101‑1 (MQ=255)
          cTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.c                                                                                            >  1:80889/1‑101 (MQ=255)
           tGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.cc                                                                                           <  2:7260669/101‑1 (MQ=255)
            ggTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.ccc                                                                                          <  1:6248460/101‑1 (MQ=255)
               cAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.ccaccc                                                                                       <  1:3277256/101‑1 (MQ=255)
               cAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGGCGCT.cccccc                                                                                       >  2:3340431/1‑101 (MQ=255)
                aGCGGGTCACGAAGCGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCCCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCcg                                                                                      <  2:2947388/101‑1 (MQ=255)
                    ggTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCCCggg                                                                                  >  2:1003876/1‑101 (MQ=255)
                      ttACCAACCGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCggggg                                                                                <  1:7245847/101‑1 (MQ=255)
                       tACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGc                                                                               >  1:265352/1‑101 (MQ=255)
                        aCGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCg                                                                              >  1:4543762/1‑101 (MQ=255)
                         cGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGt                                                                             <  1:5256164/101‑1 (MQ=255)
                          gAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTg                                                                            >  1:2712145/1‑101 (MQ=255)
                            aaCGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGa                                                                          <  2:2681970/101‑1 (MQ=255)
                             aCGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGAt                                                                         >  1:3436474/1‑101 (MQ=255)
                             aCGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGAt                                                                         <  2:7689358/101‑1 (MQ=255)
                              cGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATc                                                                        <  2:4546118/101‑1 (MQ=255)
                               ggCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCCGCGGGGGCGGGGATc                                                                        >  1:6635171/1‑101 (MQ=255)
                               ggCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCg                                                                       <  1:8897295/101‑1 (MQ=255)
                                 cAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGaa                                                                     <  2:1439744/101‑1 (MQ=255)
                                 cAGGCGCTCCCCCCGCGGGGGCGTGGAGCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGaa                                                                     <  1:1253117/101‑1 (MQ=255)
                                  ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGaaa                                                                    <  1:1003894/100‑1 (MQ=255)
                                  aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGaaa                                                                    >  2:1813510/1‑101 (MQ=255)
                                  aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGaaa                                                                    >  1:8445777/1‑101 (MQ=255)
                                   gccGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAc                                                                   <  2:3768855/99‑1 (MQ=255)
                                    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACa                                                                  >  2:615895/1‑101 (MQ=255)
                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAt                                                                 <  1:4454001/101‑1 (MQ=255)
                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAAAt                                                                 >  2:5409298/1‑101 (MQ=255)
                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATc                                                                <  1:6811177/101‑1 (MQ=255)
                                       cTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATcg                                                               >  1:3466418/1‑101 (MQ=255)
                                       cTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATcg                                                               <  2:4041514/101‑1 (MQ=255)
                                             ccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCtt                                                         <  1:7645038/101‑1 (MQ=255)
                                             ccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCtt                                                         <  1:6470486/101‑1 (MQ=255)
                                              cgcgGGGGCGTGGATCGAAACTGCCCCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCttt                                                        <  2:3557753/101‑1 (MQ=255)
                                               gcgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTg                                                       <  1:1458596/101‑1 (MQ=255)
                                                cgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTgg                                                      >  2:8582892/1‑101 (MQ=255)
                                                cgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTgg                                                      >  2:2515714/1‑101 (MQ=255)
                                                 gggggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGc                                                     >  1:5773559/1‑101 (MQ=255)
                                                 gggggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGc                                                     >  2:847638/1‑101 (MQ=255)
                                                 gggggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGc                                                     <  1:3883890/101‑1 (MQ=255)
                                                  ggggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCg                                                    >  1:2399311/1‑101 (MQ=255)
                                                    ggCGTGGATCGAATCTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGt                                                  <  2:8857589/101‑1 (MQ=255)
                                                      cGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCg                                                >  2:5076557/1‑101 (MQ=255)
                                                      cGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCg                                                >  2:207302/1‑101 (MQ=255)
                                                      cGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCg                                                <  1:716685/101‑1 (MQ=255)
                                                       gTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGa                                               >  1:2313833/1‑101 (MQ=255)
                                                       gTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGa                                               >  2:8675819/1‑101 (MQ=255)
                                                        tGGATCGAAACTGCCCCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGAc                                              <  2:7858023/101‑1 (MQ=255)
                                                        tGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGAc                                              <  2:2256760/101‑1 (MQ=255)
                                                        tGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGAc                                              <  2:9016185/101‑1 (MQ=255)
                                                         ggATCGAAACTGCCCCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACa                                             <  2:5523758/101‑1 (MQ=255)
                                                         ggATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACa                                             >  2:6348823/1‑101 (MQ=255)
                                                         ggATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACa                                             >  2:6487851/1‑101 (MQ=255)
                                                         ggATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACa                                             >  1:376701/1‑101 (MQ=255)
                                                          gATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAg                                            <  2:808237/101‑1 (MQ=255)
                                                           aTCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAgg                                           >  1:7468508/1‑101 (MQ=255)
                                                            tCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGc                                          >  1:6917677/1‑101 (MQ=255)
                                                               aaaCTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTgg                                       <  1:11225/101‑1 (MQ=255)
                                                                aaCTGCCCCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGt                                      <  2:5834004/101‑1 (MQ=255)
                                                                    gcccccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAAAATCGCCGCTTTGGCGGTCGACAGGCTGGTAACg                                  <  1:4765459/98‑1 (MQ=255)
                                                                     cccccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACgt                                 <  2:1904181/99‑1 (MQ=255)
                                                                     cccccGCTGGCCCCTCCGGTGATCGGTTATCCCGGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACgt                                 <  2:4255005/99‑1 (MQ=25)
                                                                        ccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCCGGGAACgggtg                              >  1:4270898/1‑100 (MQ=255)
                                                                         cGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCg                             <  2:2844114/101‑1 (MQ=255)
                                                                          gCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGc                            >  1:4045051/1‑101 (MQ=255)
                                                                           cTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGGAACGTGTCggt                           >  2:6988330/1‑99 (MQ=255)
                                                                            tGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTc                          >  1:3557764/1‑101 (MQ=255)
                                                                             ggCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcc                         <  1:2769083/101‑1 (MQ=255)
                                                                             ggCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcc                         <  2:7610105/101‑1 (MQ=255)
                                                                              gCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTccc                        >  2:731126/1‑101 (MQ=255)
                                                                                  cgCCGGTGATCAGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCcg                    <  2:3645174/100‑1 (MQ=255)
                                                                                   gCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCcgc                   >  1:705083/1‑101 (MQ=255)
                                                                                         gatcggtgatcAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGc             >  1:4120957/1‑101 (MQ=255)
                                                                                            cggtgatcAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTg          >  1:5092919/1‑101 (MQ=255)
                                                                                              gtgatcAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGa        >  2:9163434/1‑101 (MQ=255)
                                                                                              gtgatcAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGa        <  2:5773324/101‑1 (MQ=255)
                                                                                               tgatcAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGAt       >  1:217175/1‑101 (MQ=255)
                                                                                                gatcAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCTCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATc      >  1:6036381/1‑101 (MQ=255)
                                                                                                gatcAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATc      <  2:49035/101‑1 (MQ=255)
                                                                                                 atcAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCg     >  2:6713361/1‑101 (MQ=255)
                                                                                                 atcAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCg     >  1:6333596/1‑101 (MQ=255)
                                                                                                  tcAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGa    <  1:3119142/101‑1 (MQ=255)
                                                                                                   cAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGaa   >  2:8247507/1‑101 (MQ=255)
                                                                                                   cAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGaa   >  1:8601297/1‑101 (MQ=255)
                                                                                                    aGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa  >  1:7978424/1‑101 (MQ=255)
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TTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA  >  NC_014034/1395330‑1395530

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG