BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,395,477	G→T	18.5%	intergenic (+289/‑642)	RCAP_rcc01306 → / → RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,395,477	0	G→T	18.5%	251.2 ‑0.7 0.0	496	intergenic (+289/‑642)	RCAP_rcc01306/RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (42/50): ref base (201/203): total (243/253)
Fisher's exact test strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 518 total aligned reads displayed.


GCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCC  >  NC_014034/1395377‑1395577


gcgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTg                                                                                                      <  2:8128363/101‑1 (MQ=255)


 cgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTgg                                                                                                     >  2:8582892/1‑101 (MQ=255)


 cgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTgg                                                                                                     >  2:7910811/1‑101 (MQ=255)


  gggggGGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGc                                                                                                    <  1:2327255/101‑1 (MQ=255)


  gggggCGTGGATCGAAACTGCCCCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGc                                                                                                    <  1:2749616/101‑1 (MQ=255)


  gggggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGc                                                                                                    >  1:5773559/1‑101 (MQ=255)


    gggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCgg                                                                                                  >  1:819161/1‑101 (MQ=255)


       cGTGGATCGAAACNGCACCCGCNGGCNCTGNCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCg                                                                                               <  1:3285208/101‑1 (MQ=255)


         tGGATCGAAACTGCCCCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGAc                                                                                             <  2:7858023/101‑1 (MQ=255)


         tGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGAc                                                                                             <  1:4256315/101‑1 (MQ=255)


         tGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGAc                                                                                             <  2:2256760/101‑1 (MQ=255)


         tGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGAc                                                                                             >  1:8891807/1‑101 (MQ=255)


         gggATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGAc                                                                                             <  1:802163/100‑1 (MQ=255)


          ggATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACa                                                                                            <  2:7288596/101‑1 (MQ=255)


          ggATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACa                                                                                            >  1:376701/1‑101 (MQ=255)


           gATCGAAACTGCCCCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAg                                                                                           <  2:4066409/101‑1 (MQ=255)


           gATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAg                                                                                           <  1:8323856/101‑1 (MQ=255)


            aTCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAgg                                                                                          >  2:6675991/1‑101 (MQ=255)


                aaaCTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTgg                                                                                      >  1:5106944/1‑101 (MQ=255)


                 aaCTGCCCCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGt                                                                                     <  2:5834004/101‑1 (MQ=255)


                 aaCTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGt                                                                                     >  1:910822/1‑101 (MQ=255)


                  accgcccccGCTGGCCGTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTa                                                                                    <  2:8473692/95‑1 (MQ=255)


                  aCTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTa                                                                                    >  1:4535561/1‑101 (MQ=255)


                   cTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTaa                                                                                   <  1:3965134/101‑1 (MQ=255)


                     gCACCCGCTGGCCCTGCCGGTGATCGGTTATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGTAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACg                                                                                 >  1:6525000/1‑101 (MQ=255)


                     gCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACg                                                                                 <  2:3694929/101‑1 (MQ=255)


                     gCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACg                                                                                 >  1:5282248/1‑101 (MQ=255)


                     gCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACg                                                                                 <  1:1221939/101‑1 (MQ=255)


                     gCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGGAACg                                                                                 >  2:4818934/1‑101 (MQ=255)


                       ccccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACgtg                                                                               <  1:6321692/100‑1 (MQ=255)


                       aCCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACgtg                                                                               >  2:5122251/1‑101 (MQ=255)


                        cccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACgtgt                                                                              <  2:630624/101‑1 (MQ=255)


                        cccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACgtgt                                                                              <  1:2484454/101‑1 (MQ=255)


                         ccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTc                                                                             <  1:1006881/101‑1 (MQ=255)


                           gCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGc                                                                           >  1:4045051/1‑101 (MQ=255)


                               gCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTccc                                                                       <  2:3264334/101‑1 (MQ=255)


                               gCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTccc                                                                       >  1:3496777/1‑101 (MQ=255)


                                cccTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccc                                                                      >  2:7607735/1‑101 (MQ=255)


                                  cTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccccc                                                                    <  2:3008291/101‑1 (MQ=255)


                                  cTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccccc                                                                    >  2:1690274/1‑101 (MQ=255)


                                   tGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCcg                                                                   >  1:1565879/1‑101 (MQ=255)


                                   cgCCGGTGATCAGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCcg                                                                   <  2:3645174/100‑1 (MQ=255)


                                    gCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCcgc                                                                  >  1:2667378/1‑101 (MQ=255)


                                        gtgatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCgggg                                                              <  2:9157280/101‑1 (MQ=255)


                                        gtgatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCgggg                                                              >  1:2862823/1‑101 (MQ=255)


                                          gatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGc                                                            <  2:2027817/101‑1 (MQ=255)


                                          gatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGc                                                            >  1:4120957/1‑101 (MQ=255)


                                           atcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCg                                                           >  1:7727501/1‑101 (MQ=255)


                                              ggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTgg                                                        <  2:4610049/101‑1 (MQ=255)


                                              cgctggacgtggTCGCTCCCCCCGCGGGGGTGTGGATCGAAACTACACCG..GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGa                                                       <  1:7897654/91‑1 (MQ=2)


                                               gtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGa                                                       <  2:5773324/101‑1 (MQ=255)


                                                tgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGAt                                                      <  1:7220204/101‑1 (MQ=255)


                                                ggatcAGCGGTCGCGCCCCGCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCGCCCCCCGCGGGGGCGTGGAt                                                      <  1:7387187/100‑1 (MQ=255)


                                                ctggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG..GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                     >  1:5335307/9‑101 (MQ=0)


                                                ctggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG..GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGGGGATc                                                     >  1:2105526/9‑101 (MQ=2)


                                                 tggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG..GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                    >  2:275063/8‑101 (MQ=2)


                                                 gatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATc                                                     >  1:6036381/1‑101 (MQ=255)


                                                  ggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG..GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                   >  1:4587074/7‑101 (MQ=2)


                                                   gacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG..GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                  <  2:1324150/96‑1 (MQ=2)


                                                     aGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                 >  1:7978424/1‑101 (MQ=255)


                                                     aGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                 <  1:6923366/101‑1 (MQ=255)


                                                      gtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG..GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGGTGGAAGCa                                               >  2:4265272/3‑101 (MQ=2)


                                                        ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                              >  2:184960/1‑101 (MQ=255)


                                                        ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                              >  2:3966104/1‑101 (MQ=255)


                                                        ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGGGGCTCGAAACAt                                              >  2:8976857/1‑101 (MQ=255)


                                                         gTCGCGCCCCCCGCGGGGGCGTGGATCGAAACTACACCG..GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgct                                            <  2:1044225/101‑1 (MQ=2)


                                                          tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG..GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctt                                           <  2:1425766/101‑2 (MQ=2)


                                                          tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtct                                            <  1:9186831/101‑1 (MQ=255)


                                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtctc                                           <  1:1813563/101‑1 (MQ=255)


                                                            gCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCg                                          >  2:6916261/1‑101 (MQ=255)


                                                             cTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG..GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttca                                        >  2:6072326/1‑97 (MQ=2)


                                                             cTCCCCCCGCGGGGGCGTGGATCGAAACTACACCG..GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttca                                        <  2:5465576/101‑5 (MQ=2)


                                                              tCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCggg                                        >  2:9056260/1‑100 (MQ=255)


                                                              tCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGt                                        <  2:8738134/101‑1 (MQ=255)


                                                               ccccccGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTg                                       <  1:6195585/101‑1 (MQ=255)


                                                                 ccccGCGGGGGCGTGGATCGAAACTACACCG..GG.GCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaatgg                                    <  1:484645/101‑9 (MQ=2)


                                                                  cccGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCg                                    <  2:7764576/101‑1 (MQ=255)


                                                                   ccGCGGGGGCGTGGATCGAAACACCCCGGACCTGTCGGCGG.CGA.ACACCGATACCGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtcgatgatcaa                                    <  1:6389223/101‑10 (MQ=0)


                                                                     gcgGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGGGCCGTCg                                 >  2:161489/1‑101 (MQ=255)


                                                                      cgGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGc                                >  1:3019490/1‑101 (MQ=255)


                                                                       gggggCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCt                               >  1:8665399/1‑101 (MQ=255)


                                                                         gggCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCg                             >  1:7569269/1‑101 (MQ=255)


                                                                           gCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTc                           >  1:3567291/1‑101 (MQ=255)


                                                                           gCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTc                           >  1:4414197/1‑101 (MQ=255)


                                                                            cGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTcc                          <  2:3466314/101‑1 (MQ=255)


                                                                            cGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCCGCGCTCGTcc                          >  1:4945909/1‑101 (MQ=255)


                                                                             gTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCa                         <  2:7411876/101‑1 (MQ=255)


                                                                             gTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCa                         >  2:8798040/1‑101 (MQ=255)


                                                                             gTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCa                         >  1:548174/1‑101 (MQ=255)


                                                                              tGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAg                        >  1:6809802/1‑101 (MQ=255)


                                                                               ggATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGc                       <  1:1329052/101‑1 (MQ=255)


                                                                                gATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGcc                      <  1:8583277/101‑1 (MQ=255)


                                                                                   cGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGc                   >  2:4970901/1‑101 (MQ=255)


                                                                                    gAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGGCGCTCGTCCACTCGGcc                  >  2:2322229/1‑101 (MQ=255)


                                                                                        cATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCt              <  1:221034/101‑1 (MQ=255)


                                                                                           cgccg..cT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCtcgt           <  1:5878758/101‑1 (MQ=255)


                                                                                            gccg..cT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCtcgtc          >  1:138237/1‑101 (MQ=255)


                                                                                            gccg..cT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCtcgtc          <  2:643922/101‑1 (MQ=255)


                                                                                                   t.ttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTGGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTcc     >  1:6572113/1‑101 (MQ=255)


                                                                                                      tGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTcccc   >  2:7183367/1‑101 (MQ=255)


GCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCG..CT.TTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCC  >  NC_014034/1395377‑1395577

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 28 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG