Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,479 C→T 18.3% intergenic (+291/‑640) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,4790C→T18.3% 255.2 ‑0.4 ‑0.0497intergenic (+291/‑640)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (42/49):  ref base (200/206):  total (242/255)
Fisher's exact test strand distribution p-value = 6.43e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.22e-01

Only 100 of 515 total aligned reads displayed.

GGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCG  >  NC_014034/1395379‑1395579
                                                                                                    |                                                                                                    
gggggGGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGc                                                                                                      <  1:2327255/101‑1 (MQ=255)
gggggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGc                                                                                                      >  1:5773559/1‑101 (MQ=255)
gggggCGTCGATCGAATCTGCGCCCGCTGGGCCTGCCGGTGATCGGTGATCAGCGGTCGGTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGc                                                                                                      <  1:9151947/101‑1 (MQ=38)
     cGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCg                                                                                                 <  1:716685/101‑1 (MQ=255)
     cGTGGATCGAAACNGCACCCGCNGGCNCTGNCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCg                                                                                                 <  1:3285208/101‑1 (MQ=255)
       tGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGAc                                                                                               >  2:2280321/1‑101 (MQ=255)
       tGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGAc                                                                                               >  1:2065831/1‑101 (MQ=255)
        ggATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGAcg                                                                                              >  2:7879826/1‑100 (MQ=255)
        ggATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACa                                                                                              >  1:376701/1‑101 (MQ=255)
         gATCGAAACTGCCCCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAg                                                                                             <  2:4066409/101‑1 (MQ=255)
           tCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGc                                                                                           >  1:6917677/1‑101 (MQ=255)
            cGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCt                                                                                          <  2:7478509/101‑1 (MQ=255)
             gAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTg                                                                                         >  2:2899299/1‑101 (MQ=255)
                aCTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTa                                                                                      <  2:1287839/101‑1 (MQ=255)
                aCTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTa                                                                                      >  1:4535561/1‑101 (MQ=255)
                 cTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTaa                                                                                     <  1:3965134/101‑1 (MQ=255)
                  tGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAc                                                                                    <  2:3458749/101‑1 (MQ=255)
                   gCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACg                                                                                   >  1:3497587/1‑101 (MQ=255)
                   gCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACg                                                                                   >  1:5282248/1‑101 (MQ=255)
                   gCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACg                                                                                   <  1:1221939/101‑1 (MQ=255)
                   gCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACg                                                                                   <  2:3694929/101‑1 (MQ=255)
                    cACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGGAACgt                                                                                  >  1:4129292/1‑101 (MQ=255)
                     ccccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACgtg                                                                                 <  1:6321692/100‑1 (MQ=255)
                      cccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACgtgt                                                                                <  1:2484454/101‑1 (MQ=255)
                       ccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTc                                                                               <  1:1006881/101‑1 (MQ=255)
                       ccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCCGGGAACgggtg                                                                               >  1:4270898/1‑100 (MQ=255)
                         gCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGc                                                                             >  1:4045051/1‑101 (MQ=255)
                          cTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGGAACGTGTCggt                                                                            >  2:6988330/1‑99 (MQ=255)
                           tGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTc                                                                           <  1:4426694/101‑1 (MQ=255)
                            ggCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcc                                                                          <  1:2769083/101‑1 (MQ=255)
                             gCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTccc                                                                         >  2:731126/1‑101 (MQ=255)
                             gCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTccc                                                                         <  1:6354617/101‑1 (MQ=255)
                                cTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccccc                                                                      <  2:8602628/101‑1 (MQ=255)
                                 tGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCcg                                                                     <  2:6391801/101‑1 (MQ=255)
                                  gCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCcgc                                                                    >  1:2667378/1‑101 (MQ=255)
                                    cggtgatcggtgatCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGTgg                                                                  >  2:58239/1‑101 (MQ=255)
                                    cggtgatcggtgatCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCgg                                                                  >  2:6195346/1‑101 (MQ=255)
                                      gtgatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCgggg                                                                <  2:9157280/101‑1 (MQ=255)
                                      gtgatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGCGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCCCCCCCCGCgggg                                                                <  1:6556575/101‑1 (MQ=255)
                                         atcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCg                                                             >  1:8986748/1‑101 (MQ=255)
                                            ggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTgg                                                          >  2:4823053/1‑101 (MQ=255)
                                            ggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTgg                                                          <  2:4610049/101‑1 (MQ=255)
                                            cgctggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGa                                                         >  2:5002965/11‑101 (MQ=2)
                                             gtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGa                                                         <  2:5773324/101‑1 (MQ=255)
                                              tgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGAt                                                        <  2:2671251/101‑1 (MQ=255)
                                              ctggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                       >  1:5335307/9‑101 (MQ=0)
                                               tggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                      <  1:6770131/94‑1 (MQ=2)
                                               tggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                      <  1:8619637/94‑1 (MQ=2)
                                               gatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATc                                                       >  1:6036381/1‑101 (MQ=255)
                                                ggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                     >  1:4587074/7‑101 (MQ=2)
                                                ggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                     >  1:726806/7‑101 (MQ=2)
                                                   aGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                   >  1:2984334/1‑101 (MQ=255)
                                                    gtggTCGCTCCCCCCGCGGGGGCGTGGATCGATACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                 <  2:6752633/99‑1 (MQ=2)
                                                    gtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGGTGGAAGCa                                                 >  2:4265272/3‑101 (MQ=2)
                                                    gtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                 >  2:72639/3‑101 (MQ=2)
                                                      ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                >  2:3966104/1‑101 (MQ=255)
                                                      ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGGGGCTCGAAACAt                                                >  2:8976857/1‑101 (MQ=255)
                                                       gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtc                                               <  1:1593071/101‑1 (MQ=255)
                                                         gcccccccccGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtctc                                             <  2:6634911/97‑1 (MQ=255)
                                                         cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtctc                                             <  1:1813563/101‑1 (MQ=255)
                                                         cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtctc                                             <  2:5466039/101‑1 (MQ=255)
                                                         cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtctc                                             >  1:8168283/1‑101 (MQ=255)
                                                         cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAtctc                                             <  2:8469131/101‑1 (MQ=255)
                                                           cTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttca                                          >  2:6072326/1‑97 (MQ=2)
                                                           cTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCgg                                           <  2:3220184/101‑1 (MQ=255)
                                                            tCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCggg                                          >  2:9056260/1‑100 (MQ=255)
                                                            tCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGt                                          <  2:8738134/101‑1 (MQ=255)
                                                             ccccccGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTg                                         <  1:6195585/101‑1 (MQ=255)
                                                               ccccGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaatgg                                      <  1:484645/101‑9 (MQ=2)
                                                               ccccGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaatgg                                      >  1:7494102/1‑93 (MQ=2)
                                                               ccccGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGcc                                       >  1:8011934/1‑101 (MQ=255)
                                                                  cgcgGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTCTCGGTGGCGTc                                    >  2:1367559/1‑101 (MQ=255)
                                                                    cgGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGc                                  >  2:7642898/1‑101 (MQ=255)
                                                                     gggggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCt                                 >  1:2849082/1‑101 (MQ=255)
                                                                          cGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTcc                            <  1:5122420/101‑1 (MQ=255)
                                                                          cGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCCGCGCTCGTcc                            >  1:4945909/1‑101 (MQ=255)
                                                                           gTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCa                           >  1:548174/1‑101 (MQ=255)
                                                                           gTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCa                           <  2:2168785/101‑1 (MQ=255)
                                                                           gTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCa                           >  2:8798040/1‑101 (MQ=255)
                                                                             ggATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGc                         <  1:1329052/101‑1 (MQ=255)
                                                                             ggATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGc                         >  1:770838/1‑101 (MQ=255)
                                                                             ggATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCCAGTCCAGc                         >  2:7805585/1‑101 (MQ=255)
                                                                                 cGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGc                     <  2:5161117/101‑1 (MQ=255)
                                                                                  gAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGcc                    <  1:6488102/101‑1 (MQ=255)
                                                                                  gAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGcc                    <  2:199542/101‑1 (MQ=255)
                                                                                    aaCATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTg                  <  1:8676204/101‑1 (MQ=255)
                                                                                      cATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCt                <  1:221034/101‑1 (MQ=255)
                                                                                       aTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCtc               <  2:1625772/101‑1 (MQ=255)
                                                                                       aTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCtc               >  1:8521109/1‑101 (MQ=255)
                                                                                         cgccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCtcgt             >  1:358467/1‑101 (MQ=255)
                                                                                         cgccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCtcgt             <  1:5878758/101‑1 (MQ=255)
                                                                                          gccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCtcgtc            >  1:138237/1‑101 (MQ=255)
                                                                                          gccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCtcgtc            >  2:1006205/1‑101 (MQ=255)
                                                                                           ccgcTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCtcgtcg           <  2:1810746/101‑1 (MQ=255)
                                                                                              cTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTc        >  1:7935480/1‑101 (MQ=255)
                                                                                              cTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTc        <  2:1551433/101‑1 (MQ=255)
                                                                                               tttGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTcc       >  2:3634490/1‑101 (MQ=255)
                                                                                                  ggCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTccccc    <  1:7830177/101‑1 (MQ=255)
                                                                                                   gCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTcccccc   <  1:831375/101‑1 (MQ=255)
                                                                                                    cGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCcg  >  2:8063016/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
GGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCGTCGCTCCCCCCG  >  NC_014034/1395379‑1395579

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG