BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,395,474	T→G	18.0%	intergenic (+286/‑645)	RCAP_rcc01306 → / → RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,395,474	0	T→G	18.0%	236.8 ‑0.3 0.0	499	intergenic (+286/‑645)	RCAP_rcc01306/RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (42/49): ref base (197/211): total (239/260)
Fisher's exact test strand distribution p-value = 7.29e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 520 total aligned reads displayed.


CCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCC  >  NC_014034/1395374‑1395574


cccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..Ct                                                                                                       >  1:7159936/1‑101 (MQ=255)


 ccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..Ct.t                                                                                                     <  1:7645038/101‑1 (MQ=255)


  cgcgGGGGCGTGGATCGAAACTGCCCCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..Ct.tt                                                                                                    <  2:3557753/101‑1 (MQ=255)


   gcgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTg                                                                                                   <  2:2528516/101‑1 (MQ=255)


    cgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTgg                                                                                                  >  2:8582892/1‑101 (MQ=255)


     gggggGGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGc                                                                                                 <  1:2327255/101‑1 (MQ=255)


     gggggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGc                                                                                                 >  2:847638/1‑101 (MQ=255)


            tGGATCGAAACTGCCCCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGAc                                                                                          <  1:5427123/101‑1 (MQ=255)


            tGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGAc                                                                                          <  2:730642/101‑1 (MQ=255)


            tGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGAc                                                                                          >  2:2280321/1‑101 (MQ=255)


             ggATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACa                                                                                         >  1:376701/1‑101 (MQ=255)


             ggATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACa                                                                                         >  2:6348823/1‑101 (MQ=255)


             ggATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACa                                                                                         >  2:6487851/1‑101 (MQ=255)


               aTCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAgg                                                                                       >  1:7468508/1‑101 (MQ=255)


                    aaCTGCCCCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGt                                                                                  <  2:5834004/101‑1 (MQ=255)


                     aCTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTa                                                                                 <  2:1287839/101‑1 (MQ=255)


                      cTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTaa                                                                                <  1:3965134/101‑1 (MQ=255)


                        gCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACg                                                                              >  1:3497587/1‑101 (MQ=255)


                        gCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACg                                                                              <  2:612233/101‑1 (MQ=255)


                        gCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACg                                                                              <  1:1221939/101‑1 (MQ=255)


                        gCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACg                                                                              <  2:3694929/101‑1 (MQ=255)


                         cACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGGAACgt                                                                             >  1:4129292/1‑101 (MQ=255)


                           cccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACgtgt                                                                           <  1:2484454/101‑1 (MQ=255)


                           cccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACgtgt                                                                           <  1:18147/101‑1 (MQ=255)


                            ccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTc                                                                          <  1:5856007/101‑1 (MQ=255)


                            ccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTc                                                                          <  1:1006881/101‑1 (MQ=255)


                              gCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGc                                                                        >  1:4045051/1‑101 (MQ=255)


                                tGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTc                                                                      <  2:577560/101‑1 (MQ=255)


                                tGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGGAACGTGTCGCTc                                                                      >  2:3216240/1‑101 (MQ=255)


                                 ggCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTcc                                                                     <  2:7610105/101‑1 (MQ=255)


                                 ggCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTcc                                                                     <  1:2769083/101‑1 (MQ=255)


                                 ggCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTcc                                                                     >  1:4561526/1‑101 (MQ=255)


                                      tGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCcg                                                                >  1:1565879/1‑101 (MQ=255)


                                      tGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCcg                                                                <  2:4298233/101‑1 (MQ=255)


                                      cgCCGGTGATCAGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCcg                                                                <  2:3645174/100‑1 (MQ=255)


                                        ccGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCcgcg                                                              >  1:5320042/1‑101 (MQ=255)


                                           gtgatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCgggg                                                           >  1:2862823/1‑101 (MQ=255)


                                           gtgatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCgggg                                                           <  2:5508702/101‑1 (MQ=255)


                                           gtgatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGCGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCCCCCCCCGCgggg                                                           <  1:6556575/101‑1 (MQ=255)


                                                cggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTg                                                      >  1:5092919/1‑101 (MQ=255)


                                                cggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTg                                                      <  2:7981741/101‑1 (MQ=255)


                                                 ggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTgg                                                     >  2:4823053/1‑101 (MQ=255)


                                                  gctggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG..GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                   >  2:1794642/10‑101 (MQ=2)


                                                  gctggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG..GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                   <  1:5712136/92‑1 (MQ=2)


                                                   ctggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG..GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGGGGATc                                                  >  1:2105526/9‑101 (MQ=2)


                                                    tggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG..GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                 <  1:8619637/94‑1 (MQ=2)


                                                    tggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG..GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                 >  1:8105804/8‑101 (MQ=2)


                                                    tggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG..GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                 <  1:6770131/94‑1 (MQ=2)


                                                    tggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG..GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGGGGATCg                                                 >  1:4938259/8‑101 (MQ=2)


                                                     ggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG..GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATAGa                                                >  2:3975223/7‑101 (MQ=2)


                                                     atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                 >  2:6713361/1‑101 (MQ=255)


                                                         gtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG..GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                            >  2:72639/3‑101 (MQ=2)


                                                           ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG..GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACagc                                          <  1:3293409/101‑3 (MQ=2)


                                                           ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                           <  1:6027888/101‑1 (MQ=255)


                                                           ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGGGGCTCGAAACAt                                           >  2:8976857/1‑101 (MQ=255)


                                                            gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtc                                          <  2:2804436/101‑1 (MQ=255)


                                                             tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG..GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctt                                        >  1:6318290/1‑100 (MQ=2)


                                                             gcGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtct                                         <  1:2671250/100‑1 (MQ=255)


                                                              cGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG..GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACAgcttt                                       >  2:8132949/1‑99 (MQ=2)


                                                               gccccccccGCGGGGGCGTGGATCGAAACTACACCG..GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttc                                      <  1:7788790/98‑4 (MQ=0)


                                                               gCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCg                                       >  2:6916261/1‑101 (MQ=255)


                                                                cTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG..GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttca                                     <  2:33900/101‑5 (MQ=2)


                                                                 tCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCggg                                     >  2:9056260/1‑100 (MQ=255)


                                                                 tCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGt                                     <  1:448935/101‑1 (MQ=255)


                                                                 tCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGt                                     <  2:8738134/101‑1 (MQ=255)


                                                                   cccccGCGGGGGCGTGGATCGAAACTACACCG..GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaatg                                  <  1:831349/101‑8 (MQ=2)


                                                                     cccGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCg                                 <  2:7764576/101‑1 (MQ=255)


                                                                     cccGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCg                                 <  2:7864697/101‑1 (MQ=255)


                                                                      ccGCGGGGGCGTGGATCGAAACACCCCGGACCTGTCGGCGG.CGA.ACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtcgatgatcaa                                 <  1:6389223/101‑10 (MQ=0)


                                                                       cgcgGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTCTCGGTGGCGTc                               >  2:1367559/1‑101 (MQ=255)


                                                                        gcgGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCg                              <  1:8005695/101‑1 (MQ=255)


                                                                         cgGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGc                             >  1:3019490/1‑101 (MQ=255)


                                                                           ggggCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTc                           >  2:4617521/1‑101 (MQ=255)


                                                                               ggTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTcc                       <  2:4156515/100‑1 (MQ=255)


                                                                               cGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTcc                       >  1:4746214/1‑101 (MQ=255)


                                                                                gTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCa                      >  2:8798040/1‑101 (MQ=255)


                                                                                gTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCa                      >  1:4373998/1‑101 (MQ=255)


                                                                                gTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCa                      <  2:2168785/101‑1 (MQ=255)


                                                                                 tGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATTTCGGGGCCGTCGCTCGTCCAg                     >  1:9129070/1‑101 (MQ=255)


                                                                                 tGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAg                     <  2:5174377/101‑1 (MQ=255)


                                                                                  ggATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGc                    <  1:1329052/101‑1 (MQ=255)


                                                                                  ggATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGc                    >  1:966265/1‑101 (MQ=255)


                                                                                  ggATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGc                    >  2:7435126/1‑101 (MQ=255)


                                                                                     tCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCgg                 >  1:5154656/1‑101 (MQ=255)


                                                                                       gAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGcc               <  2:910809/101‑1 (MQ=255)


                                                                                       gAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGcc               <  2:199542/101‑1 (MQ=255)


                                                                                       gAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGcc               <  1:6488102/101‑1 (MQ=255)


                                                                                         aaCATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTg             <  1:8676204/101‑1 (MQ=255)


                                                                                         aaCATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTg             >  1:6159532/1‑101 (MQ=255)


                                                                                          aCATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGGCCAGCCGGCCTGc            >  2:5044136/1‑101 (MQ=255)


                                                                                           cATCGCGG..CT.TTGGCGGTGGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCt           <  1:8977278/101‑1 (MQ=255)


                                                                                           cATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCt           <  1:221034/101‑1 (MQ=255)


                                                                                            aTCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCtc          <  2:1625772/101‑1 (MQ=255)


                                                                                            aTCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCtc          >  1:8521109/1‑101 (MQ=255)


                                                                                            aTCGCCG..CT.TTGGCGATCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCtc          >  1:7257468/1‑101 (MQ=255)


                                                                                              cgccg..cT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCtcgt        >  1:6912365/1‑101 (MQ=255)


                                                                                              cgccg..cT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCtcgt        >  1:358467/1‑101 (MQ=255)


                                                                                               gccg..cT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCtcgtc       >  1:138237/1‑101 (MQ=255)


                                                                                                     cT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTc   <  2:1551433/101‑1 (MQ=255)


                                                                                                     cT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTc   >  1:7935480/1‑101 (MQ=255)


CCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCC  >  NC_014034/1395374‑1395574

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 29 ≤


ATCG

< 34 ≤


ATCG

< 40 ≤


ATCG